Online citations, reference lists, and bibliographies.
← Back to Search

Generic Epitaxial Graphene Biosensors For Ultrasensitive Detection Of Cancer Risk Biomarker

Z. Tehrani, G. Burwell, M. A. M. Azmi, A. Castaing, R. Rickman, J. Almarashi, P. Dunstan, A. M. Beigi, S. Doak, O. Guy
Published 2014 · Materials Science

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
A generic electrochemical method of ?bioreceptor? antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (nM) concentrations of the cancer risk (oxidative stress) biomarker 8-hydroxydeoxyguanosine (8-OHdG). X-ray photoelectron spectroscopy, Raman spectroscopy are used to characterize the functionalized MLEG. Confocal fluorescence microscopy using fluorescent-labelled antibodies indicates that the anti-8-OHdG antibody selectively binds to the phenyl amine-functionalized MLEG?s channel. Current?voltage measurements on functionalized channels showed repeatable current responses from antibody?biomarker binding events. This technique is scalable, reliable, and capable of providing a rapid, quantitative, label-free assessment of biomarkers at nano-molar (<20 nM) concentrations in analyte solutions. The sensitivity of the sensor device was investigated using varying concentrations of 8-OHdG, with changes in the sensor?s channel resistance observed upon exposure to 8-OHdG. Detection of 8-OHdG concentrations as low as 0.1 ng ml?1 (0.35 nM) has been demonstrated. This is five times more sensitive than reported enzyme linked immunosorbent assay tests (0.5 ng ml?1).
This paper references
10.1524/zkri.1958.110.16.372
Phänomenologische Theorie der Kristallabscheidung an Oberflächen. I
E. Bauer (1958)
10.1524/zkri.1958.110.16.395
Phänomenologische Theorie der Kristallabscheidung an Oberflächen. II
E. Bauer (1958)
10.1103/PHYSREVLETT.41.1831
Azimuthal Anisotropy in Core-Level X-Ray Photoemission from c (2×2) Oxygen on Cu(001): Experiment and Single-Scattering Theory
S. Kono (1978)
10.1021/JA00040A074
Covalent Modification of Carbon Surfaces by Grafting of Functionalized Aryl Radicals Produced from Electrochemical Reduction of Diazonium Salts
M. Delamar (1992)
10.1016/S0014-5793(97)00523-1
Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8‐hydroxy‐2′‐deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment
M. Erhola (1997)
10.1021/JA010462S
Functionalization of carbon nanotubes by electrochemical reduction of aryl diazonium salts: a bucky paper electrode.
J. Bahr (2001)
10.1021/JA003276F
Covalent modification of iron surfaces by electrochemical reduction of aryldiazonium salts.
A. Adenier (2001)
10.1016/S0009-8981(03)00191-8
Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers.
C. Chiou (2003)
10.1016/J.CCCN.2003.09.010
Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics.
L. Wu (2004)
10.1039/B406228K
Attachment of organic layers to conductive or semiconductive surfaces by reduction of diazonium salts.
J. Pinson (2005)
10.1016/J.CARBON.2006.09.027
Hydrogen pairing on graphene
T. Roman (2007)
10.1021/LA701655W
An electrochemical and XPS study of reduction of nitrophenyl films covalently grafted to planar carbon surfaces.
S. Yu (2007)
10.1021/JP067256X
Functionalizing Single-Walled Carbon Nanotube Networks: Effect on Electrical and Electrochemical Properties
I. Dumitrescu (2007)
10.1016/J.JMHG.2007.05.009
Prostate cancer screening markers
A. Stangelberger (2007)
10.1038/nmat1967
Detection of individual gas molecules adsorbed on graphene.
F. Schedin (2007)
10.1063/1.2828975
Few-layer graphene on SiC, pyrolitic graphite, and graphene: A Raman scattering study
C. Faugeras (2007)
10.1002/JRS.1792
Self‐assembled silver nanoparticle monolayer on glassy carbon: an approach to SERS substrate
Hongjun Chen (2007)
10.1021/nl802156w
Raman spectra of epitaxial graphene on SiC and of epitaxial graphene transferred to SiO2.
Dong Su Lee (2008)
10.1021/nl802412n
Graphene-based single-bacterium resolution biodevice and DNA transistor: interfacing graphene derivatives with nanoscale and microscale biocomponents.
N. Mohanty (2008)
10.1038/nnano.2007.432
Graphene: calling all chemists.
R. Ruoff (2008)
10.1103/PHYSREVLETT.100.125504
Why multilayer graphene on 4H-SiC(0001[over ]) behaves like a single sheet of graphene.
J. Hass (2008)
10.1021/ja806499w
Diazonium functionalization of surfactant-wrapped chemically converted graphene sheets.
Jay R. Lomeda (2008)
10.1007/S11664-009-0803-6
Thickness Estimation of Epitaxial Graphene on SiC Using Attenuation of Substrate Raman Intensity
S. Shivaraman (2009)
10.1038/nature07719
Large-scale pattern growth of graphene films for stretchable transparent electrodes
K. Kim (2009)
10.1021/ja8057327
Chemical modification of epitaxial graphene: spontaneous grafting of aryl groups.
E. Bekyarova (2009)
10.1103/PHYSREVB.79.125411
Insights into few-layer epitaxial graphene growth on 4H-SiC(000(1)over-bar substrates from STM studies
L. Biedermann (2009)
10.1002/jcb.22227
Biomarkers for prostate cancer
E. Leman (2009)
10.1080/10590500902885684
8-hydroxy-2′ -deoxyguanosine (8-OHdG): A Critical Biomarker of Oxidative Stress and Carcinogenesis
A. Valavanidis (2009)
10.1126/science.1167130
Control of Graphene's Properties by Reversible Hydrogenation: Evidence for Graphane
D. Elias (2009)
10.4028/www.scientific.net/MSF.615-617.223
Investigation of Graphene Growth on 4H-SiC
A. Castaing (2009)
Investigation of Graphene
A. Castaing (2009)
Getzenberg , & Alan W . Partin , Biomarkers for Prostate Cancer
Stacy Loeb (2009)
10.1021/la101254k
Spatially resolved spontaneous reactivity of diazonium salt on edge and basal plane of graphene without surfactant and its doping effect.
Hyunseob Lim (2010)
10.1038/nmat2710
Bandgap opening in graphene induced by patterned hydrogen adsorption.
R. Balog (2010)
10.1021/nn901899j
Kinetics of diazonium functionalization of chemically converted graphene nanoribbons.
Alexander Sinitskii (2010)
10.1002/CHIN.201005256
Chemical Functionalization of Graphene
D. W. Boukhvalov (2010)
10.1021/nn901587x
Continuous, highly flexible, and transparent graphene films by chemical vapor deposition for organic photovoltaics.
Lewis Gomez de Arco (2010)
10.1021/nn100315s
Nitrogen-doped graphene and its application in electrochemical biosensing.
Ying Wang (2010)
10.1016/J.ELECTACTA.2010.06.089
Covalent modification of glassy carbon electrode by Nile blue: Preparation, electrochemistry and electrocatalysis
Zahra Nazemi (2010)
10.1002/CHIN.201024271
Carbon Nanomaterials in Biosensors: Should You Use Nanotubes or Graphene?
W. Yang (2010)
10.1039/B920539J
The chemistry of graphene
K. Loh (2010)
10.1126/science.1188119
Nanoscale Tunable Reduction of Graphene Oxide for Graphene Electronics
Zhongqing Wei (2010)
10.1088/0022-3727/43/37/374011
Current status of self-organized epitaxial graphene ribbons on the C face of 6H–SiC substrates
N. Camara (2010)
10.1038/nature09405
High-speed graphene transistors with a self-aligned nanowire gate
L. Liao (2010)
10.1126/science.327.5966.734-b
100 GHz Transistors from Wafer Scale Epitaxial Graphene
Yuming Lin (2010)
10.1016/J.TRAC.2010.05.011
Graphene for electrochemical sensing and biosensing
M. Pumera (2010)
10.1021/nl902741x
Anomalously large reactivity of single graphene layers and edges toward electron transfer chemistries.
R. Sharma (2010)
10.1016/J.CAP.2009.12.024
Graphene-based nitrogen dioxide gas sensors
Geunwoo Ko (2010)
10.1038/nature09979
High-frequency, scaled graphene transistors on diamond-like carbon
Y. Wu (2011)
10.1021/nn2023232
Graphene covalently binding aryl groups: conductivity increases rather than decreases.
P. Huang (2011)
10.1038/nchem.1010
Covalent bulk functionalization of graphene.
J. Englert (2011)
10.1021/nn300757t
In situ intercalation replacement and selective functionalization of graphene nanoribbon stacks.
Boštjan Genorio (2012)
10.1038/nchem.1421
Understanding and controlling the substrate effect on graphene electron-transfer chemistry via reactivity imprint lithography.
Q. Wang (2012)
10.1126/science.1220527
Graphene Barristor, a Triode Device with a Gate-Controlled Schottky Barrier
Heejun Yang (2012)
10.1039/c1cs15193b
Chemistry and physics of a single atomic layer: strategies and challenges for functionalization of graphene and graphene-based materials.
L. Yan (2012)
10.1088/0034-4885/76/4/046601
The self-assembly, aggregation and phase transitions of food protein systems in one, two and three dimensions.
R. Mezzenga (2013)
10.1021/nl304632e
Disorder imposed limits of mono- and bilayer graphene electronic modification using covalent chemistry.
Chih-Jen Shih (2013)
10.1021/nl304734g
The role of external defects in chemical sensing of graphene field-effect transistors.
B. Kumar (2013)
10.1021/AR300125W
Organic synthesis on graphene.
