← Back to Search
Transgenic Tobacco Plants With Reduced Capability To Detoxify Reactive Oxygen Intermediates Are Hyperresponsive To Pathogen Infection.
R. Mittler, E. Herr, B. L. Orvar, W. Van Camp, H. Willekens, D. Inzé, B. Ellis
Published 1999 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Reactive oxygen intermediates (ROI) play a critical role in the defense of plants against invading pathogens. Produced during the "oxidative burst," they are thought to activate programmed cell death (PCD) and induce antimicrobial defenses such as pathogenesis-related proteins. It was shown recently that during the interaction of plants with pathogens, the expression of ROI-detoxifying enzymes such as ascorbate peroxidase (APX) and catalase (CAT) is suppressed. It was suggested that this suppression, occurring upon pathogen recognition and coinciding with an enhanced rate of ROI production, plays a key role in elevating cellular ROI levels, thereby potentiating the induction of PCD and other defenses. To examine the relationship between the suppression of antioxidative mechanisms and the induction of PCD and other defenses during pathogen attack, we studied the interaction between transgenic antisense tobacco plants with reduced APX or CAT and a bacterial pathogen that triggers the hypersensitive response. Transgenic plants with reduced capability to detoxify ROI (i.e., antisense APX or CAT) were found to be hyperresponsive to pathogen attack. They activated PCD in response to low amounts of pathogens that did not trigger the activation of PCD in control plants. Our findings support the hypothesis that suppression of ROI-scavenging enzymes during the hypersensitive response plays an important role in enhancing pathogen-induced PCD.
This paper references
Systemic Acquired Resistance.
J. Ryals (1996)
Sacrifice in the face of foes: pathogen-induced programmed cell death in plants.
R. Mittler (1996)
Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants
H. Willekens (1997)
Programmed Cell Death in Plants.
R. Pennell (1997)
Development of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels.
H. Takahashi (1997)
Inhibition of Programmed Cell Death in Tobacco Plants during a Pathogen-Induced Hypersensitive Response at Low Oxygen Pressure.
R. Mittler (1996)
Signal perception and transduction in plant defense responses.
Y. Yang (1997)
Arabidopsis mutants compromised for the control of cellular damage during pathogenesis and aging.
J. Greenberg (1993)
Transgenic tobacco with a reduced catalase activity develops necrotic lesions and induces pathogenesis‐related expression under high light
S. Chamnongpol (1996)
The Hypersensitive Response Reaction in Plants to Pathogens : A Resistance Phenomenon ( Am
R. N. Goodman (1994)
The Metal-Insulator Transition in Correlated Disordered Systems
E. Abrahams (1996)
Salicylate, superoxide synthesis and cell suicide in plant defence
J. Draper (1997)
Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death).
M. Peitsch (1993)
Reactive Oxygen Intermediates Mediate a Systemic Signal Network in the Establishment of Plant Immunity
M. E. Alvarez (1998)
Two Distinct Sources of Elicited Reactive Oxygen Species in Tobacco Epidermal Cells.
A. Allan (1997)
Resistance gene-dependent plant defense responses.
K. Hammond-Kosack (1996)
A Novel Zinc Finger Protein Is Encoded by the Arabidopsis LSD1 Gene and Functions as a Negative Regulator of Plant Cell Death
R. Dietrich (1997)
Reactive oxygen intermediates as mediators of programmed cell death in plants and animals.
T. Jabs (1999)
Post-Transcriptional Suppression of Cytosolic Ascorbate Peroxidase Expression during Pathogen-Induced Programmed Cell Death in Tobacco
R. Mittler (1998)
Programmed cell death: a way of life for plants.
J. Greenberg (1996)
Tobacco class I and II catalases are differentially expressed during elicitor-induced hypersensitive cell death and localized acquired resistance
S. Dorey (1998)
Death Don't Have No Mercy: Cell Death Programs in Plant-Microbe Interactions.
J. Dangl (1996)
Purification and characterization of pea cytosolic ascorbate peroxidase.
R. Mittler (1991)
Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis.
S. Karpiński (1999)
Inhibition of ascorbate peroxidase by salicylic acid and 2,6-dichloroisonicotinic acid, two inducers of plant defense responses.
J. Durner (1995)
The hypersensitive reaction in plants to pathogens: a resistance phenomenon.
R. Goodman (1994)
Incompatible pathogen infection results in enhanced reactive oxygen and cell death responses in transgenic tobacco expressing a hyperactive mutant calmodulin
S. Harding (1998)
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
Alex Levine (1994)
Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid.
Z. Chen (1993)
Defense activation and enhanced pathogen tolerance induced by H2O2 in transgenic tobacco.
S. Chamnongpol (1998)
Initiation of Runaway Cell Death in an Arabidopsis Mutant by Extracellular Superoxide
T. Jabs (1996)
Pathogen-induced programmed cell death in tobacco.
