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Localization Of Acetylcholine Receptor By 125I-labeled Alpha-bungarotoxin Binding At Mouse Motor Endplates.

H. C. Fertuck, M. Salpeter
Published 1974 · Chemistry, Medicine

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Exposed sternomastoid muscles of anaesthetized mice were bathed in (125)I-labeled alpha-bungarotoxin until all neurally evoked muscle contractions were eliminated. The distribution of label was then determined by electron microscope autoradiography. It was found that the label was localized at the top of the junctional folds, i.e., at the postjunctional membrane nearest the axon. Since the alpha-bungarotoxin had fully eliminated the physiological muscle response, these results indicate that the active acetylcholine receptor occupies a limited area of the junctional folds and is not distributed uniformly throughout this membrane. Specialized membrane densities seem to coincide with the labeled regions.
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This paper is referenced by
10.1007/978-1-4684-4190-1_1
Immunology of the Neuromuscular Junction
R. Kelly (1982)
10.1016/S0750-7658(84)80008-8
Jonction neuromusculaire et mécanisme d'action des curares
J. Kienlen (1984)
10.1523/JNEUROSCI.12-09-03535.1992
Structure and chromosomal localization of the mammalian agrin gene
F. Rupp (1992)
10.1159/000057322
The Signaling Pathways Mediated by P2Y Nucleotide Receptors in the Formation and Maintenance of the Skeletal Neuromuscular Junction
K. Tsim (2002)
10.1038/254339A0
Immunoperoxidase staining of α-bungarotoxin binding sites in muscle endplates shows distribution of acetylcholine receptors
MATHEW P. Daniels (1975)
10.1016/0014-4886(76)90171-0
Binding sites for 125I-labeled α-bungarotoxin in normal and denervated mouse muscle
P. Howe (1976)
10.1523/JNEUROSCI.05-05-01111.1985
Intracellular acetylcholine receptors in skeletal muscles of the adult rat
A. Pestronk (1985)
10.1080/13550280590900427
Rabies virus receptors
M. Lafon (2011)
10.1016/j.jneumeth.2010.06.015
Identification of neuromuscular junctions by correlative confocal and transmission electron microscopy
S. Modla (2010)
10.1083/JCB.92.1.207
Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
D. Tank (1982)
10.1083/JCB.68.3.752
Studies of excitable membranes. II. A comparison of specializations at neuromuscular junctions and nonjunctional sarcolemmas of mammalian fast and slow twitch muscle fibers
M. Ellisman (1976)
10.1016/0014-4886(75)90171-5
Neuronal recognition and synaptogenesis
S. Appel (1975)
10.1016/0006-8993(81)91115-X
Fluorescent staining of neuromuscular junctions by using the antibody against acetylcholine receptors of Narke japonica, and double staining with the antibody and erabutoxin b
Y. Ishikawa (1981)
10.2535/OFAJ1936.58.4-6_289
Regional differences in surface anionic sites in the rat thyroid follicle cells shown by cationized ferritin labeling.
F. Nishiyama (1982)
10.1016/S0091-679X(08)60186-7
Chapter 3 Fluorescent Analogs of Toxins
K. Angelides (1988)
10.1002/SYN.890160405
Alpha 1‐antichymotrypsin is strongly immunolocalized at normal human and rat neuromuscular junctions
M. Bilak (1994)
10.1007/BF01870626
The density of cholinergic receptors at the endplate postsynaptic membrane: Ultrastructural studies in two mammalian species
C. Porter (2005)
10.1038/264705A0
Selective stabilisation of developing synapses as a mechanism for the specification of neuronal networks
J. Changeux (1976)
10.1021/BI00582A028
Subunit structure and peptide mapping of junctional and extrajunctional acetylcholine receptors from rat muscle.
N. Nathanson (1979)
10.1002/AR.1092070107
Development and innervation of soleplates in the freely grafted extensor digitorum longus (EDL) muscle in the rat
F. M. Hansen-Smith (1983)
10.1679/AOHC.59.375
Neuromuscular junctions of the posterior cricoarytenoid muscle in the adult rat: a scanning electron microscopical study.
S. Kawakita (1996)
10.1016/S0074-7742(08)60541-3
Ultrastructural correlates of neuromuscular junction development.
M. B. Rheuben (1999)
10.1016/0006-8993(77)90192-5
Cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labelled α-bungarotoxin
T. Lentz (1977)
10.1083/JCB.78.1.274
Acetylcholinesterase in the fast extraocular muscle of the mouse by light and electron microscope autoradiography
M. Salpeter (1978)
10.1007/BF01175564
Development of synaptic ultrastructure at neuromuscular contacts in an amphibian cell culture system
P. Weldon (1979)
Development of an amphibian neuromuscular junction in vivo and in culture.
M. Cohen (1980)
10.1113/jphysiol.1988.sp017257
The nature of the presynaptic effects of (+)‐tubocurarine at the mouse neuromuscular junction.
C. Ferry (1988)
10.1007/s10735-008-9169-y
Secreted frizzled related protein 1 (Sfrp1) and Wnt signaling in innervated and denervated skeletal muscle
Anna Svensson (2008)
10.1016/S0079-6123(08)64645-2
Accumulation of acetylcholine receptors at nerve-muscle contacts in culture.
M. Cohen (1979)
10.1016/0306-4522(80)90182-7
Ultrastructural analysis of kainic acid lesion to cerebellar cortex
R. Herndon (1980)
10.1016/0006-8993(80)90713-1
Development of the postsynaptic membrane in xenopus neuromuscular cultures observed by freeze-fracture and thin-section electron microscopy
H. Peng (1980)
10.1523/JNEUROSCI.23-06-02102.2003
Voltage-Gated Sodium Channels and AnkyrinG Occupy a Different Postsynaptic Domain from Acetylcholine Receptors from an Early Stage of Neuromuscular Junction Maturation in Rats
S. Bailey (2003)
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