Online citations, reference lists, and bibliographies.
← Back to Search

OMTK1, A Novel MAPKKK, Channels Oxidative Stress Signaling Through Direct MAPK Interaction*

H. Nakagami, S. Kiegerl, H. Hirt
Published 2004 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
In common with other eukaryotes, plants utilize mitogen-activated protein kinase (MAPK) cascades to mediate responses to a wide variety of stimuli. In contrast to other eukaryotes, plants have an unusually large number of MAPK components, such as more than 20 MAPKs, 10 MAPK kinases (MAPKKs), and 60 MAPKK kinases (MAPKKKs) in Arabidopsis (MAPK Group (2002) Trends Plant Sci. 7, 301–308). Presently it is mostly unknown how MAPK signaling specificity is generated in plants. Here we have isolated OMTK1 (oxidative stress-activated MAP triple-kinase 1), a novel MAPKKK from alfalfa (Medicago sativa). In plant protoplasts, OMTK1 showed basal kinase activity and was found to induce cell death. Among a panel of hormones and stresses tested, only H2O2 was found to activate OMTK1. Out of four MAPKs, OMTK1 specifically activated MMK3 resulting in an increased cell death rate. Pull-down analysis between recombinant proteins indicated that OMTK1 directly interacts with MMK3 and that OMTK1 and MMK3 are part of a protein complex in vivo. These results indicate that OMTK1 plays a MAPK scaffolding role and functions in activation of H2O2 -induced cell death in plants.
This paper references
Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade.
J. A. Printen (1994)
J. Biol. Chem
Y Zhang (1999)
Mol. Cell. Biol
M Ito (1999)
10.1016/S0168-9525(02)02723-3
Yeast go the whole HOG for the hyperosmotic response.
S. O'Rourke (2002)
10.1093/emboj/cdg131
A MAPK pathway mediates ethylene signaling in plants
F. Ouaked (2003)
10.1101/GAD.8.3.313
The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.
J. E. Kranz (1994)
10.1038/266092b0
… genetics …
B. Bainbridge (1977)
Mol. Cell. Biol
J Cheng (2000)
10.1073/PNAS.97.6.2940
Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants.
Y. Kovtun (2000)
EMBO J
F Ouaked (2003)
10.1105/tpc.12.11.2247
SIMKK, a Mitogen-Activated Protein Kinase (MAPK) Kinase, Is a Specific Activator of the Salt Stress–Induced MAPK, SIMK
S. Kiegerl (2000)
10.1016/S1360-1385(02)02302-6
Mitogen-activated protein kinase cascades in plants: a new nomenclature.
Kazuya Ichimura (2002)
10.1016/S1369-5266(00)00191-6
Plant mitogen-activated protein kinase signaling cascades.
G. Tena (2001)
J. Cell Sci
E A Elion (2001)
Trends Biochem. Sci
A J Whitmarsh (1998)
Biochem. Biophys. Res. Commun
K Ichimura (1998)
10.1126/SCIENCE.281.5383.1668
MP1: a MEK binding partner that enhances enzymatic activation of the MAP kinase cascade.
H. Schaeffer (1998)
10.1128/MCB.20.7.2334-2342.2000
Synergistic Interaction of MEK Kinase 2, c-Jun N-Terminal Kinase (JNK) Kinase 2, and JNK1 Results in Efficient and Specific JNK1 Activation
J. Cheng (2000)
10.1016/S1369-5266(02)00285-6
Complexity, cross talk and integration of plant MAP kinase signalling.
C. Jonak (2002)
10.1105/TPC.010256
Convergence and divergence of stress-induced mitogen-activated protein kinase signaling pathways at the level of two distinct mitogen-activated protein kinase kinases.
F. Cardinale (2002)
10.1074/JBC.274.49.35247
Axin Forms a Complex with MEKK1 and Activates c-Jun NH2-terminal Kinase/Stress-activated Protein Kinase through Domains Distinct from Wnt Signaling*
Y. Zhang (1999)
J. Biol. Chem
F Cardinale (2000)
The Ste5p scaffold.
E. Elion (2001)
10.1105/tpc.11.1.101
A MAP Kinase Is Activated Late in Plant Mitosis and Becomes Localized to the Plane of Cell Division
L. Bögre (1999)
疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
宁北芳 (2005)
10.1126/SCIENCE.281.5383.1671
A mammalian scaffold complex that selectively mediates MAP kinase activation.
A. Whitmarsh (1998)
Curr. Opin. Plant Biol
C Jonak (2002)
Trends Plant Sci
Mapk Group (2002)
10.1016/S0092-8674(00)00116-1
Signal Transduction by the JNK Group of MAP Kinases
R. Davis (2000)
10.1016/0092-8674(94)90427-8
Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae
Kang-Yell Chol (1994)
Science ’ s STKE http : / / stke
M. Ito (2002)
Curr. Opin. Plant Biol
G Tena (2001)
Trends Genet
S M O 'rourke (2002)
Science’s STKE http://stke.sciencemag
T. Raabe (2002)
10.1038/415977a
MAP kinase signalling cascade in Arabidopsis innate immunity
T. Asai (2002)
10.1038/27240
Suppression of auxin signal transduction by a MAPK cascade in higher plants
Y. Kovtun (1998)
10.1016/0092-8674(93)90586-F
Cell
AC Tose (1993)
10.1074/jbc.M007418200
Differential Activation of Four Specific MAPK Pathways by Distinct Elicitors*
F. Cardinale (2000)
Genes Dev
J E Kranz (1994)
Curr. Opin. Plant Biol
S Neill (2002)
10.1074/JBC.M005926200
MEKK1 Binds Raf-1 and the ERK2 Cascade Components*
M. Karandikar (2000)
10.1016/S0968-0004(98)01309-7
Structural organization of MAP-kinase signaling modules by scaffold proteins in yeast and mammals.
A. Whitmarsh (1998)
10.1016/S1369-5266(02)00282-0
Hydrogen peroxide signalling.
S. Neill (2002)
10.1006/BBRC.1998.9796
Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in Arabidopsis.
K. Ichimura (1998)
J. Biol. Chem
M Karandikar (2000)
Science's STKE http://stke.sciencemag. org/cgi/content/full/sigtrans
T Raabe (2002)
10.1128/MCB.19.11.7539
JSAP1, a Novel Jun N-Terminal Protein Kinase (JNK)-Binding Protein That Functions as a Scaffold Factor in the JNK Signaling Pathway
M. Ito (1999)
10.1073/PNAS.91.16.7762
Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module.
S. Marcus (1994)



