Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Controlling The Orientation Of A Cell-synthesized Extracellular Matrix By Using Engineered Gelatin-based Building Blocks.
F. A. Pennacchio, C. Casale, F. Urciuolo, G. Imparato, R. Vecchione, P. Netti
Published 2018 · Materials Science, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
In tissue engineering there is growing interest in fabricating highly engineered platforms designed to instruct cells towards the synthesis of tissues that reproduce their natural counterpart. In this context, a fundamental factor to take into account is the control over the final tissue orientation, especially for what concerns the replication of load-bearing tissues whose functions are strictly related to their microstructural organization. Starting from this point, in this work we have engineered a gelatin-based hydrogel in order to be patterned by 2-photon polymerization (2PP) lithography for the fabrication of instructive free standing building blocks designed to produce anisotropic collagen-based μtissues. Biological results clearly highlighted the strong relationship between μtissue orientation and such topographies, which resulted in a crucial element in the production of highly anisotropic μtissues.
This paper references
Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
Y. Du (2008)
Hydrogels for Two-Photon Polymerization: A Toolbox for Mimicking the Extracellular Matrix
J. Torgersen (2013)
"On-Off" RGD Signaling Using Azobenzene Photoswitch-Modified Surfaces.
E. Vaselli (2015)
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
D. Discher (2005)
The extracellular matrix: at the center of it all.
S. Bowers (2010)
25th anniversary article: Rational design and applications of hydrogels in regenerative medicine.
Nasim Annabi (2014)
Biophysical properties of dermal building-blocks affects extra cellular matrix assembly in 3D endogenous macrotissue.
F. Urciuolo (2016)
Modular Tissue Engineering: Engineering Biological Tissues from the Bottom Up.
J. W. Nichol (2009)
Two-photon photopolymerization and 3D lithographic microfabrication
H. Sun (2004)
Measuring the Elastic Properties of Thin Polymer Films with the Atomic Force Microscope
J. Domke (1998)
Microfluidic Spinning of Cell‐Responsive Grooved Microfibers
Xuetao Shi (2015)
Nanotopographical modification: a regulator of cellular function through focal adhesions.
Manus Jonathan Paul Biggs (2010)
Acrylate-Based Photopolymer for Two-Photon Microfabrication and Photonic Applications†
L. Nguyen (2005)
Sensitivity of fibroblasts and their cytoskeletons to substratum topographies: topographic guidance and topographic compensation by micromachined grooves of different dimensions.
C. Oakley (1997)
Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules.
T. Dang (2009)
Nanoengineered surfaces for focal adhesion guidance trigger mesenchymal stem cell self-organization and tenogenesis.
M. Iannone (2015)
Surface engineering of inorganic nanoparticles for imaging and therapy.
Jutaek Nam (2013)
A microgroove patterned multiwell cell culture plate for high‐throughput studies of cell alignment
Petra B Lücker (2014)
A decade of progress in tissue engineering
A. Khademhosseini (2016)
Cell-laden microfibers for bottom-up tissue engineering.
H. Onoe (2015)
Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy.
Todd Courtney (2006)
Morphologic characterization of organized extracellular matrix deposition by ascorbic acid-stimulated human corneal fibroblasts.
Xiaoqing Guo (2007)
Anisotropic fibrous scaffolds for articular cartilage regeneration.
S. Mccullen (2012)
Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer
T. Cox (2011)
Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue engineering.
A. Ovsianikov (2011)
Building a tissue in vitro from the bottom up: implications in regenerative medicine.
F. Urciuolo (2013)
Engineering substrate topography at the micro- and nanoscale to control cell function.
C. Bettinger (2009)
Cell-material interactions revealed via material techniques of surface patterning.
X. Yao (2013)
Microengineered hydrogels for tissue engineering.
A. Khademhosseini (2007)
Cell-laden microengineered gelatin methacrylate hydrogels.
J. W. Nichol (2010)
Guided and fluidic self-assembly of microstructures using railed microfluidic channels.
S. E. Chung (2008)
Collagen fibril formation.
K. Kadler (1996)
Stop-flow lithography to generate cell-laden microgel particles.
P. Panda (2008)
An overview of injectable polymeric hydrogels for tissue engineering
A. Sivashanmugam (2015)
Second harmonic generation confocal microscopy of collagen type I from rat tendon cryosections.
T. Theodossiou (2006)
Collagen fibril diameters increase and fibril densities decrease in skin subjected to repetitive compressive and shear stresses.
J. Sanders (2001)
Towards the design of 3D multiscale instructive tissue engineering constructs: Current approaches and trends.
Sara M. Oliveira (2015)
Nanotopography-guided tissue engineering and regenerative medicine.
H. Kim (2013)
Three-Dimensional Microstructured Azobenzene-Containing Gelatin as a Photoactuable Cell Confining System.
Fabrizio Andrea Pennacchio (2018)
The role of microscaffold properties in controlling the collagen assembly in 3D dermis equivalent using modular tissue engineering.
G. Imparato (2013)
Photopolymerizable hydrogels for tissue engineering applications.
K. Nguyen (2002)
Occurrence of Raffinose in the Seed of the Jute Plant (Corchorus capsularis).
H. E. Annett (1917)
This paper is referenced by
Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules
M. Battisti (2019)
Tailoring Cellular Function: The Contribution of the Nucleus in Mechanotransduction
Fabrizio A. Pennacchio (2020)
Cellular ageing of oral fibroblasts differentially modulates extracellular matrix organization.
Srividya Atkuru (2020)
Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering
Taotao Liu (2020)
Precision 3D-Printed Cell Scaffolds Mimicking Native Tissue Composition and Mechanics.
Amelie Erben (2020)
Hydrogel scaffolds for tissue engineering: the importance of polymer choice
C. Spicer (2020)
A material odyssey for 3D nano/microstructures: two photon polymerization based nanolithography in bioapplications
Caizhi Liao (2020)