Online citations, reference lists, and bibliographies.
← Back to Search

Rapid Turbidimetric Determination Of Lipase Activity In Biological Fluids

P. Arzoglou, A. Tavridou, C. Balaska
Published 1989 · Chemistry

Save to my Library
Download PDF
Analyze on Scholarcy
Abstract An improved turbidimetric technique for the determination of lipase activity is presented. A stable and reproducible triolein emulsion obtained with the use of isopropanol is suitable substrate for the lipase activities present in human serum and pancreatic juice. The within-run precision CV is 5.2 % whereas the day-to-day precision CV is 7.3 % for enzyme activities near 400 U/l. Linear response is obtained for lipase activities ranging from 60 to 780 U/l. the detection lower limit is 18 U/l. the use of optimal colipase, sodium taurodeoxycholate and calcium chloride concentrations offer the system increased specificity and analytical sensitivity.
This paper references
An enzymatic method for calibration of serum lipase assays.
G. Hoffmann (1986)
Measurement of lipase activity by a differential pH technique.
F. Ceriotti (1985)
Detection of colipase in serum and urine of pancreatitis patients.
W. Junge (1982)
Nephelometric determination of pancreatic enzymes. II. Lipase.
L. Zinterhofer (1973)
Lipase assay in duodenal juice using a conductimetric method.
C. Ballot (1984)
Assays of serum lipase: analytical and clinical considerations.
J. Lott (1986)
A rapid and sensitive turbidimetric method for serum lipase based upon differences between the lipases of normal and pancreatitis serum.
W. Vogel (1963)
Assay of human pancreatic lipase in biological fluids using a non-competitive enzyme immunoassay.
J. Carrère (1986)
Characterization and elimination of a factor in serum that interferes with turbidimetry and nephelometry of lipase.
H. Kannisto (1983)
Differentiating two forms of plasma lipase by use of media with different ionic strengths.
P. Arzoglou (1984)
Fluorometric assay for pancreatic lipase.
R. Salvayre (1986)
Detection and correction of falsely elevated lipase values as measured nephelometrically.
J. L. Pope (1977)
Proposed standard method for measuring lipase activity in serum by a continuous sampling technique.
N. Tietz (1973)
Simplified turbidimetric assay for lipase activity.
Z. Shihabi (1971)
Advances in clinical enzymology
E. Schmidt (1979)
Plasma lipase properties as related to pancreatic condition.
P. Arzoglou (1986)
Conditions for assay of pancreatic lipase from human plasma using a nephelometric technique.
P. Arzoglou (1982)
A novel and simple colorimetric assay for human serum lipase.
S. Kurooka (1977)
Turbidimetric measurement of lipase activity--problems and some solutions.
N. Tietz (1987)
A comparison of currently used serum lipase and amylase procedures in the serial detection of enzyme elevations in acute pancreatitis.
J. A. Hathaway (1983)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar