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Indications For An Active Process Underlying Spontaneous And Radiation-induced Micronucleation In L929 Cells.

M. Abend, S. Frombeck, D. van Beuningen
Published 1999 · Biology, Medicine

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PURPOSE To investigate the mechanism of micronucleus formation in irradiated L929 cells. MATERIALS AND METHODS Radiation-induced micronuclei (MN) of L929 cells isolated at 48 and 72 h after irradiation were processed for detection of DNA-laddering and higher-order chromatin fragments using conventional gel electrophoresis and pulse-field gel electrophoresis. Quantification of double-strand breaks in micronuclei and nuclei was performed with the TdT assay and quantified using image analysis. The number of binucleated cells containing micronuclei (cytochalasin B method) was counted after application of three unspecific endonuclease inhibitors (aurin, ATA, spermine), a topoisomerase II inhibitor (VM-26), administration of two PKC inhibitors (H-7, Gö6983) and after addition of N-acetylsphingosine (C2-ceramide). PKC activity was determined by measuring the incorporation of [gamma-32P]ATP into a suitable specific substrate. Proliferation was measured by detection of PCNA, RFP-A and BrdU (30-min pulse labelling) using both conventional immunofluorescence and laser scanning microscopy. RESULTS (1) Higher chromatin fragments accumulated in MN with a size as they occur during early stages of apoptosis; (2) the frequency of MN was influenced by drugs known to play an important role in signalling and execution of apoptosis (endonucleases, topoisomerase II, protein kinases, ceramide); (3) MN are characterized by a reduced transcription ability (PCNA, RFP-A). CONCLUSIONS A proportion of L929 MN may be formed by an active process comparable with the early stages of apoptosis; it may play a role in the re-organization of the damaged genome.
This paper references
Cell death and DNA fragmentation induced by liposomal platinum(II) complex, L-NDDP in A2780 and A2780/PDD cells.
I. Han (1994)
bcl-2 protein inhibits etoposide-induced apoptosis through its effects on events subsequent to topoisomerase II-induced DNA strand breaks and their repair.
S. Kamesaki (1993)
Apoptosis and its modulation in human promyelocytic HL-60 cells treated with DNA topoisomerase I and II inhibitors.
R. Bertrand (1993)
The role of the sphingomyelin pathway and protein kinase C in radiation-induced cell kill.
Z. Fuks (1995)
Microfilament-disrupting agents prevent the formation of apoptotic bodies in tumor cells undergoing apoptosis.
T. Cotter (1992)
Activation of a Ca2+−Mg2+‐dependent endonuclease as an early event in castration‐induced prostatic cell death
N. Kyprianou (1988)
X chromosome inactivation and micronuclei in normal and Turner individuals
John C. Hando (1997)
DNA double-strand break distributions in X-ray and alpha-particle irradiated V79 cells: evidence for non-random breakage.
H. C. Newman (1997)
Kinetochore immunofluorescence in micronuclei: a rapid method for the in situ detection of aneuploidy and chromosome breakage in human fibroblasts.
U. Hennig (1988)
Inhibition of angiogenesis by aurintricarboxylic acid.
Gagliardi Ar (1994)
Mg(2+)-dependent cleavage of DNA into kilobase pair fragments is responsible for the initial degradation of DNA in apoptosis.
X. Sun (1994)
Isolation of micronuclei: high yield by sucrose gradient versus maximum purity by cell sorting.
M. Abend (1996)
Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: correlation with suppression of endonuclease activity
A. Batistatou (1991)
Protein kinase C involvement in apoptosis.
M. Lucas (1995)
Isolation of high-molecular-weight DNA from mammalian cells.
M. Gross-Bellard (1973)
Exhaustive physical exercise increases frequency of micronuclei.
C. Schiffl (1997)
Inhibition of topoisomerase II by aurintricarboxylic acid: implications for mechanisms of apoptosis.
D. Catchpoole (1994)
Endonuclease activities associated with high molecular weight and internucleosomal DNA fragmentation in apoptosis.
P. Walker (1994)
Classification of micronuclei in murine erythrocytes: immunofluorescent staining using CREST antibodies compared to in situ hybridization with biotinylated gamma satellite DNA.
B. Miller (1991)
Coming to terms with death: apoptosis in cancer and immune development.
J. Ashwell (1994)
The topoisomerase II inhibitor teniposide (VM-26) induces apoptosis in unstimulated mature murine lymphocytes.
C. Roy (1992)
Degradation of chromatin in apoptotic cells.
P. Walker (1995)
Use of the micronucleus assay for the selective detection of radiosensitivity in BUdR-unincorporated cells after pulse-labelling of exponentially growing tumour cells.
S. Masunaga (1990)
Internucleosomal DNA cleavage and neuronal cell survival/death
A. Batistatou (1993)
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
Micronuclei in cytokinesis-blocked lymphocytes as an index of occupational exposure to alkylating cytostatic drugs.
J. Yager (1988)
Inhibition of both etoposide-induced DNA fragmentation and activation of poly(ADP-ribose) synthesis by zinc ion.
T. Shimizu (1990)
Programmed cell death induced by ceramide.
L. Obeid (1993)
Protein phosphorylation in apoptosis.
B. Gjertsen (1995)
Fractionated ionizing radiation accelerates loss of amplified MDR1 genes harbored by extrachromosomal DNA in tumor cells.
A. Sanchez (1998)
Does bulk damage to DNA explain the cytostatic and cytotoxic effects of topoisomerase II inhibitors?
D. Gewirtz (1991)
Gamma-irradiation-induced micronuclei from mouse hepatoma cells accumulate high levels of the tumor suppressor protein p53.
C. Unger (1994)
Inhibition of ceramide-induced apoptosis by Bcl-2.
S. J. Martin (1995)
Role of nucleases in apoptosis.
B. Zhivotovsky (1994)
The role of DNA fragmentation in apoptosis.
C. Bortner (1995)
Detection of whole chromosomes in micronuclei of cytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridization.
H. Norppa (1993)
DNA fragmentation induced by cytotoxic T lymphocytes can result in target cell death.
C. Helgason (1993)
Analysis of micronuclei induced by 2-chlorobenzylidene malonitrile (CS) using fluorescence in situ hybridization with telomeric and centromeric DNA probes, and flow cytometry.
B. Miller (1993)
Evaluation of a modified micronucleus assay.
M. Abend (1996)
Micronucleus frequencies in lymphoblastoid cell lines measured with the cytokinesis-block technique and flow cytometry.
A. Slavotinek (1995)
Human DNA topoisomerase II: biochemistry and role in chemotherapy resistance (review).
S. Withoff (1996)
Cellular signalling in programmed cell death (apoptosis).
D. McConkey (1990)
Micronuclei, centromere-positive micronuclei and chromosome nondisjunction in cytokinesis blocked human lymphocytes following mitomycin C or vincristine treatment.
A. Sgura (1997)
Ca2+/Mg(2+)-dependent endonuclease from human spleen: purification, properties, and role in apoptosis.
J. M. Ribeiro (1993)
Attenuation of ceramide-induced apoptosis by diglyceride in human myeloid leukemia cells.
W. D. Jarvis (1994)
Requirement of AP-1 for Ceramide-induced Apoptosis in Human Leukemia HL-60 Cells (*)
H. Sawai (1995)
XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure
C. Clemson (1996)
p53 accumulates in micronuclei after treatment with a DNA breaking chemical, methylnitrosourea, and with the spindle poison, vinblastine.
C. Granetto (1996)
Tumor radiosensitivity prediction by the cytokinesis-block micronucleus assay.
Y. Shibamoto (1991)
Apoptosis, oncosis, and necrosis. An overview of cell death.
G. Majno (1995)
Correlation of micronucleus and apoptosis assays with reproductive cell death.
M. Abend (1995)
Chromatin loop size in human leukemia (HL-60) cells.
Y. Tohno (1995)
Calcium-activated DNA fragmentation in rat liver nuclei.
D. P. Jones (1989)

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M. Abend (2003)
Mechanisms of Apoptosis Modulation and their Contribution to Genomic Instability in Tumor Cells
V. Vukicevic (2004)
Influence of altered apoptosis in human lymphoblastoid cell lines on micronucleus frequency.
V. Vukićević (2004)
Fate of micronuclei and micronucleated cells.
H. Hintzsche (2017)
Radiation‐induced gene expression in MCF‐7 cells
T. Staßen (2003)
Genetic activities in micronuclei: is the DNA entrapped in micronuclei lost for the cell?
M. Terradas (2010)
Impact of Mitochondrial DNA on Radiation Sensitivity of Transformed Human Fibroblast Cells: Clonogenic Survival, Micronucleus Formation and Cellular ATP Level
Y. Yoshioka (2004)
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Pei-Tzu Li (2007)
Impact of mitochondrial DNA on hypoxic radiation sensitivity in human fibroblast cells and osteosarcoma cell lines.
H. Yamazaki (2008)
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