Online citations, reference lists, and bibliographies.
← Back to Search

Discovery And Analysis Of Inflammatory Disease-related Genes Using CDNA Microarrays.

R. Heller, M. Schena, A. Chai, D. Shalon, T. Bedilion, J. Gilmore, D. Woolley, R. Davis
Published 1997 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
cDNA microarray technology is used to profile complex diseases and discover novel disease-related genes. In inflammatory disease such as rheumatoid arthritis, expression patterns of diverse cell types contribute to the pathology. We have monitored gene expression in this disease state with a microarray of selected human genes of probable significance in inflammation as well as with genes expressed in peripheral human blood cells. Messenger RNA from cultured macrophages, chondrocyte cell lines, primary chondrocytes, and synoviocytes provided expression profiles for the selected cytokines, chemokines, DNA binding proteins, and matrix-degrading metalloproteinases. Comparisons between tissue samples of rheumatoid arthritis and inflammatory bowel disease verified the involvement of many genes and revealed novel participation of the cytokine interleukin 3, chemokine Gro alpha and the metalloproteinase matrix metallo-elastase in both diseases. From the peripheral blood library, tissue inhibitor of metalloproteinase 1, ferritin light chain, and manganese superoxide dismutase genes were identified as expressed differentially in rheumatoid arthritis compared with inflammatory bowel disease. These results successfully demonstrate the use of the cDNA microarray system as a general approach for dissecting human diseases.
This paper references
Chemokine expression in rheumatoid arthritis (RA): evidence of RANTES and macrophage inflammatory protein (MIP)‐1β production by synovial T cells
E. Robinson (1995)
Cytokines in chronic inflammatory arthritis. VI. Analysis of the synovial cells involved in granulocyte-macrophage colony-stimulating factor production and gene expression in rheumatoid arthritis and its regulation by IL-1 and tumor necrosis factor-alpha.
J. Alvaro-Gracia (1991)
Cytokines: coordinators of immune and inflammatory responses.
K. Arai (1990)
Establishment of a human cell line (mono mac 6) with characteristics of mature monocytes
H. W. Löms Ziegler‐Heitbroc (1988)
Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.
M. Schena (1996)
Quantitative analysis of cytokine gene expression in rheumatoid arthritis.
G. Firestein (1990)
A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.
D. Shalon (1996)
The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes.
T. Geiser (1993)
Rheumatoid arthritis. Pathophysiology and implications for therapy.
E. Harris (1990)
Matrix metalloproteinases: a review.
H. Birkedal-Hansen (1993)
Melanoma growth-stimulatory activity/GRO decreases collagen expression by human fibroblasts. Regulation by C-X-C but not C-C cytokines.
E. Unemori (1993)
Molecular cloning of the interleukin-1 beta converting enzyme.
D. Cerretti (1992)
Cytokine Control of Interstitial Collagenase and Collagenase-3 Gene Expression in Human Chondrocytes*
P. Borden (1996)
Role for NF-κB in the Regulation of Ferritin H by Tumor Necrosis Factor-α (*)
E. Kwak (1995)
Processing and secretion of tumor necrosis factor alpha in endotoxin-treated Mono Mac 6 cells are dependent on phorbol myristate acetate.
A. Pradines-Figuères (1992)
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
M. Schena (1995)
Induction of apoptosis in fibroblasts by IL-1β-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3
M. Miura (1993)

This paper is referenced by
DNA array reveals altered gene expression in response to focal cerebral ischemia
Youngdoo Kim (2002)
Proteomics and genomics
W. Rożek (2008)
Profiling of differentially expressed genes in human primary cervical cancer by complementary DNA expression array.
C. Shim (1998)
Verfahren zur erkennung akuter generalisierter entzündlicher zustände (sirs), sepsis, sepsis ähnlichen zuständen und systemischen infektionen
S. Russwurm (2004)
Colorimetric silver detection of DNA microarrays.
I. Alexandre (2001)
Scanning microarrays at multiple intensities enhances discovery of differentially expressed genes
David S. Skibbe (2006)
Probe generation directly from small numbers of cells for DNA microarray studies.
C. Xiang (2003)
Microfluidic Analysis and Parallel Confocal Detection of Single Molecules
M. Goesch (2003)
DNA microarray technology reveals similar gene expression patterns in rats with vitamin a deficiency and chemically induced colitis.
T. Nur (2002)
Systemic macrophage activation in locally-induced experimental arthritis.
J. Simon (2001)
The Application of High Density Microarray for Analysis of Mitogenic Signaling and Cell-Cycle in the Adrenal
C. Wang (2000)
Functional genomics: expression analysis of Escherichia coli growing on minimal and rich media.
H. Tao (1999)
Analysing gene expression data from DNA microarrays to identify candidate genes
T. D. Wu (2001)
DNA hybridization arrays: a powerful technology for nutritional and obesity research.
M. Moreno-Aliaga (2001)
Analysing genetic information with DNA arrays.
S. Case-Green (1998)
Two Candidate Downstream Target Genes for E2A-HLF
H. Kurosawa (1999)
β-Carotene attenuates LPS-induced rat intestinal inflammation via modulating autophagy and regulating the JAK2/STAT3 and JNK/p38 MAPK signaling pathways.
Y. Yang (2020)
Microarray Analysis of Differential Gene Expression Profile in Peripheral Blood Cells of Patients with Human Essential Hypertension
Melvin T. Korkor (2011)
Microarrays: Applications in Environmental Microbiology
J. Zhou (2003)
Oligonucleotide Chips for Expression Analysis: Principles and Practical Procedures
P. Casellas (2001)
Functional genomics in the post-genome era.
R. Woychik (1998)
A systematic molecular genetic approach to study mammalian germline development.
K. Abe (1998)
Detecting early-warning signals for sudden deterioration of complex diseases by dynamical network biomarkers
L. Chen (2012)
DNA Chips Come of Age
G. Ramsay (2003)
Immunotoxicological Study of Depleted Uranium
B. Wan (2004)
Verfahren zur Identifizierung und Diagnose von sowie vohersage des Überlebens bei Lymphomen
L. Staudt (2004)
Identification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model
J. Shou (2006)
Options available — from start to finish — for obtaining expression data by microarray
D. Bowtell (1999)
Cisplatin-digoxigenin mRNA labeling for nonradioactive detection of mRNA hybridized onto nucleic acid cDNA arrays.
T. Hoevel (1999)
Applications of biochip and microarray systems in pharmacogenomics.
K. Jain (2000)
Altered patterns of gene expression distinguishing ascending aortic aneurysms from abdominal aortic aneurysms: complementary DNA expression profiling in the molecular characterization of aortic disease.
T. Absi (2003)
Du génome au protéome: stratégies pour comprendre la complexité de la polyarthrite rhumatoïde
G. Falgarone (2004)
See more
Semantic Scholar Logo Some data provided by SemanticScholar