← Back to Search
Overexpression Of A Kinase-deficient Form Of The EDR1 Gene Enhances Powdery Mildew Resistance And Ethylene-induced Senescence In Arabidopsis.
Dingzhong Tang, R. Innes
Published 2002 · Medicine, Biology
Download PDFAnalyze on Scholarcy
The EDR1 gene of Arabidopsis has previously been reported to encode a Raf-like mitogen-activated protein kinase kinase (MAPKK) kinase, and to function as a negative regulator of disease resistance. A phylogenetic analysis of plant and animal protein kinases revealed, however, that plant Raf-like kinases are more closely related to animal mixed lineage kinases (MLKs) than Raf-like kinases, and are deeply divergent from both classes of animal kinases, making inferences of substrate specificity questionable. We, therefore, assayed the kinase activity of recombinant EDR1 protein in vitro. The EDR1 kinase domain displayed autophosphorylation activity and phosphorylated the common MAP kinase substrate myelin basic protein. The EDR1 kinase domain also phosphorylated a kinase-deficient EDR1 protein, indicating that EDR1 autophosphorylation can occur via an intermolecular mechanism. Overexpression of a kinase-deficient full-length EDR1 gene (35S::dnEDR1) in wild-type Arabidopsis plants caused a dominant negative phenotype, conferring resistance to powdery mildew (Erysiphe cichoracearum) and enhancing ethylene-induced senescence. RNA-gel blot analyses showed that the 35S::dnEDR1 transgene was highly transcribed in transgenic plants. Western blot analysis, however, revealed that neither the wild-type nor mutant EDR1 protein could be detected in these lines, indicating that the dominant negative phenotype may be caused by a translational inhibition mechanism rather than by a protein level effect. Overexpression of orthologous dnEDR1 constructs may provide a novel strategy for controlling powdery mildew disease in crops.
This paper references
RNA interference: It's a small RNA world
E. Moss (2001)
Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens.
B. P. Thomma (1998)
MLK‐3 activates the SAPK/JNK and p38/RK pathways via SEK1 and MKK3/6.
L. Tibbles (1996)
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
S. Altschul (1997)
Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1.
R. Leister (1996)
Requirement of functional ethyleneinsensitive 2 gene for efficient resistance of Arabidopsis to infection by Botrytis cinerea. Plant Phys
B P H J Thomma (1999)
The complexity of Raf-1 regulation.
D. Morrison (1997)
Salicylic acid has a role in regulating gene expression during leaf senescence.
K. Morris (2000)
Negative regulation of defense responses in plants by a conserved MAPKK kinase.
C. Frye (2001)
MicroRNAs in plants.
B. J. Reinhart (2002)
Plant MAP kinase kinase kinases structure, classification and evolution.
S. Jouannic (1999)
22 – RECOMBINANT PCR
R. Higuchi (1990)
Post-transcriptional silencing of a neomycin phosphotransferase II transgene correlates with the accumulation of unproductive RNAs and with increased cytosine methylation of 3' flanking regions
Helena Van Houdt (1997)
EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis.
J. Alonso (1999)
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
B. J. Reinhart (2000)
EDS1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases.
A. Falk (1999)
Plant mitogen-activated protein kinase signaling cascades.
G. Tena (2001)
Endogenous and Silencing-Associated Small RNAs in Plants Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003210.
C. Llave (2002)
Identification of Novel Genes Coding for Small Expressed RNAs
M. Lagos-Quintana (2001)
Mitogen-activated protein kinase cascades in plants: a new nomenclature.
Kazuya Ichimura (2002)
PAD4 Functions Upstream from Salicylic Acid to Control Defense Responses in Arabidopsis
N. Zhou (1998)
Plant MAP kinase kinase kinases
S. Jouannic (1999)
Recombinant PCR. In PCR Protocols, a Guide to Methods and Applications
R. Higuchi (1990)
Phosphorylation of myelin protein: recent advances.
J. Eichberg (1996)
Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in Arabidopsis.
K. Ichimura (1998)
Rpg1, a soybean gene effective against races of bacterial blight, maps to a cluster of previously identified disease resistance genes
T. Ashfield (1998)
An Arabidopsis Mutant with Enhanced Resistance to Powdery Mildew
C. Frye (1998)
Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental Timing
A. Grishok (2001)
Systemic Acquired Resistance
J. Ryals (1994)
Control of developmental timing by small temporal RNAs: a paradigm for RNA‐mediated regulation of gene expression
D. Banerjee (2002)
Requirement of functional ethylene-insensitive 2 gene for efficient resistance of Arabidopsis to infection by Botrytis cinerea.
B. P. Thomma (1999)
Biochemical Properties of Two Protein Kinases Involved in Disease Resistance Signaling in Tomato*
G. Sessa (1998)
Characteristics of post‐transcriptional gene silencing
A. Chicas (2001)
Coordinated plant defense responses in Arabidopsis revealed by microarray analysis.
P. Schenk (2000)
Mixed-Lineage Kinase 3 Delivers CD3/CD28-Derived Signals into the IκB Kinase Complex
S. Hehner (2000)
TAK1 Mediates the Ceramide Signaling to Stress-activated Protein Kinase/c-Jun N-terminal Kinase*
K. Shirakabe (1997)
Concomitant Activation of Jasmonate and Ethylene Response Pathways Is Required for Induction of a Plant Defensin Gene in Arabidopsis
I. Penninckx (1998)
Mixed-lineage kinase 3 delivers CD3/CD28-derived signals into the IkappaB kinase
S. P. Hehner (2000)
MAP kinase signalling cascade in Arabidopsis innate immunity
T. Asai (2002)
SA, JA, ethylene, and disease resistance in plants.
