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Rat Monoclonal Antitubulin Antibodies Derived By Using A New Nonsecreting Rat Cell Line

J. Kilmartin, B. Wright, C. Milstein
Published 1982 · Medicine, Biology

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Hybrid myeloma cell lines secreting monoclonal antibodies to tubulin have been prepared using rat myelomas and spleen cells from rats immunized with yeast tubulin. A comparison between the results obtained with the rat myeloma Y3-Ag 1.2.3., which secretes a light chain, and a new line, YB2/O, which does not, shows that they are both excellent parental lines and that the second produces hybrids with no myeloma chain components. The antitubulin antibodies in the serum of rats bearing two of the hybrid myeloma tumors gave titers of up to 1:10(6) from which large amounts of monoclonal antibodies could be easily purified. They recognized tubulin from yeast as well as from birds and mammals. The two antibodies gave clear immunofluorescent staining of yeast mitotic spindles as well as the interphase microtubule network of tissue culture cells. Some difference in the pattern of immunofluorescence staining of yeast cells and nuclei was observed between the two antibodies. The purified antibodies could be conjugated to colloidal gold particles and used for direct labeling of yeast microtubules for electron microscopy.
This paper references
10.1016/S0074-7696(08)61757-X
Microtubules in cultured cells; indirect immunofluorescent staining with tubulin antibody.
B. Brinkley (1980)
10.1083/JCB.53.3.837
MEIOTIC SPINDLE PLAQUES IN SACCHAROMYCES CEREVISIAE
J. B. Peterson (1972)
Radioimmunoassay for tubulin detection. FEBS (Fed Eur
Le Guem (1977)
10.1073/PNAS.75.1.334
Combined immunofluorescence and high-voltage electron microscopy of cultured mammalian cells, using an antibody that binds to glutaraldehyde-treated tubulin.
B. S. Eckert (1978)
10.1016/s0021-9258(19)42621-5
Purification and properties of flagellar outer doublet tubulin from Naegleria gruberi and a radioimmune assay for tubulin.
J. D. Kowit (1974)
10.1073/PNAS.78.2.1037
Calcium lability of cytoplasmic microtubules and its modulation by microtubule-associated proteins.
M. Schliwa (1981)
Presenc e of NADPH-cytochrome P_ reductase in rat liver Golgi membranes . Evidence obtained by immunoadsorption methods
A Ito (1978)
Monoclonal antibodies directed against yeast tubulin: cross-reaction to tubulin from pig and Physamm polycephalum and subunit specificity
M T Chang (1981)
Immunocytochemical evidence for the association of calmodulin with microtubules of the mitotic apparatus
J De May (1980)
10.1007/978-1-4684-2841-4_3
Differentiation antigens of the lymphocyte cell surface.
A. F. Williams (1977)
10.1083/JCB.77.3.R27
Individual microtubules viewed by immunofluorescence and electron microscopy in the same PtK2 cell
M. Osborn (1978)
Multiple a and 0 tubulin genes in Chlamydomonas and regulation of tubulin mRNA levels after flagellation
C D Silflow (1981)
10.1073/PNAS.73.3.867
Cytoplasmic microtubules in tissue culture cells appear to grow from an organizing structure towards the plasma membrane.
M. Osborn (1976)
10.1021/BI00631A004
Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules.
D. Murphy (1977)
Fusion of two immunoglobulin
C. Milstein (1973)
10.1038/277131a0
Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG
G. Galfrè (1979)
10.1016/0926-6550(64)90174-4
STUDIES ON THE YEAST NUCLEUS. I. THE ISOLATION OF NUCLEI.
T. H. Rozijn (1964)
Three rat monoclonal antibodies to human C3.
P. Lachmann (1980)
10.1016/0309-1651(81)90204-6
High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method.
J. D. De Mey (1981)
Preparation of monoclonal antibodies: strategies and procedures.
G. Galfrè (1981)
10.1021/BI00515A050
Purification of yeast tubulin by self-assembly in vitro.
J. Kilmartin (1981)
Monoclonal antibodies directed against yeast tubulin: cross-reaction to tubulin from pig and Physamm polycephalum and subunit specificity
V . Kilmartin (1981)
Immunofluorescence of the tubulin system in cellular slime molds. FEBS (Fed
E Unger (1979)
10.1126/SCIENCE.1096300
Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.
G. Fuller (1975)
10.1038/244042A0
Fusion of Two Immunoglobulin-producing Myeloma Cells
R. Cotton (1973)
Radioimmunoassay for tubuhn: a quantitative comparison of the tubulin content of different established tissue culture cell lines and tissues
Hiller (1978)
Insmunochemical determination of tubulin. FEBS (Fed. Eur
I Gozw (1977)
Purification and properties of flagellar outer doublet
J. D. Kowit (1974)
10.1016/s0021-9258(19)40863-6
Membrane-bound tubulin in brain and thyroid tissue.
B. Bhattacharyya (1975)
Insmunochemical determination of tubulin
I Gozw (1977)
Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
H. Bazin (1974)
Quantitation and characterization of antibody binding to tubulin.
L. Van de Water (1978)
10.1083/JCB.65.1.237
Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules
WL Dentler (1975)
10.1101/SQB.1974.038.01.016
Duplication of spindle plaques and integration of the yeast cell cycle.
B. Byers (1974)
Quantitation and characterization
Van De Water (1978)
10.1002/EJI.1830060713
Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusion
G. Köhler (1976)
10.1007/BF01538755
Expression of spleen cell immunoglobulin phenotype in hybrids with myeloma cell lines
M. Clark (1981)
Immunocytochemical visualization of microtubules and tubulin at the light- and electron-microscopic level.
M. De Brabander (1977)
10.1073/PNAS.75.3.1414
Species-dependent immunological differences between vertebrate brain tubulins.
J. L. Morgan (1978)
10.1073/PNAS.75.4.1820
Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy.
K. Weber (1978)
10.1016/0022-2836(76)90293-X
Griseofulvin interacts with microtubules both in vivo and in vitro.
K. Weber (1976)



