← Back to Search
The Gene Coding For The Hrp Pilus Structural Protein Is Required For Type III Secretion Of Hrp And Avr Proteins In Pseudomonas Syringae Pv. Tomato.
W. Wei, A. Plovanich-Jones, W. Deng, Q. Jin, A. Collmer, H. C. Huang, S. He
Published 2000 · Medicine, Biology
Download PDFAnalyze on Scholarcy
Bacterial surface appendages called pili often are associated with DNA and/or protein transfer between cells. The exact function of pili in the transfer process is not understood and is a matter of considerable debate. The Hrp pilus is assembled by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato (Pst) strain DC3000. In this study, we show that the hrpA gene, which encodes the major subunit of the Hrp pilus, is required for secretion of putative virulence proteins, such as HrpW and AvrPto. In addition, the hrpA gene is required for full expression of genes that encode regulatory, secretion, and effector proteins of the type III secretion system. hrpA-mediated gene regulation apparently is through effect on the mRNA level of two previously characterized regulatory genes, hrpR and hrpS. Ectopic expression of the hrpRS gene operon restored gene expression, but not protein secretion, in the hrpA mutant. Three single amino acid mutations at the HrpA carboxyl terminus were identified that affect the secretion or regulatory function of the HrpA protein. These results define an essential role of the Hrp pilus structural gene in protein secretion and coordinate regulation of the type III secretion system in Pst DC3000.
This paper references
Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto Kinase
Xiaoyan Tang (1996)
Bacterial avirulence genes.
J. Leach (1996)
Pilus Assembly by Agrobacterium T-DNA Transfer Genes
K. J. Fullner (1996)
Escherichia-Pseudomonas shuttle vectors derived from pUC18/19.
H. Schweizer (1991)
LcrG, a secreted protein involved in negative regulation of the low-calcium response in Yersinia pestis.
E. Skryzpek (1993)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Type III machines of Gram-negative pathogens: injecting virulence factors into host cells and more.
D. Anderson (1999)
The role of hrp genes during plant-bacterial interactions.
P. B. Lindgren (1997)
The secretion of the Shigella flexneri Ipa invasins is activated by epithelial cells and controlled by IpaB and IpaD.
R. Ménard (1994)
Pseudomonas syringae pv. syringae harpinPss: A protein that is secreted via the hrp pathway and elicits the hypersensitive response in plants
S. He (1993)
Hrp-controlled interkingdom protein transport: learning from flagellar assembly?
S. He (1997)
The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants.
H. Huang (1992)
Determinants of pathogenicity and avirulence in plant pathogenic bacteria.
A. Collmer (1998)
The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit
C. Grimm (1995)
The complete hrp gene cluster of Pseudomonas syringae pv. syringae 61 includes two blocks of genes required for harpinPss secretion that are arranged colinearly with Yersinia ysc homologs.
H. C. Huang (1995)
Negative regulation of hrp genes in Pseudomonas syringae by HrpV.
G. Preston (1998)
The Yersinia deadly kiss.
G. Cornelis (1998)
A novel EspA‐associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells
S. Knutton (1998)
Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium
C. Ginocchio (1994)
prhJ and hrpG, two new components of the plant signal‐dependent regulatory cascade controlled by PrhA in Ralstonia solanacearum
B. Brito (1999)
Supramolecular structure of the Salmonella typhimurium type III protein secretion system.
T. Kubori (1998)
Modulation of Virulence Factor Expression by Pathogen Target Cell Contact
J. Pettersson (1996)
Biology of plant-microbe interactions
G. Stacey (1996)
Cellular locations of Pseudomonas syringae pv. syringae HrcC and HrcJ proteins, required for harpin secretion via the type III pathway.
W. Deng (1999)
A single promoter sequence recognized by a newly identified alternate sigma factor directs expression of pathogenicity and host range determinants in Pseudomonas syringae.
Y. Xiao (1994)
Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000.
E. Roine (1997)
Type III secretion machines: bacterial devices for protein delivery into host cells.
J. Galán (1999)
The Pseudomonas syringae pv. tomato HrpW protein has domains similar to harpins and pectate lyases and can elicit the plant hypersensitive response and bind to pectate.
A. Charkowski (1998)
The Avr (effector) proteins HrmA (HopPsyA) and AvrPto are secreted in culture from Pseudomonas syringae pathovars via the Hrp (type III) protein secretion system in a temperature- and pH-sensitive manner.
K. van Dijk (1999)
Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato
S. Scofield (1996)
PrhA controls a novel regulatory pathway required for the specific induction of Ralstonia solanacearum hrp genes in the presence of plant cells
M. Marenda (1998)
Reciprocal secretion of proteins by the bacterial type III machines of plant and animal pathogens suggests universal recognition of mRNA targeting signals.
D. Anderson (1999)
The surface‐located YopN protein is involved in calcium signal transduction in Yersinia pseudotuberculosis
Å. Forsberg (1991)
Characterization of the Pseudomonas syringae pv. tomato AvrRpt2 protein: demonstration of secretion and processing during bacterial pathogenesis
M. B. Mudgett (1999)
TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors
M. Iriarte (1998)
LcrG is Required for Efficient Translocation ofYersinia Yop Effector Proteins into Eukaryotic Cells
M. R. Sarker (1998)
The Pseudomonas syringae Hrp regulation and secretion system controls the production and secretion of multiple extracellular proteins.
J. Yuan (1996)
The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and tomato are encoded by an operon containing Yersinia ysc homologs and elicit the hypersensitive response in tomato but not soybean.
G. Preston (1995)
Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants
C. Hueck (1998)
Type III protein secretion systems in plant and animal pathogenic bacteria.
S. He (1998)
Unified nomenclature for broadly conserved hrp genes of phytopathogenic bacteria
A. Bogdanove (1996)
Gene cluster of Pseudomonas syringae pv. "phaseolicola" controls pathogenicity of bean plants and hypersensitivity of nonhost plants.
P. B. Lindgren (1986)
Construction of cloning cartridges for development of expression vectors in gram-negative bacteria.
K. Yen (1991)
Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicola.
L. Rahme (1992)
The predicted protein product of a pathogenicity locus from Pseudomonas syringae pv. phaseolicola is homologous to a highly conserved domain of several procaryotic regulatory proteins.
C. Grimm (1989)
Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes.
Y. Xiao (1994)
Induction of Gene Expression in Escherichia coli After Pilus-Mediated Adherence
J. Zhang (1996)
A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica.
D. Anderson (1997)
Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster.
Y. Xiao (1992)
Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells.
R. Rosqvist (1994)
Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity.
T. V. Huynh (1989)
Evidence that bacterial contact with the plant cell is necessary for the hypersensitive reaction but not the susceptible reaction
R. Stall (1979)
The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpin.
J. Kim (1997)
The conjugation system of F-like plasmids.
N. Willetts (1980)
This paper is referenced by
Positive regulation of the Hrp type III secretion system in Pseudomonas syringae pv. phaseolicola.
Inmaculada Ortiz-Martín (2010)
Characterization of Eukaryotic Translation Initiation Factor 5A-2 (eIF5A-2) in Arabidopsis thaliana: Effects of Wounding and Pathogen Attack
Yulia Gatsukovich (2004)
Components of the Pseudomonas syringae type III secretion system can suppress and may elicit plant innate immunity.
Hye-sook Oh (2010)
Regulation of Type III Secretion System in Pseudomonas syringae.
Yingpeng Xie (2019)
Immunocytochemical localization of HrpA and HrpZ supports a role for the Hrp pilus in the transfer of effector proteins from Pseudomonas syringae pv. tomato across the host plant cell wall.
I. Brown (2001)
Pseudomonas syringae pathogenesis: Regulation of type III secretion and identification of a secreted effector
James R Bretz (2004)
Gram-Negativ Plant Pathogenic Bacteria
J. Boch (2001)
Type III protein secretion mechanism in mammalian and plant pathogens.
S. He (2004)
Identification and Secretion of Effectors From the Pseudomonas syringae Type III Secretion System
Losada Bohannon (2004)
Two components of the rhpPC operon coordinately regulate the type III secretion system and bacterial fitness in Pseudomonas savastanoi pv. phaseolicola
K. Li (2019)
HrpB2 and HrpF from Xanthomonas are type III‐secreted proteins and essential for pathogenicity and recognition by the host plant
O. Rossier (2000)
Genetics of Phytopathogenic Bacteria
A. Burger (2003)
Pseudomonas syringae pv. phaseolicola Mutants Compromised for type III secretion system gene induction.
X. Deng (2009)
A Na+/Ca2+ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence
C. Moretti (2019)
A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants
P. Hauck (2003)
mRNA stability and the secretion signal of HrpA, a pilin secreted by the type III system in Pseudomonas syringae
E. Hienonen (2001)
Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host.
José S. Rufián (2016)
Investigation Of The Interconnected Roles Of Cmal And Hopaa1-1 In The Virulence Of Pseudomonas Syringae Pv.Tomato Dc3000 In Nicotiana Benthamiana
J. Worley (2013)
Getting across—bacterial type III effector proteins on their way to the plant cell
D. Büttner (2002)
Computational prediction of type III secreted proteins from gram-negative bacteria
Y. Yang (2010)
HrpZ(Psph) from the plant pathogen Pseudomonas syringae pv. phaseolicola binds to lipid bilayers and forms an ion-conducting pore in vitro.
J. Lee (2001)
THE MOLECULAR BIOLOGY OF HYPERSENSITIVITY TO PLANT PATHOGENIC BACTERIA
S. Hutcheson (2001)
The Hrp pilus of Pseudomonas syringae elongates from its tip and acts as a conduit for translocation of the effector protein HrpZ
C. Li (2002)
Genomewide identification of Pseudomonas syringae pv. tomato DC3000 promoters controlled by the HrpL alternative sigma factor
D. Fouts (2002)
INVITED REVIEW THE MOLECULAR BIOLOGY OF HYPERSENSITIVITY TO PLANT PATHOGENIC BACTERIA
S. Hutcheson (2001)
Type III protein secretion in Pseudomonas syringae.
Q. Jin (2003)
Plant-Associated Bacteria Involved in Host-Microbe Interactions by Detection of and Response to Signals
Anja Brencic (2005)
The Arabidopsis Thaliana-Pseudomonas Syringae Interaction
F. Katagiri (2002)
A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants.
Sruti DebRoy (2004)
Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
William Truman (2006)
Use of Dominant-negative HrpA Mutants to Dissect Hrp Pilus Assembly and Type III Secretion in Pseudomonas syringae pv. tomato*
Y. Lee (2005)
Pseudomonas savastanoi Two-Component System RhpRS Switches between Virulence and Metabolism by Tuning Phosphorylation State and Sensing Nutritional Conditions
Yingpeng Xie (2019)See more