Online citations, reference lists, and bibliographies.
← Back to Search

Doxycycline-coated Sutures Improve The Suture-holding Capacity Of The Rat Achilles Tendon

B. Pasternak, Anna Missios, A. Askendal, P. Tengvall, P. Aspenberg
Published 2007 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Background There is evidence of high matrix metalloproteinase (MMP) activity around sutures inserted into tendons. This probably results in tissue breakdown, allowing the suture to cut through the tendon, and thus contributes to repair-site elongation and gap formation. We therefore hypothesized that treatment with the MMP inhibitor doxycycline would improve the sutureholding capacity of tendon. Animals, methods and results In the first sub-study, rats received a suture in the Achilles tendon. One group was treated with systemic doxycycline and the other received no treatment. At 3, 5, and 7 days, suture-holding capacity was measured mechanically. The pull-out force and energy were reduced in all tendons, at 3 days compared to freshly inserted sutures, but no further reduction was detected at later time points. Doxycycline- treated tendons showed improved suture-holding capacity as measured by higher energy uptake than in untreated tendons. Force at failure showed a trend towards improvement. The effect was most evident on day 3. In the second sub-study, sutures were coated with doxycycline. At 3 days, local doxycycline treatment caused improved suture-holding capacity—as measured by higher force at failure and higher energy uptake. Interpretation We provide proof of a novel treatment principle. MMP inhibitor-coated sutures improve suture-holding capacity during early repair of collagenous tissues.
This paper references
A FORTAN Program for the Analysis of Ellipsometer Measurements
F L Mccrackin (1969)
Achilles tendon rupture: surgical versus nonsurgical treatment
R Lynch (2004)
10.1002/BIP.1978.360170711
Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air–water interface
J. De Feijter (1978)
10.1053/JHSU.2000.JHSU25A0260
An in vivo study of locking and grasping techniques using a passive mobilization protocol in experimental animals.
H. Hatanaka (2000)
10.1054/jhsb.2000.0547
Comparison of Postoperative Early Active Mobilization and Immobilization in Vivo Utilising a Four-Strand Flexor Tendon Repair
A. Wada (2001)
10.2106/00004623-199907000-00010
The effect of gap formation at the repair site on the strength and excursion of intrasynovial flexor tendons. An experimental study on the early stages of tendon-healing in dogs.
R. Gelberman (1999)
10.1016/J.BIOMATERIALS.2003.08.049
Surface immobilized bisphosphonate improves stainless-steel screw fixation in rats.
P. Tengvall (2004)
Ellipsometry as a tool to study the adsorption of synthetic and biopolymers at the air-water interface
J A De Feijter (1978)
10.1016/S0363-5023(83)80012-4
Elongation of the repair configuration following flexor tendon repair.
H. Seradge (1983)
10.1177/107110070602700209
Suture Holding Capacity of the Achilles Tendon during the Healing Period: An In Vivo Experimental Study in Rabbits
Yakup Yıldırım (2006)
10.1302/0301-620X.81B6.9700
Periacetabular osteotomy in the treatment of neurogenic acetabular dysplasia
S. Macdonald (1999)
10.1054/JHSB.1998.0212
The Aetiology of Acute Rupture of Flexor Tendon Repairs in Zones 1 and 2 of the Fingers During Early Mobilization
S. B. Harris (1999)
10.1002/JOR.1100120415
Effects of immobilization on achilles tendon healing in a rat model
G. Murrell (1994)
10.1016/S0736-0266(03)00185-2
Ex vivo static tensile loading inhibits MMP‐1 expression in rat tail tendon cells through a cytoskeletally based mechanotransduction mechanism
S. Arnoczky (2004)
10.1053/JHSU.2002.34006
Characterization of the tensile properties and histologic/biochemical changes in normal chicken tendon at the site of suture insertion.
C. McDowell (2002)
10.1046/J.1365-2168.2001.01649.X
Inhibition of matrix metalloproteinases enhances breaking strength of colonic anastomoses in an experimental model
I. Syk (2001)
Measurement of antibiotics in human body fluids: Techniques and significance
M Klassen (1996)
10.1051/JPHYSCOL:19831017
THE USE OF THE ISOSCOPE ELLIPSOMETER IN THE STUDY OF ADSORBED PROTEINS AND BIOSPECIFIC BINDING REACTIONS
M. Stenberg (1983)
Stress-deprivation of tendons results in alterations in the integrin profile and pericellular matrix of tendon cells
M Egerbacher (2006)
10.1016/J.AAEN.2003.11.004
Achilles tendon rupture: surgical versus non-surgical treatment.
R. Lynch (2004)
Doxycycline impairs tendon repair in rats.
B. Pasternak (2006)
10.3109/02844318109103414
Elongation in profundus tendon repair. A clinical and radiological study.
A. Ejeskär (1981)
10.2106/00004623-200201000-00012
Effect of Synergistic Wrist Motion on Adhesion Formation After Repair of Partial Flexor Digitorum Profundus Tendon Lacerations in a Canine Model in Vivo
C. Zhao (2002)
10.2174/1389557043403657
Matrix metalloproteinases and colon anastomosis repair: a new indication for pharmacological inhibition?
M. Ågren (2004)
10.1016/S0927-7765(99)00136-8
Quantification of adsorbed human serum albumin at solid interfaces: a comparison between radioimmunoassay (RIA) and simple null ellipsometry
J. Benesch (2000)
10.1016/S0927-7765(02)00151-0
Preparation of multilayer plasma protein films on silicon by EDC/NHS coupling chemistry
P. Tengvall (2003)
10.1067/MSY.2003.27
Doxycycline improves wound strength after intestinal anastomosis in the rat.
M. Siemonsma (2003)



This paper is referenced by
10.1097/JSA.0b013e318227b07b
Role of Metalloproteinases in Rotator Cuff Tear
R. Garofalo (2011)
10.3109/17453670903448257
Metalloproteinases and their inhibitors—diagnostic and therapeutic opportunities in orthopedics
B. Pasternak (2009)
10.1088/1748-6041/6/1/015014
Biomechanical properties of Achilles tendon repair augmented with a bioadhesive-coated scaffold.
Michael Brodie (2011)
10.1136/jisakos-2020-000451
Doxycycline improves tendon and cartilage pathologies in preclinical studies: current concepts
Robert S. Dean (2020)
10.1016/j.jse.2009.07.010
The effect of matrix metalloproteinase inhibition on tendon-to-bone healing in a rotator cuff repair model.
A. Bedi (2010)
10.1007/s11010-016-2710-5
Immunobiological factors aggravating the fatty infiltration on tendons and muscles in rotator cuff lesions
Finosh G. Thankam (2016)
10.1016/j.jsams.2008.03.005
Tendinopathy and drugs--potential implications for beneficial and detrimental effects on painful tendons.
K. Knobloch (2009)
10.1177/0363546509347366
Doxycycline-Mediated Inhibition of Matrix Metalloproteinases Improves Healing after Rotator Cuff Repair
A. Bedi (2010)
Towards an Understanding of the Mechanisms of Acellular Zone Formation in Sutured Tendons
S. A. Youha (2011)
10.2174/0929867324666161118141724
Bioactive Sutures: A Review of Advances in Surgical Suture Functionalisation.
F. Alshomer (2017)
10.1002/jor.22564
Enhancement of Achilles tendon repair mediated by matrix metalloproteinase inhibition via systemic administration of doxycycline
Michael W. Kessler (2014)
10.1159/000358811
Colonic Anastomosis with a Doxycycline-Coated Stent: An Experimental Study in a Porcine Model
YiFan Wang (2014)
10.1016/j.arthro.2017.10.045
Biomechanical, Histologic, and Molecular Evaluation of Tendon Healing in a New Murine Model of Rotator Cuff Repair.
A. Lebaschi (2018)
10.1111/wrr.12153
Mesenchymal stem cell‐coated sutures enhance collagen depositions in sutured tissues
J. Casado (2014)
10.2147/IJN.S217697
Doxycycline-Embedded Nanofibrous Membranes Help Promote Healing of Tendon Rupture
Chun-Jui Weng (2020)
10.11138/mltj/2013.3.1.051
Metalloproteases and tendinopathy.
A. Del Buono (2013)
10.1016/j.jse.2017.09.020
Synovial fluid biomarkers: association with chronic rotator cuff tear severity and pain.
Chien-An Shih (2018)
10.1002/jps.24610
Advanced Therapeutic Dressings for Effective Wound Healing--A Review.
J. Boateng (2015)
Advanced Therapeutic Dressings For Effective Wound Healing
J. Boateng (2015)
10.1177/0363546517716637
Therapeutic Effects of Doxycycline on the Quality of Repaired and Unrepaired Achilles Tendons
Q. Nguyen (2017)
10.1002/jbm.b.33753
Genipin crosslinker releasing sutures for improving the mechanical/repair strength of damaged connective tissue.
S. Sundararaj (2017)
10.1016/j.csm.2012.07.003
Biologics in the management of rotator cuff surgery.
Michael E. Angeline (2012)
10.1371/journal.pone.0168077
MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis
Finosh G. Thankam (2016)
10.1016/j.jse.2011.10.020
Metalloproteases and rotator cuff disease.
A. Del Buono (2012)
Thérapie par les cellules souches mésenchymateuses dans la guérison tendineuse chez le cheval
Céline Bourzac (2010)
Alterations in tendon microenvironment in response to mechanical load: potential molecular targets for treatment strategies.
Mohamed B Fouda (2017)
10.11138/JTS/2014.2.1.026
Degenerative disease in rotator cuff tears: what are the biochemical and histological changes?
S. De Giorgi (2014)
Semantic Scholar Logo Some data provided by SemanticScholar