← Back to Search
Expression Of Alpha-amylases, Carbohydrate Metabolism, And Autophagy In Cultured Rice Cells Is Coordinately Regulated By Sugar Nutrient.
M. Chen, L. Liu, Y. Chen, H. K. Wu, S. Yu
Published 1994 · Medicine, Biology
Download PDFAnalyze on Scholarcy
A rice suspension cell culture system has been established to study how sugar depletion regulates alpha-amylase expression, carbohydrate metabolism, and other physiological and cellular changes. It is shown here that a group of 44 kDa alpha-amylases are constitutively expressed whether or not the cells are starved of sucrose. However, expression of a new group of alpha-amylases of 46 kDa is dramatically induced when cells are starved of sucrose. Cellular sugar and starch were rapidly consumed and metabolic activity was decreased in the starved cells. Extensive autophagy also occurred in the starved cells, which caused an increase in vacuolar volume and degradation of cytoplasmic constituents including amyloplasts. Immunocytochemical studies revealed that alpha-amylases are localized in starch granules within amyloplasts, in cell walls, and in some of the vacuoles. The presence of putative signal sequences in the N-termini of nine rice alpha-amylases suggests hitherto unidentified pathways for import of alpha-amylases into amyloplasts. The studies show that differential alpha-amylase expression, carbohydrate metabolism, metabolic activity, and vacuolar autophagy are coordinately regulated by the sugar level in the medium. As the starved suspension cells exhibit some sugar-regulated characteristics of alpha-amylase expression in germinating rice embryos as well as physiological changes similar to those in senescing cells, this system represents an ideal tool for studying cellular, biochemical, and molecular biological aspects of alpha-amylase gene regulation, carbohydrate metabolism, senescence, and protein targeting in plants.
This paper references
Metabolic derepression of alpha-amylase gene expression in suspension-cultured cells of rice.
S. M. Yu (1991)
P. Seglen (1990)
Sorting of Proteins in The Secretory System
M. Chrispeels (1991)
A new method for predicting signal sequence cleavage sites.
G. von Heijne (1986)
Metabolic regulation of rice alpha-amylase and sucrose synthase genes in planta.
E. Karrer (1992)
A new method for predicting signal sequence cleavage sites.
G. Heijne (1986)
Signal sequences. The limits of variation.
G. von Heijne (1985)
Light-Induced Chloroplast Differentiation in Soybean Cells in Suspension Culture : Ultrastructural Changes during the Bleaching and Greening Cycles.
M. Gillott (1991)
Protein translocation across membranes.
K. Verner (1988)
Refinement of the triphenyl tetrazolium chloride method of determining cold injury.
P. Steponkus (1967)
Antibodies: A Laboratory Manual
E. Harlow (1988)
Laboratory manual for physiological studies of rice
S. Yoshida (1971)
Expression and secretion of rice α-amylase by Saccharomyces cerevisiae
M. Kumagai (1990)
Control of transcription and mRNA turnover as mechanisms of metabolic repression of α‐amylase gene expression
J. Sheu (1994)
Light - induced chloroplast differentiation in soybean cells in suspension cul - Sucrose starvation of rice cells 635 ture
M. A. Gillott (1991)
Vacuolar localization of proteases and degradation of chloroplasts in mesophyll protoplasts from senescing primary wheat leaves.
V. A. Wittenbach (1982)
The Lytic Compartment of Plant Cells
Philippe Matile (1975)
Short peptide domains target proteins to plant vacuoles
M. Chrispeels (1992)
A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.
J. M. Neuhaus (1991)
Starch degradation. In Biochemistry of Plants, Volume 14 (Preiss, J., ed.)
M. Steup (1988)
Expression and secretion of rice (x-amylase by Saccharomyces
M. H. Kumagai (1990)
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
C. Merril (1981)
Immunohistochemical localization of barley stripe mosaic virions in infected wheat cells.
N. Lin (1983)
7 – Starch Degradation
M. Steup (1988)
Biosynthesis and Degradation of Starch in Higher Plants
E. Beck (1989)
The Transport of Proteins into Chloroplasts
C. Robinson (1986)
Molecular and Cellular Biology Associated with Endosperm Mobilization in Germinating Cereal Grains
G. Fincher (1989)
Regulation of alpha-amylase-encoding gene expression in germinating seeds and cultured cells of rice.
S. Yu (1992)
The transport of proteins into chloroplasts.
G. W. Schmidt (1986)
Signal sequences: The limits of variation
G. Vonheijne (1985)
Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction
K. Takeshige (1992)
Regulation of a-amylase-encoding
R. Wu (1992)
Novel gene expression system for plant cells based on induction of alpha-amylase promoter by carbohydrate starvation.
M. Chan (1994)
Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.
S. Lacks (1980)
This paper is referenced by
Production of human serum albumin by sugar starvation induced promoter and rice cell culture
Li-Fen Huang (2004)
Expression of genes encoding the rice translation initiation factor, eIF5A, is involved in developmental and environmental responses.
Wan-Chi Chou (2004)
Signal Peptide-Dependent Targeting of a Rice α-Amylase and Cargo Proteins to Plastids and Extracellular Compartments of Plant Cells1
Min-Huei Chen (2004)
The role of macroautophagy in the ageing process, anti-ageing intervention and age-associated diseases.
E. Bergamini (2004)
Successive amino-terminal proteolysis of the large subunit of ribulose 1,5-biphosphate carboxylase/oxygenase by vacuolar enzymes from French bean leaves.
T. Yoshida (1996)
Molecular Farming of Plants and Animals for Human and Veterinary Medicine
L. E. Erickson (2002)
Evidence for autophagy-dependent pathways of rRNA turnover in Arabidopsis
Brice E. Floyd (2015)
Regulation by sucrose of storage compounds breakdown in germinating seeds of yellow lupine (Lupinus luteus L.), white lupine (Lupinus albus L.) and Andean lupine (Lupinus mutabilis Sweet): I. Mobilization of storage protein
S. Borek (2011)
Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control1[W]
M. Nicolaï (2006)
Production of mouse granulocyte‐macrophage colony‐stimulating factor by gateway technology and transgenic rice cell culture
Yu-Kuo Liu (2012)
Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet
S. Borek (2009)
Molecular farming of pharmaceutical proteins
R. Fischer (2004)
3-methyladenine inhibits autophagy in tobacco culture cells under sucrose starvation conditions.
Chihiro Takatsuka (2004)
Transcriptome Profiling of the Response of Arabidopsis Suspension Culture Cells to Suc Starvation1[w]
Anthony L Contento (2004)
Characterisation of putative -amylases from apple (Malus domestica) and Arabidopsis thaliana
D. Stanley (2002)
Expression and characterisation of biopharmaceuticals in heterologous expression systems
A. Paetz (2005)
Kinetic modelling of the metabolic response of isolated lamb’s lettuce (Valerianella locusta L.) cells to sugar starvation
Victor Baiye Mfortaw Mbong (2017)
Sugar starvation induces the central vacuolation with coordinated increase in expression of tonoplast intrinsic protein genes in suspension-cultured rice cells
M. Lim (2016)
Novel Molecular Markers for Late Phases of the Growth Cycle of Arabidopsis thaliana Cell-Suspension Cultures Are Expressed during Organ Senescence
D. Callard (1996)
Cellular and genetic responses of plants to sugar starvation.
S. Yu (1999)
Running title: α-Amylase in Rice Chloroplasts Correspondence:
T. Mitsui (2005)
Multiple mode regulation of a cysteine proteinase gene expression in rice.
S. Ho (2000)
Développement d'une méthode d'isolation des noyaux adaptée aux macrophages pour une caractérisation protéomique d'une nouvelle structure autophagique induite par le virus HSV-1
T. Boukhris (2011)
Sugar coordinately and differentially regulates growth- and stress-related gene expression via a complex signal transduction network and multiple control mechanisms.
S. Ho (2001)
Genome-Wide Identification, Classification, and Expression Analysis of Autophagy-Associated Gene Homologues in Rice (Oryza sativa L.)
Kuaifei Xia (2011)
Production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture.
Jae-Wan Jung (2016)
Importance of Post-Translational Modifications for Functionality of a Chloroplast-Localized Carbonic Anhydrase (CAH1) in Arabidopsis thaliana
Stefan Burén (2011)
TRANSFORMATION OF TOMATO WITH Trichoderma ENDOCHITINASE (ech 42) CARRYING RICE α-AMYLASE SIGNAL PEPTIDE
A. Committee (2013)
Influence of the Starchy Endosperm on α-Amylase Isozyme Levels in Barley
R. Skadsen (1997)
Cellular and Genetic Responses of Plants to Sugar Starvation
Su-May Yu (1999)
Ultrastructural and enzymatic research on the role of sucrose in mobilization of storage lipids in germinating yellow lupine seeds
S. Borek (2006)
Transcriptomic adaptations in rice suspension cells under sucrose starvation
H. Wang (2006)See more