Nanoparticle Size And Surface Properties Determine The Protein Corona With Possible Implications For Biological Impacts
Martin Lundqvist, Johannes Stigler, Giuliano Elia, Iseult Lynch, Tommy Cedervall, Kenneth A. Dawson
Published 2008 · Materials Science, Medicine
Download PDFAnalyze on Scholarcy
Nanoparticles in a biological fluid (plasma, or otherwise) associate with a range of biopolymers, especially proteins, organized into the “protein corona” that is associated with the nanoparticle and continuously exchanging with the proteins in the environment. Methodologies to determine the corona and to understand its dependence on nanomaterial properties are likely to become important in bionanoscience. Here, we study the long-lived (“hard”) protein corona formed from human plasma for a range of nanoparticles that differ in surface properties and size. Six different polystyrene nanoparticles were studied: three different surface chemistries (plain PS, carboxyl-modified, and amine-modified) and two sizes of each (50 and 100 nm), enabling us to perform systematic studies of the effect of surface properties and size on the detailed protein coronas. Proteins in the corona that are conserved and unique across the nanoparticle types were identified and classified according to the protein functional properties. Remarkably, both size and surface properties were found to play a very significant role in determining the nanoparticle coronas on the different particles of identical materials. We comment on the future need for scientific understanding, characterization, and possibly some additional emphasis on standards for the surfaces of nanoparticles.
This paper references
Proteomic analysis of high-density lipoprotein.
Farhad Rezaee (2006)
Mass spectrometry-based analytical tools for the molecular protein characterization of human plasma lipoproteins.
Manfred Heller (2005)
Analysis of plasma protein adsorption on polymeric nanoparticles with different surface characteristics.
Martin Lück (1998)
Influence of surface charge density on protein adsorption on polymeric nanoparticles: analysis by two-dimensional electrophoresis.
Andrea Gessner (2002)
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
A. Yu. Shevchenko (1996)
Functional groups on polystyrene model nanoparticles: influence on protein adsorption.
Andrea Gessner (2003)
HDL proteomics: pot of gold or Pandora's box?
Muredach P. Reilly (2007)
Proteomic analysis of human very low-density lipoprotein by two-dimensional gel electrophoresis and MALDI-TOF/TOF.
Carmine Mancone (2007)
Influence of Fluorescent Labelling of Polystyrene Particles on Phagocytic Uptake, Surface Hydrophobicity, and Plasma Protein Adsorption
Rainer Helmut Mueller (2004)
Selective IgG subclass deficiency: quantification and clinical relevance.
Royston Jefferis (1990)
Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database.
John R. Yates (1995)
Nanoparticles with decreasing surface hydrophobicities: influence on plasma protein adsorption.
Andrea Gessner (2000)
The Human Plasma Proteome
Nicholas L Anderson (2002)
Lipoproteomics I: mapping of proteins in low-density lipoprotein using two-dimensional gel electrophoresis and mass spectrometry.
Helen Karlsson (2005)
Lipoproteomics II: mapping of proteins in high-density lipoprotein using two-dimensional gel electrophoresis and mass spectrometry.
Helen Karlsson (2005)
Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL.
Tomáš Vaisar (2007)
Detailed identification of plasma proteins adsorbed on copolymer nanoparticles.
Tommy Cedervall (2007)
Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
Tommy Cedervall (2007)
Diverse range of small peptides associated with high-density lipoprotein.
Glen L. Hortin (2006)
This paper is referenced by
Imitation of nature: Bionic design in the study of particle adjuvants.
Jie Wu (2019)
Formation of lipid and polymer based gold nanohybrids using a nanoreactor approach
Dominik Witzigmann (2015)
Probing stem cell behavior using nanoparticle-based approaches.
Sahishnu Patel (2015)
Interfacing proteins with graphitic nanomaterials: from spontaneous attraction to tailored assemblies.
Federica De Leo (2015)
The engineered nanoparticles in food chain: potential toxicity and effects
A. M. Maharramov (2019)
Molecularly Imprinted Nanogels Acquire Stealth In Situ by Cloaking Themselves with Native Dysopsonic Proteins.
Toshifumi Takeuchi (2017)
The potential of protein-nanomaterial interaction for advanced drug delivery.
Qiang Peng (2016)
Anisotropic surface functionalization of Au nanorods driven by molecular architecture and curvature effects.
Estefanía González Solveyra (2016)
Metal Nanomaterial Toxicity Variations Within the Vascular System
Alaeddin Bashir Abukabda (2016)
Cobalt iron oxide nanoparticles induce cytotoxicity and regulate the apoptotic genes through ROS in human liver cells (HepG2).
Maqusood Ahamed (2016)
Engineering the Protein Corona of a Synthetic Polymer Nanoparticle for Broad-Spectrum Sequestration and Neutralization of Venomous Biomacromolecules.
Jeffrey C. O'Brien (2016)
Aggregation morphology is a key factor determining protein adsorption on graphene oxide and reduced graphene oxide nanomaterials
Yu Qi (2019)
What is the impact of surface modifications and particle size on commercial titanium dioxide particle samples? - A review of in vivo pulmonary and oral toxicity studies - Revised 11-6-2018.
David B Warheit (2019)
Imaging Techniques for Probing Nanoparticles in Cells and Skin
Christina Graf (2019)
Metal nanomaterials: Immune effects and implications of physicochemical properties on sensitization, elicitation, and exacerbation of allergic disease
Katherine A Roach (2019)
Influence of protein corona on the transport of molecules into cells by mesoporous silica nanoparticles.
Amauri J. Paula (2013)
Effect of charge regulation and ion-dipole interactions on the selectivity of protein-nanoparticle binding.
Fernando Luís Barroso da Silva (2014)
Statistical methods in single particle fluorescence microscopy
Magnus Röding (2013)
Cytotoxic effects in 3T3-L1 mouse and WI-38 human fibroblasts following 72 hour and 7 day exposures to commercial silica nanoparticles.
Maciej Stępnik (2012)
Interaction of gold nanoparticles with common human blood proteins.
S. De Paoli Lacerda (2010)
Characterizing Topology of Surface Ligands on Liposomes
Si Beum Lee (2018)
David W. Hobson (2016)
Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells.
Sriram Vaidyanathan (2016)
Trapping/Pinning of colloidal microspheres over glass substrate using surface features
Praneet Prakash (2017)
Nanobiomedicine ZnO Nanoparticles : A Promising Anticancer Agent Review
Gunjan Bisht (2016)
Understanding the surface chemistry of ceria nanoparticles using a multi-method approach
Inder Preet Kaur (2015)
3-Helix Micelles as Nanocarriers – Understanding Hierarchical Structures, Kinetic Pathway, and Controlling Multivalency
JooChuan Ang (2016)
Functional Bio-Nano Hybrids Through a Precise Control of Interfacial Interactions at the Nanoscale
Sirimuvva Tadepalli (2017)
Nanoengineered Functional Structures for Photonic and Microfluidic Applications
Long Chen (2017)
Development of reflectance imaging methodologies to investigate super-paramagnetic iron oxide nanoparticles
Emily J Guggenheim (2017)
Aus dem Institut für Transfusionsmedizin der Medizinischen Fakultät Charité – Universitätsmedizin Berlin DISSERTATION One-pot Formulation of Protein Submicron Particles and Their Haemocompatibility zur Erlangung des akademischen Grades Doctor rerum medicinalium (Dr. rer. medic.) vorgelegt der Me
Complexes of Keggin POMs, [PM12O40]3−, M = Mo, W, with GlyGly peptide and Arginine – crystal structures and solution reactivity
Kayly M Rominger (2019)See more