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A LARGE SCALE METHOD FOR PREPARATION OF PLANT THYLAKOIDS FOR USE IN BODY WEIGHT REGULATION

S. C. Emek, A. Szilágyi, Hans-Erik Åkerlund, P. Albertsson, Rickard Köhnke, A. Holm, C. Erlanson-Albertsson
Published 2010 · Chemistry, Medicine

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A method for preparation of thylakoids from plant leaves on a large scale is described. The method involves: 1) disruption of the cells with a blender followed by filtration to remove large cell debris and non disrupted cells. 2) precipitation of the thylakoids by adjusting the pH to the isoelectric point, pH 4.7. 3) a washing step by dilution of the precipitate in water followed by precipitation at the same pH. 4) concentration of the precipitate by freeze- thawing or freeze -drying to get the final product. The product is characterized, with respect to protein composition, by SDS-PAGE and mass-spectroscopy, the content of carotenoids, particularly the xanthophylls violaxanthin, antheraxanthin, and zeaxanthin. The thylakoid preparation has about the same capacity to inhibit pancreatic lipase/colipase activity as thylakoids prepared by standard laboratory methods using sucrose in the medium and centrifugation. In a study with mice, it was found that, when the thylakoids were added to the food over 32 days, they significantly reduced the body weight gain and the percentage body fat. The large scale method described here allows studies on the effect of thylakoids in appetite regulation on experimental animals in a longer lasting time and also on humans.
This paper references
10.1016/S1360-1385(01)02021-0
A quantitative model of the domain structure of the photosynthetic membrane.
P. Albertsson (2001)
10.1016/S0005-2728(89)80347-0
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
R. J. Porra (1989)
10.1016/0005-2728(88)90010-2
Separation and characterization of stroma and grana membranes — evidence for heterogeneity in antenna size of both Photosystem I and Photosystem II
E. Andréasson (1988)
10.1001/JAMA.272.18.1413
Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration. Eye Disease Case-Control Study Group.
J. Seddon (1994)
Separation of spinach leaf mitochondria according to surface properties: Partition in aqueous polymer two-phase systems
P. Gardeström (1987)
Erlanson-Albertsson, C. Thylakoids suppress appetite by increasing cholecystokinin resulting in lower food intake and body weight in high-fat food mice
R Köhnke
Preparation of inside-out vesicles from membrane-paired randomized chloroplast lamella
B. Andersson (1979)
Molecular mechanisms of photosynthesis; Blackwell Science: Oxford=London= Edinburgh=Malden=Victoria=Paris
R. E. Blankenship (2002)
Erlanson-Albertsson, C. Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes
P Å Albertsson (2007)
10.1021/PR050469Z
Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells.
Henrik Everberg (2006)
10.1152/AJPENDO.2000.279.2.E293
Effect of high-fat diet, surrounding temperature, and enterostatin on uncoupling protein gene expression.
C. Rippe (2000)
10.1038/nrm1525
The complex architecture of oxygenic photosynthesis
N. Nelson (2004)
10.1080/00365520902803499
Thylakoids promote release of the satiety hormone cholecystokinin while reducing insulin in healthy humans
Rickard Köhnke (2009)
10.1073/PNAS.0503472102
AtFtsH6 is involved in the degradation of the light-harvesting complex II during high-light acclimation and senescence.
Agnieszka Zelisko (2005)
Preparation of highly enriched photosystem II membrane by a non-detergent method
P. Svensson (1989)
Separation and characterization of stroma and grana membranes-evidence for heterogeneity in antenna size of both Photosystem I and Photosystem II. Biochim
E. Andreasson (1988)
10.1002/ptr.2855
Thylakoids suppress appetite by increasing cholecystokinin resulting in lower food intake and body weight in high‐fat fed mice
Rickard Köhnke (2009)
10.3109/00365528809093839
Alternatives to the acid-oriented approach to ulcer disease: does 'cytoprotection' exist in man? A new classification of antiulcer agents.
S. Szabo (1988)
Lipoxygenases: Occurence, function and catalyses
A. Liavenchanka (2006)
10.1007/BF00034068
Preparation of highly enriched photosystem II membrane vesicles by a non-detergent method
P. Svensson (2004)
10.1016/0005-2736(80)90186-8
A mechanism for the formation of inside-out membrane vesicles. Preparation of inside-out vesicles from membrane-paired randomized chloroplast lamellae.
