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Measurement Of Ligand-receptor Interactions.

C. Helm, W. Knoll, J. Israelachvili
Published 1991 · Chemistry, Medicine

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One distinguishing feature of "life" is that the physical forces between biological molecules and membrane surfaces are often highly specific, in contrast to nonspecific interactions such as van der Waals, hydrophobic, and electrostatic (Coulombic) forces. We have used the surface-forces-apparatus technique to study the specific "lock and key" or "ligand-receptor" interaction between two model biomembrane surfaces in aqueous solution. The membranes were lipid bilayers supported on mica surfaces; one carrying streptavidin receptors, the other exposing biotin ligand groups. We found that, although no unusual or specific interaction occurs between two avidin or two biotin surfaces, an avidin and a biotin surface exhibit a very strong, very short-range (less than 1 nm) attraction and that the binding mechanism involves equally specific molecular rearrangements. The results also show that highly specific biological interactions such as are involved in immunological recognition and cell-cell contacts may be studied at the molecular level and in real time by the surface-forces-apparatus technique.
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Influence of glutaraldehyde fixation of cells adherent to solid substrata on their detachment during exposure to shear stress
T. G. Kooten (2008)
Receptor-ligand interactions: Advanced biomedical applications.
I. Gur’yanov (2016)
Bovine Serum Albumin Adhesion Force Measurements Using an Atomic Force Microscopy
C. C. Lai (2006)
Adhesion forces between individual ligand-receptor pairs.
E. Florin (1994)
Multiplexed lipid dip-pen nanolithography on subcellular scales for the templating of functional proteins and cell culture.
S. Sekula (2008)
Phospholipid arrays on porous polymer coatings generated by micro-contact spotting
Sylwia Sekula-Neuner (2017)
Microtribology and Microrheology of Molecularly Thin Liquid Films
A. Berman (2001)
Design fabrication and calibration of MEMS actuators for in-situ materials testing
K. Abbas (2009)
Ligand-receptor interactions
P. Bongrand (1999)
Multi-Step Fibrinogen Binding to the Integrin αIIbβ3 Detected Using Force Spectroscopy
R. Litvinov (2005)
Efficacy Interactions of PEG-DOX-N-acetyl Glucosamine Prodrug Conjugate for Anticancer Therapy.
Smita K. Pawar (2015)
Micromachined force sensors for the study of cell mechanics
S. Yang (2005)
Pattern Formation in Membranes with Quenched Disorder
Sina Sadeghi (2015)
A modular approach for multifunctional polymersomes with controlled adhesive properties.
J. Petit (2018)
Engineering tubulin: microtubule functionalization approaches for nanoscale device applications
Jennelle L. Malcos (2011)
Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion.
A. Chen (2000)
Direct force measurements of specific and nonspecific protein interactions.
D. Leckband (1994)
Quantifying the Limits of Detection of Surface-Enhanced Infrared Spectroscopy with Grating Order-Coupled Nanogap Antennas
Aurelian John-Herpin (2018)
Free energies of protein adsorption onto mineral particles — from the initial encounter to the onset of hysteresis
C. V. Oss (2001)
Carbohydrate-to-carbohydrate interaction in basic cell biology: a brief overview.
S. Hakomori (2004)
Tracking Membrane Protein Association in Model Membranes
M. Reffay (2009)
Principles Involved in Interpreting Single-Molecule Force Measurement of Biomolecules
Sithara Wijeratne (2013)
Specific recognition of macroscopic objects by the cell surface: evidence for a receptor density threshold revealed by micrometric particle binding characteristics.
S. Sarda (2004)
Adhesion of mussel foot protein Mefp-5 to mica: an underwater superglue.
E. Danner (2012)
Nanoscale manipulation and studies of individual biomolecules and DNA-based nanostructures
N. C. Harris (2009)
Specific Recognition at Functionalized Interfaces: Direct Force Measurements of Biomolecular Interactions
D. Leckband (1995)
Interfaces and thin films as seen by bound electromagnetic waves.
W. Knoll (1998)
Immobilization of functionalized lipids in a random poly(methacrylate) copolymer monolayer
K. Lowack (1995)
On the adhesion dynamics of Plasmodium falciparum infected red blood cells
Hodges Emma (2017)
Application of polymer-embedded proteins to fabrication of DNA array.
H. Miyachi (2000)
Translational bioadhesion research: embracing biology without tokenism
J. Waite (2019)
Simulation of cell rolling and adhesion on surfaces in shear flow: general results and analysis of selectin-mediated neutrophil adhesion.
D. Hammer (1992)
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