Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Differential Nitric Oxide (NO) Production By Macrophages From Mice And Guinea Pigs Infected With Virulent And Avirulent Legionella Pneumophila Serogroup 1
P. Rajagopalan-Levasseur, D. Lecointe, G. Bertrand, M. Fay, M. A. Gougerot-Pocidalo
Published 1996 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
l‐arginine‐dependent reactive nitrogen intermediates have been identified as macrophage cytotoxic effector molecules against intracellular pathogens. To determine its role, ex vivo production of NO by peritoneal macrophages of C3H/HeN mice and Dunkin–Hartley guinea pigs infected intraperitoneally with a virulent and isogenic avirulent Legionella pneumophila serogroup 1 strain was compared with bacterial clearance from the lungs. While the virulent strain was cleared from mice lungs, the guinea pigs died within 96 h. In vivo infection with both strains resulted in the production of NO by mouse peritoneal macrophages ex vivo. In contrast, guinea pig macrophages did not produce detectable NO. In addition, infection by the avirulent strain led to the production of significantly more NO by mouse macrophages than the virulent parent strain, irrespective of stimulation with lipopolysaccharide (LPS) and/or interferon‐gamma (IFN‐γ). These results suggest that resistance to Leg. pneumophila infection may depend on the production of NO by host macrophages.
This paper references
Interactions of virulent and avirulent Legionella pneumophila with human polymorphonuclear leukocytes.
J. Summersgill (1988)
Nitric oxide-mediated cytostatic activity on Trypanosoma brucei gambiense and Trypanosoma brucei brucei.
P. Vincendeau (1992)
Microbiostatic effect of murine-activated macrophages for Toxoplasma gondii. Role for synthesis of inorganic nitrogen oxides from L-arginine.
L. Adams (1990)
Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity
C. Nathan (1983)
Release of nitric oxide during the T cell-independent pathway of macrophage activation. Its role in resistance to Listeria monocytogenes.
K. Beckerman (1993)
Metabolic fate of L-arginine in relation to microbiostatic capability of murine macrophages.
D. Granger (1990)
Regulation of transferrin receptor expression and ferritin content in human mononuclear phagocytes. Coordinate upregulation by iron transferrin and downregulation by interferon gamma.
T. Byrd (1993)
Direct activation of human monocyte-derived macrophages by a bacterial glycoprotein extract inhibits the intracellular multiplication of virulent Legionella pneumophila serogroup 1.
P. Rajagopalan (1987)
Synthesis of nitric oxide from a terminal guanidino nitrogen atom of L-arginine: A molecular mechanism regulating cellular proliferation that targets intracellular iron
J. Hibbs (1990)
Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells
D. Stuehr (1989)
Macrophage cytostatic effect on Trypanosoma musculi involves an L-arginine-dependent mechanism.
P. Vincendeau (1991)
Killing of virulent Mycobacterium tuberculosis by reactive nitrogen intermediates produced by activated murine macrophages
J. Chan (1992)
Interferon gamma-activated human monocytes downregulate transferrin receptors and inhibit the intracellular multiplication of Legionella pneumophila by limiting the availability of iron.
T. Byrd (1989)
Cytokine- and Pneumocystis carinii- induced L-arginine oxidation by murine and human pulmonary alveolar macrophages.
M. Sherman (1991)
L . pneumophila inhibits acidification of its phagosome in human monocytes
MA Horwitz (1984)
Killing of Legionella pneumophilaby nitric oxide in -interferonactivated macrophages
JT Summersgill (1992)
FR.L. pneumophilainhibits acidification of its phagosome in human monocytes
MA Horwitz (1984)
Tumor Necrosis Factor and Granulocyte Macrophage‐Colony Stimulating Factor Stimulate Human Macrophages to Restrict Growth of Virulent Mycobacterium avium and to Kill Avirulent M. avium: Killing Effector Mechanism Depends on the Generation of Reactive Nitrogen Intermediates
M. Denis (1991)
The arginine-dependent cytolytic mechanism plays a role in destruction of Naegleria fowleri amoebae by activated macrophages.
K. Fischer-Stenger (1992)
Isolation, purification and partial analysis of the lipopolysaccharide antigenic determinant recognized by a monoclonal antibody to Legionella pneumophila serogroup 1.
F. Petitjean (1990)
Killing of Plasmodium falciparum in vitro by nitric oxide derivatives.
