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Generic Epitaxial Graphene Biosensors For Ultrasensitive Detection Of Cancer Risk Biomarker
Z. Tehrani, G. Burwell, M. A. M. Azmi, A. Castaing, R. Rickman, J. Almarashi, P. Dunstan, A. M. Beigi, S. Doak, O. Guy
Published 2014 · Materials Science
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A generic electrochemical method of ?bioreceptor? antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (nM) concentrations of the cancer risk (oxidative stress) biomarker 8-hydroxydeoxyguanosine (8-OHdG). X-ray photoelectron spectroscopy, Raman spectroscopy are used to characterize the functionalized MLEG. Confocal fluorescence microscopy using fluorescent-labelled antibodies indicates that the anti-8-OHdG antibody selectively binds to the phenyl amine-functionalized MLEG?s channel. Current?voltage measurements on functionalized channels showed repeatable current responses from antibody?biomarker binding events. This technique is scalable, reliable, and capable of providing a rapid, quantitative, label-free assessment of biomarkers at nano-molar (<20 nM) concentrations in analyte solutions. The sensitivity of the sensor device was investigated using varying concentrations of 8-OHdG, with changes in the sensor?s channel resistance observed upon exposure to 8-OHdG. Detection of 8-OHdG concentrations as low as 0.1 ng ml?1 (0.35 nM) has been demonstrated. This is five times more sensitive than reported enzyme linked immunosorbent assay tests (0.5 ng ml?1).
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