Online citations, reference lists, and bibliographies.
← Back to Search

High-yield Graphene Produced From The Synergistic Effect Of Inflated Temperature And Gelatin Offers High Stability And Cellular Compatibility.

P. Tiwari, N. Kaur, V. Sharma, S. Mobin
Published 2018 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The direct exfoliation of graphite (Gr) is highly desirable and feasible compared to conventional processes owing to its non-oxidative, facile and controlled synthesis conditions. Herein, gelatin (gel), a hydrolysed form of collagen, was used as an exfoliant to directly exfoliate Gr. The main advantages of exploring gel as an exfoliant is its easy availability, low cost and high biocompatibility, which alleviate the drawbacks of previous exfoliation methods. The effect of the exfoliation parameters such as temperature, ratio of interacting species and pH of the solution offers a high yield of graphene (G) with the added advantages of good solubility, easy dispersibility and high stability. The temperature elevation caused by the dissipation of sonic waves facilitates a high exfoliation yield. Yield of 4.37 mg mL-1 of G was achieved under the conditions of 7 h sonication at 60 °C, pH 7 and Gr to gel ratio of 60 : 40, whereas yield of 1 mg mL-1 was achieved under sonication at 30 °C. Raman spectroscopy and transmission electron microscopy indicated the production of G sheets with 3-5 layers. The adsorption of gel on the surface of G via π-π interactions offers high stability and retains its inherent crystallinity. The as-synthesized G dispersion exhibits good cyto- and hemocompatibility. Unlike graphene oxide, the G dispersion does not affect RBCs at a relatively high concentration of 10 mg mL-1. These findings offer new avenues for the large-scale production of G and promote its biomedical applications, particularly in scaffold materials and intravenous drug delivery.
This paper references
10.1021/nl902200b
Solution phase production of graphene with controlled thickness via density differentiation.
A. Green (2009)
10.1016/J.CARBON.2013.12.062
Size-controlled synthesis of graphite nanoflakes and multi-layer graphene by liquid phase exfoliation of natural graphite
A. Alaferdov (2014)
10.1039/C3TA12212C
From graphite to graphene: direct liquid-phase exfoliation of graphite to produce single- and few-layered pristine graphene
Wencheng Du (2013)
10.1016/J.POLYMER.2010.11.042
Graphene-based polymer nanocomposites
J. R. Potts (2011)
10.1002/adma.201205157
Direct exfoliation of graphite to graphene in aqueous media with diazaperopyrenium dications.
S. Sampath (2013)
10.1021/acsami.5b07432
Direct Fabrication of the Graphene-Based Composite for Cancer Phototherapy through Graphite Exfoliation with a Photosensitizer.
G. Liu (2015)
10.1038/ncomms9294
Direct exfoliation and dispersion of two-dimensional materials in pure water via temperature control
Jinseon Kim (2015)
10.1039/C5TB00798D
Liposome-induced exfoliation of graphite to few-layer graphene dispersion with antibacterial activity.
R. Zappacosta (2015)
10.1016/J.CARBON.2015.03.045
Gelatin-assisted fabrication of graphene-based nacre with high strength, toughness, and electrical conductivity
M. Lian (2015)
10.1002/ADFM.201500016
In Situ Production of Biofunctionalized Few‐Layer Defect‐Free Microsheets of Graphene
A. M. Gravagnuolo (2015)
10.1016/J.SNB.2016.08.169
Cytocompatible peroxidase mimic CuO:graphene nanosphere composite as colorimetric dual sensor for hydrogen peroxide and cholesterol with its logic gate implementation
V. Sharma (2017)
10.1039/C5TB02065D
Direct exfoliation of graphite into graphene in aqueous solutions of amphiphilic peptides.
Meiwen Cao (2016)
10.1021/am302704a
Graphene oxide as a quencher for fluorescent assay of amino acids, peptides, and proteins.
Shanghao Li (2012)
10.1039/c3cp43205j
Critical parameters in exfoliating graphite into graphene.
Matat Buzaglo (2013)
10.1186/1556-276X-6-95
A novel mechanical cleavage method for synthesizing few-layer graphenes
B. Jayasena (2011)
10.1021/la303049w
Biomolecule-assisted, environmentally friendly, one-pot synthesis of CuS/reduced graphene oxide nanocomposites with enhanced photocatalytic performance.
Yingwei Zhang (2012)
10.1002/adma.201504760
Synthesis of Graphene Films on Copper Foils by Chemical Vapor Deposition.
X. Li (2016)
10.1038/s41467-017-02580-3
A non-dispersion strategy for large-scale production of ultra-high concentration graphene slurries in water
L. Dong (2017)
10.1021/ACS.IECR.5B03502
Solvent-Based Exfoliation via Sonication of Graphitic Materials for Graphene Manufacture
M. P. Lavin-Lopez (2016)
10.1007/s12274-013-0345-3
Enzyme-directed pH-responsive exfoliation and dispersion of graphene and its decoration by gold nanoparticles for use as a hybrid catalyst
K. Qu (2013)
10.1088/0957-4484/24/26/265703
Measuring the lateral size of liquid-exfoliated nanosheets with dynamic light scattering.
Mustafa Lotya (2013)
10.1247/CSF.7.245
The Effects on Cell Adhesion of Fibronectin and Gelatin in a Serum-Free, Bovine Serum Albumin Medium
M. Kan (1982)
10.1002/ADFM.201503247
Kitchen Chemistry 101: Multigram Production of High Quality Biographene in a Blender with Edible Proteins
A. Pattammattel (2015)
10.1038/srep36143
High-efficient Synthesis of Graphene Oxide Based on Improved Hummers Method
Huitao Yu (2016)
10.1002/anie.200903463
Carbon nanomaterials in biosensors: should you use nanotubes or graphene?
Wenrong Yang (2010)
10.1039/C1JM10749F
Green and facile synthesis of highly biocompatible graphene nanosheets and its application for cellular imaging and drug delivery
Kunping Liu (2011)
10.1002/CNMA.201600363
One‐Step Simultaneous Exfoliation and Covalent Functionalization of MoS2 by Amino Acid Induced Solution Processes
Elumalai Satheeshkumar (2017)
10.1016/j.colsurfb.2013.05.022
Graphene-based materials biocompatibility: a review.
A. M. Pinto (2013)
10.1002/adma.201203229
New horizons for diagnostics and therapeutic applications of graphene and graphene oxide.
Lingyan Feng (2013)
10.1021/jf500138f
Antioxidative, hemocompatible, fluorescent carbon nanodots from an "end-of-pipe" agricultural waste: exploring its new horizon in the food-packaging domain.
Manashi Das Purkayastha (2014)
Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing.
J. Carmichael (1987)
10.1021/cr300335p
Graphene: promises, facts, opportunities, and challenges in nanomedicine.
H. Y. Mao (2013)
10.1039/C4GC01752H
Synergy of oxygen and a piranha solution for eco-friendly production of highly conductive graphene dispersions
Keerthi Savaram (2015)
10.1021/JA00278A055
The sonochemical hot spot
K. Suslick (1986)
10.1016/j.ssc.2008.02.024
Ultrahigh electron mobility in suspended graphene
K. Bolotin (2008)
10.1038/nnano.2009.58
Chemical methods for the production of graphenes.
S. Park (2009)
10.1039/c4cp05864j
Scalable production of wrinkled and few-layered graphene sheets and their use for oil and organic solvent absorption.
D. Liu (2015)
10.1039/C5GC02455B
Eco-friendly production of high quality low cost graphene and its application in lithium ion batteries
A. Kamali (2016)
10.1038/AM.2017.11
A graphene-based chemical nose/tongue approach for the identification of normal, cancerous and circulating tumor cells
L. Wu (2017)
10.1038/nmat2166
Epitaxial graphene on ruthenium.
P. Sutter (2008)
10.1021/am200428v
Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts.
Ken-Hsuan Liao (2011)
10.1021/nl9016623
High-yield organic dispersions of unfunctionalized graphene.
C. Hamilton (2009)
10.1021/jacs.5b02780
Protein Induces Layer-by-Layer Exfoliation of Transition Metal Dichalcogenides.
Guijian Guan (2015)
10.1039/c7cs00363c
Promises, facts and challenges for graphene in biomedical applications.
G. Reina (2017)
10.1002/CNMA.201700055
Graphene/Polymer Nanocomposites for Supercapacitors
X. Zhang (2017)
10.1038/NNANO.2010.69
Erratum: Chemical methods for the production of graphenes
S. Park (2010)
10.1039/c6cp06813h
Scalable exfoliation and dispersion of two-dimensional materials - an update.
Hengcong Tao (2017)
10.1016/J.CARBON.2017.02.052
One-step exfoliation and functionalization of graphene by hydrophobin for high performance water molecular sensing
J. Tao (2017)
10.1016/J.CARBON.2014.11.036
Lignin-assisted direct exfoliation of graphite to graphene in aqueous media and its application in polymer composites
W. Liu (2015)
10.1039/C3RA45984E
A direct route towards preparing pH-sensitive graphene nanosheets with anti-cancer activity
D. Joseph (2014)
10.1021/am405378x
Double-stranded DNA-graphene hybrid: preparation and anti-proliferative activity.
D. Joseph (2014)
10.1002/anie.201001806
Interfacial engineering by proteins: exfoliation and functionalization of graphene by hydrophobins.
P. Laaksonen (2010)
10.1038/NMAT1849
The rise of graphene.
Andre K. Geim (2007)
10.1002/smll.201502207
Production of Two-Dimensional Nanomaterials via Liquid-Based Direct Exfoliation.
L. Niu (2016)
10.1039/C5RA06629H
Liquid phase collagen modified graphene that induces apoptosis
Soumya Bhattacharya (2015)
10.1038/nature04969
Graphene-based composite materials
S. Stankovich (2006)
10.1038/srep42258
Functionalized Graphene Oxide with Chitosan for Protein Nanocarriers to Protect against Enzymatic Cleavage and Retain Collagenase Activity
Fatemeh Emadi (2017)
10.1039/C2JM33173J
Gelatin-assisted fabrication of water-dispersible graphene and its inorganic analogues
Yu Ge (2012)
10.1039/c4nr07569b
Facile and green production of aqueous graphene dispersions for biomedical applications.
S. Ahadian (2015)
10.1016/j.addr.2016.04.009
Antibacterial applications of graphene-based nanomaterials: Recent achievements and challenges.
H. Ji (2016)
10.1016/J.APSUSC.2015.08.178
Bovine serum albumin bioconjugated graphene oxide: Red blood cell adhesion and hemolysis studied by QCM-D
B. Cai (2015)
10.1039/C6TB02086K
Graphene and graphene-based nanocomposites: biomedical applications and biosafety.
Satyanarayan Pattnaik (2016)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar