Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Controlling The Orientation Of A Cell-synthesized Extracellular Matrix By Using Engineered Gelatin-based Building Blocks.

F. A. Pennacchio, C. Casale, F. Urciuolo, G. Imparato, R. Vecchione, P. Netti
Published 2018 · Materials Science, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
In tissue engineering there is growing interest in fabricating highly engineered platforms designed to instruct cells towards the synthesis of tissues that reproduce their natural counterpart. In this context, a fundamental factor to take into account is the control over the final tissue orientation, especially for what concerns the replication of load-bearing tissues whose functions are strictly related to their microstructural organization. Starting from this point, in this work we have engineered a gelatin-based hydrogel in order to be patterned by 2-photon polymerization (2PP) lithography for the fabrication of instructive free standing building blocks designed to produce anisotropic collagen-based μtissues. Biological results clearly highlighted the strong relationship between μtissue orientation and such topographies, which resulted in a crucial element in the production of highly anisotropic μtissues.
This paper references
10.1073/pnas.0801866105
Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
Y. Du (2008)
10.1002/ADFM.201203880
Hydrogels for Two-Photon Polymerization: A Toolbox for Mimicking the Extracellular Matrix
J. Torgersen (2013)
10.1002/CPLU.201500179
"On-Off" RGD Signaling Using Azobenzene Photoswitch-Modified Surfaces.
E. Vaselli (2015)
10.1126/science.1116995
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
D. Discher (2005)
10.1016/j.yjmcc.2009.08.024
The extracellular matrix: at the center of it all.
S. Bowers (2010)
10.1002/ADMA.201303233
25th anniversary article: Rational design and applications of hydrogels in regenerative medicine.
Nasim Annabi (2014)
10.1088/1758-5090/8/1/015010
Biophysical properties of dermal building-blocks affects extra cellular matrix assembly in 3D endogenous macrotissue.
F. Urciuolo (2016)
10.1039/B814285H
Modular Tissue Engineering: Engineering Biological Tissues from the Bottom Up.
J. W. Nichol (2009)
10.1007/B94405
Two-photon photopolymerization and 3D lithographic microfabrication
H. Sun (2004)
10.1021/LA9713006
Measuring the Elastic Properties of Thin Polymer Films with the Atomic Force Microscope
J. Domke (1998)
10.1002/ADFM.201404531
Microfluidic Spinning of Cell‐Responsive Grooved Microfibers
Xuetao Shi (2015)
10.1016/j.nano.2010.01.009
Nanotopographical modification: a regulator of cellular function through focal adhesions.
Manus Jonathan Paul Biggs (2010)
10.1002/ADFM.200400212
Acrylate-Based Photopolymer for Two-Photon Microfabrication and Photonic Applications†
L. Nguyen (2005)
10.1006/EXCR.1997.3625
Sensitivity of fibroblasts and their cytoskeletons to substratum topographies: topographic guidance and topographic compensation by micromachined grooves of different dimensions.
C. Oakley (1997)
10.1016/j.biomaterials.2009.09.012
Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules.
T. Dang (2009)
10.1021/nl503737k
Nanoengineered surfaces for focal adhesion guidance trigger mesenchymal stem cell self-organization and tenogenesis.
M. Iannone (2015)
10.1016/j.addr.2012.08.015
Surface engineering of inorganic nanoparticles for imaging and therapy.
Jutaek Nam (2013)
10.1002/bit.25298
A microgroove patterned multiwell cell culture plate for high‐throughput studies of cell alignment
Petra B Lücker (2014)
10.1038/nprot.2016.123
A decade of progress in tissue engineering
A. Khademhosseini (2016)
10.1016/j.drudis.2014.10.018
Cell-laden microfibers for bottom-up tissue engineering.
H. Onoe (2015)
10.1016/J.BIOMATERIALS.2006.02.024
Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy.
Todd Courtney (2006)
10.1167/IOVS.06-1216
Morphologic characterization of organized extracellular matrix deposition by ascorbic acid-stimulated human corneal fibroblasts.
Xiaoqing Guo (2007)
10.1089/ten.TEA.2011.0606
Anisotropic fibrous scaffolds for articular cartilage regeneration.
S. Mccullen (2012)
10.1242/dmm.004077
Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer
T. Cox (2011)
10.1021/bm1015305
Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue engineering.
A. Ovsianikov (2011)
10.14797/MDCJ-9-4-213
Building a tissue in vitro from the bottom up: implications in regenerative medicine.
F. Urciuolo (2013)
10.1002/anie.200805179
Engineering substrate topography at the micro- and nanoscale to control cell function.
C. Bettinger (2009)
10.1002/adma.201301762
Cell-material interactions revealed via material techniques of surface patterning.
X. Yao (2013)
10.1016/J.BIOMATERIALS.2007.07.021
Microengineered hydrogels for tissue engineering.
A. Khademhosseini (2007)
10.1016/j.biomaterials.2010.03.064
Cell-laden microengineered gelatin methacrylate hydrogels.
J. W. Nichol (2010)
10.1038/nmat2208
Guided and fluidic self-assembly of microstructures using railed microfluidic channels.
S. E. Chung (2008)
10.1042/BJ3160001
Collagen fibril formation.
K. Kadler (1996)
10.1039/b804234a
Stop-flow lithography to generate cell-laden microgel particles.
P. Panda (2008)
10.1016/J.EURPOLYMJ.2015.05.014
An overview of injectable polymeric hydrogels for tissue engineering
A. Sivashanmugam (2015)
10.1529/BIOPHYSJ.106.093740
Second harmonic generation confocal microscopy of collagen type I from rat tendon cryosections.
T. Theodossiou (2006)
10.1016/S0021-9290(01)00145-2
Collagen fibril diameters increase and fibril densities decrease in skin subjected to repetitive compressive and shear stresses.
J. Sanders (2001)
10.1016/j.biotechadv.2015.05.007
Towards the design of 3D multiscale instructive tissue engineering constructs: Current approaches and trends.
Sara M. Oliveira (2015)
10.1016/j.addr.2012.07.014
Nanotopography-guided tissue engineering and regenerative medicine.
H. Kim (2013)
10.1021/acsami.7b13176
Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System.
Fabrizio Andrea Pennacchio (2018)
10.1016/j.biomaterials.2013.06.062
The role of microscaffold properties in controlling the collagen assembly in 3D dermis equivalent using modular tissue engineering.
G. Imparato (2013)
10.1016/S0142-9612(02)00175-8
Photopolymerizable hydrogels for tissue engineering applications.
K. Nguyen (2002)
10.1042/BJ0110001
Occurrence of Raffinose in the Seed of the Jute Plant (Corchorus capsularis).
H. E. Annett (1917)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar