Online citations, reference lists, and bibliographies.
← Back to Search

Replication Of Mitochondrial DNA In Mouse L Cells And Their Thymidine Kinase - Derivatives: Displacement Replication On A Covalently-closed Circular Template.

D. Robberson, D. Clayton
Published 1972 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Thymidine kinase(-) lines of mouse L cells incorporate thymidine exclusively into mitochondrial DNA. This fact permits assessment of labeled mitochondrial DNA components in ethidium bromide-cesium chloride gradients. Contaminating nuclear DNA is unlabeled and need not be removed. Elimination of the DNase treatment of purified mitochondria reveals that the replicative forms that exhibit displacement replication up to full genome size are covalently-closed circular molecules. Denaturation followed by brief renaturation of these larger replicating molecules produces closed-circular DNA with a deficiency of Watson-Crick turns, appearing as single-strand loops. This result suggests that displacement replication proceeds with nicking and rapid closure of the covalently-closed circular template.



This paper is referenced by
10.1007/978-3-642-65578-4
Progress in Molecular and Subcellular Biology 3
A. Braverman (1973)
10.1007/978-1-59745-521-3_12
Measuring mtDNA damage using a supercoiling-sensitive qPCR approach.
Sam W. Chan (2009)
10.1074/jbc.M806174200
Native R-loops Persist throughout the Mouse Mitochondrial DNA Genome*S⃞
T. Brown (2008)
10.1007/978-3-642-65578-4_6
[Mitochondrial DNA of malignant cells].
C. Paoletti (1970)
10.1021/BI00609A014
Biochemical and electron microscopic characterization of DNA-RNA complexes from HeLa cell mitochondria.
D. Carré (1978)
10.1016/B978-0-12-461205-1.50043-6
Mitochondrial DNA Synthesis
M. Guéride (1993)
10.1007/BF00230152
The metabolism of deoxyguanosine in mitochondria. Characterization of the uptake process
L. Watkins (2004)
10.1007/978-3-030-41283-8_6
DNA Repair and Mutagenesis in Vertebrate Mitochondria: Evidence for Asymmetric DNA Strand Inheritance.
B. Matkarimov (2020)
10.1038/277192A0
Nucleotide sequence of a region of human mitochondrial DNA containing the precisely identified origin of replication
S. Crews (1979)
10.1007/978-1-4613-3888-8_7
Replication of Drosophila Mitochondrial DNA
D. Wolstenholme (1983)
10.1021/CR040463D
DNA polymerase gamma in mitochondrial DNA replication and repair.
M. A. Graziewicz (2006)
10.1099/0022-1317-25-3-457
Herpes simplex virus-induced enhancement of mitochondrial DNA synthesis in the absence of virus replication.
K. Radsak (1974)
10.1134/S0006297915110036
Mitochondrial matrix processes
I. Mazunin (2015)
10.1016/B978-0-12-048850-6.50017-X
THE UNIQUE MECHANISM OF ANIMAL MITOCHONDRIAL DNA REPLICATION
D. Clayton (1980)
10.1007/BF00391825
Complex forms of mitochondrial DNA in human B cells transformed by Epstein-Barr virus (EBV)
G. Christiansen (2004)
10.1016/0012-1606(74)90282-6
Replication of mitochondrial DNA in the early development of Misgurnus fossilis.
V. Mikhailov (1974)
10.1016/S0022-2836(76)80036-8
Mechanism of mitochondrial DNA replication in mouse L-cells: topology of circular daughter molecules and dynamics of catenated oligomer formation.
A. Berk (1976)
10.1016/0092-8674(78)90309-4
The transcription map of mouse mitochondrial DNA
J. Battey (1978)
10.1016/0092-8674(82)90049-6
Replication of animal mitochondrial DNA
D. Clayton (1982)
10.1006/EXCR.1999.4763
Vertebrate mitochondrial DNA-a circle of surprises.
D. Clayton (2000)
10.1038/srep13571
Computational DNA hole spectroscopy: A new tool to predict mutation hotspots, critical base pairs, and disease ‘driver’ mutations
Martha Y. Suárez (2015)
10.1007/978-1-4613-3888-8_8
Replication of Mammalian Mitochondrial DNA
D. Clayton (1983)
10.1039/c1lc20317g
A microfluidic system for fast detection of mitochondrial DNA deletion.
C H Chang (2011)
10.1093/nar/gkx395
DNA synthesis determines the binding mode of the human mitochondrial single-stranded DNA-binding protein
J. A. Morin (2017)
10.4049/jimmunol.1300774
The Human Immune System Recognizes Neopeptides Derived from Mitochondrial DNA Deletions
Bhargavi Duvvuri (2014)
Mitochondrial dysfunction and oxidative stress in osteoarthritis
Christos Gavriilidis (2012)
Identifying and defining clinical phenotypes in mitochondrial disease
Y. Ng (2017)
10.1007/BFB0030491
DNA replication in eukaryotes.
Erhard Winersberger (1978)
10.1083/JCB.100.1.251
The rat liver mitochondrial DNA-protein complex: displaced single strands of replicative intermediates are protein coated
G. V. Van Tuyle (1985)
10.1007/978-3-540-69414-4_5
Mitochondriale DNA des Menschen
B. Wissinger (2008)
10.1007/s00018-010-0530-4
Mitochondrial DNA replication and disease: insights from DNA polymerase γ mutations
J. Stumpf (2010)
10.1083/JCB.61.1.14
EVIDENCE FOR DISCONTINUOUS REPLICATION OF CIRCULAR MITOCHONDRIAL DNA MOLECULES FROM NOVIKOFF RAT ASCITES HEPATOMA CELLS
K. Koike (1974)
See more
Semantic Scholar Logo Some data provided by SemanticScholar