Online citations, reference lists, and bibliographies.
← Back to Search

The Interaction Domains Of The DnaA And DnaB Replication Proteins Of Escherichia Coli

H. Seitz, C. Weigel, W. Messer
Published 2000 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The initiation of chromosome replication in Escherichia coli requires the recruitment of the replicative helicase DnaB from the DnaBC complex to the unwound region within the replication origin oriC, supported by the oriC‐bound initiator protein DnaA. We defined physical contacts between DnaA and DnaB that involve residues 24–86 and 130–148 of DnaA and residues 154–210 and 1–156 of DnaB respectively. We propose that contacts between DnaA and DnaB occur via two interaction sites on each of the proteins. Interaction domain 24–86 of DnaA overlaps with its N‐terminal homo‐oligomerization domain (residues 1–86). Interaction domain 154–210 of DnaB overlaps or is contiguous with the domains known to interact with plasmid initiator proteins. Loading of the DnaBC helicase in vivo can only be performed by DnaA derivatives containing (in addition to residues 24–86 and the DNA‐binding domain 4) a structurally intact domain 3. Nucleotide binding by domain 3 is, however, not required. The parts of DnaA required for replication of pSC101 were clearly different from those used for helicase loading. Domains 1 and 4 of DnaA, but not domain 3, were found to be involved in the maintenance of plasmid pSC101.
This paper references
The DnaC protein
E. Lanka (1983)
10.1073/PNAS.94.4.1154
Cryptic single-stranded-DNA binding activities of the phage lambda P and Escherichia coli DnaC replication initiation proteins facilitate the transfer of E. coli DnaB helicase onto DNA.
B. Learn (1997)
10.1073/PNAS.85.23.8973
Promoters largely determine the efficiency of repressor action.
M. Lanzer (1988)
10.1093/NAR/11.4.987
The dnaC protein of Escherichia coli. Purification, physical properties and interaction with dnaB protein.
E. Lanka (1983)
10.1073/PNAS.76.9.4308
A general priming system employing only dnaB protein and primase for DNA replication.
K. Arai (1979)
10.1007/s004380050294
The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli
Y. Kawasaki (1996)
10.1016/S0300-9084(99)00215-1
Functional domains of DnaA proteins.
W. Messer (1999)
10.1128/9781555817480_ch7
DNA replication.
M. Gefter (1975)
10.1074/jbc.271.29.17035
Ordered and Sequential Binding of DnaA Protein to oriC, the Chromosomal Origin of Escherichia coli*
C. Margulies (1996)
DnaA protein binding
W. Messer (1987)
10.1111/j.1365-2958.1995.mmi_17050825.x
The speed of the Escherichia coli fork in vivo depends on the DnaB:DnaC ratio
K. Skarstad (1995)
10.1002/j.1460-2075.1995.tb07202.x
The DNA binding domain of the initiator protein DnaA.
A. Roth (1995)
10.1073/PNAS.83.12.4423
Host participation in plasmid maintenance: dependence upon dnaA of replicons derived from P1 and F.
E. B. Hansen (1986)
Replicatively active complexes
A. Kornberg (1993)
10.1093/NAR/20.8.1997
DnaC protein contains a modified ATP-binding motif and belongs to a novel family of ATPases including also DnaA.
E. Koonin (1992)
10.1093/emboj/16.21.6574
DnaA protein binding to individual DnaA boxes in the Escherichia coli replication origin, oriC
C. Weigel (1997)
10.1016/0014-5793(96)00558-3
A dnaA box can functionally substitute for the priming signals in the oriV of the broad host‐range plasmid RSF 1010
Y. Taguchi (1996)
10.1074/jbc.272.32.20173
Role of TrfA and DnaA Proteins in Origin Opening during Initiation of DNA Replication of the Broad Host Range Plasmid RK2*
I. Konieczny (1997)
Initiation of chromosome replication
W. Messer (1996)
10.1002/(SICI)1097-0134(199705)28:1<1::AID-PROT1>3.0.CO;2-K
Prediction of the structure of the replication initiator protein DnaA
Sigrid Schaper and (1997)
10.1046/j.1365-2958.1999.01568.x
The N‐terminus promotes oligomerization of the Escherichia coli initiator protein DnaA
C. Weigel (1999)
10.1073/PNAS.96.1.73
Mechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101.
H. J. Datta (1999)
10.1006/JMBI.1993.1481
Replicatively active complexes of DnaA protein and the Escherichia coli chromosomal origin observed in the electron microscope.
Elliott Crooke (1993)
Replication fork propagation
K. J. Marians (1996)
Replication of plasmid pSC 101 in Escherichia coli K 12 : requirement for dnaA function
K. Hasunuma (1977)
Interaction of the initiator
S. Schaper (1995)
Interaktion des E. coli Initiatorproteins DnaA mit der replikativen Helikase DnaB
H. Seitz (2000)
From footprint to toeprint : a close - up of the DnaA box , the binding site for the initiator protein DnaA of Escherichia coli
C. Speck (1997)
DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli.
J. Marszalek (1994)
Escherichia coli DnaA protein . The N - terminal domain and loading of DnaB helicase at the E . coli chromosomal origin
M. D. Sutton (1998)
A general priming system employing only DnaB and primase for DNA replication
K. Arai (1979)
10.1073/PNAS.86.7.2172
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.
C. T. Chung (1989)
A dnaA box can functionally
T Komano (1996)
The dnaA protein complex with the E . coli chromosomal origin ( oriC ) and other sites
R. S. Fuller (1984)
10.1006/JMBI.1997.1425
The Escherichia coli dnaA gene: four functional domains.
M. Sutton (1997)
10.1073/PNAS.93.11.5522
The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB.
P. V. Ratnakar (1996)
10.1038/250194A0
Chemical and biological evolution of a nucleotide-binding protein
M. Rossmann (1974)
10.1101/GAD.5.8.1453
Cooperativity at a distance promoted by the combined action of two replication initiator proteins and a DNA bending protein at the replication origin of pSC101.
T. T. Stenzel (1991)
10.1016/0092-8674(84)90284-8
The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites
R. Fuller (1984)
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P 15 A cryptic miniplasmid
S. N. Cohen (1978)
10.1128/JB.134.3.1141-1156.1978
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
A. C. Chang (1978)
10.1093/NAR/25.16.3242
From footprint to toeprint: a close-up of the DnaA box, the binding site for the bacterial initiator protein DnaA.
C. Speck (1997)
10.1074/JBC.270.29.17622
Interaction of the Initiator Protein DnaA of Escherichia coli with Its DNA Target (*)
S. Schaper (1995)
Experiments in molecular genetics
J. Miller (1972)
10.1074/jbc.271.31.18535
Domains of DnaA Protein Involved in Interaction with DnaB Protein, and in Unwinding the Escherichia coli Chromosomal Origin*
J. Marszalek (1996)
Cryptic single-stranded-DNA binding activities of the phage l P and Escherichia coli DnaC replication initiation proteins facilitate the transfer of E. coli DnaB helicase onto DNA (phage l DNA replicationyE. coliDNA replicationyregulation of DNA helicase action)
S. Oo-Jongum (1997)
10.1093/emboj/17.17.5192
Mechanistic studies of initiator–initiator interaction and replication initiation
Y. Lu (1998)
10.1021/BI990048T
Mechanism of DnaB helicase of Escherichia coli: structural domains involved in ATP hydrolysis, DNA binding, and oligomerization.
E. Biswas (1999)
10.1016/0092-8674(87)90357-6
DnaA protein binding to the plasmid origin region can substitute for primosome assembly during replication of pBR322 in vitro
W. Seufert (1987)
10.1006/JMBI.1997.1404
Complexes at the replication origin of Bacillus subtilis with homologous and heterologous DnaA protein.
M. Krause (1997)
Coop - erativity at a distance promoted by the combined action of two replication initiator proteins and a DNA bending protein at the replication origin of pSC 101
T. T. Stenzel (1991)
10.1002/0471684228.egp07910
Molecular cloning.
D. Hamer (1977)
10.1006/JSBI.1995.1016
A structural model for the Escherichia coli DnaB helicase based on electron microscopy data.
M. C. San Martín (1995)
DnaA of Escherichia coli
G. Kellenberger-Gujer (1978)
10.1007/s004380050679
Unique organization of the dnaA region from Prochlorococcus marinus CCMP1375, a marine cyanobacterium
S. Richter (1998)
Escherichia coli dnaB protein. Affinity chromatography on immobilized nucleotides.
E. Lanka (1978)



This paper is referenced by
10.1074/jbc.M409695200
The Box VII Motif of Escherichia coli DnaA Protein Is Required for DnaA Oligomerization at the E. coli Replication Origin*
Magdalena M. Felczak (2004)
10.1007/978-1-4614-6436-5_142-1
DnaA, DnaB, and DnaC
J. Kaguni (2014)
10.1016/j.cell.2008.09.058
Structural Synergy and Molecular Crosstalk between Bacterial Helicase Loaders and Replication Initiators
M. Mott (2008)
10.1093/embo-reports/kve225
A hybrid bacterial replication origin
H. Seitz (2001)
10.1016/S0300-9084(00)01216-5
Bacterial replication initiator DnaA. Rules for DnaA binding and roles of DnaA in origin unwinding and helicase loading.
