Online citations, reference lists, and bibliographies.
← Back to Search

Disruption Of Epithelial Cell-matrix Interactions Induces Apoptosis

S. Frisch, H. Francis
Published 1994 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Cell-matrix interactions have major effects upon phenotypic features such as gene regulation, cytoskeletal structure, differentiation, and aspects of cell growth control. Programmed cell death (apoptosis) is crucial for maintaining appropriate cell number and tissue organization. It was therefore of interest to determine whether cell- matrix interactions affect apoptosis. The present report demonstrates that apoptosis was induced by disruption of the interactions between normal epithelial cells and extracellular matrix. We have termed this phenomenon "anoikis." Overexpression of bcl-2 protected cells against anoikis. Cellular sensitivity to anoikis was apparently regulated: (a) anoikis did not occur in normal fibroblasts; (b) it was abrogated in epithelial cells by transformation with v-Ha-ras, v-src, or treatment with phorbol ester; (c) sensitivity to anoikis was conferred upon HT1080 cells or v-Ha-ras-transformed MDCK cells by reverse- transformation with adenovirus E1a; (d) anoikis in MDCK cells was alleviated by the motility factor, scatter factor. The results suggest that the circumvention of anoikis accompanies the acquisition of anchorage independence or cell motility.
This paper references
Epitheliai-mesenchymal transitions
E. Hay (1990)
Monoclonai antibodies specific for Ela proteins: heterogeneity in Ela products
E. Harlow (1985)
Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton.
D. Ingber (1993)
Rule)'. 1993. Stabilization of the p53 tumor suppressor is induced by Ela and accompanies apoptosis
S Lowe
The induction of apoptosis occurs in all phases of the cell cycle
T. Cotter (1992)
Elevated levels of fibronectin receptor suppress the transformed phenotype of CHO cells
F. Giancotti (1990)
Four-day duration of tumor promoter exposure required to transform JB6 promotion-sensitive cells to anchorage independence.
L. Dion (1988)
10.1038/284555A0
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
Regulation of Bcl-2 oncoprotein levels with differentiation of human neuroblastoma cells.
M. Hanada (1993)
Carcinogenesis-a Comprehensive Survey
N. Colburn (1980)
10.1128/MCB.6.10.3470
Identification of separate domains in the adenovirus E1A gene for immortalization activity and the activation of virus early genes.
E. Morán (1986)
10.1083/JCB.107.4.1575
The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex
B. Gumbiner (1988)
Integrins. J. Clin. Invest
E Ruoslahti (1991)
10.1128/MCB.10.8.4370
Complementation by BCL2 and C-HA-RAS oncogenes in malignant transformation of rat embryo fibroblasts.
John Calvin Reed (1990)
10.1073/PNAS.90.3.786
Toward an understanding of the molecular mechanisms of physiological cell death.
D. Vaux (1993)
Wild-type p53 mediates apoptosis by Ela, which is inhibited by Elb
M. Debbas (1993)
Dynamics of membrane-cytuskeletun (fodrin ) organization during development of polarity in MDCK cells
W Nelson (1986)
10.1007/978-3-0348-7494-6_8
The role of E-cadherin and scatter factor in tumor invasion and cell motility.
J. Behrens (1991)
Differential expression of bcl-2 proto-oncogene in neuroblastuma and other human tumor liines of neural origin
J Reed (1991)
10.1101/GAD.7.4.546
Wild-type p53 mediates apoptosis by E1A, which is inhibited by E1B.
M. Debbas (1993)
10.1016/0092-8674(80)90473-0
Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts
A. Ben-Ze'ev (1980)
10.1083/JCB.106.3.829
Inhibitors of protein synthesis and RNA synthesis prevent neuronal death caused by nerve growth factor deprivation
D. P. Martin (1988)
10.1083/JCB.103.5.1751
Dynamics of membrane-skeleton (fodrin) organization during development of polarity in Madin-Darby canine kidney epithelial cells
W. J. Nelson (1986)
Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin.
John Calvin Reed (1991)
10.1126/SCIENCE.8430328
Death gives birth to the nervous system. But how?
M. Barinaga (1993)
Differential expression of bcl-2 proto-oncogene in neuroblas
J. Reed (1991)
Type I collagen Gel induces MDCK cells to become fusiform in shape and lose apical-basal polarity
A. Zuk (1989)
10.1016/0955-0674(93)90118-A
Apoptosis and signal transduction: clues to a molecular mechanism.
S. Lee (1993)
The extracellular matrix as a survival factor
J. Meredith (1993)
Role of the Elb 19,000 kD tumor
E. White (1988)
10.1128/MCB.12.6.2570
The 19-kilodalton adenovirus E1B transforming protein inhibits programmed cell death and prevents cytolysis by tumor necrosis factor alpha.
E. White (1992)
The adenovirus Ela proteins induce apoptusis
White (1992)
Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note.
S. Kaufmann (1989)
10.1091/MBC.4.9.953
The extracellular matrix as a cell survival factor.
J. Meredith (1993)
10.1016/0092-8674(86)90259-X
Arg-Gly-Asp: A versatile cell recognition signal
E. Ruoslahti (1986)
Dynamics of membrane - cytuskeletun ( fo - drin ) organization during development of polarity in MDCK cells
G. Ojakian (1986)
Identification of separate domains in the Ela gene for immortalization and the activation of virus early genes
E. Moran (1986)
Programmed cell death in terminally differentiating keratinocytes
C. McCall (1991)
Role of the Elb 19 , 000 kD tumor antigen in regulating early gene expression
R. Cipriani (1988)
10.1016/S1043-4682(10)80013-3
Cell adhesion in invasion and metastasis.
J. Behrens (1992)
10.1128/MCB.7.4.1326
Nonmitogenic morphoregulatory action of pp60v-src on multicellular epithelial structures.
S. Warren (1987)
Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etuposide, camptuthecin and other cytotoxic anticancer drugs
S. Kaufman (1989)
10.1016/b978-0-12-648360-4.x5001-9
Extracellular matrix influences on gene expression.
H. Slavkin (1982)
10.1128/JVI.20.3.570-582.1976
Type C particle-positive and type C particle-negative rat cell lines: characterization of the coding capacity of endogenous sarcoma virus-specific RNA.
E. Scolnick (1976)
10.1073/PNAS.88.20.9077
Antioncogenic effect of adenovirus E1A in human tumor cells.
S. Frisch (1991)
Apoptosis: The Molecular Basis of Cell Death
L. D. Tomei (1991)
Non-mitogenic morphoregulatury action
S. Warren (1987)
10.1083/JCB.17.2.375
JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA
M. Farquhar (1963)
10.1038/273345A0
Role of cell shape in growth control
J. Folkman (1978)
10.1016/0092-8674(81)90274-9
Tumor promoter-induced changes in the permeability of epithelial cell tight junctions
G. Ojakian (1981)
Differential expression of bcl - 2 proto - oncogene in neuroblas - tuma and other human tumor liines of neural origin
S. Halden (1991)
10.1016/S0076-6879(79)58170-1
An established but differentiated kidney epithelial cell line (MDCK).
M. Taub (1979)
10.1073/PNAS.89.16.7742
The adenovirus E1A proteins induce apoptosis, which is inhibited by the E1B 19-kDa and Bcl-2 proteins.
L. Rao (1992)
10.1126/SCIENCE.8430327
Cell death studies yield cancer clues.
J. Marx (1993)
The hepatocyte growth factor receptor is the c-met oncogene product
D. Bottaro (1991)
10.1016/0270-9139(91)90068-7
Hepatocyte growth factor receptor and the c-met oncogene Bottaro DP, Rubin JS, Faletto DL, Chan AM-L, Kmiecik TE, Vande Woude GF, Aaronson SA. Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science 1991;251:802?804
N. Fausto (1991)
10.1006/SCEL.1993.1020
Modulation of epithelial morphogenesis and cell fate by cell-to-cell signals and regulated cell adhesion.
J. Schmidt (1993)
Role of the Elb 19,000 kD tumor antigen in regulating early gene expression
E. White (1988)
10.1083/JCB.120.3.577
Signal transduction from the extracellular matrix
R. Juliano (1993)
Dynamics of membrane-cytuskeletun
W. Nelson (1986)
Activation-driven T cell death
D. Ucker (1992)
10.1016/S0959-437X(05)80342-9
DNA tumor virus transforming proteins and the cell cycle.
E. Moran (1993)
10.1016/0092-8674(92)90440-N
Epithelial morphogenesis
B. Gumbiner (1992)
Role of Elb proteins in transformation: altered organization of intermediate filaments in transformed cells that express the 19 kD protein
E. White (1990)
Glucocorticoid-induced thymocyte apoptusis is associated
A. Wyllie (1980)
Improved Retroviral Vectors for Gene Transfer and Expression
Miller Ad (1989)
Regulation of development and differentiation by the extracellular matrix.
J. Adams (1993)
10.1083/JCB.112.5.781
Differentiation requires continuous regulation
H. Blau (1991)
The 19 kD Elb transforming protein inhibits programmed cell death and prevents cytolysis by tumor necrosis factor-alpha
E. White (1992)
10.1038/327239A0
Scatter factor is a fibroblast-derived modulator of epithelial cell mobility
M. Stoker (1987)
10.1111/J.1432-0436.1989.TB00812.X
Density-dependent modulation of synthesis of keratins 1 and 10 in the human keratinocyte line HACAT and in ras-transfected tumorigenic clones.
C. Ryle (1989)
Tumorigenicity of the met protu-oncogene and the gene for hepatucyte growth factor
S. Rong (1992)
10.1016/0092-8674(91)90115-F
Induction of epithelial tubular morphogenesis in vitro by fibroblast-derived soluble factors
R. Montesano (1991)
New retrovirai vectors for gene transfer and expression
A. Miller (1989)
10.1083/JCB.124.1.1
Bcl-2 and the regulation of programmed cell death
J. C. Reed (1994)
10.1083/JCB.108.3.903
Type I collagen gel induces Madin-Darby canine kidney cells to become fusiform in shape and lose apical-basal polarity
A. Żuk (1989)
10.1128/JVI.62.9.3445-3454.1988
Role of the adenovirus E1B 19,000-dalton tumor antigen in regulating early gene expression.
E. White (1988)
10.1038/356397A0
Social controls on cell survival and cell death
M. Raff (1992)
10.1083/JCB.111.3.1047
Lamins A and C bind and assemble at the surface of mitotic chromosomes
J. R. Glass (1990)
Stabilization of the p53 tumor suppressor is induced by Ela and accompanies apoptosis
S. Lowe (1993)
Role of Elb proteins
E. White (1990)
Type I collagen Gel induces MDCK
A. Zuk (1989)
10.1073/PNAS.80.20.6361
Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.
R. C. Duke (1983)
10.1002/IJC.2910030517
Anchorage and growth regulation in normal and virus‐transformed cells
M. Stoker (1968)



This paper is referenced by
10.2307/3593107
Hydrogen Peroxide Induces Apoptotic-Like Cell Death in Coelomocytes of Themiste petricola (Sipuncula)
G. Blanco (2005)
Functional Characterisation of Glycosyltransferase 1 Domain Containing 1 gene in Mammary Carcinoma Cells
B. Shastri (2013)
10.1016/0304-3940(94)11159-G
Interaction of beta-amyloid peptides with integrins in a human nerve cell line
S. Sabo (1995)
10.1111/j.1600-065X.1994.tb00893.x
Physiological T‐Cell Death: Susceptibility is Modulated by Activation, Aging, and Transformation, but the Mechanism is Constant
D. Ucker (1994)
10.1007/978-94-011-5022-4_29
Integrin Expression and Macrophage Resistance to Apoptosis in Atherosclerosis
R. Judware (1998)
10.1002/sctm.16-0470
Immature Midbrain Dopaminergic Neurons Derived from Floor‐Plate Method Improve Cell Transplantation Therapy Efficacy for Parkinson's Disease
L. Qiu (2017)
Phosphoinositide 3-kinase regulation of anchorage-independent growth and drug resistance in small cell lung cancer cells
S. M. Moore (1999)
10.13023/ETD.2020.187
Prostate Cancer Resistance to Cabazitaxel Chemotherapy
Diane E Begemann (2020)
10.1634/stemcells.2006-0165
Survivin Identifies Keratinocyte Stem Cells and Is Downregulated by Anti‐β1 Integrin During Anoikis
A. Marconi (2007)
10.1002/path.949
Effects of src kinase and TGFβ1 on the differentiation and morphogenesis of MDCK cells grown in three‐dimensional collagen and Matrigel environments †
M. Rahikkala (2001)
10.1161/01.RES.78.1.38
Inhibition of matrix metalloproteinase activity inhibits smooth muscle cell migration but not neointimal thickening after arterial injury.
M. Bendeck (1996)
10.1016/B978-044482529-2/50026-3
Mechanisms of developmental cell death
A. Messina (1996)
10.1007/978-1-4757-4064-6_11
Post-Ligand Binding Events: Outside-In Signaling Through the Integrins
F. S. David (1997)
Differentiation and integrity of cardiac muscle cells are impaired in the absence of beta 1 integrin.
R. Fässler (1996)
10.1006/BBRC.1997.6593
RGD-containing peptides trigger apoptosis in glomerular mesangial cells of adult human kidneys.
X. Chen (1997)
10.1128/JVI.71.4.3168-3177.1997
Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus.
S. Chiocca (1997)
10.1002/(SICI)1097-0215(19970703)72:1<77::AID-IJC12>3.0.CO;2-7
Pleural mesothelioma mimics the integrin profile of activated, sessile rather than detached mesothelial cells
T. Barth (1997)
10.1128/MCB.21.15.5094-5108.2001
Proteolysis of the Docking Protein HEF1 and Implications for Focal Adhesion Dynamics
G. O'Neill (2001)
10.1523/JNEUROSCI.21-05-01464.2001
c-Src Is Required for Glial Cell Line-Derived Neurotrophic Factor (GDNF) Family Ligand-Mediated Neuronal Survival via a Phosphatidylinositol-3 Kinase (PI-3K)-Dependent Pathway
M. Encinas (2001)
10.1016/S0955-0674(97)80124-X
Integrins and anoikis.
S. Frisch (1997)
10.1002/dvdy.24162
Coming into focus: The role of extracellular matrix in vertebrate optic cup morphogenesis
K. Kwan (2014)
10.1023/A:1018746923474
Matrix Metalloproteinases in Remodeling of the Normal and Neoplastic Mammary Gland
L. Rudolph-Owen (2004)
10.1083/JCB.144.6.1337
Extracellular Matrix Regulates Apoptosis in Mammary Epithelium through a Control on Insulin Signaling
N. Farrelly (1999)
10.1002/1097-0215(20010601)92:5<641::AID-IJC1243>3.0.CO;2-P
αvβ6integrin promotes invasion of squamous carcinoma cells through up‐regulation of matrix metalloproteinase‐9
G. Thomas (2001)
10.1016/S0945-053X(99)00027-X
β1-Integrin–collagen interaction reduces chondrocyte apoptosis
L. Cao (1999)
10.1002/(SICI)1097-0169(199911)44:3<168::AID-CM2>3.0.CO;2-0
Attenuation of plasminogen activator inhibitor type-1 promoter activity in serum-stimulated renal epithelial cells by a distal 5' flanking region.
J. Slack (1999)
10.1007/978-1-4615-4207-0_6
Characterization and Localization of a Novel Tomato Arabinogalactan-Protein (LeAGP-1) and the Involvement of Arabinogalactan-Proteins in Programmed Cell Death
A. Showalter (2000)
10.1378/CHEST.117.3.684
Upregulation of gelatinases A and B, collagenases 1 and 2, and increased parenchymal cell death in COPD.
L. Segura-Valdez (2000)
10.1016/S1357-2725(99)00043-6
Bi-directional signal transduction by integrin receptors.
M. Coppolino (2000)
10.1002/jcb.10322
Anchorage‐independent phosphorylation of p130Cas protects lung adenocarcinoma cells from anoikis
L. Wei (2002)
10.1002/jcp.10123
Recovery of cellular E‐cadherin precedes replenishment of estrogen receptor and estrogen‐dependent proliferation of breast cancer cells rescued from a death stimulus
C. Malaguti (2002)
10.1073/pnas.1015938108
Retaining cell–cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer
Jumpei Kondo (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar