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Mechanism Of Recruitment Of DnaB Helicase To The Replication Origin Of The Plasmid PSC101.

H. J. Datta, G. S. Khatri, D. Bastia
Published 1999 · Medicine, Biology

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Although many bacterial chromosomes require only one replication initiator protein, e.g., DnaA, most plasmid replicons depend on dual initiators: host-encoded DnaA and plasmid-encoded Rep initiator protein for replication initiation. Using the plasmid pSC101 as a model system, this work investigates the biological rationale for the requirement for dual initiators and shows that the plasmid-encoded RepA specifically interacts with the replicative helicase DnaB. Mutations in DnaB or RepA that disrupt RepA-DnaB interaction cause failure to load DnaB to the plasmid ori in vitro and to replicate the plasmid in vivo. Although, interaction of DnaA with DnaB could not substitute for RepA-DnaB interaction for helicase loading, DnaA along with integration host factor, DnaC, and RepA was essential for helicase loading. Therefore, DnaA is indirectly needed for helicase loading. Instead of a common surface of interaction with initiator proteins, interestingly, DnaB helicase appears to have at least a limited number of nonoverlapping surfaces, each of which interacts specifically with a different initiator protein.
This paper references
10.1016/0092-8674(84)90540-3
Structural and functional analysis of the par region of the pSC101 plasmid
W. T. Tucker (1984)
10.1128/JB.171.4.2056-2065.1989
Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: characterization of pSC101 mutants that replicate in the absence of IHF.
D. Biek (1989)
10.1016/0076-6879(95)62034-6
[32] Radiolabeling of proteins for biochemical studies
Z. Kelman (1995)
10.1016/S0092-8674(00)81039-9
Coupling of a Replicative Polymerase and Helicase: A τ–DnaB Interaction Mediates Rapid Replication Fork Movement
S. Kim (1996)
10.1074/JBC.273.14.8447
Replication Origin of the Broad Host Range Plasmid RK2
K. Doran (1998)
10.1073/PNAS.93.11.5522
The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB.
P. V. Ratnakar (1996)
10.1101/GAD.5.8.1453
Cooperativity at a distance promoted by the combined action of two replication initiator proteins and a DNA bending protein at the replication origin of pSC101.
T. T. Stenzel (1991)
DNA Replication (Freeman, San Francisco)
A. Kornberg (1992)
Host virus interactions in the initiation of bacteriophage lambda DNA replication. Recruitment of Escherichia coli DnaB helicase by lambda P replication protein.
J. Mallory (1990)
10.1073/PNAS.93.23.12902
Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA.
Y. Lu (1996)
10.1093/NAR/11.16.5645
The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.
G. Churchward (1983)
10.1073/PNAS.80.21.6557
Primary structure of the essential replicon of the plasmid pSC101.
C. Vocke (1983)
10.1073/pnas.72.3.921
Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.
S. Wickner (1975)
10.1093/emboj/17.17.5192
Mechanistic studies of initiator–initiator interaction and replication initiation
Y. Lu (1998)
10.1074/jbc.272.32.20173
Role of TrfA and DnaA Proteins in Origin Opening during Initiation of DNA Replication of the Broad Host Range Plasmid RK2*
I. Konieczny (1997)
DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli.
J. Marszalek (1994)
10.1016/0092-8674(87)90547-2
The integration host factor of Escherichia coli binds to bent DNA at the origin of replication of the plasmid pSC101
T. T. Stenzel (1987)
10.1016/S0092-8674(00)80163-4
Replisome Assembly Reveals the Basis for Asymmetric Function in Leading and Lagging Strand Replication
A. Yuzhakov (1996)
Replication of plasmid R6K origin gamma in vitro. Dependence on dual initiator proteins and inhibition by transcription.
T. MacAllister (1991)
10.1128/JB.137.3.1095-1099.1979
Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12.
K. Hasunuma (1979)
10.1128/JB.177.22.6657-6665.1995
Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.
M. Sutton (1995)
The ABC-primosome. A novel priming system employing dnaA, dnaB, dnaC, and primase on a hairpin containing a dnaA box sequence.
H. Masai (1990)
10.1128/JB.169.9.4124-4127.1987
Overproduction of Escherichia coli integration host factor, a protein with nonidentical subunits.
H. Nash (1987)
10.1128/JB.169.10.4703-4709.1987
DnaA protein is required for replication of the minimal replicon of the broad-host-range plasmid RK2 in Escherichia coli.
P. J. Gaylo (1987)
10.1016/0092-8674(83)90183-6
DNA-protein interaction at the origin of DNA replication of the plasmid pSC101
C. Vocke (1983)
Datta et al
S. Kim (1999)
10.1111/j.1365-2958.1991.tb02103.x
The replication of plasmid pSC101
D. Manen (1991)
10.1073/PNAS.70.5.1293
Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants.
S. Cohen (1973)
10.1016/0378-1119(84)90181-1
The replication origin of pSC101: the nucleotide sequence and replication functions of the ori region
Yamaguchi Kazuo (1984)
10.1073/PNAS.89.19.8923
Monomers and dimers of the RepA protein in plasmid pSC101 replication: domains in RepA.
D. Manen (1992)
10.1073/PNAS.82.8.2252
The replication initiator protein of plasmid pSC101 is a transcriptional repressor of its own cistron.
C. Vocke (1985)
10.1128/JB.171.4.2066-2074.1989
Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: mutations in the topA gene allow pSC101 replication in the absence of IHF.
D. Biek (1989)



