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In Vivo Detection Of Dendritic Cell Antigen Presentation To CD4+ T Cells

E. Ingulli, A. Mondino, A. Khoruts, M. Jenkins
Published 1997 · Biology, Medicine

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Although lymphoid dendritic cells (DC) are thought to play an essential role in T cell activation, the initial physical interaction between antigen-bearing DC and antigen-specific T cells has never been directly observed in vivo under conditions where the specificity of the responding T cells for the relevant antigen could be unambiguously assessed. We used confocal microscopy to track the in vivo location of fluorescent dye-labeled DC and naive TCR transgenic CD4+ T cells specific for an OVA peptide–I-Ad complex after adoptive transfer into syngeneic recipients. DC that were not exposed to the OVA peptide, homed to the paracortical regions of the lymph nodes but did not interact with the OVA peptide-specific T cells. In contrast, the OVA peptide-specific T cells formed large clusters around paracortical DC that were pulsed in vitro with the OVA peptide before injection. Interactions were also observed between paracortical DC of the recipient and OVA peptide-specific T cells after administration of intact OVA. Injection of OVA peptide-pulsed DC caused the specific T cells to produce IL-2 in vivo, proliferate, and differentiate into effector cells capable of causing a delayed-type hypersensitivity reaction. Surprisingly, by 48 h after injection, OVA peptide-pulsed, but not unpulsed DC disappeared from the lymph nodes of mice that contained the transferred TCR transgenic population. These results demonstrate that antigen-bearing DC directly interact with naive antigen-specific T cells within the T cell–rich regions of lymph nodes. This interaction results in T cell activation and disappearance of the DC.
This paper references
Accessory cell-T lymphocyte interactions. Antigen-dependent and - independent clustering
K. Inaba (1986)
T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1
Michael Loran Dustin (1989)
Visualization of peptide-specific T cell immunity and peripheral tolerance induction in vivo.
E. Kearney (1994)
Bone marrow-derived dendritic cells pulsed with synthetic tumour peptides elicit protective and therapeutic antitumor immunity
J I Mayordomo (1995)
T cell receptor crosslinking transiently stimulates adhesiveness through LFA-1. Nature
M L Dustin (1989)
Clustering of dendritic cells, helper T lymphocytes, and histocompatible B cells during primary antibody responses in vitro
K. Inaba (1984)
Memory T cell development in the absence of specific antigen priming.
W. Lee (1996)
Sustained signaling leading to T cell activation results from prolonged T cell receptor occupancy. Role of T cell actin cytoskeleton
S. Valitutti (1995)
Role of dendritic cells in the priming of CD4+ T lymphocytes to peptide antigen in vivo.
D. Levin (1993)
Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo.
K. Murphy (1990)
Enrichment of dendritic cells by plastic adherence and EA rosetting
W. J. Swiggard (1991)
Antigen recognition by H-2-restricted T cells. II. A tryptic ovalbumin peptide that substitutes for processed antigen.
R. Shimonkevitz (1984)
CD95 (Fas)-dependent elimination of self-reactive B cells upon interaction with CD4+T cells
J. Rathmell (1995)
Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations
P. Openshaw (1995)
Dendritic cells and immune-based therapies.
Steinman Rm (1996)
Antigen-specific T lymphocytes efficiently cluster with dendritic cells in the human primary mixed-leukocyte reaction.
E. R. Flechner (1988)
CD40 and its ligand in host defense.
R. Noelle (1996)
Bone marrow-derived dendritic cells pulsed with synthetic tumour peptides elicit protective and therapeutic antitumor immunity
Mayordomo J.I (1995)
A Novel Migration Pathway for Rat Dendritic Cells from the Blood: Hepatic Sinusoids–Lymph Translocation
S. Kudo (1997)
Dendritic cells and immune-based therapies.
R. Steinman (1996)
Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitations.
T. Brelje (1993)
Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor
K. Inaba (1992)
T cell receptor cross - linking transiently stimulates adhesiveness through LFA - 1 . Nature ( Lond . )
S. Valitutti (1989)
Bone marrow-derived dendritic cells pulsed with synthetic tumour peptides elicit protective and therapeutic antitumour immunity
J. Mayordomo (1995)
Regulation of dendritic cell numbers and maturation by lipopolysaccharide in vivo
T. De Smedt (1996)
The dendritic cell system and its role in immunogenicity.
R. Steinman (1991)
Current protocols in immunology
E. J. Coligan (1991)
Cell-cell interactions in the T cell proliferative response
H. Y. Tse (1980)
Accessory cell–T lymphocyte interactions
K. Inaba (1986)
The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody
K. Haskins (1983)
Clustering with dendritic cells precedes and is essential for T-cell proliferation in a mitogenesis model.
J. Austyn (1988)
Flow cytometric determination of cytokines in activated murine T helper lymphocytes: Expression of interleukin‐10 in interferon‐γ and in interleukin‐4‐expressing cells
M. Assenmacher (1994)
Evidence of a role for TNF-alpha in cytolysis by CD4+, class II MHC-restricted cytotoxic T cells.
J. Tite (1990)
The MHC-restricted antigen receptor on T cells. I. Isolation of a monoclonal antibody
K. Haskins (1983)
Viral Superantigen Drives Extrafollicular and Follicular B Cell Differentiation Leading to Virus-specific Antibody Production
S. A. Luther (1997)

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J. Sun (1999)
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Y. Wei (2011)
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B. Pajak (2003)
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