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Structural Changes Accompanying GTP Hydrolysis In Microtubules: Information From A Slowly Hydrolyzable Analogue Guanylyl-(alpha,beta)-methylene-diphosphonate.

A A Hyman, D Chrétien, I Arnal, R H Wade

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We have used cryoelectron microscopy to try to understand the structural basis for the role of GTP hydrolysis in destabilizing the microtubule lattice. We have measured a structural difference introduced into microtubules by replacing GTP with guanylyl-(alpha,beta)-methylene-diphosphonate (GMPCPP). In a stable GMPCPP microtubule lattice, the moiré patterns change and the tubulin subunits increase in size by 1.5 A. This information provides a clue to the role of hydrolysis in inducing the structural change at the end of a microtubule during the transition from a growing to a shrinking phase.