Online citations, reference lists, and bibliographies.
← Back to Search

Delivery Of Proteins To Mammalian Cells Via Gold Nanoparticle Mediated Laser Transfection.

D. Heinemann, S. Kalies, M. Schomaker, W. Ertmer, H. Murua Escobar, H. Meyer, T. Ripken
Published 2014 · Medicine, Materials Science

Cite This
Download PDF
Analyze on Scholarcy
Share
Nanoparticle laser interactions are in widespread use in cell manipulation. In particular, molecular medicine needs techniques for the directed delivery of molecules into mammalian cells. Proteins are the final mediator of most cellular cascades. However, despite several methodical approaches, the efficient delivery of proteins to cells remains challenging. This paper presents a new protein transfection technique via laser scanning of cells previously incubated with gold nanoparticles. The laser-induced plasmonic effects on the gold nanoparticles cause a transient permeabilization of the cellular membrane, allowing proteins to enter the cell. Applying this technique, it was possible to deliver green fluorescent protein into mammalian cells with an efficiency of 43%, maintaining a high level of cell viability. Furthermore, a functional delivery of Caspase 3, an apoptosis mediating protein, was demonstrated and evaluated in several cellular assays. Compared to conventional protein transfection techniques such as microinjection, the methodical approach presented here enables high-throughput transfection of about 10 000 cells per second. Moreover, a well-defined point in time of delivery is guaranteed by gold nanoparticle mediated laser transfection, allowing the detailed temporal analysis of cellular pathways and protein trafficking.
This paper references
10.1038/ncomms3726
Caspase-mediated activation of Caenorhabditis elegans CED-8 promotes apoptosis and phosphatidylserine externalisation
Y. Chen (2013)
10.1038/sj.gt.3301383
Protein transduction: an alternative to genetic intervention?
K. Ford (2001)
10.1039/c0cs00018c
Biodistribution and toxicity of engineered gold nanoparticles: a review of in vitro and in vivo studies.
N. Khlebtsov (2011)
10.1038/418290a
Cell biology: Targeted transfection by femtosecond laser
Uday K. Tirlapur (2002)
10.1016/j.jconrel.2011.02.006
Selective gene transfection of individual cells in vitro with plasmonic nanobubbles.
E. Lukianova-Hleb (2011)
10.1074/JBC.M112.411371
Chromatin collapse during caspase-dependent apoptotic cell death requires DFF40/CAD-mediated 3'-OH single-strand DNA breaks
Victoria Iglesias-Guimarais (2013)
10.1002/cyto.a.10068
Staurosporine‐induced apoptosis in human cornea epithelial cells in vitro
S. Haertel (2003)
10.1007/BF00697702
A novel method of DNA transfection by laser microbeam cell surgery
M. Tsukakoshi (1984)
10.1007/s11060-004-1364-4
Protein delivery of caspase-3 induces cell death in malignant C6 glioma, primary astrocytes and immortalized and primary brain capillary endothelial cells
B. Zassler (2004)
10.1007/s10103-007-0470-x
Plasmonic photothermal therapy (PPTT) using gold nanoparticles
X. Huang (2007)
10.1002/jbio.201300056
Plasmonic laser treatment for Morpholino oligomer delivery in antisense applications.
S. Kalies (2014)
10.1007/S00216-005-0247-7
Nano-oncology: drug delivery, imaging, and sensing
N. G. Portney (2006)
10.1364/JOSAB.29.001383
Noble-metal nanoparticles and short pulses for nanomanipulations: theoretical analysis
G. Bisker (2012)
10.1083/JCB.123.1.7
Nuclear events of apoptosis in vitro in cell-free mitotic extracts: a model system for analysis of the active phase of apoptosis
Y. Lazebnik (1993)
10.1111/j.1582-4934.2002.tb00313.x
Kinetics of apoptotic markers in exogeneously induced apoptosis of EL4 cells
R. Jessel (2002)
10.1117/1.3253320
Influence of laser parameters on nanoparticle-induced membrane permeabilization.
C. Yao (2009)
10.1088/0953-8984/27/23/233103
Interactions between semiconductor nanowires and living cells.
C. Prinz (2015)
10.1074/jbc.272.12.7797
Target Protease Specificity of the Viral Serpin CrmA
Q. Zhou (1997)
10.1038/nmeth.2089
NIH Image to ImageJ: 25 years of image analysis
C. Schneider (2012)
10.1038/bjc.1972.33
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
10.1038/nbt1201-1173
A peptide carrier for the delivery of biologically active proteins into mammalian cells
M. Morris (2001)
10.1074/jbc.M112.411371
Chromatin Collapse during Caspase-dependent Apoptotic Cell Death Requires DNA Fragmentation Factor, 40-kDa Subunit-/Caspase-activated Deoxyribonuclease-mediated 3′-OH Single-strand DNA Breaks*
Victoria Iglesias-Guimarais (2013)
10.1038/nnano.2007.387
Nanocarriers as an emerging platform for cancer therapy.
D. Peer (2007)
10.1038/34112
A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD
M. Enari (1998)
10.1016/S0008-6363(99)00384-3
Morphologic and biochemical hallmarks of apoptosis.
A. Saraste (2000)
10.1371/journal.pone.0058604
Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
D. Heinemann (2013)
10.1038/nrd2591
Strategies in the design of nanoparticles for therapeutic applications
Robby A. Petros (2010)
10.1016/j.stem.2009.04.005
Generation of induced pluripotent stem cells using recombinant proteins.
H. Zhou (2009)
10.1023/A:1026497306006
Apoptotic and necrotic cell death are both induced by electroporation in HL60 human promyeloid leukaemia cells
J. Piñero (2004)
10.1016/J.JPHOTOCHEMREV.2012.01.001
Studies on the interaction of pulsed lasers with plasmonic gold nanoparticles toward light manipulation, heat management, and nanofabrication
S. Hashimoto (2012)
10.1006/EXCR.2000.4834
Apoptotic DNA fragmentation.
S. Nagata (2000)
10.1016/J.JURO.2007.09.018
Selective prostate cancer thermal ablation with laser activated gold nanoshells.
J. Stern (2008)
10.1038/284555A0
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
10.1002/jbio.201200200
Enhancement of extracellular molecule uptake in plasmonic laser perforation.
S. Kalies (2014)
10.1016/j.biomaterials.2011.11.062
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells.
J. Baumgart (2012)
10.1098/rsif.2009.0463
Single cell optical transfection
D. Stevenson (2010)
10.1134/S0003683809040176
Estimation of methods of protein delivery into mammalian cells — A comparative study by electroporation and Bioporter assay
R. Todorova (2009)
10.1039/b711486a
Modelling the optical response of gold nanoparticles.
V. Myroshnychenko (2008)



This paper is referenced by
10.1016/J.JDDST.2017.06.018
Advances on the formulation of proteins using nanotechnologies
Irene Santalices (2017)
Gellan Gum-Coated Gold Nanorods for Drug Delivery Applications
SÍLVIA CRISTINA ARAÚJO VIEIRA (2014)
10.7567/JJAP.54.08LB04
Development of a single cell electroporation method using a scanning ion conductance microscope with a theta nanopipette
Satoshi Sakurai (2015)
10.1038/s41598-018-24908-9
Gold nanoparticle-mediated laser stimulation induces a complex stress response in neuronal cells
S. Johannsmeier (2018)
10.1016/j.nano.2018.04.015
Effect of laser fluence, nanoparticle concentration and total energy input per cell on photoporation of cells.
Stefany Y Holguin (2018)
10.1002/jbio.202000017
Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods.
Cuiping Yao (2020)
10.1109/NANO.2016.7751315
3D plasmonic nanostructures for in-vitro applications in neuroscience and cell biology
Valeria Caprettini (2016)
10.1117/12.2077601
Laser transfection with gold nanoparticles: current state and new particle structures as a perspective
S. Kalies (2015)
10.1515/aot-2015-0054
Near-field optics for nanoprocessing
M. Terakawa (2016)
10.2147/IJN.S140620
Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
Cuiping Yao (2017)
10.1039/c8cs00008e
Protein delivery into cells using inorganic nanoparticle-protein supramolecular assemblies.
F. Scaletti (2018)
10.1117/12.2077604
Plasmonic cell manipulation for biomedical and screening applications
D. Heinemann (2015)
10.3390/ma9050397
Gold Nanoparticle-Mediated Delivery of Molecules into Primary Human Gingival Fibroblasts Using ns-Laser Pulses: A Pilot Study
Judith Krawinkel (2016)
10.3390/ijms20174254
Delivery of Mixed-Lineage Kinase Domain-Like Protein by Vapor Nanobubble Photoporation Induces Necroptotic-Like Cell Death in Tumor Cells
L. Van Hoecke (2019)
10.1186/s12951-015-0155-8
Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates
Judith Krawinkel (2016)
10.1080/23746149.2016.1228476
Laser-assisted photoporation: fundamentals, technological advances and applications
R. Xiong (2016)
10.1016/j.nantod.2020.100895
Harnessing nanoparticles for the efficient delivery of the CRISPR/Cas9 system
H. Rahimi (2020)
10.1016/j.jpba.2015.10.021
Development of analytical methods for functional analysis of intracellular protein using signal-responsive silica or organic nanoparticles.
Masaru Kato (2016)
10.1016/j.bmcl.2015.01.018
Conceptual and technical aspects of transfection and gene delivery.
L. Kaestner (2015)
10.3390/ijms17081295
Towards Effective Photothermal/Photodynamic Treatment Using Plasmonic Gold Nanoparticles
A. Bucharskaya (2016)
10.1371/journal.pone.0124052
Investigation of Biophysical Mechanisms in Gold Nanoparticle Mediated Laser Manipulation of Cells Using a Multimodal Holographic and Fluorescence Imaging Setup
S. Kalies (2015)
10.1038/srep22055
A novel low-power laser-mediated transfer of foreign molecules into cells
S. Yumura (2016)
10.1117/12.2222830
SERS spectroscopy, electrical recording and intracellular injection in neuronal networks with 3D plasmonic nanoantennas
Valeria Caprettini (2016)
Integration of Gap Junction Coupling in Adenosine Signalling of Endothelial Cells
Von der Naturwissenschaftlichen (2018)
10.22037/tpps.v1i3.16414
Nanotechnology and Drug Delivery: Recent Applications and Future Challenges
T. T. Moghadam (2017)
10.1038/s41598-020-68512-2
Femtosecond Plasmonic Laser Nanosurgery (fs-PLN) mediated by molecularly targeted gold nanospheres at ultra-low pulse fluences
Daniel Steven Eversole (2020)
10.1117/1.JBO.20.11.115005
Characterization of the cellular response triggered by gold nanoparticle–mediated laser manipulation
S. Kalies (2015)
10.1002/adbi.201700184
CRISPR/Cas9 Genome Editing Using Gold‐Nanoparticle‐Mediated Laserporation
Berislav Bošnjak (2018)
10.1007/978-3-319-28098-1_3
Methods of Permeabilization
G. Banfalvi (2016)
10.1007/978-3-319-28098-1
Permeability of Biological Membranes
G. Banfalvi (2016)
Semantic Scholar Logo Some data provided by SemanticScholar