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ZntR Is A Zn(II)‐responsive MerR‐like Transcriptional Regulator Of ZntA In Escherichia Coli

K. R. Brocklehurst, J. Hobman, B. Lawley, L. Blank, S. J. Marshall, N. Brown, A. Morby
Published 1999 · Biology, Medicine

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We have identified the promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance (mer ) operons, including a long spacer region containing an inverted repeat sequence. The gene encoding the transcriptional regulator of PzntA, designated zntR, has been identified from genome sequence data, by expression of the gene product and by insertional inactivation/complementation. The ZntR product is a member of the MerR family of transcriptional regulators and appears to act as a hypersensitive transcriptional switch. A hybrid MerR/ZntR protein has been constructed and indicates that the C‐terminal region of ZntR recognizes Zn(II).
This paper references
10.1093/NAR/18.17.5157
Site-specific insertion and deletion mutants in the mer promoter-operator region of Tn501; the nineteen base-pair spacer is essential for normal induction of the promoter by MerR.
J. Parkhill (1990)
10.1002/JCB.240460204
Bacterial resistances to mercury and copper
Andrew P. Morby (1991)
10.1016/0378-1119(91)90365-I
New pUC-derived cloning vectors with different selectable markers and DNA replication origins.
Vieira Jeffrey (1991)
Bacterial mercuryresistance genes
J. L. Hobman (1997)
10.1006/ABIO.1993.1184
A Short Course in Bacterial Genetics. A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. By Jeffrey H. Miller. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1992.
S. Garges (1993)
10.1099/00221287-132-2-465
Transcriptional regulation of the mercury-resistance genes of transposon Tn501.
P. Lund (1986)
Construction and characterisation
J. Parkhill (1993)
Analysis of Escherichia coli promoter sequences
C. B. Harley (1987)
10.1093/nar/22.13.2576
Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes
H. Sofia (1994)
Influence of the sequence - dependent flexure of DNA on transcription in Escherichia coli
C. M. Collis (1989)
10.1101/SQB.1974.039.01.090
Studies on the Epstein-Barr Virus genome and the EBV-determined nuclear antigen in human malignant disease.
G. Klein (1975)
10.1111/j.1365-2958.1997.mmi518.x
Zinc(II) tolerance in Escherichia coli K‐12: evidence that the zntA gene (o732) encodes a cation transport ATPase
S. J. Beard (1997)
10.1093/NAR/15.5.2343
Analysis of E. coli promoter sequences.
C. Harley (1987)
The MerR homodimer binds the mer operator–promoter dyad asymmetrically and causes helical distortion in mer operator
I. W. Lee (1991)
10.1074/jbc.273.33.21393
Identification of a Novel Transcription Regulator from Proteus mirabilis, PMTR, Revealed a Possible Role of YJAI Protein in Balancing Zinc in Escherichia coli *
M. Noll (1998)
Site specific insertion and deletion mutants in the mer operator-promoter region of Tn501 ; the 19 base-pair spacer is essential for normal induction of the promoter by MerR
J. Parkhill (1990)
Zinc (II) tolerance in Escheri
M.N (1997)
Copper and lead resistance in Alcaligenes eutrophus CH34
B Borremans (1997)
Cyanobacterial metal
J. S. Turner (1995)
10.1093/nar/12.1Part1.387
A comprehensive set of sequence analysis programs for the VAX
J. Devereux (1984)
10.1038/374370A0
DNA-bend modulation in a repressor-to-activator switching mechanism
A. Ansari (1995)
10.1073/PNAS.86.10.3544
Cadmium resistance from Staphylococcus aureus plasmid pI258 cadA gene results from a cadmium-efflux ATPase.
G. Nucifora (1989)
Metal-responsive gene regulation and the zinc metalloregulatory model.
D. A. Suhy (1996)
Preliminary characterisation of zntA, a gene which encodes a Zn (II)/Cd (II) -export protein in Escherichia coli
D K Blencowe (1997)
10.1016/0378-1119(89)90359-4
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
R. M. Horton (1989)
10.1002/JCB.240460102
Growth factors and wound repair
R. A. Clark (1991)
10.1128/JB.177.14.3904-3910.1995
Two highly similar multidrug transporters of Bacillus subtilis whose expression is differentially regulated.
M. Ahmed (1995)
10.1016/0147-619X(92)90002-R
Bacterial resistances to inorganic mercury salts and organomercurials.
T. Misra (1992)
The ZnuABC high-affinity
S. I. Patzer (1998)
10.1073/PNAS.94.26.14326
The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase.
C. Rensing (1997)
10.1021/BI00441A002
Mutagenesis of the cysteines in the metalloregulatory protein MerR indicates that a metal-bridged dimer activates transcription.
L. Shewchuk (1989)
10.1007/978-94-009-5736-7
Metals in Biochemistry
P. Harrison (1980)
10.1093/NAR/17.22.9447
Influence of the sequence-dependent flexure of DNA on transcription in E. coli.
C. M. Collis (1989)
10.1046/J.1365-2958.1998.00883.X
The ZnuABC high‐affinity zinc uptake system and its regulator Zur in Escherichia coli
S. Patzer (1998)
Construction and properties of a family of pACYC 184 derived cloning vectors compatible with pBR 322 and its derivatives
B. Bartolome (1991)
10.1021/BI00472A001
DNA distortion accompanies transcriptional activation by the metal-responsive gene-regulatory protein MerR.
B. Frantz (1990)
10.1093/NAR/19.16.4479
Molecular characterization of the soxRS genes of Escherichia coli: two genes control a superoxide stress regulon.
C. F. Amábile-Cuevas (1991)
10.1099/00221287-143-4-1191
Copper-inducible transcriptional regulation at two promoters in the Escherichia coli copper resistance determinant pco.
D. Rouch (1997)
per and lead resistance in Alcaligenes eutrophus CH 34
B. Borremans (1997)
10.1002/j.1460-2075.1993.tb05987.x
Autogenous transcriptional activation of a thiostrepton‐induced gene in Streptomyces lividans.
D. Holmes (1993)
10.1073/PNAS.87.10.3846
Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex.
D. M. Ralston (1990)
[Bacterial resistances].
M. G. García Sánchez (1999)
bacterial mercury-resistance genes.
J. Hobman (1997)
10.1110/PS.8.3.614
Functional insights from structural predictions: Analysis of the Escherichia coli genome
L. Rychlewski (1999)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1016/0378-1119(91)90365-i
New pUC-derived cloning vectors with different selectable markers and DNA replication origins.
J. Vieira (1991)
10.1128/JB.169.11.5101-5112.1987
Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.
P. Rogowsky (1987)
10.1016/0092-8674(89)90990-2
The MerR heavy metal receptor mediates positive activation in a topologically novel transcription complex
T. O’Halloran (1989)
10.1002/j.1460-2075.1993.tb05673.x
Construction and characterization of a mercury‐independent MerR activator (MerRAC): transcriptional activation in the absence of Hg(II) is accompanied by DNA distortion.
J. Parkhill (1993)
10.1128/JB.177.15.4437-4441.1995
CadC, the transcriptional regulatory protein of the cadmium resistance system of Staphylococcus aureus plasmid pI258.
G. Endo (1995)
10.1016/0378-1119(94)90847-8
A merR homologue at 74 minutes on the Escherichia coli genome.
G. Christie (1994)
mercury and copper
G. E. Christie (1994)
Analysis of the Escherichia coli genome . 5 . DNA - sequence of the region from 76 . 0 - minutes to 81 . 5 - minutes
H. J. Sofia (1994)
Cyanobacterial metal - lothioneins – biochemistry and molecular genetics
N. J. Robinson (1995)
10.1128/JB.174.10.3097-3101.1992
Untwist and shout: a heavy metal-responsive transcriptional regulator.
A. Summers (1992)
Zinc deficiency in elderly patients.
A. Prasad (1993)
10.1038/355087A0
Allosteric underwinding of DNA is a critical step in positive control of transcription by Hg-MerR
A. Ansari (1992)
10.1021/BI00493A011
Activator-dependent preinduction binding of sigma-70 RNA polymerase at the metal-regulated mer promoter.
A. Heltzel (1990)
10.1016/0378-1119(91)90541-I
Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives.
B. Bartolomé (1991)
Identification of a putative metal binding site in a new family of metalloregulatory proteins.
W. Shi (1994)
liminary characterisation of zntA , a gene which encodes a Zn ( II ) / Cd ( II ) - export protein in Escherichia coli
D. K. Blencowe (1997)
10.1016/s0021-9258(18)46956-6
A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates.
M. Ahmed (1994)



This paper is referenced by
10.1074/jbc.R114.588814
The Mismetallation of Enzymes during Oxidative Stress*
J. Imlay (2014)
10.7939/R30K26Q1V
Cellular role of the inhibitor-chaperone CpxP in Escherichia coli
J. Wong (2015)
10.1128/JB.00173-07
A galvanizing story--protein stability and zinc homeostasis.
P. Chivers (2007)
10.3390/s150408981
Determination of Zinc, Cadmium and Lead Bioavailability in Contaminated Soils at the Single-Cell Level by a Combination of Whole-Cell Biosensors and Flow Cytometry
Quentin Hurdebise (2015)
10.3389/fmicb.2015.00648
Transcription factor-based biosensors enlightened by the analyte
R. Fernández-López (2015)
Zinc on the move : insights towards understanding zinc homeostasis in the open ocean cyanobacterium Synechococcus sp. WH8102
Amira Z. Ksibe (2017)
Escherichia coli-Dependent Genes in 54 ς Themes of Metabolic Context and Possible Physiological
L. Reitzer (2001)
Genomic environment of cueR and copA genes for prodigiosin biosynthesis by Serratia marcescens SB08
Chidambaram Kulandaisamy Venil (2009)
10.1128/AEM.67.4.1437-1444.2001
Chromosomal Locus for Cadmium Resistance in Pseudomonas putida Consisting of a Cadmium-Transporting ATPase and a MerR Family Response Regulator
S. Lee (2001)
10.1007/7171_2006_083
Zinc, Cadmium, and Lead Resistance and Homeostasis
C. Rensing (2007)
10.1099/00221287-148-12-3801
Regulation of yodA encoding a novel cadmium-induced protein in Escherichia coli.
A. Puskárová (2002)
Zinc Ion Stress and Photo-radical Redox Stress Mechanisms of ZnO nanoparticle toxicity to Escherichia coli K 12 enhanced by the nano-bio interface
Jonathan McQuillan (2015)
10.1007/s00284-012-0117-4
Transcriptional and Functional Studies of a Cd(II)/Pb(II)-Responsive Transcriptional Regulator(CmtR) from Acidithiobacillus ferrooxidans ATCC 23270
C. Zheng (2012)
10.1128/JB.00271-08
Glutathione and transition-metal homeostasis in Escherichia coli.
Kerstin Helbig (2008)
Members of the Fur protein family regulate iron and zinc transport in E. coli and characteristics of the Fur-regulated fhuF protein.
K. Hantke (2002)
10.1046/j.1365-2958.2001.02264.x
CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA
J. V. Stoyanov (2001)
Escherichia coli Cadmium Toxicity in Global Gene Expression Responses to
A. Wang (2005)
Marko Virta KT 2440 Pseudomonas putida Resistance in Mediation of Divalent Heavy Metal Interplay of Different Transporters in the
Anu Leedjärv (2007)
Regulator ATPase and a MerR Family Response Consisting of a Cadmium-Transporting Pseudomonas putida Resistance in Chromosomal Locus for Cadmium
Seon-Woo Lee (2001)
10.1101/2020.02.11.941120
Tough Hydrogel-Based Biocontainment of Engineered Organisms for Continuous, Self-Powered Sensing and Computation
Tzu-Chieh Tang (2020)
10.1021/es3024918
Online detection of metals in environmental samples: comparing two concepts of bioluminescent bacterial biosensors.
S. Jouanneau (2012)
10.1099/MIC.0.26225-0
Two MerR homologues that affect copper induction of the Bacillus subtilis copZA operon.
A. Gaballa (2003)
10.1016/S0378-1119(02)01086-7
Characterization of two regulatory genes of the mercury resistance determinants from TnMERI1 by luciferase-based examination.
C. Huang (2002)
10.1016/B978-0-12-381043-4.00005-2
Metal sensing in Salmonella: implications for pathogenesis.
D. Osman (2011)
10.1016/S0147-6513(03)00057-5
Issues underlying use of biosensors to measure metal bioavailability.
C. Rensing (2003)
10.1080/00032719.2020.1755867
Inorganic Cadmium Detection Using a Fluorescent Whole-Cell Bacterial Bioreporter
E. Elcin (2020)
10.1128/JB.01901-06
Zinc-responsive regulation of alternative ribosomal protein genes in Streptomyces coelicolor involves zur and sigmaR.
Gillian A Owen (2007)
10.1016/S0168-6445(03)00041-X
Zn(II) metabolism in prokaryotes.
Dayle K Blencowe (2003)
10.1021/BI050094V
Structural and functional characterization of Mycobacterium tuberculosis CmtR, a PbII/CdII-sensing SmtB/ArsR metalloregulatory repressor.
Yun Wang (2005)
10.1186/1471-2180-6-49
Comparative genomics of regulation of heavy metal resistance in Eubacteria
E. Permina (2005)
Développement d'un biocapteur bactérien pour la détection de métaux lourds
Karl Bernhard Riether (2001)
Mathematical modeling of metal ion homeostasis and signaling systems
Cui ()
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