K. Rockett (1991)
Gamma interferon-induced nitric oxide production reduces Chlamydia trachomatis infectivity in McCoy cells.
J. Mayer (1993)
Neutralization of gamma interferon and tumor necrosis factor alpha blocks in vivo synthesis of nitrogen oxides from L-arginine and protection against Francisella tularensis infection in Mycobacterium bovis BCG-treated mice.
S. J. Green (1993)
Monoclonal antibody reactivity as a virulence marker for Legionella pneumophila serogroup 1 strains.
E. Dournon (1988)
Susceptibility of Legionella pneumophila to oxygen-dependent microbicidal systems.
R. Locksley (1982)
Macrophage cytotoxicity against schistosomula of Schistosoma mansoni involves arginine-dependent production of reactive nitrogen intermediates.
S. James (1989)
Role of transferrin, transferrin receptors, and iron in macrophage listericidal activity
C. Alford (1991)
Biosynthesis of nitric oxide activates iron regulatory factor in macrophages.
J. C. Drapier (1993)
Inactivation of ribonucleotide reductase by nitric oxide.
M. Lepoivre (1991)
Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes
M. Horwitz (1983)
Legionella pneumophila inhibits acidification of its phagosome in human monocytes
M. Horwitz (1984)
Leishmania major amistogotes initiate theL - arginine - dependent killing mechnisms in IFN - - stimulated macrophages by induction of TNF -
SJ Green (1990)
Role of inorganic nitrogen oxides and tumor necrosis factor alpha in killing Leishmania donovani amastigotes in gamma interferon-lipopolysaccharide-activated macrophages from Lshs and Lshr congenic mouse strains.
T. Roach (1991)
Interferon‐γ and tumor necrosis factor induce the L‐arginine‐dependent cytotoxic effector mechanism in murine macrophages*
J. C. Drapier (1988)
L-arginine-dependent reactive nitrogen intermediates and the antimicrobial effect of activated human mononuclear phagocytes.
H. Murray (1992)
Inhibition of Legionella pneumophilagrowth by gamma interferon in permissive A/J mouse macrophages: role of reactive oxygen species, nitric oxide, tryptophan, and iron (III)
SJ Gebran (1994)
Activated mouse macrophages kill Entamoeba histolytica trophozoites by releasing reactive nitrogen intermediates.
M. Denis (1992)
Macrophage cytotoxicity : role of L - arginine deiminase activity and imino nitrogen oxidation to nitrite
JB Hibbs (1987)
L-arginine-dependent macrophage effector functions inhibit metabolic activity of Mycobacterium leprae.
L. Adams (1991)
Serogroup specificity of Legionella pneumophila is related to lipopolysaccharide characteristics.
C. Ciesielski (1986)
Differences in the respiratory burst of human polymorphonuclear leukocytes induced by virulent and avirulent Legionella pneumophila serogroup 1.
P. Rajagopalan-Levasseur (1992)
This paper is referenced by
Type I and II IFNs modify the proteome of bacterial vacuoles to restrict infections via IRG1
J. Naujoks (2015)
Does Inhibition of Tumor Necrosis Factor Alpha Affect Chlamydial Genital Tract Infection in Mice and Guinea Pigs?
T. Darville (2000)
Production and Characterization of Guinea Pig Recombinant Gamma Interferon and Its Effect on Macrophage Activation
A. Jeevan (2006)
A quantitative microbial risk assessment model for Legionnaires' disease: animal model selection and dose-response modeling.
T. Armstrong (2007)
Species-Specific Transcriptional Regulation of Genes Involved in Nitric Oxide Production and Arginine Metabolism in Macrophages
R. Young (2018)
Nitric Oxide Inhibits Coxiella burnetii Replication and Parasitophorous Vacuole Maturation
D. Howe (2002)
Innate sensing and cell-autonomous resistance pathways in Legionella pneumophila infection.
J. Naujoks (2018)
Induction of iNOS in human monocytes infected with different Legionella species.
B. Neumeister (2001)
Antimicrobial Properties of Nitric Oxide
M. Degroote (2002)
Biologic Activity of Guinea Pig IFN-γ In Vitro
H. Schaefer (2007)
Effect of cyclosporin A on immunological response in lungs of guinea pigs infected with Trichinella spiralis.
J. Dzik (2002)