This paper is referenced by
10.1006/cbir.1996.0103
PRESENCE OF MULTIPLE CENTRIOLES AND PRIMARY CILIA DURING GROWTH AND EARLY DIFFERENTIATION IN THE MYOBLAST CO25 CELL LINE
M. Zeytinoglu (1996)
10.1016/J.INTIMP.2007.01.014
Quantitative in vivo comparisons of the Fc gamma receptor-dependent agonist activities of different fucosylation variants of an immunoglobulin G antibody.
Bernard J Scallon (2007)
10.1016/0014-5793(91)80613-8
Rapid purification and characterisation of HIV‐1 reverse transcriptase and RNaseH engineered to incorporate a C‐terminal tripeptide α‐tubulin epitope
D. Stammers (1991)
10.1083/JCB.112.1.111
Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy
M. De Brabander (1991)
oronin 1 C-free primary mouse fibroblasts exhibit robust earrangements in the orientation of actin filaments , microtubules nd intermediate filaments
uliane Behrensa (2016)
10.1016/S0960-9822(02)00820-5
The Aurora B Kinase AIR-2 Regulates Kinetochores during Mitosis and Is Required for Separation of Homologous Chromosomes during Meiosis
Susanne Kaitna (2002)
10.1016/0092-8674(84)90055-2
Targeting of E. coli β-galactosidase to the nucleus in yeast
M. Hall (1984)
10.1007/978-1-4684-4964-8_1
Monoclonal Antibody Strategy and Techniques
Z. Eshhar (1985)
10.1016/0014-4827(84)90367-7
Indirect immunofluorescence of microtubules in Dictyostelium discoideum. A study with polyclonal and monoclonal antibodies to tubulins.
U. Roos (1984)
10.1002/CM.970100110
Results obtained with a sensitive confocal scanning system designed for epifluorescence.
W. Amos (1988)
10.1007/BF00533400
Immunohistochemical heterogeneity of alpha-tubulin in human epithelia revealed with monoclonal antibodies
P. Dráber (2004)
10.1007/978-1-4612-4826-2_1
Current Methodologies in Hybridoma Formation
A. Bartal (1987)
10.1523/JNEUROSCI.22-22-09733.2002
Tau-Mediated Cytotoxicity in a Pseudohyperphosphorylation Model of Alzheimer's Disease
Thomas Fath (2002)
Actin filament bundles are required for microtubule reorientation during growth cone turning to avoid an inhibitory guidance cue.
J. F. Challacombe (1996)
The budding yeast Msh4 protein functions in chromosome synapsis and the regulation of crossover distribution.
J. E. Novak (2001)
10.1007/BF02737896
Cell contraction caused by microtubule disruption is accompanied by shape changes and an increased elasticity measured by scanning acoustic microscopy
I. Karl (2007)
10.1016/B978-0-12-668330-1.50017-X
CHAPTER 13 – Spatial Organization of Microtubules in PC12 Cells: Three-Dimensional Electron Microscopy and Confocal Microscopy Applied to Volume Investigation
J. Trogadis (1994)
10.1083/JCB.119.4.867
Newly assembled microtubules are concentrated in the proximal and distal regions of growing axons
A. Brown (1992)
10.1016/S0932-4739(11)80331-7
Dynamics of the cytoskeleton during morphogenesis in the ciliate Euplotes I. Basal bodies related microtubular system.
A. Fleury (1991)
10.1007/BF00492606
Heterogeneity of tubulin epitopes in mouse fetal tissues
P. Dráber (2004)
10.1016/0196-9781(88)90072-1
Endooligopeptidase A activity in rabbit heart: Generation of enkephalin from enkephalin containing peptides
M. Cicilini (1988)
10.1016/0022-1759(87)90147-5
Hybridoma technology: new developments of practical interest
S. Samoilovich (1987)
10.1038/onc.2017.45
The drebrin/EB3 pathway drives invasive activity in prostate cancer
A. E. Dart (2017)
10.1016/j.ajhg.2012.03.016
A truncating mutation of CEP135 causes primary microcephaly and disturbed centrosomal function.
M. S. Hussain (2012)
Large-scale analysis of gene expression, protein localization, and gene dlsrupuon in Saccharomyces cerevisiae
N. Burns (2007)
10.3390/cells9061482
A Microfluidic Single-Cell Cloning (SCC) Device for the Generation of Monoclonal Cells
C. Yeh (2020)
10.1242/jcs.01697
Glycogen synthase kinase-3β phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons
N. Trivedi (2005)
10.1083/JCB.96.4.979
Centriole as microtubule-organizing centers for marginal bands of molluscan erythrocytes
I. Nemhauser (1983)
Microtubules facilitate the stimulated secretion of beta-hexosaminidase in lacrimal acinar cells.
S. R. da Costa (1998)
10.1016/0303-2647(88)90021-4
Probing protistan phylogenies with an anti-tubulin antibody.
G. Brugerolle (1988)
10.1007/BF01281812
Acetylation of α-tubulin in male meiotic spindles ofPyrrhocoris apterus, an insect with holokinetic chromosomes
K. Wolf (2005)
10.1007/BF01287616
Indirect immunofluorescent staining of the microtubules in interphase and mitotic amoebae of the myxomycetePhysarum polycephalum
A. Lemoine (2005)
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