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Revealing The Potential Of Squid Chitosan-based Structures For Biomedical Applications.

L. L. Reys, S. S. Silva, J. M. Oliveira, S. Caridade, J. Mano, T. Silva, R. Reis
Published 2013 · Medicine, Materials Science

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In recent years, much attention has been given to different marine organisms, namely as potential sources of valuable materials with a vast range of properties and characteristics. In this work, β-chitin was isolated from the endoskeleton of the giant squid Dosidicus gigas and further deacetylated to produce chitosan. Then, the squid chitosan was processed into membranes and scaffolds using solvent casting and freeze-drying, respectively, to assess their potential biomedical application. The developed membranes have shown to be stiffer and less hydrophobic than those obtained with commercial chitosan. On the other hand, the morphological characterization of the developed scaffolds, by SEM and micro-computed tomography, revealed that the matrices were formed with a lamellar structure. The findings also indicated that the treatment with ethanol prior to neutralization with sodium hydroxide caused the formation of larger pores and loss of some lamellar features. The in vitro cell culture study has shown that all chitosan scaffolds exhibited a non-cytotoxic effect over the mouse fibroblast-like cell line, L929 cells. Thus, chitosan produced from the endoskeletons of the giant squid Dosidicus gigas has proven to be a valuable alternative to existing commercial materials when considering its use as biomaterial.
This paper references
10.1002/MABI.200700139
Viscoelastic properties of chitosan with different hydration degrees as studied by dynamic mechanical analysis.
J. Mano (2008)
10.1002/app.1969.070130815
Estimation of the surface free energy of polymers
D. Owens (1969)
Chitin: Fulfilling a Biomaterials Promise
E. Khor (2001)
10.1590/S0104-14282001000200007
Características e propriedades de quitosanas purificadas nas formas neutra, acetato e cloridrato
R. Signini (2001)
Applications and properties of chitosan J. Biact. Compat. Pol
Q Li (1992)
Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf Carbohydr. Polym
A ASagheerF (2009)
10.1016/S0142-9612(96)00167-6
In vitro and in vivo degradation of films of chitin and its deacetylated derivatives.
K. Tomihata (1997)
10.1021/bm800874q
Novel genipin-cross-linked chitosan/silk fibroin sponges for cartilage engineering strategies.
Simone S Silva (2008)
10.1016/J.CARBPOL.2009.01.032
Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf
F. A. Sagheer (2009)
10.1007/S10973-006-8038-8
Water effect in the thermal and molecular dynamics behavior of poly(L-lactic acid)
Ana Rita Brás (2007)
10.1016/J.BIOTECHADV.2006.03.002
Membrane processes in biotechnology: an overview.
C. Charcosset (2006)
10.1016/S1381-5148(00)00038-9
A review of chitin and chitosan applications
M. Kumar (2000)
Mechanical characterization of biomaterials Biodegradable Systems in Tissue Engineering and Regenerative Medicine
J F Mano (2005)
Dynamic mechanical analysis in polymers for medical applications Polymer Based Systems on
J F Mano (2002)
10.1021/ed029p15
The use of light scattering for determining particle size and molecular weight and shape
Max. Bender (1952)
10.1016/S0142-9612(00)00183-6
Influence of the degree of acetylation on some biological properties of chitosan films.
C. Châtelet (2001)
10.1002/(SICI)1097-0126(200004)49:4<337::AID-PI375>3.0.CO;2-B
Investigation of different natural sources of chitin: influence of the source and deacetylation process on the physicochemical characteristics of chitosan
M. Rhazi (2000)
10.1016/J.CARBPOL.2008.09.002
Fractionation and characterization of chitosan by analytical SEC and 1H NMR after semi-preparative SEC
Sophie Nguyen (2009)
Hetereogeneous N-deacetylation of squid chitin in alkaline solution Carbohydr. Polym
P Methacanon (2003)
10.1080/10717540590889781
Chitosan-Based Particles as Controlled Drug Delivery Systems
M. Prabaharan (2005)
10.1088/1748-6041/7/5/054104
Chitosan/bioactive glass nanoparticles composites for biomedical applications.
G. Luz (2012)
10.1016/j.actbio.2009.05.027
Chitosan scaffolds incorporating lysozyme into CaP coatings produced by a biomimetic route: a novel concept for tissue engineering combining a self-regulated degradation system with in situ pore formation.
Ana M. Martins (2009)
Production and characterization of chitosan fibers and 3-D fiber mesh scaffolds for tissue engineering applications Macromol
K Tuzlakoglu (2004)
10.1007/978-94-010-0305-6_10
Dynamic Mechanical Analysis in Polymers for Medical Applications
J. F. Mano (2002)
In vitro and in vivo degradation of films of chitin and its deacetylated derivatives Biomaterials
K Tomihata (1997)
Fabrication and characterization of chitosan/gelatin porous scaffolds with predefined internal microstructures Polymer
H Jiankanga (2007)
Biological evaluation of medical devices:part 5. Test for in vitro cytotoxicity ISO 10993
(2009)
10.1016/S0144-8617(02)00300-4
Heterogeneous N-deacetylation of squid chitin in alkaline solution
P. Methacanon (2003)
10.1016/J.POLYMER.2003.10.025
Contribution to the preparation of chitins and chitosans with controlled physico-chemical properties
A. Tolaimate (2003)
10.1016/J.BIOMATERIALS.2006.07.034
Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells.
J. Oliveira (2006)
10.1098/rsif.2007.0220
Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends
J. Mano (2007)
Características e propiedades de quitosanas purificadas nas formas neutra, acetato e cloridrato Polímeros: Ciência e Tecnologia
R Signini (2001)
10.1201/9780203491232.CH9
Mechanical Characterization of Biomaterials
J. F. Mano (2004)
Chitosan derivates obtained by chemical modifications for biomedical and environmental applications
N Alves (2008)
Caracterı́sticas e propiedades de quitosanas purificadas nas formas neutra, acetato e cloridrato Polı́meros
R Signini (2001)
10.1016/J.PROGPOLYMSCI.2006.06.001
Chitin and chitosan: Properties and applications
M. Rinaudo (2006)
Fractionation and characterization of chitosan by analytical SEC and 1 H NMR after semi-preparative SEC Carbohydr. Polym
S Nguyen (2009)
Low-molecular-weight chitosans derived from beta-chitin: preparation, molecular characteristics and aggregation activity Carbohydr. Polym
M Shimojoh (1998)
10.1016/S0144-8617(97)00244-0
Low-molecular-weight chitosans derived from β-chitin : preparation, molecular characteristics and aggregation activity
Manabu Shimojoh (1998)
10.1179/1743280412Y.0000000002
Materials of marine origin: a review on polymers and ceramics of biomedical interest
T. Silva (2012)
10.1016/S0142-9612(00)00377-X
Cytocompatibility and response of osteoblastic-like cells to starch-based polymers: effect of several additives and processing conditions.
M. Gomes (2001)
10.1016/J.POLYMER.2007.05.048
Fabrication and characterization of chitosan/gelatin porous scaffolds with predefined internal microstructures
He Jian-kang (2007)
Chitin and chitosan: properties and applications Prog
M Rinaudo (2006)
10.1002/MABI.200300100
Production and characterization of chitosan fibers and 3-D fiber mesh scaffolds for tissue engineering applications.
K. Tuzlakoglu (2004)
10.1021/BM034401T
New aspects of the extraction of chitin from squid pens.
Géraldine Chaussard (2004)
10.1016/j.ijbiomac.2008.09.007
Chitosan derivatives obtained by chemical modifications for biomedical and environmental applications.
N.M. Alves (2008)
Analysis of freeze-gelation and cross-liking processes for preparing porous chitosan scaffolds Carbohydr
C-Y Hsieh (2007)
Mechanical characterization of biomaterials Biodegradable Systems in Tissue Engineering and Regenerative Medicine ed
J FMano (2005)
10.1016/0142-9612(89)90066-5
N-acetylation in chitosan and the rate of its enzymic hydrolysis.
S. Hirano (1989)
Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf Carbohydr. Polym
F A A Sagheer (2009)
10.3403/30170165
Biological evaluation of medical devices
British Standard (2006)
10.1177/088391159200700406
Applications and Properties of Chitosan
Qiangliang Li (1992)
10.1016/S0142-9612(99)00011-3
Porous chitosan scaffolds for tissue engineering.
S. Madihally (1999)
10.1016/J.CARBPOL.2006.05.002
Analysis of freeze-gelation and cross-linking processes for preparing porous chitosan scaffolds
Chien-Yang Hsieh (2007)



This paper is referenced by
10.1016/j.msec.2015.05.029
A novel squid pen chitosan/hydroxyapatite/β-tricalcium phosphate composite for bone tissue engineering.
Amin Shavandi (2015)
10.1007/s00289-018-2483-y
Elaboration of hydroxyapatite nanoparticles and chitosan/hydroxyapatite composites: a present status
Djalila Boudemagh (2018)
10.1007/s10856-018-6025-9
Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes
Rita López-Cebral (2018)
10.1016/j.ijbiomac.2016.04.046
Bio-scaffolds produced from irradiated squid pen and crab chitosan with hydroxyapatite/β-tricalcium phosphate for bone-tissue engineering.
Amin Shavandi (2016)
10.1039/d0tb00483a
A decellularized scaffold derived from squid cranial cartilage for use in cartilage tissue engineering.
Thou Lim (2020)
10.1071/CH14274
Photoluminescence from Chitosan for Bio-Imaging
Xiaoyong Pan (2014)
10.1016/j.bioelechem.2015.02.004
Effective immobilization of glucose oxidase on chitosan submicron particles from gladius of Todarodes pacificus for glucose sensing.
J. R. Anusha (2015)
10.1016/j.ijbiomac.2015.07.012
Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
Amin Shavandi (2015)
10.1039/C8GC01120F
Ionic liquids for the preparation of biopolymer materials for drug/gene delivery: a review
J. Chen (2018)
10.4324/9781315313535
Industrial Applications of Marine Biopolymers
P. N. Sudha (2017)
10.1016/j.biomaterials.2014.08.020
A functional biphasic biomaterial homing mesenchymal stem cells for in vivo cartilage regeneration.
Hongjie Huang (2014)
10.1007/s10971-015-3899-6
Injectable gel from squid pen chitosan for bone tissue engineering applications
Amin Shavandi (2015)
10.1002/9781119126218.CH14
Recent Developments on Chitosan Applications in Regenerative Medicine
A. Duarte (2016)
In fl uence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan sca ff olds towards cartilage tissue engineering
L. L. Reys (2017)
10.1088/1748-6041/9/3/035008
Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering.
Hongjie Huang (2014)
Development of extraction methods to obtain high added-value products from industrial processing wastes of "Illex argentinus" and "Cynara scolimus"
Diana Noriega Rodríguez (2018)
10.1016/J.EURPOLYMJ.2017.08.017
Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering
Lara L. Reys (2017)
10.1016/j.jare.2016.08.006
Mechanochemical synthesis of chitosan submicron particles from the gladius of Todarodes pacificus
J. Anusha (2016)
10.1039/C6GC02827F
Ionic liquids in the processing and chemical modification of chitin and chitosan for biomedical applications
S. S. Silva (2017)
10.1016/j.carbpol.2013.06.022
Effect of crosslinking in chitosan/aloe vera-based membranes for biomedical applications.
S. S. Silva (2013)
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