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Competitive Elisa For Quantifying Small Amounts Of Aflatoxin B1

M. Aldao, M. Carpinella, M. Corelli, G. Herrero
Published 1995 · Chemistry

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An indirect ELISA was developed to quantify aflatoxin B1 (AFB1). The detection limit was 0.025 ng ml‐1. The test used polyvinyl chloride (PVC) plates, activated with AFB1 bound to bovine serum albumin (BSA). Polyclonal antibodies were raised in rabbits against AFB1‐BSA. The specific anti‐AFB1antibodies were recovered from the crude antiserum by affinity chromatography from a column containing immobilized BSA on Nylon 6–6. Goat anti‐rabbit IgG antibodies bound to peroxidase were used to detect the rabbit IgG anti‐AFB1 antibodies bound to PVC plates. The colour developed by the subsequent enzyme conversion of the substrate was detected by spectrophotometry. The developed colour gave clear absorbance differences at varying doses of AFB1. Cross‐reactivity with aflatoxin B2, aflatoxin G1 and aflatoxin G2 was measured, showing percentages of 5.43, 64.5 and 5.07 respectively.
This paper references
10.1016/0041-008X(91)90168-E
Pharmacokinetics of intratracheally administered aflatoxin B1.
R. Coulombe (1991)
10.1016/0003-2697(82)90677-7
A dot-immunobinding assay for monoclonal and other antibodies.
R. Hawkes (1982)
10.1016/0300-483X(92)90040-L
Modulation of aflatoxin B1 biotransformation in rabbit pulmonary and hepatic microsomes.
J. M. Daniels (1992)
10.1016/0278-6915(86)90019-0
Mycotoxins: Formation, Analysis and Significance
J. Smith (1985)
10.1128/AEM.60.1.106-110.1994
Inhibition of aflatoxin production by surfactants.
S. B. Rodríguez (1994)
10.1016/0009-8981(94)90043-4
A rapid and sensitive sandwich enzyme immunoassay for low concentration of albumin in human urine.
G. Chiabrando (1994)
10.1016/S0040-4020(01)83429-3
Mycotoxin immunoassays (with special reference to elisas)
M. Morgan (1989)
10.1093/CARCIN/14.11.2423
Evaluation of the post-initiation effects of oltipraz on aflatoxin B1-induced preneoplastic foci in a rat model of hepatic tumorigenesis.
Y. Maxuitenko (1993)
10.1002/JSFA.2740370913
Aflatoxin determination in peanut butter by enzyme‐linked immunosorbent assay
M. Morgan (1986)
Influence of specificity and affinity of antibodies on the kinetics of a sandwich enzyme immunoassay for human IgG.
M. Aldao (1984)
10.1007/BF02637723
Dietary factors and aflatoxin toxicity: I. comparison of the effect of two diets supplemented with aflatoxin B1 upon two different strains of rats
R. Alfin-slater (1975)
Official Methods of Analysis
W. Horwitz (1980)
10.1093/JAOAC/74.5.827
Comparison of an ELISA-based screening test with liquid chromatography for the determination of aflatoxins in corn.
R. W. Beaver (1991)
10.1093/JAOAC/69.5.904
Enzyme-linked immunosorbent assay of aflatoxin B1 in naturally contaminated corn and cottonseed.
B. P. Ram (1986)
10.1016/0740-0020(87)90030-X
The detection of aflatoxin B1 in peanut kernels, peanut butter and maize using a monoclonal antibody based enzyme immunoassay.
A. Candlish (1987)
10.1016/S0168-8278(05)80587-2
Point mutations of the P53 gene, human hepatocellular carcinoma and aflatoxins.
A. Gerbes (1993)



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