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Nanoethosomal Formulation Of Gammaoryzanol For Skin-aging Protection And Wrinkle Improvement: A Histopathological Study

S. Heydari, S. Ghanbarzadeh, Behzad Anoush, M. Ranjkesh, Y. Javadzadeh, M. Kouhsoltani, H. Hamishehkar
Published 2017 · Chemistry, Medicine

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Abstract Background: Free radical scavengers and antioxidants, with the main focus on enhanced targeting to the skin layers, can provide protection against skin ageing. Objective: The aim of the present study was to prepare nanoethosomal formulation of gammaoryzanol (GO), a water insoluble antioxidant, for its dermal delivery to prevent skin aging. Methods: Nanoethosomal formulation was prepared by a modified ethanol injection method and characterized by using laser light scattering, scanning electronic microscope (SEM) and X-ray diffraction (XRD) techniques. The effects of formulation parameters on nanoparticle size, encapsulation efficiency percent (EE%) and loading capacity percent (LC%) were investigated. Antioxidant activity of GO-loaded formulation was investigated in vitro using normal African green monkey kidney fibroblast cells (Vero). The effect of control and GO-loaded nanoethosomal formulation on superoxide dismutase (SOD) and malondialdehyde (MDA) content of rat skin was also probed. Furthermore, the effect of GO-loaded nanoethosomes on skin wrinkle improvement was studied by dermoscopic and histological examination on healthy humans and UV-irradiated rats, respectively. Results: The optimized nanoethosomal formulation showed promising characteristics including narrow size distribution 0.17 ± 0.02, mean diameter of 98.9 ± 0.05 nm, EE% of 97.12 ± 3.62%, LC% of 13.87 ± 1.36% and zeta potential value of –15.1 ± 0.9 mV. The XRD results confirmed uniform drug dispersion in the nanoethosomes structure. In vitro and in vivo antioxidant studies confirmed the superior antioxidant effect of GO-loaded nanoethosomal formulation compared with control groups (blank nanoethosomes and GO suspension). Conclusions: Nanoethosomes was a promising carrier for dermal delivery of GO and consequently had superior anti-aging effect.
This paper references
10.1111/srt.12143
Skin texture aging trend analysis using dermoscopy images
Yoon-Hwa Choi (2014)
10.1021/la901565q
Aqueous-core lipid nanocapsules for encapsulating fragile hydrophilic and/or lipophilic molecules.
N. Anton (2009)
10.1016/j.colsurfb.2015.10.041
Enhanced stability and dermal delivery of hydroquinone using solid lipid nanoparticles.
S. Ghanbarzadeh (2015)
10.1016/S0378-6080(10)32014-9
Dermatological drugs, topical agents, and cosmetics
I. Duarte (2010)
10.1016/S1773-2247(13)50089-X
Formulation and Evaluation of Piroxicam Transferosomal Gel: An Approach for Penetration Enhancement
S. Ghanbarzadeh (2013)
10.2174/1381612821666150901095838
(Phospho)lipid-based Nanosystems for Skin Administration.
Ž. Vanić (2015)
10.1016/J.FOODCHEM.2008.04.031
Antioxidant components and properties of five long-grained rice bran extracts from commercial available cultivars in Thailand
C. Chotimarkorn (2008)
10.3109/03639045.2014.980426
Histopathological evaluation of caffeine-loaded solid lipid nanoparticles in efficient treatment of cellulite
H. Hamishehkar (2015)
Evaluation of different methods to produce nanoparticle containing gammaoryzanol for potential use in food fortification
S. Ghaderi (2014)
10.1155/2015/709628
Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity
Saima Jadoon (2015)
10.1007/s00403-009-1001-3
Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms
Joi A. Nichols (2009)
10.3390/md8041189
Anti-photoaging and Photoprotective Compounds Derived from Marine Organisms
Ramjee Pallela (2010)
10.1159/000360092
Reactive Molecule Species and Antioxidative Mechanisms in Normal Skin and Skin Aging
U. Wölfle (2014)
10.1074/jbc.M004392200
l-Ascorbic Acid Potentiates Endothelial Nitric Oxide Synthesis via a Chemical Stabilization of Tetrahydrobiopterin*
R. Heller (2001)
10.1159/000305548
Differential Effects of Low-Dose and High-Dose Beta-Carotene Supplementation on the Signs of Photoaging and Type I Procollagen Gene Expression in Human Skin in vivo
Soyun Cho (2010)
10.1155/2013/616810
Enhanced Transdermal Delivery of Diclofenac Sodium via Conventional Liposomes, Ethosomes, and Transfersomes
S. Ghanbarzadeh (2013)
10.1111/j.1600-0625.2012.01451.x
In vivo quantification of epidermis pigmentation and dermis papilla density with reflectance confocal microscopy: variations with age and skin phototype
S. Lagarrigue (2012)
10.1517/17425247.2012.666968
Liposomes in cosmeceutics
Yahya Rahimpour (2012)
10.1016/j.jaad.2011.08.021
Skin aging: in vivo microscopic assessment of epidermal and dermal changes by means of confocal microscopy.
