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Microspheres For The Oral Delivery Of Insulin: Preparation, Evaluation And Hypoglycaemic Effect In Streptozotocin-induced Diabetic Rats

Huan Zhang, W. Wang, H. Li, Y. Peng, Zhiqing Zhang
Published 2018 · Chemistry, Medicine

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Abstract Insulin-loaded microspheres were prepared by alternating deposition film layers that were composed of insulin and poly(vinyl sulfate) potassium on the surface of poly(lactic acid) (PLA) microspheres. The preparation of the insulin-loaded microspheres was optimized by an orthogonal test design, and the relationship between drug loading (DL) and film layers was studied. The particle size, DL and encapsulation efficiency of the obtained insulin-loaded microspheres with 10 films were 5.25 ± 0.15 µm, 111.33 ± 1.15 mg/g and 33.7 ± 0.19%, respectively. Following this, the physical characteristics of the insulin-loaded microspheres were investigated. The results from scanning electron microscopy and a laser particle size analyzer (LPSA) indicated the spherical morphology, rough surface and increasing particle sizes of the insulin-loaded microspheres, which were compared to those of PLA microspheres. An in vitro release study showed that the insulin-loaded microspheres were stable in HCl solution (pH 1.0) and released insulin slowly in phosphate-buffered solution (pH 6.8). Finally, the drug efficacy of the prepared insulin-loaded microspheres via oral administration was evaluated in rats with diabetes induced by streptozotocin, and an obvious dose-dependent hypoglycemic effect was observed. This preliminary data could illustrate the prospect of using microspheres for the oral delivery of insulin.
This paper references
Adsorption of insulin on metal surfaces in relation to association behavior
T. Arnebrant (1988)
Novel biodegradable and pH-sensitive poly(ester amide) microspheres for oral insulin delivery.
P. He (2012)
Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach.
A. des Rieux (2006)
Protective effect of zingerone on increased vascular contractility in diabetic rat aorta.
S. Ghareib (2016)
Microemulsions for oral delivery of insulin: design, development and evaluation in streptozotocin induced diabetic rats.
G. Sharma (2010)
Formulation optimization of water-in-oil-water multiple emulsion for intestinal insulin delivery.
Y. Onuki (2004)
The preparation of poly (lactic acid) microspheres
DG Li (2005)
Pancreatic extracts in the treatment of diabetes mellitus: preliminary report. 1922.
F. Banting (1991)
Biodegradable nanoparticles loaded with insulin-phospholipid complex for oral delivery: preparation, in vitro characterization and in vivo evaluation.
F. Cui (2006)
Human amylin proteotoxicity impairs protein biosynthesis, and alters major cellular signaling pathways in the heart, brain and liver of humanized diabetic rat model in vivo
Amro Ilaiwy (2016)
Alternative routes of insulin delivery
D. Owens (2003)
Novel Long-circulating Liposomes Containing Peptide Library-lipid Conjugates: Synthesis and In Vivo Behavior
E. Riché (2004)
Vesicles from Pluronic/poly(lactic acid) block copolymers as new carriers for oral insulin delivery.
X. Y. Xiong (2007)
Treatment with angiotensin-(1-9) alleviates the cardiomyopathy in streptozotocin-induced diabetic rats.
H. Zheng (2015)
In vitro and in vivo evaluation of insulin microspheres containing protease inhibitor.
M. Jelvehgari (2011)
Physicochemical characterization of spray-dried PLGA/PEG microspheres, and preliminary assessment of biological response
Curie Javiya (2016)
Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration.
Wei Wei (2008)
Layer-by-layer deposited nano- and micro-assemblies for insulin delivery: a review.
K. Yoshida (2014)
Antidiabetic Potentials of Various Ethno-Medicinal Plants of Rajasthan
E. Menghani (2010)
Microencapsulation: A promising technique for controlled drug delivery
M. Singh (2010)
Release profile of insulin from pH-sensitive hydrogel and its hypoglycemic effect by oral administration
Yongli Shi (2016)
Preparation and characterization of insulin-surfactant complexes for loading into lipid-based drug delivery systems.
P. Li (2013)
Oral insulin delivery.
Carino (1999)
Evaluation of biodegradability on polyaspartamide-polylactic acid based nanoparticles by chemical hydrolysis studies
E. Craparo (2015)
The robustness and flexibility of an emulsion solvent evaporation method to prepare pH-responsive microparticles.
Suchada Nilkumhang (2009)
Global estimates of the prevalence of diabetes for 2010 and 2030.
J. Shaw (2010)
Novel biodegradable and pHsensitive poly(ester amide) microspheres for oral insulin
P He (2012)
Pancreatic Extracts in the Treatment of Diabetes Mellitus: Preliminary Report.
F. G. Banting (1962)
Interfacial adsorption of insulin conformational changes and reversibility of adsorption.
S. Mollmann (2006)
Biodegradable polymeric nanoparticles based drug delivery systems.
A. Kumari (2010)
Current challenges in non-invasive insulin delivery systems: a comparative review.
E. Khafagy (2007)

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