Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Assembly Of Vault-like Particles In Insect Cells Expressing Only The Major Vault Protein*
A. G. Stephen, S. Raval-Fernandes, T. Huynh, M. Torres, V. Kickhoefer, L. Rome
Published 2001 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Vaults are the largest (13 megadalton) cytoplasmic ribonucleoprotein particles known to exist in eukaryotic cells. They have a unique barrel-shaped structure with 8-fold symmetry. Although the precise function of vaults is unknown, their wide distribution and highly conserved morphology in eukaryotes suggests that their function is essential and that their structure must be important for their function. The 100-kDa major vault protein (MVP) constitutes ∼75% of the particle mass and is predicted to form the central barrel portion of the vault. To gain insight into the mechanisms for vault assembly, we have expressed rat MVP in the Sf9 insect cell line using a baculovirus vector. Our results show that the expression of the rat MVP alone can direct the formation of particles that have biochemical characteristics similar to endogenous rat vaults and display the distinct vault-like morphology when negatively stained and examined by electron microscopy. These particles are the first example of a single protein polymerizing into a non-spherically, non-cylindrically symmetrical structure. Understanding vault assembly will enable us to design agents that disrupt vault formation and hence aid in elucidating vault functionin vivo.
This paper references
Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry
N. Kedersha (1991)
Isolation and characterization of a novel ribonucleoprotein particle: large structures contain a single species of small RNA
N. Kedersha (1986)
Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes
N. Kedersha (1990)
Recombinant Major Vault Protein Is Targeted to Neuritic Tips of PC12 Cells
Christine E. Herrmann (1999)
Structure of the vault, a ubiquitous celular component.
L. B. Kong (1999)
cDNA cloning and disruption of the major vault protein alpha gene (mvpA) in Dictyostelium discoideum.
S. Vasu (1993)
The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle
V. Kickhoefer (2001)
RNA location and modeling of a WD40 repeat domain within the vault.
L. B. Kong (2000)
Baculovirus multigene expression vectors and their use for understanding the assembly process of architecturally complex virus particles.
P. Roy (1997)
Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III.
V. Kickhoefer (1993)
The sequence of a cDNA encoding the major vault protein from Rattus norvegicus.
V. Kickhoefer (1994)
Up‐regulation of vaults may be necessary but not sufficient for multidrug resistance
A. Siva (2001)
Dictyostelium Vaults: Disruption of the Major Proteins Reveals Growth and Morphological Defects and Uncovers a New Associated Protein (*)
S. Vasu (1995)
Evidence that vault ribonucleoprotein particles localize to the nuclear pore complex.
D. Chugani (1993)
Vaults and Telomerase Share a Common Subunit, TEP1*
V. Kickhoefer (1999)
The 193-Kd Vault Protein, Vparp, Is a Novel Poly(Adp-Ribose) Polymerase
V. Kickhoefer (1999)
Vaults Are Up-regulated in Multidrug-resistant Cancer Cell Lines*
V. Kickhoefer (1998)
Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells
D. Gheysen (1989)
Expression and self-assembly of empty virus-like particles of hepatitis E virus.
T. Li (1997)
Multidrug resistance and the lung resistance-related protein in human colon carcinoma SW-620 cells.
M. Kitazono (1999)
Self-assembly of the infectious bursal disease virus capsid protein, rVP2, expressed in insect cells and purification of immunogenic chimeric rVP2H particles by immobilized metal-ion affinity chromatography.
M. Wang (2000)
Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles.
R. Kirnbauer (1993)
This paper is referenced by
Beyond BLASTing: Tertiary and Quaternary Structure Analysis Helps Identify Major Vault Proteins
Toni K. Daly (2013)
Vault nanoparticles engineered with the protein transduction domain, TAT48, enhances cellular uptake.
J. Yang (2013)
Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer.
Cheng-Yu Lai (2009)
Epstein-barr virus-induced expression of a novel human vault RNA.
Constanze Nandy (2009)
Bioengineered Vaults: Self-Assembling Protein Shell–Lipophilic Core Nanoparticles for Drug Delivery
Daniel C. Buehler (2014)
Expression of Cell Vault Components MVP, TEP1 and vPARP in Cancerous Ovarian Tissues* Ekspresja składników krypt komórkowych MVP, TEP1 oraz vPARP w tkankach nowotworowych z jajnika
Witold Szaflarski (2011)
The formation of vault-tubes: a dynamic interaction between vaults and vault PARP
A. van Zon (2003)
Synthesis and assembly of human vault particles in yeast
M. Wang (2018)
Expression profiles of vault components MVP, TEP1 and vPARP and their correlation to other multidrug resistance proteins in ovarian cancer.
Witold Szaflarski (2013)
Vault Poly(ADP-Ribose) Polymerase Is Associated with Mammalian Telomerase and Is Dispensable for Telomerase Function and Vault Structure In Vivo
Y. Liu (2004)
Investigating Antibody Access to Adsorbed Protein Nanocapsule Interiors Using the Quartz Crystal Microbalance and Surface Plasmon Resonance
M. Yu (2006)
Vault particles: a new generation of delivery nanodevices.
A. Casañas (2012)
Nuclear localization of the major vault protein in U373 cells
M. Slesina (2005)
Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level
Nicholas E. Dickenson (2008)
Vault Nanoparticles: Chemical Modifications for Imaging and Enhanced Delivery.
N. Benner (2017)
Targeted vault nanoparticles engineered with an endosomolytic peptide deliver biomolecules to the cytoplasm.
Muri Han (2011)
Artificial bioparticle and method for manufacturing same
秀明 田中 (2013)
Disruption of the murine major vault protein (MVP/LRP) gene does not induce hypersensitivity to cytostatics.
M. Mossink (2002)
The yeast Coq4 polypeptide organizes a mitochondrial protein complex essential for coenzyme Q biosynthesis.
B. Marbois (2009)
Correction: Recruitment of the Major Vault Protein by InlK: A Listeria monocytogenes Strategy to Avoid Autophagy
L. Dortet (2011)
The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle
Michael J Poderycki (2005)
Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange.
J. Yang (2010)
A Protective Vaccine against Chlamydia Genital Infection Using Vault Nanoparticles without an Added Adjuvant
Janina Jiang (2017)
Nanocapsules: the weapons for novel drug delivery systems.
Pavankumar Kothamasu (2012)
Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope.
Ye Zhu (2015)
Engineering protein self-assembling in protein-based nanomedicines for drug delivery and gene therapy
N. Ferrer-Miralles (2015)
EFFECT OF PARTIAL POLY(ADP-RIBOSE) GLYCOHYDROLASE GENE DELETION ON CELLULAR RESPONSES TO GENOTOXIC STRESS
H. Gao (2006)
Bioengineering Strategies for Protein-Based Nanoparticles
D. Diaz (2018)
Smart vaults: thermally-responsive protein nanocapsules.
Nicholas M. Matsumoto (2013)
Molecular cloning and characterization of major vault protein of Echinococcus multilocularis.
A. Goto (2013)
Mechanical stability and reversible fracture of vault particles.
A. Llauró (2014)See more