Online citations, reference lists, and bibliographies.
← Back to Search

Fruit‐specific Expression Of The A. Tumefaciens Isopentenyl Transferase Gene In Tomato: Effects On Fruit Ripening And Defense‐related Gene Expression In Leaves

B. Martineau, C. Houck, R. E. Sheehy, W. Hiatt
Published 1994 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Summary This paper describes the analysis of tomato plants transformed with a chimeric gene consisting of the promoter region of a fruit specifically expressed tomato gene linked to the ipt gene coding sequences from the Ti plasmid of Agrobacterium tumefaciens. The pattern of expression of this chimeric gene was found to be consistent with the expression of the endogenous fruit-specific gene and consequently, plants expressing the chimeric gene were phenotypically normal until fruit maturation and ripening. A dramatically altered fruit phenotype, islands of green pericarp tissue remaining on otherwise deep red ripe fruit, was then evident in many of the transformed plants. Cytokinin levels in transformed plant fruit tissues were 10 to 100-fold higher than in control fruit. In the leaves of a fruit-bearing transformant, despite a lack of detectable ipt mRNA accumulation, approximately fourfold higher than control leaf levels of cytokinin were detected. It is suggested that cytokinin produced in fruit is being transported to the leaves since accumulation in leaves of PR-1 and chitinase mRNAs, which encode defense-related proteins known to be induced by cytokinin, occurred only when the transformant was reproductively active. Effects of elevated cytokinin levels on tomato fruit gene expression and cellular differentiation processes are also described.

This paper is referenced by
Genetic Engineering of Cytokinins and Their Application to Agriculture
Q. Ma (2008)
Fruit and Vegetable Biotechnology
V. Valpuesta (2002)
Down-regulation of DR12, an auxin-response-factor homolog, in the tomato results in a pleiotropic phenotype including dark green and blotchy ripening fruit.
B. Jones (2002)
Leaf Extracts from Cytokinin­ Overproducing Transgenic Plants Kill Sugarbeet Root Maggot (Tetanops myopaeformis) Larvae
A. Smigocki (2003)
Cytokinins in plant senescence: From spray and pray to clone and play
S. Gan (1996)
Water-Deficit Inducible Expression of a Cytokinin Biosynthetic Gene IPT Improves Drought Tolerance in Cotton
S. Kuppu (2013)
Effect of alien ipt gene on hormonal concentrations of plants
R. V. Makarova (1996)
Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry
Attila Molnár (2004)
Genetic Manipulation of Leaf Senescence
Yongfeng Guo (2007)
Response of dihaploid tobacco roots to salt stress
Tihana Marček (2017)
Hormonal Regulation of Tomato Fruit Development: A Molecular Perspective
A. Srivastava (2005)
Genetic engineering of cytokinin metabolism: prospective way to improve agricultural traits of crop plants.
David Zalabák (2013)
Noninvasive Expressions of ipt in Whole Plants or Roots through pOp/LhG4 Indicate a Role of Plant Aerial Parts and Light in Cytokinin Synthesis and Root Inhibition
Jianchun Guo (2008)
Environmental perception avenues: the interaction of cytokinin and environmental response pathways.
Cristiana T. Argueso (2009)
Sense and antisense inactivation of fruit ripening genes in tomato.
A. Hamilton (1995)
Plant promoters for transgene expression
R. Datla (1997)
Consequences of transforming narrow leafed lupin (Lupinus angustifolius [L.]) with an ipt gene under control of a flower-specific promoter
Craig A. Atkins (2011)
Plantes transgeniques aux caracteristiques de senescence modifiees
R. Amasino (1996)
Seed-specific expression of the isopentenyl transferase gene ( ipt) in transgenic tobacco
Q. Ma (1998)
Expression of the ipt Gene with the AGPase S1 Promoter in Tomato Results in Unbranched Roots and Delayed Leaf Senescence
Y. Luo (2005)
Mechanisms of the light-dependent induction of cell death in tobacco plants with delayed senescence.
A. Wingler (2005)
Expression of a wound-inducible cytokinin biosynthesis gene in transgenic tobacco: correlation of root expression with induction of cytokinin effects
A. Smigocki (1995)
Activity of the yeast mevalonate kinase promoter in transgenic tobacco
Sandrine Champenoy (1999)
Development of disease-resistant marker-free tomato by R/RS site-specific recombination
R. S. Khan (2011)
Proteomic analysis of stress-related proteins in transgenic broccoli harboring a gene for cytokinin production during postharvest senescence.
Mao-sen Liu (2011)
Transgenic grapefruit plants expressing the PAPETALA3-IPTgp gene exhibit altered expression of PR genes
Gemma Pasquali (2009)
Cytokinin-regulated transcripts in tobacco cell culture
S. Schäfer (2004)
Cytokinins as regulators of gene expression
T. Schmülling (1997)
Programmed senescence of plant organs
K. A. Hadfield (1997)
Low level expression of prokaryotic tzs gene enhances growth performance of transgenic poplars
Y. Choi (2009)
Increasing tomato fruit quality by enhancing fruit chloroplast function. A double-edged sword?
Maria Florencia Cocaliadis (2014)
Cloning and characterization of promoters from American chestnut capable of directing reporter gene expression in transgenic Arabidopsis plants
Bernadette J. Connors (2002)
See more
Semantic Scholar Logo Some data provided by SemanticScholar