R. Mittler (1997)
Active Oxygen Species in Plant Defense against Pathogens
M. Mehdy (1994)
Hydrogen Peroxide Stimulates Salicylic Acid Biosynthesis in Tobacco
J. León (1995)
Transgenic tobacco plants expressing antisense RNA for cytosolic ascorbate peroxidase show increased susceptibility to ozone injury
B. L. Örvar (1997)
Active oxygen in plant pathogenesis.
C. J. Baker (1995)
This paper is referenced by
Are diverse signalling pathways integrated in the regulation of arabidopsis antioxidant defence gene expression in response to excess excitation energy?
P. Mullineaux (2000)
Hydrogen peroxide induces programmed cell death features in cultured tobacco BY-2 cells, in a dose-dependent manner.
V. Houot (2001)
Oxidative stress, antioxidants and stress tolerance.
R. Mittler (2002)
Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death.
I. Murgia (2004)
Contribution of jasmonic acid to resistance against Phytophthora blight in Capsicum annuum cv. SCM334
Motoko Ueeda (2005)
Enhanced Tolerance to Environmental Stress in Transgenic Plants Expressing the Transcriptional Coactivator Multiprotein Bridging Factor 1c1[w]
N. Suzuki (2005)
Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses.
S. Lee (2007)
Modulation of root antioxidant status to delay cassava post-harvest physiological deterioration
M. Page (2009)
Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent Metschnikowia fructicola.
V. Hershkovitz (2012)
Monodehydroascorbate reductase gene, regulated by the wheat PN-2013 miRNA, contributes to adult wheat plant resistance to stripe rust through ROS metabolism.
H. Feng (2014)
Redox homeostasis via gene families of ascorbate-glutathione pathway
Prachi Pandey (2015)
Defensive strategies of ROS in Programmed Cell Death associated with hypertensive response in plant pathogenesis
Nivedita Dey (2020)
Correction: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
Youxiong Que (2015)
Compensation for lack of a cytosolic ascorbate peroxidase in an Arabidopsis mutant by activation of multiple antioxidative systems
N. Asai (2004)
The elicitor-induced oxidative processes in leaves of Solanum species with differential polygenic resistance to Phytophthora infestans.
L. Polkowska-Kowalczyk (2004)
Efeito do estresse pelo frio sobre células embriogênicas de Araucaria angustifolia em cultura: disfunção mitocondrial e estresse oxidativo
Caroline Valente (2012)
Investigation of the Arabidopsis nonhost resistance mechanism against the soybean pathogen, Phytophthora sojae
Rishi Sumit (2013)
Effects of ZnCl2 on ROS generation, plasma membrane properties, and changes in protein expression in grapevine root explants
Vladimír Řepka (2016)
TaTypA, a Ribosome-Binding GTPase Protein, Positively Regulates Wheat Resistance to the Stripe Rust Fungus
Peng Liu (2016)
Disease Resistance Mechanisms in Plants
Ethan J. Andersen (2018)
BIOCHEMICAL, PHYSIOLOGICAL, AND ANATOMICAL INSIGHTS INTO APHID-BIOENERGY SWITCHGRASS INTERACTIONS
T. Prochaska (2015)
Role of Nitric oxide in regulation of H 2 O 2 mediating tolerance of plants to abiotic stress : A synergistic signalling approach
M. Mazid (2011)
Scopoletin expression in elicitor-treated and tobacco mosaic virus-infected tobacco plants.
L. Costet (2002)
The role of peroxisomes in the integration of metabolism and evolutionary diversity of photosynthetic organisms.
A. Igamberdiev (2002)
REACTIVE OXYGEN SPECIES: Metabolism,
K. Apel (2004)
Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances Paraquat-induced photooxidative stress and Nitric Oxide-induced cell death
D. Tarantino (2005)
Determining the storage potential of iceberg lettuce with chlorophyll fluorescence
R. Schofield (2005)
TransientSuppressionofaCatalaseand an AlternativeOxidaseGeneDuringVirus-induced LocalLesionFormation(HypersensitiveResponse) isIndependentoftheExtentofLeafNecrotization
A. Künstler (2007)
Plum pox virus (PPV) infection produces an imbalance on the antioxidative systems in Prunus species
J. A. Hernández (2007)
Reactive oxygen intermediates and oxalic acid in the pathogenesis of the necrotrophic fungus Sclerotinia sclerotiorum
A. Walz (2007)
RESEARCH ARTICLE CHANGES IN PEROXIDASE ACTIVITY DURING NATURAL AND OXIDATIVE STRESS-INDUCED SENESCENCE OF Azadirachta indica A. juss DETACHED LEAVES
Prashanth B. Goud (2013)
Catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis
Orsolya Viczián (2014)See more