C. Longo (2013)
10.1089/rej.2009.0906
Inhibitory effect of encapsulated curcumin on ultraviolet-induced photoaging in mice.
Rumjhum Agrawal (2010)
10.3109/03639045.2013.817416
Enhanced antioxidant effect of trans-resveratrol: potential of binary systems with polyethylene glycol and cyclodextrin
Josè Ramon Moyano-Mendez (2014)
10.1155/2013/930164
Skin Photoaging and the Role of Antioxidants in Its Prevention
Ruža Pandel (2013)
10.1016/J.FOODCHEM.2010.08.068
Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells
A. Mujić (2011)
10.1016/S0168-3659(99)00222-9
Ethosomes - novel vesicular carriers for enhanced delivery: characterization and skin penetration properties.
E. Touitou (2000)
10.2174/1381612821666150428145216
Nanocarriers and their Actions to Improve Skin Permeability and Transdermal Drug Delivery.
N. Khan (2015)
10.5681/apb.2014.081
Formulation of gammaoryzanol-loaded nanoparticles for potential application in fortifying food products.
S. Ghaderi (2014)
10.1111/j.1365-4632.2005.02079.x
Bathing in a magnesium‐rich Dead Sea salt solution improves skin barrier function, enhances skin hydration, and reduces inflammation in atopic dry skin
E. Proksch (2005)
10.1016/j.phymed.2011.10.003
Bioactive compounds from natural resources against skin aging.
P. Mukherjee (2011)
Histology image of right (a) and left (b) wrists of volunteer after 60 days of gammaoryzanol (GO)-loaded nanoethosomes and GO dispersion gels administration
Skin aging, antioxidants and free radicals
B. Poljsak (2012)
10.2147/IJN.S34921
A comparative evaluation of coenzyme Q10-loaded liposomes and solid lipid nanoparticles as dermal antioxidant carriers
E. Gokce (2012)
10.15171/apb.2016.006
The Effect of Particle Size on the Deposition of Solid Lipid Nanoparticles in Different Skin Layers: A Histological Study.
Zahra Mardhiah Adib (2016)
10.15171/APB.2016.06
The Effect of Particle Size on the Deposition of Solid Lipid Nanoparticles in Different Skin Layers: A Histological Study.
Z. MardhiahAdib (2016)
10.1016/j.ejpb.2010.11.003
Potentials of new nanocarriers for dermal and transdermal drug delivery.
R. Neubert (2011)
10.1038/SJ.JID.5700340
Oxidative stress in the pathogenesis of skin disease.
D. Bickers (2006)
10.1016/j.jdermsci.2010.03.003
Role of antioxidants in the skin: anti-aging effects.
H. Masaki (2010)
GAMMA ORYZANOL BEARING NANOLIPOSOME PRODUCED BY MODIFIED THERMAL METHOD: THERMAL PROPERTY, ENCAPSULATION EFFICIENCY, OSCILLATORY RHEOMETRY
Mohammad Hassani Zahra (2013)
10.1016/S0047-6374(01)00425-0
Molecular mechanisms of skin ageing
Gail Jenkins (2002)
10.15171/apb.2015.074
Nanoethosomes for Dermal Delivery of Lidocaine.