B. Andersson (1980)
10.1016/0005-2736(79)90280-3
Isoelectric points of spinach thylakoid membrane surfaces as determined by cross partition.
H. Akerlund (1979)
10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2
Probability‐based protein identification by searching sequence databases using mass spectrometry data
D. N. Perkins (1999)
Erlanson-Albertsson, C. Thylakoids promote release of satiety hormone cholecystokinin while reducing insulin in healthy humans
R Köhnke
10.1159/000198689
Clinico-pathological conditions associated with pancreas divisum.
J. Sahel (1982)
10.1097/00006676-198709000-00007
Secretion of Pancreatic Lipase and Colipase from Rat Pancreas
C. Erlanson-Albertsson (1987)
10.1016/S1360-1385(02)02326-9
Cutting edge of chloroplast proteolysis.
Z. Adam (2002)
10.1111/J.1399-3054.1997.TB00007.X
The xanthophyll cycle, its regulation and components
Marie Eskling (1997)
10.1104/PP.79.1.72
Surface properties of right side-out plasma membrane vesicles isolated from barley roots and leaves.
L. Koerner (1985)
10.1007/BF00032588
Regulation of violaxanthin de-epoxidase activity by pH and ascorbate concentration
C. Bratt (2004)
10.1002/9780470758472
Molecular mechanisms of photosynthesis
R. Blankenship (2002)
10.1016/0079-6107(79)90016-6
Plant lipids and their role in membrane function.
P. Quinn (1978)
10.1016/J.JPLPH.2005.11.006
Lipoxygenases: occurrence, functions and catalysis.
A. Liavonchanka (2006)
10.1042/BJ20061463
Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes on pancreatic lipase/co-lipase.
P. Albertsson (2007)
10.1016/0005-2736(83)90189-X
Isoelectric points of membrane surfaces of three spinach chloroplast classes determined by cross-partition
H. Westrin (1983)
10.1007/BF00018372
Loose association of ribulose 1,5-bisphosphate carboxylase/oxygenase with chloroplast thylakoid membranes
H. Mori (2004)
Erlanson-Albertsson, C. Effect of high-fat diet, surrounding temperature, and enterostatin on uncoupling protein gene expression
C Rippe (2000)
10.1007/BF00034864
Leaf Xanthophyll content and composition in sun and shade determined by HPLC
S. S. Thayer (2004)
10.1016/0076-6879(87)48042-7
[40] Separation of spinach leaf mitochondria according to surface properties: Partition in aqueous polymer two-phase systems
P. Gardeström (1987)
Dietary carotenoids, vitamin-A, vitamin-C, and vitamin-E, and advanced age-related macular degeneration
J. M. Seddon (1994)
10.1146/ANNUREV.ARPLANT.53.100301.135248
The lipoxygenase pathway.
I. Feussner (2002)
10.1023/A:1005868026374
Violaxanthin accessibility and temperature dependency for de-epoxidation in spinach thylakoid membranes
Per-Ola Arvidsson (2004)



This paper is referenced by
10.1017/S0007114511001267
Chloroplast thylakoids reduce glucose uptake and decrease intestinal macromolecular permeability.
C. Montelius (2011)
Intestinal regulation of hunger and reward. Studies with thylakoids.
Eva-Lena Stenblom (2016)
10.1186/s12986-016-0160-4
Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice
K. Stenkula (2017)
10.1016/J.JFOODENG.2009.12.025
Post harvest improvement of zeaxanthin content of vegetables
Maria Clausén (2010)
10.1002/jsfa.6034
Pancreatic lipase-colipase binds strongly to the thylakoid membrane surface.
S. C. Emek (2013)
Thylakoid membranes from green plants affect appetite and promote body weight loss
C. Montelius (2015)
10.1016/J.FOODHYD.2010.12.004
Pigments protect the light harvesting proteins of chloroplast thylakoid membranes against digestion by gastrointestinal proteases
S. C. Emek (2011)
10.4172/2165-7904.1000238
Decreased Urge for Palatable Food after a Two-month Dietary Intervention with Green-plant Membranes in Overweight Women
Eva-Lena Stenblom (2014)
10.1039/c4fo01074d
Heat-induced aggregation of thylakoid membranes affect their interfacial properties.