F. M. Koehler (2013)



This paper is referenced by
10.3390/ma14030590
Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
A. Lebedev (2021)
10.1007/s00604-020-04689-7
Nanostructured material–based electrochemical sensing of oxidative DNA damage biomarkers 8-oxoguanine and 8-oxodeoxyguanosine: a comprehensive review
A. Chiorcea-Paquim (2021)
10.1016/j.carbon.2020.10.048
Graphene-based field effect transistor biosensors for breast cancer detection: A review on biosensing strategies
I. Novodchuk (2021)
10.1039/D0NA00801J
Graphene based electrochemical immunosensor for the ultra-sensitive label free detection of Alzheimer's beta amyloid peptides Aβ(1–42)
H. Abbasi (2021)
10.1126/sciadv.abf0812
Real-time imaging of Na+ reversible intercalation in “Janus” graphene stacks for battery applications
Jinhua Sun (2021)
10.3390/nano11020416
Assessing Surface Coverage of Aminophenyl Bonding Sites on Diazotised Glassy Carbon Electrodes for Optimised Electrochemical Biosensor Performance
Z. Tehrani (2021)
10.3390/nano11082121
Application of Molecular Vapour Deposited Al2O3 for Graphene-Based Biosensor Passivation and Improvements in Graphene Device Homogeneity
Muhammad Munem Ali (2021)
10.1007/978-981-32-9898-9_15
Nanodevices: The Future of Medical Diagnostics
A. Mukherjee (2020)
10.1063/5.0022341
Electronic interface and charge carrier density in epitaxial graphene on silicon carbide. A review on metal–graphene contacts and electrical gating
S. Aslanidou (2020)
10.1016/j.diamond.2020.107969
Nanoscale profiling of multilayer graphene films on silicon carbide by a focused ion beam
A. Kolomiytsev (2020)
10.1063/5.0028108
Detection of lysine molecular ions in solution gated field effect transistors based on unmodified graphene
A. Butko (2020)
10.1088/2053-1583/ab72d5
Printable and flexible graphene pH sensors utilising thin film melanin for physiological applications
Z. Tehrani (2020)
10.1134/s1063785020050296
Studying the Sensitivity of Graphene for Biosensor Applications
A. Usikov (2020)
10.3390/nano10091808
A Facile Method for the Non-Covalent Amine Functionalization of Carbon-Based Surfaces for Use in Biosensor Development
Ffion Walters (2020)
10.1007/s11706-020-0530-8
A review on graphene-based materials as versatile cancer biomarker sensors
S. Basu (2020)
10.1021/acsomega.9b03821
Selective Detection of Cu2+ Ions by Immobilizing Thiacalix[4]arene on Graphene Field-Effect Transistors
Yuki Takagiri (2020)
10.3390/coatings10101008
The Effect of Carbon Ink Rheology on Ink Separation Mechanisms in Screen-Printing
Sarah-Jane Potts (2020)
10.1038/s41598-019-43680-y
Controlling parameters and characteristics of electrochemical biosensors for enhanced detection of 8-hydroxy-2′-deoxyguanosine
A. M. Faria (2019)
Graphene stacked junction diode for terahertz emission
都 継瑶 (2019)
10.1007/s11998-019-00291-6
High-speed imaging the effect of snap-off distance and squeegee speed on the ink transfer mechanism of screen-printed carbon pastes
Sarah-Jane Potts (2019)
10.1016/J.MSSP.2019.02.039
Raman probing of hydrogen-intercalated graphene on Si-face 4H-SiC
I. Shtepliuk (2019)
10.1038/s41598-019-50412-9
Chemically Functionalised Graphene FET Biosensor for the Label-free Sensing of Exosomes
Deana Kwong Hong Tsang (2019)
10.1002/9781119468455.ch110
Finite Element Analysis of Graphene Materials
Androniki S. Tsiamaki (2019)
10.1016/J.APCATB.2018.09.098
Efficient visible light driven hydrogen generation using 9-(3,3-dimethyl-1,2-oxazetidine-N-yl)perylene-3,4-dicarboximide functionalized amino graphene
A. Kalyanasundaram (2019)
10.1088/1361-6528/ab134e
Probing the uniformity of hydrogen intercalation in quasi-free-standing epitaxial graphene on SiC by micro-Raman mapping and conductive atomic force microscopy.
F. Giannazzo (2019)
10.3176/PROC.2019.2.13
Graphene/SiC dies for electrochemical blood-type sensing
A. Usikov (2019)
10.3390/bios9010016
Ultrathin Functional Polymer Modified Graphene for Enhanced Enzymatic Electrochemical Sensing
A. Devadoss (2019)
10.1007/978-981-32-9057-0_13
Graphene Functionalizations on Copper by Spectroscopic Techniques
M. Gülcan (2019)
10.4028/www.scientific.net/KEM.799.197
Electrochemical Treatment of Graphene
A. Usikov (2019)
10.4028/www.scientific.net/MSF.924.909
Graphene/SiC Functionalization for Blood Type Sensing Applications
S. Lebedev (2018)
10.1007/978-3-319-69378-1_9
CNT Applications in Sensors and Actuators
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_32
Structural Aspects and Morphology of CPs
P. Chandrasekhar (2018)
See more
Semantic Scholar Logo Some data provided by SemanticScholar