Soraya Babaie (2015)
10.1016/j.ijpharm.2013.04.038
Effect of antioxidant properties of lecithin emulsifier on oxidative stability of encapsulated bioactive compounds.
Yuanjie Pan (2013)
10.1016/S0378-6080(05)80436-2
14 Dermatological drugs, topical agents, and cosmetics
S. Knowles (2005)
Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L
L Sun (2011)
10.1111/j.1473-2165.2008.00354.x
Anti‐aging properties of resveratrol: review and report of a potent new antioxidant skin care formulation
R. Baxter (2008)
Differential effects of lowdose and high-dose beta-carotene supplementation on the signs of photoaging and type I procollagen gene expression in human skin in vivo
S Cho (2010)
10.1016/j.phymed.2008.02.014
The effect of trans-ferulic acid and gamma-oryzanol on ethanol-induced liver injury in C57BL mouse.
Chatchawan Chotimarkorn (2008)
10.4314/TJPR.V5I1.14634
Nanoparticles - A Review
V. Mohanraj (2007)
10.1046/J.1523-1747.2003.12021.X
Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse.
S. Inomata (2003)
10.1016/j.fct.2011.07.042
Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves.
L. Sun (2011)
Sunscreen formulations containing rice bran or soybean oil: rheological properties, spreadability and in vitro sun protection factor
L. A. Rigo (2011)
10.1155/2015/983484
Antioxidant Activity, Antitumor Effect, and Antiaging Property of Proanthocyanidins Extracted from Kunlun Chrysanthemum Flowers
S. Jing (2015)
10.3109/03639045.2015.1062896
Histological assessment of follicular delivery of flutamide by solid lipid nanoparticles: potential tool for the treatment of androgenic alopecia
H. Hamishehkar (2016)
Evaluation of Different Methods for Preparing Nanoparticle Containing Gammaoryzanol for Potential Use in Food Fortification
S. Ghaderi (2015)
10.1515/plm-2015-0023
Experiences on the influence of different behaviors on antioxidants and reactive oxygen species in the human skin
J. Lademann (2015)
10.1016/j.ijpharm.2012.04.066
Emerging trends in the stabilization of amorphous drugs.
R. Laitinen (2013)



This paper is referenced by
10.3390/molecules24061016
Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice
R. Yi (2019)
10.1016/j.ijbiomac.2020.08.079
Polycaprolactone nanofiber coated with chitosan and Gamma oryzanol functionalized as a novel wound dressing for healing infected wounds.
Hesaam Hajilou (2020)
10.1016/j.jddst.2020.101548
Angiogenesis and collagen promoting nutraceutical-loaded nanovesicles for wound healing
Saed Aldalaen (2020)
10.1080/14764172.2017.1358451
Vesicles: Potential nano carriers for the delivery of skin cosmetics
Alka Lohani (2017)
Redox Regulation in Animals for Slowing Ageing
Biswaranjan Paital (2017)
10.1080/08982104.2018.1517160
Transdermal applications of ethosomes – a detailed review
Nidhi Nainwal (2019)
10.1016/J.JDDST.2018.01.003
A comparative study on the potentials of nanoliposomes and nanoethosomes for Fluconazole delivery
Gashin Zandi (2018)
10.1080/17425247.2020.1693998
A brief overview on nano-sized materials used in the topical treatment of skin and soft tissue bacterial infections
S. Salatin (2019)
10.15171/apb.2019.042
Anti-aging and Sunscreens: Paradigm Shift in Cosmetics
Shreya Shanbhag (2019)
10.17352/OJEB.000004
Antioxidants and Ageing
B. Paital (2017)
10.4103/1735-5362.236831
Improvement of dermal delivery of tetracycline using vesicular nanostructures
Azam Hasanpouri (2018)
10.1016/j.jff.2020.103834
Protective effects of polymethoxyflavone-rich cold-pressed orange peel oil against ultraviolet B-induced photoaging on mouse skin
Guijie Li (2020)
10.1016/j.tifs.2020.05.004
Phytosterols as the core or stabilizing agent in different nanocarriers
Maryam Mohammadi (2020)
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