← Back to Search
Phosphatidate-dependent Protein Phosphorylation.
S. Bocckino, P. Wilson, J. Exton
Published 1991 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Phosphatidate-dependent protein phosphorylation was observed in soluble extracts from rat liver, brain, lung, and testis. The phosphorylation was stimulated by free Ca2+ in the range of 360-800 nM. Incubation mixtures containing phosphatidate provided markedly different profiles of protein phosphorylation from those with phosphatidylserine plus 1,2-diolein. Phosphatidate-dependent phosphorylation of a 30-kDa protein in the soluble fraction from heart was also observed. This phosphorylation did not require Ca2+. Soluble fractions from liver, testis, brain, and lung phosphorylated the 30-kDa heart protein in a phosphatidate-dependent Ca(2+)-independent manner. We propose that part of the action of phosphatidate in cells may be mediated by a protein kinase(s).
This paper is referenced by
Activation of phospholipase D by G protein-coupled receptors
D. McCulloch (1999)
Nuclear localization of diacylglycerol kinase ζ in neurons
Y. Hozumi (2003)
Phospholipase D-structure, regulation and function.
J. Exton (2002)
Increased phospholipase D activity in human breast cancer
N. Uchida (2005)
Modulation of cell signalling by ceramides.
A. Gomez-Muñoz (1998)
Activation of phospholipase D by guanosine 5′[γ-thio]triphosphate and AlF−4 in bovine corneal epithelial cells
R. Akhtar (1994)
Phosphatidic Acid Generation through Interleukin 2 (IL-2)-induced -Diacylglycerol Kinase Activation Is an Essential Step in IL-2-mediated Lymphocyte Proliferation (*)
I. Flores (1996)
Subcellular alterations in cardiac phospholipase D activity in chronic diabetes.
S. Williams (1997)
Regulation of phosphatidic acid phosphohydrolase activity during stimulation of human polymorphonuclear leukocytes
A. P. Truett Iii (1992)
Cardiac Phospholipase D2 Localizes to Sarcolemmal Membranes and Is Inhibited by α-Actinin in an ADP-ribosylation Factor-reversible Manner*
J. Park (2000)
Regulation of Acetylcholine-Induced Phosphorylation of PLD1 in Porcine Tracheal Smooth Muscle
Abulkhair M. Mamoon (2004)
Stimulation of protein and DNA synthesis in mouse C2C12 satellite cells: Evidence for phospholipase d‐dependent and ‐independent pathways
K. S. Morrison (1995)
Sphingoid bases and phospholipase D activation.
S. Spiegel (1996)
Gene Cloning and Characterization of CDP-diacylglycerol Synthase from Rat Brain*
S. Saito (1997)
Platelet‐activating factor stimulates phosphatidic acid formation in cultured rat mesangial cells: Roles of phospholipase D, diglyceride kinase, and de novo phospholipid synthesis
M. Kester (1993)
Oxidative Stress, Nutritional Antioxidants, and Testosterone Secretion in Men
Glade Mj (2015)
Regulation of phospholipase D by tyrosine kinases.
V. Natarajan (1996)
Oxidant-Mediated Activation of Phospholipases C and D
V. Natarajan (1997)
Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase.
M. Nanjundan (2003)
Protein kinase C and phospholipase D: intimate interactions in intracellular signaling
K. P. Becker (2005)
Messenger functions of phosphatidic acid.
D. English (1996)
Phorbol ester‐induced priming of superoxide generation by phosphatidic acid‐stimulated neutrophils and granule‐free neutrophil cytoplasts
R. Siddiqui (1995)
Enhancement of phospholipase A2 activation by phosphatidic acid endogenously formed through phospholipase D action in rat peritoneal mast cell
T. Sato (1993)
Phorbol ester-stimulated exocytosis in lacrimal gland: PKC might not be the sole effector.
D. Zoukhri (1993)
Leukotriene B4 stimulation of an early elevation of phosphatidic acid mass in human neutrophils
J. Tou (2006)
Inhibition by phospholipids, lysophospholipids and gangliosides of melittin-induced phosphorylation in bovine mammary gland.
N. Katoh (1995)
Phosphatidic Acid-mediated Phosphorylation of the NADPH Oxidase Component p47-phox
K. Waite (1997)
Advances in Brief Activation of Phospholipase D Participates in Signal Transduction Pathways Responsive to ' y-Radiation
Matías A. Avila (2007)
Purification and Characterization of a Novel Plasma Membrane Phosphatidate Phosphohydrolase from Rat Liver (*)
D. W. Waggoner (1995)
Depression of cardiac sarcolemmal phospholipase D activity by oxidant-induced thiol modification.
J. Dai (1992)
GTPγS and phorbol ester act synergistically to stimulate both Ca2+‐independent secretion and phospholipase D activity in permeabilized human platelets
J. Coorssen (1993)
Insulin mediators and the mechanism of insulin action.
G. Romero (1993)See more