This paper is referenced by
10.1111/J.1365-313X.2004.02229.X
Stress hormone-independent activation and nuclear translocation of mitogen-activated protein kinases in Arabidopsis thaliana during ozone exposure.
Reetta Ahlfors (2004)
10.3814/2008/143656
Analysis of the Arabidopsis Mitogen-Activated Protein Kinase Families: Organ Specificity and Transcriptional Regulation upon Water Stresses
K. Moustafa (2008)
10.1093/jxb/erx424
MAP kinases associate with high molecular weight multiprotein complexes
Carlton J. Bequette (2018)
Programmed cell death in plants resembles apoptosis of animals
C. Collazo (2006)
10.1007/978-90-481-3112-9_7
Protein Kinases and Phosphatases for Stress Signal Transduction in Plants
L. Chae (2009)
10.1094/MPMI-19-0711
Early signaling events induced by elicitors of plant defenses.
Angela Garcia-Brugger (2006)
Mitogen-Activated Protein (MAP) Kinases in Plant Metal Stress: Regulation and Responses in Comparison to Other Biotic
Kelly Opdenakker (2012)
10.1016/J.TPLANTS.2005.05.009
Emerging MAP kinase pathways in plant stress signalling.
H. Nakagami (2005)
10.3389/fpls.2015.00556
Mitogen-activated protein kinase cascades in Vitis vinifera
B. Çakır (2015)
MAPKinases in cadmium or copper-mediated oxidative stress in Arabidopsis thaliana
K. Opdenakker (2012)
10.1186/s12870-014-0219-1
Genome-wide identification and analysis of mitogen activated protein kinase kinase kinase gene family in grapevine (Vitis vinifera)
G. Wang (2014)
10.1186/1471-2229-12-103
Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana
M. Hashimoto (2012)
10.1007/s11103-008-9306-8
The genetic locus At1g73660 encodes a putative MAPKKK and negatively regulates salt tolerance in Arabidopsis
L. Gao (2008)
10.1111/j.1742-4658.2011.08056.x
Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress
L. Zhang (2011)
10.1111/j.1365-3040.2011.02387.x
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
10.15252/embj.201488582
GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis
Michael Wrzaczek (2015)
10.4161/psb.2.1.3885
The More We Know, the Less We Understand?
T. Mészáros (2007)
10.5423/PPJ.2010.26.2.101
Functions of MAPK Cascade Pathways in Plant Defense Signaling
Yong Hwa Cheong (2010)
10.1007/978-3-319-32423-4_3
Tolerance to Drought Stress in Plants: Unravelling the Signaling Networks
Karaba N. Nataraja (2016)
10.1016/j.biortech.2017.12.070
Structural basis for expanding the application of bioligand in metal bioremediation: A review.
V. Sharma (2018)
10.1271/bbb.68.1805
Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of Lotus japonicus
Natsuko Kinoshita (2004)
10.1016/J.SAJB.2011.09.004
Molecular cloning and expression characteristics of a novel MAPKKK gene, GhCTR1, from cotton (Gossypium hirsutum L.)
Feifei Yu (2012)
10.1104/pp.106.078295
Reactive Oxygen Species in Plant Cell Death1
F. Van Breusegem (2006)
10.1002/BIES.20493
Reactive oxygen species as signals that modulate plant stress responses and programmed cell death
T. Gechev (2006)
10.1007/BF02788882
Genomic analysis of MAP kinase cascades inArabidopsis defense responses
M. Cvetkovska (2007)
10.1186/1471-2229-13-121
Interaction between two rice mitogen activated protein kinases and its possible role in plant defense
A. Sheikh (2012)
10.1104/pp.112.210724
The MPK6-ERF6-ROS-Responsive cis-Acting Element7/GCC Box Complex Modulates Oxidative Gene Transcription and the Oxidative Response in Arabidopsis1[W][OA]
P. Wang (2013)
10.1093/jxb/eru092
Identification and functional analysis of mitogen-activated protein kinase kinase kinase (MAPKKK) genes in canola (Brassica napus L.)
Y. Sun (2014)
10.1007/s11240-015-0865-5
Cotton mitogen-activated protein kinase4 (GhMPK4) confers the transgenic Arabidopsis hypersensitivity to salt and osmotic stresses
Na-na Wang (2015)
10.1083/jcb.200409170
Hydrogen peroxide as a signal controlling plant programmed cell death
T. Gechev (2005)
10.1080/15216540500090793
Hydrogen Peroxide‐induced Cell Death in Arabidopsis: Transcriptional and Mutant Analysis Reveals a Role of an Oxoglutarate‐dependent Dioxygenase Gene in the Cell Death Process
T. Gechev (2005)
Molecular cloning and expression characteristics of a novel MAPKKK gene , GhCTR 1 , from cotton ( Gossypium hirsutum L . )
F. Yu (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar