Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Photosynthetic Electron Transport From Water To NADP Driven By Photosystem II In Inside-out Chloroplast Vesicles.

P. A. Albertsson, B. Hsu, G. M. Tang, D. Arnon
Published 1983 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
It is now widely held that the light-induced noncyclic (linear) electron transport from water to NADP(+) requires the collaboration in series of the two photosystems that operate in oxygen-evolving cells: photosystem II (PSII) photooxidizes water and transfers electrons to photosystem I (PSI); PSI photoreduces ferredoxin, which in turn reduces NADP(+) (the Z scheme). However, a recently described alternative scheme envisions that PSII drives the noncyclic electron transport from water to ferredoxin and NADP(+) without the collaboration of PSI, whose role is limited to cyclic electron transport [Arnon, D. I., Tsujimoto, H. Y. & Tang, G. M.-S. (1981) Proc. Natl. Acad. Sci. USA 78, 2942-2946]. Reported here are findings at variance with the Z scheme and consistent with the alternative scheme. Thylakoid membrane vesicles were isolated from spinach chloroplasts by the two-phase aqueous polymer partition method. Vesicles, originating mainly from appressed chloroplast membranes that are greatly enriched in PSII, were turned inside-out with respect to the original sidedness of the membrane. With added plastocyanin, ferredoxin, and ferredoxin-NADP(+) reductase, the inside-out vesicles enriched in PSII gave a significant photoreduction of NADP(+) with water as electron donor, under experimental conditions that appear to exclude the participation of PSI.

This paper is referenced by
Photosynthetic electron transport: Emergence of a concept, 1949–59
D. Arnon (2004)
Photoreduction of NADP+ by a chloroplast photosystem II preparation: effect of light intensity
D. Arnon (1989)
Short-circuiting the Z-scheme
J. Barber (1995)
Evidence for Cyclic Electron Flow around Photosystem II in Chlorella pyrenoidosa.
P. Falkowski (1986)
The domain organization of the plant thylakoid membrane
P. A. Albertsson (1990)
Perspective on Daniel I. Arnon's contributions to research, 1960–1994
B. Buchanan (2004)
Inside-out thylakoid vesicles. An important tool for the characterization of the photosynthetic membrane
B. Andersson (1985)
17 – Partitioning: A Comprehensive Bibliography
I. Sutherland (1985)
Aqueous two-phase systems strategies for the recovery and characterization of biological products from plants.
O. Aguilar (2010)
Chapter 1 Energy conversion in higher plants and algae
G. Forti (1987)
Photosystem I Is Indispensable for Photoautotrophic Growth, CO2 Fixation, and H2 Photoproduction inChlamydomonas reinhardtii *
K. Redding (1999)
A model of the primary process in photosynthesis
J. Avery (1984)
Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I
L. Cournac (1997)
Divergent pathways of photosynthetic electron transfer: The autonomous oxygenic and anoxygenic photosystems
D. Arnon (2004)
Protein diffusion in cell membranes: some biological implications.
M. McCloskey (1984)
Reexamining the Validity of the Z-Scheme: Is Photosystem I Required for Oxygenic Photosynthesis in Chlamydomonas?
K. Redding (1998)
Semantic Scholar Logo Some data provided by SemanticScholar