Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Inhibition Of Tumor Promoter-induced Hydrogen Peroxide Formation In Vitro And In Vivo By Genistein.
H. Wei, L. Wei, K. Frenkel, R. Bowen, S. Barnes
Published 1993 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Here we report that genistein, a soybean isoflavone, strongly inhibits tumor promoter-induced H2O2 formation both in vivo and in vitro. Genistein suppressed H2O2 production by 12-O-tetradecanoylphorbol-13-acetate- (TPA) stimulated human polymorphonuclear leukocytes (PMNs) and HL-60 cells in a dose-dependent manner over the concentration range 1-150 microM. Human PMNs were more sensitive to the inhibitory effect of genistein than HL-60 cells (50% inhibitory concentration 14.8 and 30.2 microM, respectively). In addition, genistein moderately inhibited superoxide anion formation by HL-60 cells and scavenged exogenously added H2O2 under the same conditions as in cell culture. However, the H2O2-scavenging effect of genistein was about 50% lower than its inhibition of cell-derived H2O2 formation at all concentrations. In the CD-1 mouse skin model, genistein strongly inhibited TPA-induced oxidant formation, edema, and PMN infiltration in mouse skin. Inhibition of TPA-mediated H2O2 in vivo may result from decreased cell-derived H2O2 formation, scavenging of H2O2 produced, and/or suppression of PMN infiltration into the dermis. The antioxidant properties of genistein may be responsible for its anticarcinogenic effects, and the dietary availability of genistein makes it a promising candidate for the prevention of human cancers.
This paper references
T. Hunter (1985)
Inhibition of PDGF-induced c-jun and c-fos expression by a tyrosine protein kinase inhibitor.
J. Zwiller (1991)
Inflammation and cancer: role of phagocyte-generated oxidants in carcinogenesis
S. A. Weitzman (1990)
Lactoferrin enhances hydroxyl radical production by human neutrophils, neutrophil particulate fractions, and an enzymatic generating system.
D. Ambruso (1981)
The role of soy products in reducing risk of cancer.
M. Messina (1991)
Development and application of a high‐performance liquid chromatographic method for the analysis of phytoestrogens
A. E. Jones (1989)
12-O-tetradecanoylphorbol-13-acetate-dependent induction of xanthine dehydrogenase and conversion to xanthine oxidase in murine epidermis.
J. Reiners (1987)
Antioxidants isolated from Fermented Soybeans (Tempeh)
P. György (1964)
Tyrosine phosphorylation and its possible role in superoxide production by human neutrophils stimulated with FMLP and IgG.
T. Kusunoki (1992)
Induction of mammalian topoisomerase II dependent DNA cleavage by nonintercalative flavonoids, genistein and orobol.
Yamashita Yoshinori (1990)
Mutagenicities of 61 flavonoids and 11 related compounds.
M. Nagao (1981)
Mechanisms of action in NIH-3T3 cells of genistein, an inhibitor of EGF receptor tyrosine kinase activity.
C. Linassier (1990)
Genistein, a specific inhibitor of tyrosine-specific protein kinases.
T. Akiyama (1987)
 Superoxide production
P. O'brien (1984)
Lack of mutagenicity of some phytoestrogens in the salmonella/mammalian microsome assay.
R. Bartholomew (1980)
Radiation-like modification of bases in DNA exposed to tumor promoter-activated polymorphonuclear leukocytes.
K. Frenkel (1986)
Genistein inhibition of the growth of human breast cancer cells: independence from estrogen receptors and the multi-drug resistance gene.
G. Peterson (1991)
Uncoupling and inhibition of the respiratory chain in rat liver mitochondria by some naturally occurring estrogens and their metabolites
T. Lundh (1991)
Inhibition of in vitro microsomal lipid peroxidation by isoflavonoids.
H. Jha (1985)
Inhibition of the mitochondrial NADH-oxidase (NADH-coenzyme Q oxido-reductase) enzyme system by flavonoids: a structure-activity study.
W. F. Hodnick (1987)
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
C. Crews (1992)
Suppression of tumor promoter-induced oxidative events and DNA damage in vivo by sarcophytol A: a possible mechanism of antipromotion.
H. Wei (1992)
Induction of in vitro differentiation of mouse embryonal carcinoma (F9) cells by inhibitors of topoisomerases.
K. Kondo (1991)
Inhibition of the mutagenicity of bay-region diol-epoxides of polycyclic aromatic hydrocarbons by phenolic plant flavonoids.
M. Huang (1983)
Inhibitory effects of the tyrosine kinase inhibitor genistein on mammalian DNA topoisomerase II.
J. Markovits (1989)
Oxidation of DNA bases by tumor promoter-activated processes.
K. Frenkel (1989)
MAP kinases: charting the regulatory pathways.
S. Pelech (1992)
Effects of synthetic and naturally occurring flavonoids on benzo[a]pyrene metabolism by hepatic microsomes prepared from rats treated with cytochrome P-450 inducers.
Y. H. Chae (1991)
Effect of genistein on topoisomerase activity and on the growth of [Val 12]Ha-ras-transformed NIH 3T3 cells.