K. Östbring (2015)
10.1016/j.appet.2013.04.022
Supplementation by thylakoids to a high carbohydrate meal decreases feelings of hunger, elevates CCK levels and prevents postprandial hypoglycaemia in overweight women
Eva-Lena Stenblom (2013)
10.1016/j.clnu.2013.12.009
Dietary thylakoids suppress blood glucose and modulate appetite-regulating hormones in pigs exposed to oral glucose tolerance test.
C. Montelius (2014)
10.1016/j.foodchem.2016.08.093
Understanding leaf membrane protein extraction to develop a food-grade process.
Angelica Tamayo Tenorio (2017)
10.1080/09168451.2017.1409070
Intake of green-plant membrane with dietary oil suppresses postprandial hypertriglyceridemia in rats via promoting excretion of bile acids
Hiroko Matsuda (2018)
10.1007/978-3-030-16230-6_8
Cell Separation and Disruption, Product Recovery, and Purification
S. Y. Lee (2019)
10.1080/07315724.2014.1003999
Acute Effects of a Spinach Extract Rich in Thylakoids on Satiety: A Randomized Controlled Crossover Trial
C. Rebello (2015)
10.1016/j.appet.2015.04.051
Consumption of thylakoid-rich spinach extract reduces hunger, increases satiety and reduces cravings for palatable food in overweight women
Eva-Lena Stenblom (2015)
10.1017/jns.2012.29
Feeding spinach thylakoids to rats modulates the gut microbiota, decreases food intake and affects the insulin response
C. Montelius (2013)
Dietary Strategies to Influence Appetite: Effects of Oat Beta-Glucan and Thylakoids from Spinach on Satiety and Reward-induced Eating Behavior
C. Rebello (2015)
10.1016/j.abb.2015.02.022
Carotenoids and their conversion products in the control of adipocyte function, adiposity and obesity.
M. L. Bonet (2015)
10.1016/J.TIFS.2019.03.028
Plants of the genus Spinacia: From bioactive molecules to food and phytopharmacological applications
B. Salehi (2019)
10.1152/ajpregu.00212.2016
Dietary thylakoids reduce visceral fat mass and increase expression of genes involved in intestinal fatty acid oxidation in high-fat fed rats.
Eva-Lena Stenblom (2016)
10.1016/J.PROFOO.2011.09.212
A novel emulsifier from spinach with appetite regulation abilities
M. Rayner (2011)
10.1186/s12986-016-0128-4
Dietary green-plant thylakoids decrease gastric emptying and gut transit, promote changes in the gut microbial flora, but does not cause steatorrhea
Eva-Lena Stenblom (2016)
10.1002/jsfa.8627
Characteristics and functionality of appetite-reducing thylakoid powders produced by three different drying processes.
K. Östbring (2018)
10.3390/foods9050669
Effects of Storage Conditions on Degradation of Chlorophyll and Emulsifying Capacity of Thylakoid Powders Produced by Different Drying Methods †
K. Östbring (2020)
10.1021/bk-2012-1093.ch029
Thylakoids Promote Satiety in Healthy Humans. Metabolic Effects and Mechanisms
C. Erlanson-Albertsson (2012)
10.1016/j.appet.2014.06.101
Body weight loss, reduced urge for palatable food and increased release of GLP-1 through daily supplementation with green-plant membranes for three months in overweight women
C. Montelius (2014)
10.1038/ijo.2015.142
Gut fat signaling and appetite control with special emphasis on the effect of thylakoids from spinach on eating behavior
C. Rebello (2015)
10.1039/c3fo60651a
The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/co-lipase activity.
K. Östbring (2014)
10.1007/978-3-319-39126-7_15
Carotenoids in Adipose Tissue Biology and Obesity.
M. L. Bonet (2016)
10.1024/0300-9831/a000556
Thylakoids: A Novel Food-Derived Supplement for Obesity - A Mini-Review.
Sahar Foshati (2019)
10.1186/s12937-020-00601-4
The effects of spinach-derived thylakoid supplementation in combination with calorie restriction on anthropometric parameters and metabolic profiles in obese women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled clinical trial
F. P. F. Tabrizi (2020)
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