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A Study Of The Conditions And Mechanism Of The Diphenylamine Reaction For The Colorimetric Estimation Of Deoxyribonucleic Acid.

Kenneth Burton
Published 1956 · Chemistry, Medicine

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Of the colour reactions available for the determination and identification of deoxyribonucleic acid (DNA), the reaction with diphenylamine in a mixture of acetic and sulphuric acids at 1000 (Dische, 1930) has been perhaps the most widely used. The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature. As a result of this observation the procedure has been modified, principally by adding acetaldehyde to the reagents and by allowing the solution to stand for about 17 hr. at 30° instead of heating it at 1000. The modified method is 3-5 times as sensitive as Dische's original procedure, and several substances which interfere in the original method do not do so in the modified procedure. Some observations on the mechanism of the reaction have been made; in particular it was discovered that there is a liberation of inorganic orthophosphate from DNA during the early stages of the reaction. This finding has a bearing on the structure of DNA. The modified method has already been used in an investigation of nucleic acid metabolism during bacteriophage multiplication (Burton, 1955).
This paper references
Some chemical properties of desoxyribose nucleosides.
Manson La (1951)
10.1128/JB.68.4.483-492.1954
Quantification of the Feulgen reaction in bacteria.
N. Dondero (1954)
Arch
G. Rosen (1950)
10.1016/s0021-9258(18)71049-1
A sensitive method for the determination of deoxyribonucleic acid in tissues and microorganisms.
J. M. Webb (1955)
10.1016/s0021-9258(18)55739-2
Enzymatic synthesis and breakdown of desoxyribose phosphate.
E. Racker (1952)
10.1042/BJ0610473
The relation between the synthesis of deoxyribonucleic acid and the synthesis of protein in the multiplication of bacteriophage T2.
Kenneth Burton (1955)
Biochem
K. Academic Press Inc. Burton (1955)
10.1016/s0021-9258(19)50874-2
The formation apurinic acid from the desoxyribonucleic acid of calf thymus.
C. Tamm (1952)
10.1177/001452469000101110
"J."
G.G. Stokes (1890)
10.1042/BJ0320295
An improved method for the colorimetric determination of phosphate.
I. Berenblum (1938)
Biochem
K. Lohmann (1934)
kluyveri, which was isolated by Barker & Taha (1942) and used in the studies on fatty acid
of Cl (1942)
10.1042/BJ0540677
Efficiency of oxidative phosphorylation during the oxidation of pyruvate.
W. Bartley (1953)
Bull
G. Amiard (1948)
10.1038/168604A0
Separation of Deoxypentose and Pentose Nucleic Acids
S. Zamenhof (1951)
1951a) later observed that dried cells could oxidize butyrate and vinylacetate
vinylacetate. Kennedy (1951)
10.1016/s0021-9258(18)65414-6
The isolation of desoxyribonucleic acid from bacteriophages by an improved method.
V. L. Mayers (1954)
10.1016/s0021-9258(18)55583-6
A microchemical determination of desoxyribonucleic acid.
G. Ceriotti (1952)



This paper is referenced by
10.15369/SUJMS1989.15.201
Actions of Cytokines in Caerulein-induced Acute Pancreatitis in Mice
K. Kitamura (2003)
10.1111/J.1447-0756.1988.TB00075.X
Nuclear steroid receptors in endometrial cancers.
J. Fujimoto (1988)
10.1006/TAAP.1999.8760
Decrease in protein kinase and phosphatase activities in the liver nuclei of rats exposed to carbon tetrachloride.
M. Omura (1999)
10.1016/S0278-6915(03)00066-8
Tissue repair plays pivotal role in final outcome of liver injury following chloroform and allyl alcohol binary mixture.
S. S. Anand (2003)
10.2527/1999.772388X
Metabolism and morphology of brown adipose tissue from Brahman and Angus newborn calves.
G. Martin (1999)
10.1016/S0039-6060(98)70008-X
Adaptation after small bowel resection is attenuated by sialoadenectomy: the role for endogenous epidermal growth factor.
M. Helmrath (1998)
10.1089/THY.1998.8.1127
Nitric oxide donors inhibit iodide transport and organification and induce morphological changes in cultured bovine thyroid cells.
M. Costamagna (1998)
10.1515/cclm.1978.16.9.493
In Vitro Conversion of Estradiol-17β into Estrone in Normal Human Myometrium und Leiomyoma
K. Pollow (1978)
10.1002/1097-4644(20000915)78:4<541::AID-JCB3>3.0.CO;2-9
Role of endogenous regucalcin in the regulation of Ca2+‐ATPase activity in rat liver nuclei
Y. Tsurusaki (2000)
10.1016/S0742-8413(96)00093-X
Up-regulation of estrogen receptor mRNA and estrogen receptor activity by estradiol in liver of rainbow trout and other teleostean fish.
M. Mackay (1996)
10.1016/S0887-2333(96)00057-4
Effects of vitamin D on aortic smooth muscle cells in culture.
C. Tukaj (1996)
10.1254/JJP.32.369
Effect of malotilate (diisopropyl 1,3-dithiol-2-ylidenemalonate) on the protein synthesis in rat liver.
Y. Imaizumi (1982)
Thymus capitatus ) regulating altered hepatic mRNA expression of apoptotic genes during Nickel Chloride exposure
S. Linjawi (2010)
10.1016/j.biortech.2016.04.021
Heavy metals removal from wastewater using extracellular polymeric substances produced by Cloacibacterium normanense in wastewater sludge supplemented with crude glycerol and study of extracellular polymeric substances extraction by different methods.
K. Nouha (2016)
10.1038/bjc.1994.134
Modified multiple drug resistance phenotype of Chinese hamster ovary cells selected with X-rays and vincristine versus X-rays only.
S. McClean (1994)
10.1016/0006-2952(80)90194-X
A comparative study on the effects of α-hexachlorocyclohexane and its metabolite β-pentachlorocyclohexene on growth and monooxygenase activities in rat liver
W. Parzefall (1980)
10.1007/BF03349347
Evidence of specific nuclear binding sites for T3 in the mouse cultured fibroblast
A. Brisson-Lougarre (1979)
10.1016/0042-6822(79)90444-6
Vaccinia virus infection of HeLa cells. II. Disparity between cytoplasmic and nuclear viral-specific RNA.
A. Bolden (1979)
10.1007/BF01962878
The histochemistry of isocitric and oestradiol-17 β dehydrogenases in the endometrium of postmenopausal women treated with oestrogens and progestogens
G. Lane (2005)
10.1016/S0022-5347(17)41228-6
Enzyme studies in human transitional cell carcinoma of the urinary bladder.
I. Mitchell (1989)
10.1016/0014-5793(78)80818-7
Rapid antagonistic actions of noradrenaline and insulin on rat adipocyte phosphatidate phosphohydrolase activity
C. H. Cheng (1978)
10.1016/0006-2952(76)90010-1
Effect of diet-induced thiamine deficiency on visceral DNA synthesis and tissue composition.
G. Henderson (1976)
10.1016/0304-4165(78)90227-1
Acidic-phosphoprotein phosphatase activity of rat ventral prostate nuclei: apparent lack of effect of androgens.
M. Wilson (1978)
10.1016/0006-291X(77)91431-0
Enzyme-bound early product of purified poly(ADP-ribose) polymerase.
K. Yoshihara (1977)
10.1016/0014-4894(78)90105-4
Entamoeba histolytica, E. invadens, and E. moshkovskii: fluctuations of the DNA content of axenic trophozoites.
R. López-Revilla (1978)
10.1007/BF00330828
Evidence for a second “Prereplicative G2” repair mechanism, specific for γ-induced damage, in wild-type Schizosaccharomyces pombe
N. Gentner (2004)
10.1002/JCP.1040860304
DNA replication in homogenates of Physarum polycephalum
E. N. Brewer (1975)
10.1016/0090-6980(77)90174-5
Effects of prostaglandins E2 and F2alpha on lecithin biosynthesis by cultured lung cells.
G. Colacicco (1977)
10.1016/S0039-128X(75)80003-1
Characterization of a nuclear receptor for testosterone in rat bone marrow
L. Valladares (1975)
10.1038/262107a0
Relationship between receptor and mammary tumour virus production after stimulation by glucocorticoid
G. Shyamala (1976)
10.1016/0006-2952(74)90327-X
Biochemical studies of a new antitumor agent, O2,2'-cyclocytidine.
D. Ho (1974)
10.1038/262407a0
Role of oestrogen receptor in androgen-induced sexual differentiation of the brain
B. Westley (1976)
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