W. Messer (2001)
10.1016/B978-0-12-809633-8.12304-0
Regulation of Replication Origin Firing
Alan C. Leonard (2016)
Streptomyces and Escherichia coli, model organisms for the analysis of the initiation of bacterial chromosome replication.
W. Messer (2002)
10.1016/j.plasmid.2009.06.003
DnaA structure, function, and dynamics in the initiation at the chromosomal origin.
S. Ozaki (2009)
The double mechanism of incompatibility between λ plasmids and Escherichia coli dnaA ( ts ) host cells
M. Glinkowska ()
10.1111/j.1574-6976.2011.00300.x
AT-rich region and repeated sequences - the essential elements of replication origins of bacterial replicons.
M. Rajewska (2012)
10.1093/emboj/20.12.3262
A broad host range replicon with different requirements for replication initiation in three bacterial species
R. Caspi (2001)
10.4161/gmic.1.5.13115
Structural insight into Helicobacter pylori DNA replication initiation
L. Terradot (2010)
10.1111/mmi.14423
The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication
Malgorzata Nowaczyk-Cieszewska (2019)
10.1128/JB.185.22.6633-6639.2003
Purification and characterization of the PcrA helicase of Bacillus anthracis.
Asma Naqvi (2003)
10.1074/jbc.M111.233403
DnaA Protein DNA-binding Domain Binds to Hda Protein to Promote Inter-AAA+ Domain Interaction Involved in Regulatory Inactivation of DnaA*
Kenji Keyamura (2011)
Bell and Kaguni Helicase Loading 1 Helicase Loading at Chromosomal Origins of Replication
S. Bell (2014)
10.1111/j.1365-2958.2012.08129.x
Evidence for roles of the Escherichia coli Hda protein beyond regulatory inactivation of DnaA
Jamie C Baxter (2012)
10.1111/j.1365-2958.2010.07074.x
Novel essential residues of Hda for interaction with DnaA in the regulatory inactivation of DnaA: unique roles for Hda AAA+ Box VI and VII motifs
K. Nakamura (2010)
10.1016/j.plasmid.2014.10.003
Two replication initiators - one mechanism for replication origin opening?
Elzbieta Zabrocka (2014)
10.1016/J.BIOCHI.2005.08.009
Escherichia coli DnaA protein: specific biochemical defects of mutant DnaAs reduce initiation frequency to suppress a temperature-sensitive dnaX mutation.
J. R. Walker (2006)
10.1074/jbc.M115.662601
The Arg Fingers of Key DnaA Protomers Are Oriented Inward within the Replication Origin oriC and Stimulate DnaA Subcomplexes in the Initiation Complex*
Yasunori Noguchi (2015)
10.1093/emboj/cdg003
Stable co‐existence of separate replicons in Escherichia coli is dependent on once‐per‐cell‐cycle initiation
K. Skarstad (2003)
10.1074/jbc.M701841200
Structure and Function of DnaA N-terminal Domains
Y. Abe (2007)
10.1111/j.1365-2958.2007.05843.x
Structural similarity between the DnaA‐binding proteins HobA (HP1230) from Helicobacter pylori and DiaA from Escherichia coli
G. Natrajan (2007)
10.1016/S0300-9084(00)01230-X
Defective initiation in an Escherichia coli dnaA(Cs,Sx) mutant.
E. Boye (2001)
10.1128/JB.183.6.2148-2150.2001
DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.
D. Ishigo-Oka (2001)
10.1128/9781555817992.CH7
Chromosome Replication and Segregation
K. Lemon (2002)
Structure et dynamique de protéines intrinsèquement désordonnées : Caractérisation par une approche combinant dynamique moléculaire avancée et SAXS
Maud Chan Yao Chong (2019)
10.1099/MIC.0.28693-0
The replicative polymerases PolC and DnaE are required for theta replication of the Bacillus subtilis plasmid pBS72.
M. Titok (2006)
Bacillus anthracis PcrA Helicase of Purification and Characterization of the
Asma Naqvi (2003)
10.1016/j.febslet.2010.11.018
Helicobacter pylori chromosomal DNA replication: Current status and future perspectives
R. G. Nitharwal (2011)
10.1016/j.cbpa.2011.07.016
Replication initiation at the Escherichia coli chromosomal origin.
J. Kaguni (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar