Online citations, reference lists, and bibliographies.

Scanning Electron Microscopy And In Vitro Cultivation Of Endophytic Bacteria From Potato Tubers Afflicted With Zebra Chip Disease

Qiongguang Liu, J. Chen, J. Munyaneza, E. Civerolo, C. Wallis
Published 2013 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Abstract Zebra chip disease (ZCD) of potato (Solanum tuberosum L.) has caused significant economic losses to the potato industry in the USA. ‘Candidatus Liberibacter solanacearum’ Liefting et al. has been associated with ZCD. This non-culturable bacterium dwells within the phloem of plants where other endophytic bacteria may also live. Knowledge concerning phloem colonization by ‘Ca. L. solanacearum’ and other endophytic bacteria may provide a better understanding about disease biology and could facilitate the development of novel disease management strategies. In this study, endophytic bacteria in potato tubers afflicted with ZCD were evaluated by scanning electron microscopy (SEM), in vitro cultivation, and PCR analyses. Bacillus, coccus, and filamentous bacterial cells were observed by SEM in phloem tissue of ZCD-diseased tubers, but were absent in healthy potato tubers. Fifty-one bacterial isolates were obtained in vitro from ZCD-affected tubers and 34 isolates were from non-ZCD tubers. Comparison of 16S rDNA sequences identified bacteria belonging to 16 genera. Seven (Brachybacterium, Enterobacter, Microbacterium, Paenibacillus, Staphylococcus, Stenotrophomonas and Variovorax) were only isolated from ZCD-affected potato tubers, whereas four (Bosea, Nocardia, Sphingomonas and Sphingopyxis) were only isolated from non-ZCD potato tubers. However, a more sensitive PCR analysis diminished the specific association of the bacteria to either ZCD or non-ZCD tubers, suggesting that infection by ‘Ca. L. solanacearum’ influenced the titres of other endophytic bacteria in potato tubers.
This paper references
10.1094/PHYTO-95-0708
Two Xylella fastidiosa Genotypes Associated with Almond Leaf Scorch Disease on the Same Location in California.
J. Chen (2005)
10.1128/AEM.68.5.2261-2268.2002
Response of Endophytic Bacterial Communities in Potato Plants to Infection with Erwinia carotovora subsp. atroseptica
B. Reiter (2002)
10.1007/s002480000096
Analysis of endophytic bacterial communities of potato by plating and denaturing gradient gel electrophoresis (DGGE) of 16S rDNA based PCR fragments
P. Garbeva (2006)
10.1111/j.1469-8137.1991.tb00031.x
Colonization of wheat (Triticum vulgare L.) by N2‐fixing cyanobacteria: II. An ultrastructural study
M. Gantar (1991)
10.1094/Phyto-73-1510
Periwinkle wilt bacterium: axenic culture, pathogenicity and relationships to other Gram-negative, xylem-inhabiting bacteria.
M. Davis (1983)
10.1094/PDIS-93-6-0574
Association of 'Candidatus Liberibacter solanacearum' with Zebra Chip Disease of Potato Established by Graft and Psyllid Transmission, Electron Microscopy, and PCR.
G. Secor (2009)
10.1099/ijs.0.007377-0
'Candidatus Liberibacter solanacearum', associated with plants in the family Solanaceae.
L. Liefting (2009)
10.1016/J.FEMSEC.2004.08.006
Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi.
G. Berg (2005)
10.1139/W03-118
Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities.
A. Sessitsch (2004)
10.1111/J.1574-6968.2007.00918.X
Bacterial endophytes: recent developments and applications.
R. Ryan (2008)
10.1126/science.188.4192.1018
Plant Mycoplasmas: A Cultivable Spiroplasma Causes Corn Stunt Disease
D. L. Williamson (1975)
10.1139/m97-035
Bacterial endophytes in cotton: location and interaction with other plant-associated bacteria
A. Quadt-Hallmann (1997)
10.1080/07352680091139169
Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
A. V. Sturz (2000)
10.1128/aem.58.8.2691-2693.1992
Isolation of endophytic streptomyces strains from surface-sterilized roots.
P. Sardi (1992)
10.1111/j.1574-6941.2002.tb00903.x
Cultivation-independent population analysis of bacterial endophytes in three potato varieties based on eubacterial and Actinomycetes-specific PCR of 16S rRNA genes.
A. Sessitsch (2002)
10.1093/nar/8.19.4321
Rapid isolation of high molecular weight plant DNA.
M. G. Murray (1980)
10.1094/PDIS-93-3-0208
A New 'Candidatus Liberibacter' Species Associated with Diseases of Solanaceous Crops.
L. Liefting (2009)
10.4141/cjps74-019
ENDOPHYTIC BACTERIAL FLORA IN SOLANUM TUBEROSUM AND ITS SIGNIFICANCE IN BACTERIAL RING ROT DIAGNOSIS
S. H. Boer (1974)
10.1126/science.188.4192.1015
Corn Stunt Spiroplasma: Isolation, Cultivation, and Proof of Pathogenicity
T. Chen (1975)
10.1046/J.1365-3059.1999.00351.X
Endophytic bacterial communities in the periderm of potato tubers and their potential to improve resistance to soil‐borne plant pathogens
Sturz (1999)
A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy
Mj Karnovsky (1965)
10.1016/J.MIMET.2003.08.009
Review and re-analysis of domain-specific 16S primers.
G. C. Baker (2003)
10.1016/0304-4238(91)90139-P
Bacteria as a cause of vascular occlusion in cut fronds of Adiantum raddianum: a scanning electron microscope study
W. Doorn (1991)
10.1094/Phyto-73-217
Effect of Seed Piece Inoculation with Plant Growth-Promoting Rhizobacteria on Populations ofErwinia carotovoraon Potato Roots and in Daughter Tubers
J. Kloepper (1983)
Bacterial endophytes in agricultural crops. Can
J. HALLMANN (1997)
10.1139/M97-131
Bacterial endophytes in agricultural crops
J. Hallmann (1997)
10.1016/S0735-2689(01)80001-0
Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
A. V. Sturz (2000)
10.1094/PDIS-92-11-1547
Co-cultivation of 'Candidatus Liberibacter asiaticus' with Actinobacteria from Citrus with Huanglongbing.
M. Davis (2008)
Effect of seed piece inoculation with plant-growth promoting rhizobacteria on populations of Erwinia carotovora on potato roots and daughter
J. W. KLOEPPER (1983)
Endophytic bacterial communities
V. STURZ (1999)
Isolation of endophytic Streptomyces strains from surface-disinfested roots
R. SARDI (1992)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar