Online citations, reference lists, and bibliographies.
← Back to Search

Optoelectrofluidic Enhanced Immunoreaction Based On Optically-induced Dynamic AC Electroosmosis.

Dongsik Han, J. Park
Published 2016 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
We report a novel optoelectrofluidic immunoreaction system based on electroosmotic flow for enhancing antibody-analyte binding efficiency on a surface-based sensing system. Two conventional indium tin oxide glass slides are assembled to provide a reaction chamber for a tiny volume of sample droplet (∼5 μL), in which the top layer is employed as an antibody-immobilized substrate and the bottom layer acts as a photoconductive layer of an optoelectrofluidic device. Under the application of an AC voltage, an illuminated light pattern on the photoconductive layer causes strong counter-rotating vortices to transport analytes from the bulk solution to the vicinity of the assay spot on the glass substrate. This configuration overcomes the slow immunoreaction problem of a diffusion-based sensing system, resulting in the enhancement of binding efficiency via an optoelectrofluidic method. Furthermore, we investigate the effect of optically-induced dynamic AC electroosmotic flow on optoelectrofluidic enhancement for surface-based immunoreaction with a mathematical simulation study and real experiments using immunoglobulin G (IgG) and anti-IgG. As a result, dynamic light patterns provided better immunoreaction efficiency than static light patterns due to effective mass transport of the target analyte, resulting in an achievement of 2.18-fold enhancement under a growing circular light pattern compared to the passive mode.
This paper references
10.1016/0021-9797(92)90248-K
The binding of antigen by immobilized antibody: Influence of a variable adsorption rate coefficient on external diffusion limited kinetics
A. Sadana (1992)
10.1021/AC026016K
Microarrays for the screening of allergen-specific IgE in human serum.
B. I. Fall (2003)
10.1038/nature03831
Massively parallel manipulation of single cells and microparticles using optical images
P. Y. Chiou (2005)
10.1063/1.2996277
Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image
Wonjae Choi (2008)
10.1021/ac504360d
Biosensor Enhancement Using Grooved Micromixers: Part II, Experimental Studies.
N. S. Lynn (2015)
10.1006/JCIS.1999.6346
AC Electric-Field-Induced Fluid Flow in Microelectrodes.
Ramos (1999)
10.1109/JSEN.2015.2475610
Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics
Marwa Selmi (2015)
10.1039/b811740c
Rapid and selective concentration of microparticles in an optoelectrofluidic platform.
H. Hwang (2009)
10.1063/1.2981195
Simulation on binding efficiency of immunoassay for a biosensor with applying electrothermal effect
K. Huang (2008)
10.1016/j.bios.2012.12.041
A label-free impedimetric DNA sensing chip integrated with AC electroosmotic stirring.
C. Wu (2013)
10.1002/elps.200700415
Interactive manipulation of blood cells using a lens‐integrated liquid crystal display based optoelectronic tweezers system
H. Hwang (2008)
10.1002/PMIC.200300600
Protein microarrays: Promising tools for proteomic research
M. Templin (2003)
Analysis Of Transport Phenomena
W. Deen (1998)
10.1016/j.bios.2010.09.007
Improving immunosensor performances using an acoustic mixer on droplet microarray.
F. Kardous (2010)
10.1039/c1lc20277d
In situ dynamic measurements of the enhanced SERS signal using an optoelectrofluidic SERS platform.
H. Hwang (2011)
10.1021/ac101325t
Optoelectrofluidic sandwich immunoassays for detection of human tumor marker using surface-enhanced Raman scattering.
H. Hwang (2010)
10.1063/1.4790622
Optoelectrofluidic behavior of metal–polymer hybrid colloidal particles
Dongsik Han (2013)
10.1063/1.3253411
Generation and manipulation of droplets in an optoelectrofluidic device integrated with microfluidic channels
D. Lee (2009)
10.1021/ac901047v
Dynamic light-activated control of local chemical concentration in a fluid.
H. Hwang (2009)
10.1021/AC025777K
Three-dimensional microfluidic confinement for efficient sample delivery to biosensor surfaces. application to immunoassays on planar optical waveguides.
O. Hofmann (2002)
10.1063/1.3086600
Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force.
H. Hwang (2009)
10.1002/PMIC.200390009
Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers
J. Miller (2003)
10.1038/nmeth1109
Microarray-based genomic selection for high-throughput resequencing
D. Okou (2007)
10.1038/nbt1388
Making it stick: convection, reaction and diffusion in surface-based biosensors
T. Squires (2008)
10.1039/c3an00190c
A rapid electrochemical biosensor based on an AC electrokinetics enhanced immuno-reaction.
I. Cheng (2013)



This paper is referenced by
10.1039/D0SM01084G
Regulating the aggregation of colloidal particles in an electro-osmotic micropump.
Zhu Zhang (2020)
10.1063/1.5116737
Optoelectrokinetics-based microfluidic platform for bioapplications: A review of recent advances.
Wenfeng Liang (2019)
10.1016/j.aca.2019.02.004
Advancement of electroosmotic pump in microflow analysis: A review.
L. Li (2019)
10.3390/mi8070212
Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
Qingming Hu (2017)
10.1021/ACS.LANGMUIR.6B02637
Numerical Study of the Electrothermal Effect on the Kinetic Reaction of Immunoassays for a Microfluidic Biosensor.
Marwa Selmi (2016)
10.1016/J.SNB.2019.05.087
The effect of operating conditions on the optically induced electrokinetic (OEK)-based manipulation of magnetic microbeads in a microfluidic system
Jia-Long Hong (2019)
10.1063/1.4950787
Microarray-integrated optoelectrofluidic immunoassay system.
Dongsik Han (2016)
10.1021/acs.analchem.6b04281
Recent Advances in Electrochemical Immunosensors.
Wei Wen (2017)
10.1016/J.APPLTHERMALENG.2016.05.132
Electrothermal effect on the immunoassay in a microchannel of a biosensor with asymmetrical interdigitated electrodes
Marwa Selmi (2016)
10.1016/j.bios.2016.10.009
Scaffolds for oriented and close-packed immobilization of immunoglobulins.
M. Iijima (2017)
10.1038/s41598-017-06204-0
Optimization of microfluidic biosensor efficiency by means of fluid flow engineering
Marwa Selmi (2017)
10.1039/c9lc00975b
Sensitive tear screening of diabetic retinopathy with dual biomarkers enabled using a rapid electrokinetic patterning platform.
Jen-Yi Wang (2019)
10.1016/j.bios.2016.11.014
Detection of low-abundance biomarker lipocalin 1 for diabetic retinopathy using optoelectrokinetic bead-based immunosensing.
J. Wang (2017)
10.1039/C7CE01573A
Rapid synthesis of hierarchical, flower-like Ag microstructures with a gemini surfactant as a directing agent for SERS applications
Y. Xia (2017)
10.1002/elps.201700375
Review: Electric field driven pumping in microfluidic device
M. R. Hossan (2018)
10.1063/1.4982946
Particle concentrating and sorting under a rotating electric field by direct optical-liquid heating in a microfluidics chip.
Y. Chen (2017)
Semantic Scholar Logo Some data provided by SemanticScholar