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The Cytoplasmic Tail Of α1,3-Galactosyltransferase Inhibits Golgi Localization Of The Full-length Enzyme*
J. Milland, S. Russell, H. C. Dodson, I. Mckenzie, M. Sandrin
Published 2002 · Biology, Medicine
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It is currently under debate whether the mechanism of Golgi retention of different glycosyltransferases is determined by sequences in the transmembrane, luminal, or cytoplasmic domains or a combination of these domains. We have shown that the cytoplasmic domains of α1,3-galactosyltransferase (GT) and α1,2-fucosyltransferase (FT) are involved in Golgi localization. Here we show that the cytoplasmic tails of GT and FT are sufficient to confer specific Golgi localization. Further, we show that the expression of only the cytoplasmic tail of GT can lead to displacement or inhibition of binding of the whole transferase and that cells expressing the cytoplasmic tail of GT were not able to express full-length GT or its product, Galα1,3Gal. Thus, the presence of the cytoplasmic tail prevented the localization and function of full-length GT, suggesting a possible specific Golgi binding site for GT. The effect was not altered by the inclusion of the transmembrane domain. Although the transmembrane domain may act as an anchor, these data show that, for GT, only the cytoplasmic tail is involved in specific localization to the Golgi.
This paper references
A signal for Golgi retention in the bunyavirus G1 glycoprotein.
Y. Matsuoka (1994)
Progress in unraveling pathways of Golgi traffic.
M. Farquhar (1985)
Characterization of cDNA clones for porcine α(l,3)galactosyl transferase: The enzyme generating the Galα(l,3)Gal epitope
M. Sandrin (1994)
Truncations of the C-terminal cytoplasmic domain of MG160, a medial Golgi sialoglycoprotein, result in its partial transport to the plasma membrane and filopodia.
J. Gonatas (1998)
Mammalian glycosyltransferases: genomic organization and protein structure.
D. Joziasse (1992)
Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha 1,2-mannosyltransferase to a medial-Golgi compartment
M. Lussier (1995)
An investigation of the role of transmembrane domains in Golgi protein retention.
S. Munro (1995)
Modification of the cytoplasmic domain affects the subcellular localization of Golgi glycosyl-transferases.
W. Yang (1996)
Sequences within and adjacent to the transmembrane segment of alpha-2,6-sialyltransferase specify Golgi retention.
S. Munro (1991)
Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum.
T. Fujiwara (1988)
A comparison of the transmembrane domains of Golgi and plasma membrane proteins.
S. Munro (1995)
The Cytoplasmic Tail of α1,2-Fucosyltransferase Contains a Sequence for Golgi Localization*
J. Milland (2001)
Implications of the SNARE hypothesis for intracellular membrane topology and dynamics
J. Rothman (1994)
A retention signal necessary and sufficient for Golgi localization maps to the cytoplasmic tail of a Bunyaviridae (Uukuniemi virus) membrane glycoprotein.
A. Andersson (1997)
Specific sequences in the signal anchor of the beta-galactoside alpha-2,6-sialyltransferase are not essential for Golgi localization. Membrane flanking sequences may specify Golgi retention.
R. Y. Dahdal (1993)
The role of the membrane-spanning domain and stalk region of N-acetylglucosaminyltransferase I in retention, kin recognition and structural maintenance of the Golgi apparatus in HeLa cells.
T. Nilsson (1996)
Isolation and characterization of cDNA clones for mouse Ly-9.
M. Sandrin (1992)
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
G. Evan (1985)
The Curious Status of the Golgi Apparatus
B. Glick (1998)
Procollagen Traverses the Golgi Stack without Leaving the Lumen of Cisternae Evidence for Cisternal Maturation
L. Bonfanti (1998)
A C-terminally-anchored Golgi protein is inserted into the endoplasmic reticulum and then transported to the Golgi apparatus.
A. Linstedt (1995)
The cytoplasmic tail of mouse hepatitis virus M protein is essential but not sufficient for its retention in the Golgi complex.
J. K. Locker (1994)
Trafficking and localisation of resident Golgi glycosylation enzymes.
A. Opat (2001)
The isolation of cDNA clones for CD48
HilaryA. Vaughan (2004)
Compartmental organization of the golgi stack
W. Dunphy (1985)
The retention signal for soluble proteins of the endoplasmic reticulum.
H. Pelham (1990)
Distinct Roles for the Cytoplasmic Tail Sequences of Emp24p and Erv25p in Transport between the Endoplasmic Reticulum and Golgi Complex*
W. Belden (2001)
Sequencing of a new HLA-A*32 subtype (A*3202)
E. Zino (1996)
Targeting of a Short Peptide Derived from the Cytoplasmic Tail of the G1 Membrane Glycoprotein of Uukuniemi Virus (Bunyaviridae) to the Golgi Complex
A. Andersson (1998)
An Essential Cytoplasmic Domain for the Golgi Localization of Coiled-coil Proteins with a COOH-terminal Membrane Anchor*
Y. Misumi (2001)
EXPRESSION OF A CYTOPLASMICALLY EPITOPE‐TAGGED, HUMAN GOLGI GLYCOSYLTRANSFERASE IN HOMOLOGOUS CELLS RESULTS IN MISLOCALIZATION OF MULTIPLE GOLGI PROTEINS
W. Yang (1997)
Beta 1,4-galactosyltransferase: a short NH2-terminal fragment that includes the cytoplasmic and transmembrane domain is sufficient for Golgi retention.
R. Russo (1992)
Assembly of asparagine-linked oligosaccharides.
R. Kornfeld (1985)
The Cytoplasmic, Transmembrane, and Stem Regions of Glycosyltransferases Specify Their in Vivo Functional Sublocalization and Stability in the Golgi*
E. Grabenhorst (1999)
T. Nilsson (1993)
Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum
M. Lewis (1992)
Recruitment to Golgi membranes of ADP‐ribosylation factor 1 is mediated by the cytoplasmic domain of p23
D. Gommel (2001)
The signal for Golgi retention of bovine beta 1,4-galactosyltransferase is in the transmembrane domain.
R. Teasdale (1992)
A cisternal maturation mechanism can explain the asymmetry of the Golgi stack
B. Glick (1997)
Sequence of a second human KDEL receptor.
M. Lewis (1992)
Targeting of proteins to the Golgi apparatus
P. Gleeson (2004)
P. A. Gleeson (1998)
Switching Amino-terminal Cytoplasmic Domains of α(1,2)Fucosyltransferase and α(1,3)Galactosyltransferase Alters the Expression of H Substance and Galα(1,3)Gal*
N. Osman (1996)
The membrane spanning domain of beta‐1,4‐galactosyltransferase specifies trans Golgi localization.
T. Nilsson (1991)
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C. Götting (2007)
Secretion and fluid transport mechanisms in the mammary gland
J. Monks (2013)
Cloning and Recombinant Expression of Active Full-length Xylosyltransferase I (XT-I) and Characterization of Subcellular Localization of XT-I and XT-II*
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Functional involvement of TMF/ARA160 in Rab6-dependent retrograde membrane traffic.
Junko Yamane (2007)
Multiple Signals Are Required for α2,6-Sialyltransferase (ST6Gal I) Oligomerization and Golgi Localization*
F. H. Fenteany (2005)
Deletion of the cytoplasmic domain of human alpha3/4 fucosyltransferase III causes the shift of the enzyme to early Golgi compartments.
V. L. Sousa (2004)
Differential timing of translocation of HIV-1 subtype B and C Vpu to the ER/Golgi an plasma membrane compartments
C. M. Bell (2010)
Missense mutations outside the catalytic domain of the ABO glycosyltransferase can cause weak blood group A and B phenotypes
A. Seltsam (2005)
Glycosylation of glycolipids in the Golgi complex
H. Maccioni (2007)
Subcellular Localization of Mammalian Type II Membrane Proteins
R. Aturaliya (2006)
ABO blood group and related antigens, natural antibodies and transplantation.
J. Milland (2006)
Association of beta-1,3-N-acetylglucosaminyltransferase 1 and beta-1,4-galactosyltransferase 1, trans-Golgi enzymes involved in coupled poly-N-acetyllactosamine synthesis.
Peter L Lee (2009)
Golgi localization of glycosyltransferases requires Gpp74p in Schizosaccharomyces pombe.
T. Ohashi (2020)
Triple arginines in the cytoplasmic tail of endomannosidase are not essential for type II membrane topology and Golgi localization
J. Stehli (2008)
Fucosyltransferase IX: characterization and biological role
C. Brito (2007)
Translation of genome to glycome: role of the Golgi apparatus
Prathyush Pothukuchi (2019)
The cytoplasmic and transmembrane domains of secretor type α1,2fucosyltransferase confer atypical cellular localisation
D. Christiansen (2009)
Synthetic glycobiology: Exploits in the Golgi compartment.
Jennifer L Czlapinski (2006)
α1,3‐Galactosyltransferase knockout pigs are available for xenotransplantation: Are glycosyltransferases still relevant?
J. Milland (2005)
The Molecular Basis for Galα(1,3)Gal Expression in Animals with a Deletion of the α1,3Galactosyltransferase Gene12
J. Milland (2006)
Importance of Cys, Gln, and Tyr from the Transmembrane Domain of Human α3/4 Fucosyltransferase III for Its Localization and Sorting in the Golgi of Baby Hamster Kidney Cells*
V. L. Sousa (2003)
Analysis of Binding Events and Diffusion in Living Cells
M. Elsner (2007)
Intracellular sorting and transport of proteins.
C. van Vliet (2003)
Deregulation of the carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11) gene in a B-cell chronic lymphocytic leukemia with a t(12;14)(q23;q32)
H. Schmidt (2004)
The extended cytoplasmic tail of the human B4GALNT2 is critical for its Golgi targeting and post‐Golgi sorting
Sophie Groux-Degroote (2018)
Regulating cell surface glycosylation by small molecule control of enzyme localization.
Jennifer J. Kohler (2003)
Golgi glycosylation enzymes
E. G. Berger (2008)
Modulation of GalT1 and SialT1 Sub-Golgi Localization by SialT2 Expression Reveals an Organellar Level of Glycolipid Synthesis Control*
A. Uliana (2006)
Ligand Binding Determines Whether CD46 Is Internalized by Clathrin-coated Pits or Macropinocytosis*
B. Crimeen-Irwin (2003)
Plant N-Glycan Processing Enzymes Employ Different Targeting Mechanisms for Their Spatial Arrangement along the Secretory Pathway[W][OA]
C. Saint-Jore-Dupas (2006)
Transcription starting from an alternative promoter leads to the expression of the human ABO histo‐blood group antigen
Y. Hata (2003)
Regulating cell surface glycosylation with a small-molecule switch.
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