This paper is referenced by
10.1016/J.MOLCEL.2005.10.037
Oligomeric initiator protein-mediated DNA looping negatively regulates plasmid replication in vitro by preventing origin melting.
S. Zzaman (2005)
10.1073/pnas.0805662105
Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading
M. Rajewska (2008)
10.1099/MIC.0.28294-0
Characterization of the theta-type plasmid pCD3.4 from Carnobacterium divergens, and modulation of its host range by RepA mutation.
M. V. van Belkum (2006)
10.1111/J.1574-6976.2002.TB00620.X
The bacterial replication initiator DnaA. DnaA and oriC, the bacterial mode to initiate DNA replication.
W. Messer (2002)
10.1074/JBC.M400021200
Reconstitution of F Factor DNA Replication in Vitro with Purified Proteins*
S. Zzaman (2004)
10.1371/journal.pgen.1007426
Chromosome 1 licenses chromosome 2 replication in Vibrio cholerae by doubling the crtS gene dosage
R. Ramachandran (2018)
10.1128/AEM.66.9.3856-3867.2000
Characterization of the Minimal Replicon of a Cryptic Deinococcus radiodurans SARK Plasmid and Development of Versatile Escherichia coli-D. radiodurans Shuttle Vectors
R. Meima (2000)
10.1016/J.PLASMID.2004.11.001
Plasmid host-range: restrictions to F replication in Pseudomonas.
Z. Zhong (2005)
10.1073/pnas.1532393100
A multifunctional plasmid-encoded replication initiation protein both recruits and positions an active helicase at the replication origin
Y. Jiang (2003)
10.1016/S0378-1119(99)00434-5
A set of temperature sensitive-replication/-segregation and temperature resistant plasmid vectors with different copy numbers and in an isogenic background (chloramphenicol, kanamycin, lacZ, repA, par, polA).
T. Hashimoto-Gotoh (2000)
supplement: compendium of origins and proteins
C. Weigel (2006)
10.3389/fmolb.2016.00039
Replisome Assembly at Bacterial Chromosomes and Iteron Plasmids
K. Węgrzyn (2016)
10.1016/J.PLASMID.2006.04.002
Interaction of initiator proteins with the origin of replication of an IncL/M plasmid.
Ln Borrell (2006)
10.1074/JBC.M000552200
Interactions of DnaA Proteins from Distantly Related Bacteria with the Replication Origin of the Broad Host Range Plasmid RK2*
R. Caspi (2000)
10.1128/JB.01259-08
An essential DnaB helicase of Bacillus anthracis: identification, characterization, and mechanism of action.
E. Biswas (2009)
10.1046/j.1365-2958.2000.02096.x
The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli
H. Seitz (2000)
10.1128/JB.187.4.1227-1237.2005
Identification and characterization of a novel allele of Escherichia coli dnaB helicase that compromises the stability of plasmid P1.
R. Slavcev (2005)
10.1074/jbc.M100255200
DnaA Box Sequences as the Site for Helicase Delivery during Plasmid RK2 Replication Initiation in Escherichia coli *
M. Pacek (2001)
10.1074/jbc.M109.067348
Replication Initiation at a Distance
Mukesh Saxena (2009)
10.1016/J.PLASMID.2006.05.006
IncP-9 replication initiator protein binds to multiple DNA sequences in oriV and recruits host DnaA protein.
R. Krasowiak (2006)
10.1074/JBC.M010118200
The RepE Initiator Is a Double-stranded and Single-stranded DNA-binding Protein That Forms an Atypical Open Complex at the Onset of Replication of Plasmid pAMβ1 from Gram-positive Bacteria*
E. Le Chatelier (2001)
10.1074/jbc.M310917200
Plasmid P1 RepA Is Homologous to the F Plasmid RepE Class of Initiators*
S. Sharma (2004)
10.1073/pnas.0609046103
Crystal structure of π initiator protein–iteron complex of plasmid R6K: Implications for initiation of plasmid DNA replication
M. Swan (2006)
10.1093/nar/gku453
Sequence-specific interactions of Rep proteins with ssDNA in the AT-rich region of the plasmid replication origin
K. Węgrzyn (2014)
10.1006/JMBI.2001.4741
DnaA boxes in the P1 plasmid origin: the effect of their position on the directionality of replication and plasmid copy number.
K. Park (2001)
10.1128/microbiolspec.PLAS-0026-2014
Iteron Plasmids.
I. Konieczny (2014)
10.1074/JBC.M308516200
Reconstitution of R6K DNA Replication in Vitro Using 22 Purified Proteins*
M. Abhyankar (2003)
10.1128/9781555817732.CH2
Participating Elements in the Replication of Iteron-Containing Plasmids
R. Krüger (2004)
10.1371/journal.pone.0004641
Genome Sequence of the Pathogenic Intestinal Spirochete Brachyspira hyodysenteriae Reveals Adaptations to Its Lifestyle in the Porcine Large Intestine
M. Bellgard (2009)
10.1093/NAR/GKH628
DnaG interacts with a linker region that joins the N- and C-domains of DnaB and induces the formation of 3-fold symmetric rings.
J. Thirlway (2004)
10.1128/JVI.74.9.4057-4063.2000
The Interaction of Bacteriophage P2 B Protein with Escherichia coli DnaB Helicase
R. Odegrip (2000)
10.1006/PLAS.2001.1560
DNA sequence and functional analysis of Lactobacillus delbrueckii subsp. lactis plasmids pN42 and pJBL2.
Aude A Bourniquel (2002)
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