X. Dong (1998)
The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis.
R. Nishihama (2001)
Raf-1 protein kinase is required for growth of induced NIH/3T3 cells
W. Kolch (1991)
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF
A. Macnicol (1993)
A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA
G. Hutvágner (2001)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
D. Baulcombe (2002)
CTR1, a negative regulator of the ethylene response pathway in arabidopsis, encodes a member of the Raf family of protein kinases
J. Kieber (1993)
Mapping out the roles of MAP kinases in plant defense.
R. Innes (2001)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
J. Thompson (1994)
Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes
B. Quirino (2004)
Leaf senescence in Brassica napus: expression of genes encoding pathogenesis-related proteins
C. Hanfrey (2004)
ATMRK1, an Arabidopsis protein kinase related to mammal mixed-lineage kinases and Raf protein kinases
K. Ichimura (1997)
Salicylic acid activates a 48-kD MAP kinase in tobacco.
S. Zhang (1997)
Closing the ranks to attack by powdery mildew.
P. Schulze-Lefert (2000)
Plant MAP kinase kinase kinases
S. Jouannic (1999)
This paper is referenced by
Reassessing the MAP3K and MAP4K relationships.
A. Champion (2004)
The Pseudomonas syringae type III effector AvrRpt2 functions downstream or independently of SA to promote virulence on Arabidopsis thaliana.
Z. Chen (2004)
The Protein Phosphatases and Protein Kinases of Arabidopsis thaliana
H. Wang (2007)
Mitogen‐Activated Protein Kinase Cascades in Plant Signaling
Shuqun Zhang (2018)
Multiple intramolecular trafficking signals in RESISTANCE TO POWDERY MILDEW 8.2 are engaged in activation of cell death and defense
Yanyan Huang (2019)
Arabidopsis MEKK1 can take a short cut: it can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter
Ying Miao (2007)
Ascorbic acid deficiency in arabidopsis induces constitutive priming that is dependent on hydrogen peroxide, salicylic acid, and the NPR1 gene.
Madhumati Mukherjee (2010)
Biological Host Response: A Paradigm and Strategy to Overcome Biotic Stress Caused by Powdery Mildew Causal Agents in Plants
S. M. Bhosle (2020)
Functions of MAPK Cascade Pathways in Plant Defense Signaling
Y. Cheong (2010)
Arabidopsis thaliana Raf22 protein kinase maintains growth capacity during postgerminative growth arrest under stress.
J. Hwang (2018)
Regulation of plant defense responses in Arabidopsis by EDR2, a PH and START domain-containing protein.
Dingzhong Tang (2005)
Recent developments toward achieving fungal disease resistance in transgenic plants
Z. Punja (2006)
Emerging MAP kinase pathways in plant stress signalling.
H. Nakagami (2005)
Negative regulation of defence signalling pathways by the EDR1 protein kinase.
K. M. Christiansen (2011)
Powdery mildew susceptibility and biotrophic infection strategies.
R. Hückelhoven (2005)
A mutation in a coproporphyrinogen III oxidase gene confers growth inhibition, enhanced powdery mildew resistance and powdery mildew-induced cell death in Arabidopsis
Chuan-yu Guo (2013)
Mechanisms of plant innate immunity
N. Kozyrovska (2006)
Mutations in the EDR1 Gene Alter the Response of Arabidopsis thaliana to Phytophthora infestans and the Bacterial PAMPs flg22 and elf18.
K. Geissler (2015)
Molecular aspects of ageing and the onset of leaf senescence
Jozefus Hendrikus Maria Schippers (2008)
Cloning, overexpression, purification and preliminary X-ray analysis of the protein kinase domain of enhanced disease resistance 1 (EDR1) from Arabidopsis thaliana.
Heidi Kaljunen (2014)
The Powdery Mildew Disease of Arabidopsis: A Paradigm for the Interaction between Plants and Biotrophic Fungi
Cristina Micali (2008)
Suppression of edr2-mediated powdery mildew resistance, cell death and ethylene-induced senescence by mutations in ALD1 in Arabidopsis.
Haozhen Nie (2011)
Bioengineering and Molecular Manipulation of Mitogen-Activated Kinases to Activate Plant Innate Immunity for Crop Disease Management
P. Vidhyasekaran (2020)
START domains in lipid/sterol transfer and signaling in plants
B. P. Venkata (2006)
Host defence in a developmental context.
M. Whalen (2005)
Leaf senescence: signals, execution, and regulation.
Yongfeng Guo (2005)
The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling pathways
Bekir Ülker (2006)
Molecular characterization of mlo-based powdery mildew resistance and the role of heterotrimeric G-protein signaling in Arabidopsis defense
Justine Lorek (2011)
Wheat gene TaS3 contributes to powdery mildew susceptibility
Shaohui Li (2013)
A mutation in the GTP hydrolysis site of Arabidopsis dynamin-related protein 1E confers enhanced cell death in response to powdery mildew infection.
Dingzhong Tang (2006)
Des canaux Ioniques de la membrane plasmique lors de la mort cellulaire programmée induite par l’ozone chez A. thaliana
Q. Tran (2011)
Ectopic Expression in Arabidopsis thaliana of an NB-ARC Encoding Putative Disease Resistance Gene from Wild Chinese Vitis pseudoreticulata Enhances Resistance to Phytopathogenic Fungi and Bacteria
Zhifeng Wen (2015)See more