A. Okura (1988)
Protein phosphorylation associated with the stimulation of neutrophils. Modulation of superoxide production by protein kinase C and calcium
P. Heyworth (1990)
Nonsteroidal estrogens of dietary origin: possible roles in hormone-dependent disease.
K. Setchell (1984)
Carcinogen-mediated oxidant formation and oxidative DNA damage.
K. Frenkel (1992)
Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds.
S. Collins (1978)
In vivo formation of oxidized DNA bases in tumor promoter-treated mouse skin.
H. Wei (1991)
Phenolic Antioxidants of Soy Protein Hydrolyzates
D. Pratt (1982)
Soybeans inhibit mammary tumors in models of breast cancer.
S. Barnes (1990)
Induction of in vitro differentiation of mouse erythroleukemia cells by genistein, an inhibitor of tyrosine protein kinases.
T. Watanabe (1991)
Induction of differentiation and DNA strand breakage in human HL-60 and K-562 leukemia cells by genistein.
A. Constantinou (1990)
This paper is referenced by
Revisión de conjunto Fitoestrógenos: conocimientos básicos y utilidad clínica
C. Castelo-Branco (2016)
Isolation and Identification of Acetylenic Spiroketal Enol Ethers from Artemisia lactiflora as Inhibitors of Superoxide Generation Induced by a Tumor Promoter in Differentiated HL-60 Cells
Y. Nakamura (1998)
Estrogenic and antiproliferative properties of genistein and other flavonoids in human breast cancer cells in vitro.
D. Zava (1997)
Effects of genistein, a soybean-derived isoflavone, on proliferation and differentiation of B16-BL6 mouse melanoma cells.
C. Yan (1999)
Phytoestrogens: epidemiology and a possible role in cancer protection.
H. Adlercreutz (1995)
Inhibition of ultraviolet light-induced oxidative events in the skin and internal organs of hairless mice by isoflavone genistein.
H. Wei (2002)
Genistein Suppresses Spontaneous Oviduct Tumorigenesis in Quail
K. Şahin (2009)
Genotoxicity and Carcinogenicity Studies of Soy Isoflavones
R. Misra (2002)
Isolation and Partial Characterization of Isoflavone Transforming Lactobacillus plantarum YS712 for Potential Probiotic Use
Yoon-Hee Cho (2009)
Nutritional approaches to the prevention of prostate cancer progression.
I. Yip (1996)
Phytoestrogens in Clinical Practice
S. Davis (1998)
Endometrial cancer: hormonal factors, the perimenopausal "window of risk," and isoflavones.
G. Hale (2002)
고지혈증 흰쥐에서 비배당체 이소플라본 고함유 대두분말의 혈청 지질 대사 영향과 항산화효과
Obesity-Related Epigenetic Changes After Bariatric Surgery
A. Izquierdo (2019)
Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice.
E. Kirk (1998)
Antioxidant activities of isoflavones and their biological metabolites in a liposomal system.
A. Arora (1998)
Protective effect of L-carnosine against 12-O-tetradecanoylphorbol-13-acetate- or hydrogen peroxide-induced apoptosis on v-myc transformed rat liver epithelial cells.
Kyung Sun Kang (2002)
NUTRACÈUTICS: IMPLICACIÓ TERAPÈUTICA EN CAQUÈXIA CANCEROSA I DIABETIS
D. D. Biologia (2009)
Soybeans as an Alternative to Hormone Replacement Therapy
M. Chavez (2001)
Changes in Physically-Chemical Parameters of Latvian Cranberries During Storage
Karina Ruse (2013)
ESR and cell culture studies on free radical-scavenging and antioxidant activities of isoflavonoids.
Q. Guo (2002)
Effects of Soybean. Cheonggukjang and Doenjang on Serum Cholesterol Level and Weight Reduction in Rats Fed a High-Fat/High-Cholesterol Diet
J. Lee (2011)
Mouse Models of Epigenetic Inheritance
A. Bernal (2011)
Soy isoflavonoids and cancer -- metabolism at the target site.
B. Boersma (2001)
Chemoprevention of Skin Cancer through Natural Agents
S. Gupta (2001)
Soy protein, isoflavonoids, and risk of developing coronary heart disease
A. Lichtenstein (1999)
Suppression by citrus auraptene of phorbol ester-and endotoxin-induced inflammatory responses: role of attenuation of leukocyte activation.
A. Murakami (2000)
Physiological effects of phyto-oestrogens in relation to cancer and other human health risks.
A. Cassidy (1996)
Human and Clinical Nutrition A Diet High in Wheat Fiber Decreases the Bioavailability of Soybean Isoflavones in a Single Meal Fed to Women 1 ' 2
B. W. Tew (2006)
CHAPTER 32 – Soy Isoflavones
S. Barnes (2006)
Are Soy-Milk Products Viable Alternatives to Cow’s Milk?
J. Woodside (2016)
Antioxidants and their implication in oral health and general health
L. Swapna (2014)See more