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Nanoparticle Size And Surface Properties Determine The Protein Corona With Possible Implications For Biological Impacts

Martin Lundqvist, Johannes Stigler, Giuliano Elia, Iseult Lynch, Tommy Cedervall, Kenneth A. Dawson
Published 2008 · Materials Science, Medicine
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Nanoparticles in a biological fluid (plasma, or otherwise) associate with a range of biopolymers, especially proteins, organized into the “protein corona” that is associated with the nanoparticle and continuously exchanging with the proteins in the environment. Methodologies to determine the corona and to understand its dependence on nanomaterial properties are likely to become important in bionanoscience. Here, we study the long-lived (“hard”) protein corona formed from human plasma for a range of nanoparticles that differ in surface properties and size. Six different polystyrene nanoparticles were studied: three different surface chemistries (plain PS, carboxyl-modified, and amine-modified) and two sizes of each (50 and 100 nm), enabling us to perform systematic studies of the effect of surface properties and size on the detailed protein coronas. Proteins in the corona that are conserved and unique across the nanoparticle types were identified and classified according to the protein functional properties. Remarkably, both size and surface properties were found to play a very significant role in determining the nanoparticle coronas on the different particles of identical materials. We comment on the future need for scientific understanding, characterization, and possibly some additional emphasis on standards for the surfaces of nanoparticles.
This paper references
Proteomic analysis of high-density lipoprotein.
Farhad Rezaee (2006)
Mass spectrometry-based analytical tools for the molecular protein characterization of human plasma lipoproteins.
Manfred Heller (2005)
Analysis of plasma protein adsorption on polymeric nanoparticles with different surface characteristics.
Martin Lück (1998)
Influence of surface charge density on protein adsorption on polymeric nanoparticles: analysis by two-dimensional electrophoresis.
Andrea Gessner (2002)
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
A. Yu. Shevchenko (1996)
Functional groups on polystyrene model nanoparticles: influence on protein adsorption.
Andrea Gessner (2003)
HDL proteomics: pot of gold or Pandora's box?
Muredach P. Reilly (2007)
Proteomic analysis of human very low-density lipoprotein by two-dimensional gel electrophoresis and MALDI-TOF/TOF.
Carmine Mancone (2007)
Influence of Fluorescent Labelling of Polystyrene Particles on Phagocytic Uptake, Surface Hydrophobicity, and Plasma Protein Adsorption
Rainer Helmut Mueller (2004)
Selective IgG subclass deficiency: quantification and clinical relevance.
Royston Jefferis (1990)
Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database.
John R. Yates (1995)
Nanoparticles with decreasing surface hydrophobicities: influence on plasma protein adsorption.
Andrea Gessner (2000)
The Human Plasma Proteome
Nicholas L Anderson (2002)
Lipoproteomics I: mapping of proteins in low-density lipoprotein using two-dimensional gel electrophoresis and mass spectrometry.
Helen Karlsson (2005)
Lipoproteomics II: mapping of proteins in high-density lipoprotein using two-dimensional gel electrophoresis and mass spectrometry.
Helen Karlsson (2005)
Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL.
Tomáš Vaisar (2007)
Detailed identification of plasma proteins adsorbed on copolymer nanoparticles.
Tommy Cedervall (2007)
Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
Tommy Cedervall (2007)
Diverse range of small peptides associated with high-density lipoprotein.
Glen L. Hortin (2006)

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Qiang Peng (2016)
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S. De Paoli Lacerda (2010)
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Si Beum Lee (2018)
Applied Nanotoxicology.
David W. Hobson (2016)
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Sriram Vaidyanathan (2016)
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