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Photosystem I Is Indispensable For Photoautotrophic Growth, CO2 Fixation, And H2 Photoproduction InChlamydomonas Reinhardtii *

K. Redding, L. Cournac, I. Vassiliev, J. Golbeck, G. Peltier, J. Rochaix
Published 1999 · Biology, Medicine

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Certain Chlamydomonas reinhardtiimutants deficient in photosystem I due to defects in psaAmRNA maturation have been reported to be capable of CO2fixation, H2 photoevolution, and photoautotrophic growth (Greenbaum, E., Lee, J. W., Tevault, C. V., Blankinship, S. L., and Mets, L. J. (1995) Nature 376, 438–441 and Lee, J. W., Tevault, C. V., Owens, T. G.; Greenbaum, E. (1996) Science 273, 364–367). We have generated deletions of photosystem I core subunits in both wild type and these mutant strains and have analyzed their abilities to grow photoautotrophically, to fix CO2, and to photoevolve O2 or H2 (using mass spectrometry) as well as their photosystem I content (using immunological and spectroscopic analyses). We find no instance of a strain that can perform photosynthesis in the absence of photosystem I. The F8 strain harbored a small amount of photosystem I, and it could fix CO2 and grow slowly, but it lost these abilities after deletion of eitherpsaA or psaC; these activities could be restored to the F8-psaAΔ mutant by reintroduction ofpsaA. We observed limited O2photoevolution in mutants lacking photosystem I; use of18O2 indicated that this O2evolution is coupled to O2 uptake (i.e.respiration) rather than CO2 fixation or H2evolution. We conclude that the reported instances of CO2fixation, H2 photoevolution, and photoautotrophic growth of photosystem I-deficient mutants result from the presence of unrecognized photosystem I.
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