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Bio-functionalization Of Magnetite Nanoparticles Using An Aminophosphonic Acid Coupling Agent: New, Ultradispersed, Iron-oxide Folate Nanoconjugates For Cancer-specific Targeting.

Manasmita Das, Debasish Mishra, Tapas Kumar Maiti, A Basak, Panchanan Pramanik
Published 2008 · Medicine, Materials Science
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The present study describes a systematic approach towards the design and development of novel, bio-functionalized, magneto-fluorescent nanoparticles for cancer-specific targeting. Biocompatible, hydrophilic, magneto-fluorescent nanoparticles with surface-pendant amine, carboxyl or aldehyde groups, to be later used for bio-conjugation, were designed using an aminophosphonic acid coupling agent. These magneto-fluorescent nanoparticles were further functionalized with folic acid, using diverse conjugation strategies. A series of new iron-oxide folate nanoconjugates with excellent aqueous dispersion stability and reasonably good hydrodynamic sizes under a wide range of physiological conditions were developed. These ultradispersed nanosystems were analyzed for their physicochemical properties and cancer-cell targeting ability, facilitated by surface modification with folic acid. The nanoparticle size, charge, surface chemistry, magnetic properties and colloidal stability were extensively studied using a variety of complementary techniques. Confocal microscopy, performed with folate receptor positive human cervical HeLa cancer cells, established that these non-cytotoxic iron-oxide folate nanoconjugates were effectively internalized by the target cells through receptor-mediated endocytosis. Cell-uptake behaviors of nanoparticles, studied using magnetically activated cell sorting (MACS), clearly demonstrated that cells over-expressing the human folate receptor internalized a higher level of these nanoparticle-folate conjugates than negative control cells.



This paper is referenced by
10.4161/biom.22347
Nanostructured porous Si-based nanoparticles for targeted drug delivery
Mohammad-Ali Shahbazi (2012)
10.1007/s10853-017-1141-3
Polymer-coated CoFe2O4 nanoassemblies as biocompatible magnetic nanocarriers for anticancer drug delivery
Muhammad Waheed Mushtaq (2017)
10.1517/17425247.2014.924501
Superparamagnetic iron oxide nanoparticles for delivery of therapeutic agents: opportunities and challenges
Sophie Laurent (2014)
10.1039/c3nr04131j
Recent advances in surface chemistry strategies for the fabrication of functional iron oxide based magnetic nanoparticles.
Kostiantyn Turcheniuk (2013)
10.1021/bc300598z
Surface chemistry dependent "switch" regulates the trafficking and therapeutic performance of drug-loaded carbon nanotubes.
Manasmita Das (2013)
10.1088/1361-6560/aa5d46
Understanding the dynamics of superparamagnetic particles under the influence of high field gradient arrays.
L. C. Barnsley (2017)
10.1016/j.colsurfb.2013.05.033
Organophosphorous modifications of multifunctional magnetic nanowires.
Beata Kalska-Szostko (2013)
10.1039/C2JM34571D
Orthogonal biofunctionalization of magnetic nanoparticles via ``clickable'' poly(ethylene glycol) silanes: a ``universal ligand'' strategy to design stealth and target-specific nanocarriers
Manasmita Das (2012)
10.5772/46121
Size and Shape Effect on Biomedical Applications of Nanomaterials
Balaprasad Ankamwar (2012)
In vitro studies of Gum Arabic-coated Magnetic Nanoparticles with Mammalian Cell Cultures
Vera Castro (2009)
10.1088/2053-1591/AA8F70
A microwave assisted one-pot route synthesis of bimetallic PtPd alloy cubic nanocomposites and their catalytic reduction for 4-nitrophenol
Jian Zhang (2017)
10.1002/smll.201100436
Engineered multifunctional nanocarriers for cancer diagnosis and therapeutics.
Donglu Shi (2011)
10.1002/9783527610419.NTLS0171
Magnetic Nanomaterials for Environmental Applications
Marvin G Warner (2011)
10.1039/C2JM15139A
Magnetic-room temperature phosphorescent multifunctional nanocomposites as chemosensor for detection and photo-driven enzyme mimetics for degradation of 2,4,6-trinitrotoluene
Wen-sheng Zou (2012)
10.1002/smll.200901219
Biofunctionalized, phosphonate-grafted, ultrasmall iron oxide nanoparticles for combined targeted cancer therapy and multimodal imaging.
Manasmita Das (2009)
10.1039/c1cc13708e
SiO2@Fe2O3 core-shell nanoparticles for covalent immobilization and release of sparfloxacin drug.
Nadia E. A. El-Gamel (2011)
10.1016/J.CARBPOL.2011.11.033
Controlling the thickness of polymeric shell on magnetic nanoparticles loaded with doxorubicin for targeted delivery and MRI contrast agent
Sumanta Kumar Sahu (2012)
10.1088/0957-4484/20/6/065102
Bio-conjugated luminescent quantum dots of doped ZnS: a cyto-friendly system for targeted cancer imaging.
Koyakutty Manzoor (2009)
10.1016/B978-0-08-100164-6.00020-5
Functionalization of magnetic nanowires for biomedical applications
Kristina Žužek Rožman (2015)
10.1016/J.ARABJC.2017.11.002
Layered magnetite nanoparticles modification : synthesis, structure, and magnetic characterization
Urszula Klekotka (2017)
10.1007/s11051-012-0964-8
Synthesis optimization and characterization of chitosan-coated iron oxide nanoparticles produced for biomedical applications
Gozde Unsoy (2012)
10.1163/092050611X562166
In Vitro Cell Uptake of Biocompatible Magnetite/Chitosan Nanoparticles with High Magnetization: A Single-Step Synthesis Approach for In-Situ-Modified Magnetite by Amino Groups of Chitosan
Yongliang Wang (2012)
10.3762/bjnano.6.143
Thermal treatment of magnetite nanoparticles
Beata Kalska-Szostko (2015)
10.1021/CM3004062
Magnetic Block Ionomer Complexes for Potential Dual Imaging and Therapeutic Agents
Nikorn Pothayee (2012)
10.1088/0957-4484/21/12/125103
Highly biocompatible and water-dispersible, amine functionalized magnetite nanoparticles, prepared by a low temperature, air-assisted polyol process: a new platform for bio-separation and diagnostics.
Manasmita Das (2010)
10.1007/S11051-010-0140-Y
Single step surface modification of highly stable magnetic nanoparticles for purification of His-tag proteins
Sumanta Kumar Sahu (2011)
10.1039/C4TB01359J
Doxorubicin and Fe3O4 loaded albumin nanoparticles with folic acid modified dextran surface for tumor diagnosis and therapy.
Hequn Hao (2014)
10.1016/j.pep.2017.12.004
Isolation of recombinant Hepatitis B surface antigen with antibody-conjugated superparamagnetic Fe3O4/SiO2 core-shell nanoparticles.
Mehdi Mostafaei (2018)
10.1016/j.ijpharm.2017.03.071
Design and evaluation of surface functionalized superparamagneto-plasmonic nanoparticles for cancer therapeutics.
M Ravichandran (2017)
10.1016/J.MOLCATB.2011.01.001
A novel method for the immobilization of urease on phosphonate grafted iron oxide nanoparticle
Banalata Sahoo (2011)
10.1038/srep20543
Multifunctional Nanocarpets for Cancer Theranostics: Remotely Controlled Graphene Nanoheaters for Thermo-Chemosensitisation and Magnetic Resonance Imaging
Arathyram Ramachandra Kurup Sasikala (2016)
10.1007/s11051-015-3276-y
Charge effect of superparamagnetic iron oxide nanoparticles on their surface functionalization by photo-initiated chemical vapour deposition
Taraneh Javanbakht (2015)
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