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Localization Of Acetylcholine Receptor By 125I-labeled Alpha-bungarotoxin Binding At Mouse Motor Endplates.
H. C. Fertuck, M. Salpeter
Published 1974 · Chemistry, Medicine
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Exposed sternomastoid muscles of anaesthetized mice were bathed in (125)I-labeled alpha-bungarotoxin until all neurally evoked muscle contractions were eliminated. The distribution of label was then determined by electron microscope autoradiography. It was found that the label was localized at the top of the junctional folds, i.e., at the postjunctional membrane nearest the axon. Since the alpha-bungarotoxin had fully eliminated the physiological muscle response, these results indicate that the active acetylcholine receptor occupies a limited area of the junctional folds and is not distributed uniformly throughout this membrane. Specialized membrane densities seem to coincide with the labeled regions.
This paper references
Electron microscopic autoradiography of radioiodine in the thyroid using the extranuclear electrons of I-25.
J. Kayes (1962)
ELECTRON MICROSCOPE RADIOAUTOGRAPHY AS A QUANTITATIVE TOOL IN ENZYME CYTOCHEMISTRY
M. Salpeter (1967)
Acetylcholine Receptors: Number and Distribution at Neuromuscular Junctions in Rat Diaphragm
D. Fambrough (1972)
SIZES OF END PLATE COMPARTMENTS, DENSITIES OF ACETYLCHOLINE RECEPTOR AND OTHER QUANTITATIVE ASPECTS OF NEUROMUSCULAR TRANSMISSION
M. Salpeter (1973)
ISOLATION OF NEUROTOXINS FROM THE VENOM OF BUNGARUS MULTICINCTUS AND THEIR MODES OF NEUROMUSCULAR BLOCKING ACTION.
Chang Cc (1963)
Types and locations of cholinergic receptor-like molecules in muscle fibres.
C. Porter (1973)
Localization of the cholinergic receptor protein in Electrophorus electroplax by high resolution autoradiography
J. Bourgeois (1972)
The binding of acetylcholine to receptors and its removal from the synaptic cleft
B. Katz (1973)
AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE
M. Salpeter (1964)
ACETYLCHOLINESTERASE AT THE MYONEURAL JUNCTION: CYTOCHEMICAL ULTRASTRUCTURE AND SOME BIOCHEMICAL CONSIDERATIONS
R. Friedenberg (1972)
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M. B. Rheuben (1999)
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