Online citations, reference lists, and bibliographies.
← Back to Search

Monolayers Of Derivatized Poly(L-lysine)-grafted Poly(ethylene Glycol) On Metal Oxides As A Class Of Biomolecular Interfaces.

L. Ruiz-Taylor, T. L. Martin, F. Zaugg, K. Witte, P. Indermuhle, S. Nock, P. Wagner
Published 2001 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly(l-lysine)-grafted poly(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-derivatized poly(l-lysine)-grafted poly(ethylene glycol) copolymers, PLL-g-[(PEGm)((1-x)) (PEG-biotin)(x)], where x varies from 0 to 1. Monolayers were produced on titanium dioxide substrates and characterized by x-ray photoelectron spectroscopy. The specific biorecognition properties of these biotinylated surfaces were investigated with the use of radiolabeled streptavidin alone and within complex protein mixtures. The PLL-g-PEG-biotin monolayers specifically capture streptavidin, even from a complex protein mixture, while still preventing nonspecific adsorption of other proteins. This streptavidin layer can subsequently capture biotinylated proteins. Finally, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this class of biomolecular interfaces for applications based on protein patterning.
This paper references
10.1021/J100082A031
An Intrinsic Relationship between Molecular Structure in Self-Assembled n-Alkylsiloxane Monolayers and Deposition Temperature
A. Parikh (1994)
10.1021/JA973071F
Microfluidic Networks for Chemical Patterning of Substrates: Design and Application to Bioassays
E. Delamarche (1998)
10.1016/S0006-3495(96)79810-7
Covalent immobilization of native biomolecules onto Au(111) via N-hydroxysuccinimide ester functionalized self-assembled monolayers for scanning probe microscopy.
P. Wagner (1996)
10.1016/0076-6879(90)84259-J
Avidin and streptavidin.
Green Nm (1990)
10.1021/LA981758N
Highly Oriented, Self-Assembled Alkanephosphate Monolayers on Tantalum(V) Oxide Surfaces
D. Brovelli (1999)
10.1016/0076-6879(71)18342-5
[74] Spectrophotometric determination of avidin and biotin
N. Green (1970)
An Introduction to Organic Thin Films: From LangmuirBlodgett to Self-Assembly (Academic, San Diego)
A. Ulman (1991)
10.1007/BF02916421
Preparation of immobilized proteins covalently coupled through silane coupling agents to inorganic supports
H. Weetall (1993)
10.1021/JA00076A032
Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide): a model system using self-assembled monolayers
K. Prime (1993)
10.1016/S1074-5521(98)90062-X
Self-assembly and steric stabilization at heterogeneous, biological surfaces using adsorbing block copolymers.
D. Elbert (1998)
Immobilized Affinity Ligand Techniques
G. T. Hermanson (1992)
10.1021/LA990495+
Chemical Modification of Metal Oxide Surfaces in Supercritical CO2: In Situ Infrared Studies of the Adsorption and Reaction of Organosilanes on Silica
J. Combes (1999)
Material properties of absorbable self-reinforced fibrillated poly-96L/4 D-lactide (SR-PLA96) rods; a study in vitro and in vivo.
A. Saikku-Bäckström (1999)
10.1021/JA991049B
Probing resistance to protein adsorption of oligo(ethylene glycol)-terminated self-assembled monolayers by scanning force microscopy
K. Feldman (1999)
10.1021/LA980257Z
Covalent Attachment of Cell-Adhesive, (Arg-Gly-Asp)-Containing Peptides to Titanium Surfaces
S. Xiao (1998)
10.1021/LA990941T
Structural Chemistry of Self-Assembled Monolayers of Octadecylphosphoric Acid on Tantalum Oxide Surfaces
M. Textor (2000)
10.1021/JA00001A002
Formation of self-assembled monolayers by chemisorption of derivatives of oligo(ethylene glycol) of structure HS(CH2)11(OCH2CH2)mOH on gold
C. Pale-Grosdemange (1991)
10.1016/0368-2048(76)80025-4
Relative intensities in photoelectron spectroscopy of atoms and molecules
R. F. Reilman (1976)
Poly(Ethylene Glycol) Chemistry Biotechnical and Biomedical Applications
J. M. Harris (1992)
10.1021/AC9504023
A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance.
G. Sigal (1996)
10.1021/BK-1997-0680
Poly(ethylene glycol): Chemistry and Biological Applications
J. M. Harris (1997)
Affinity biosensors : techniques and protocols
K. Rogers (1998)
10.1021/JP993359M
Poly(l-lysine)-g-Poly(ethylene glycol) Layers on Metal Oxide Surfaces: Attachment Mechanism and Effects of Polymer Architecture on Resistance to Protein Adsorption†
G. Kenausis (2000)
10.1116/1.581507
Surface charge neutralization of insulating samples in x-ray photoemission spectroscopy
P. E. Larson (1998)
10.1021/JP9716952
MOLECULAR CONFORMATION AND SOLVATION OF OLIGO(ETHYLENE GLYCOL)-TERMINATED SELF-ASSEMBLED MONOLAYERS AND THEIR RESISTANCE TO PROTEIN ADSORPTION
R. Wang (1997)
Practical surface analysis: By auger and x-ray photoelectron spectroscopy
David Briggs (1983)
10.1016/S0956-5663(98)00116-X
Immobilization of histidine-tagged proteins on gold surfaces using chelator thioalkanes.
D. Kröger (1999)
10.1126/science.270.5235.467
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
M. Schena (1995)



This paper is referenced by
Fluorescence-based ligand assays for protein detection using affibody affinity proteins
Björn Renberg (2006)
10.1002/9783527610754.FA04
Protein Microarrays: Surface Chemistry and Coupling Schemes
M. Schaeferling (2008)
10.15446/DYNA.V84N202.63963
Immobilization of recognition elements on a self-assembled monolayers bio-platform
Julia Constanza Reyes-Cuellar (2017)
10.1083/jcb.201807119
BAR scaffolds drive membrane fission by crowding disordered domains
W. T. Snead (2019)
10.1016/S0003-2670(03)00632-9
Coupled enzyme reaction microarrays based on pin-printing of sol–gel derived biomaterials
N. Rupcich (2003)
10.1039/B715355B
Chemical and biological functionalization of titanium for dental implants
H. Schliephake (2008)
Measuring binding kinetics of ligands with tethered receptors by fluorescence polarization complemented with total internal reflection fluorescence microscopy
K. C. Kwok (2010)
10.1016/j.aca.2017.01.051
Biofunctional polyelectrolytes assembling on biosensors - A versatile surface coating method for protein detections.
Ziyu Han (2017)
Développement d' outils innovants pour le diagnostic et la découverte de cibles dans le cancer du sein
Klervi Even (2012)
10.1016/S0006-3495(01)75809-2
Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks.
Y. Hiratsuka (2001)
10.1007/978-3-319-14711-6_13
Wireless CMOS Bio-medical SoCs for DNA/Protein/Glucose Sensing
S. Lu (2015)
10.1093/NAR/GNG119
DNA display for in vitro selection of diverse peptide libraries.
M. Yonezawa (2003)
10.1016/j.biomaterials.2009.01.051
The influence of poly(ethylene oxide) grafting via siloxane tethers on protein adsorption.
R. Murthy (2009)
10.1039/c6cs00494f
Nanoplasmonic sensors for biointerfacial science.
Joshua A. Jackman (2017)
10.1002/cmmi.403
Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection.
Fenghua Xu (2011)
10.1002/QSAR.200640083
Small Molecule Microarrays: Applications Using Specially Tagged Chemical Libraries
J. Wang (2006)
10.1063/1.3152446
Adsorption and pinning of multiblock copolymers on chemically heterogeneous patterned surfaces.
K. Sumithra (2009)
10.1089/OMI.2006.10.411
Antibody microarrays: current status and key technological advances.
C. Wingren (2006)
10.1002/anie.200801711
Chemical strategies for generating protein biochips.
P. Jonkheijm (2008)
10.1002/jbm.a.31999
Anticoagulant surface modification of titanium via layer-by-layer assembly of collagen and sulfated chitosan multilayers.
Quanli Li (2009)
10.1002/POLB.20238
Adhesive bonding of glassy polymer surfaces by an ultrathin layer of a semicrystalline polymer
Rachel L. McSwain (2004)
10.1007/s10404-009-0443-4
Surface molecular property modifications for poly(dimethylsiloxane) (PDMS) based microfluidic devices
I. Wong (2009)
10.1007/978-0-387-25843-0_8
From One-Bead One-Compound Combinatorial Libraries to Chemical Microarrays
K. Lam (2006)
Bionalytische erkennungsoberfläche mit optimierter dichte der erkennungselemente
M. Pawlak (2002)
10.1021/acs.analchem.6b02230
Ion-Step Method for Surface Potential Sensing of Silicon Nanowires.
Songyue Chen (2016)
10.1201/9780203911129
Protein Arrays, Biochips, and Proteomics: The Next Phase of Genomic Discovery
J. Albala (2007)
10.1038/nature05741
Weighing of biomolecules, single cells and single nanoparticles in fluid
T. Burg (2007)
10.1126/sciadv.1600881
Kinetic control of the coverage of oil droplets by DNA-functionalized colloids
D. Joshi (2016)
10.1016/J.APSUSC.2009.03.011
Improving blood-compatibility of titanium by coating collagen-heparin multilayers
J. Chen (2009)
10.1016/B978-012507060-7/50026-X
Current Issues and Advances in Dissociated Cell Culturing on Nano-and Microfabricated Substrates
H. Craighead (2003)
10.1016/J.BPC.2004.12.027
Stiff polymer adsorption. Onset to pattern recognition.
J. J. Cerdà (2005)
10.1063/1.2360267
Adsorption of diblock copolymers on stripe-patterned surfaces.
K. Sumithra (2006)
See more
Semantic Scholar Logo Some data provided by SemanticScholar