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Novel Starch-based Scaffolds For Bone Tissue Engineering: Cytotoxicity, Cell Culture, And Protein Expression.

António J Salgado, Olga P. Coutinho, Rui Luís Reis
Published 2004 · Biology, Medicine
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Starch-based biomaterials and scaffolds have been proposed for several biomedical applications. In the present work new scaffolds based on a 50/50 (wt%) blend of corn starch/ethylene-vinyl alcohol (SEVA-C) were studied. These scaffolds were processed by a melt-based technology, which has been used before with other starch-based materials but never with SEVA-C. Scanning electron microscopy (SEM) observation showed that the developed porous structures were 60% porous with pore size between 200 and 900 microm and a reasonable degree of interconnectivity. Moreover, scaffolds presented a compressive modulus of 117.50 +/- 3.7 MPa and a compressive strength of 20.8 +/- 2.4 MPa. Cytotoxicity evaluation was performed according to ISO/EN 10993 part 5 guidelines, and revealed that the developed scaffolds were nontoxic and did not inhibit cell growth. Direct contact assays were also carried out by use of a cell line of human osteoblast-like cells (SaOS-2). Cells were seeded (3 x 10(5) per scaffold) and allowed to grow for 4 weeks at 37 degrees C, in a humidified atmosphere containing 5% CO(2). Total protein assay showed that the cells were able to grow for the 4 weeks of the experiment. These data were further confirmed by SEM. Moreover, a cell viability assay (MTS test) demonstrated that cells were perfectly viable after the 4 weeks of culture, showing the adequacy of the developed structure in supporting them. Finally, Western blot analysis revealed that osteopontin was being actively expressed by the cells, which, in association with collagen deposition observed by SEM, seems to indicate that bone extracellular matrix was being deposited. Consequently it is believed that starch-based scaffolds should be considered as an alternative for bone tissue-engineering applications in the near future.
This paper references
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J. Sodek (2000)
Three-dimensional printing and porous metallic surfaces: a new orthopedic application.
Mora Carolynne Melican (2001)
The design of scaffolds for use in tissue engineering. Part I. Traditional factors.
Shoufeng Yang (2001)
Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds.
R C Thomson (1999)
ISO document 10993 . Biological compatibility of medical devices . 5 . Test for cytotoxicity : In vitro methods
A. H. Cory (1992)
Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture.
Ann H. Cory (1991)
Injectable biodegradable biomaterials for orthopaedic tissue engineering
J. S. Temenoff (2000)
Biocompatibility testing of novel starch-based materials with potential application in orthopaedic surgery: a preliminary study.
Sandra Denise Camargo Mendes (2001)
Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds.
Lonnie D Shea (2000)
Enhancement of the in vivo osteogenic potential of marrow/hydroxyapatite composites by bovine bone morphogenetic protein.
Toshiaki Noshi (2000)
Osteogenic differentiation of marrow stromal stem cells in porous hydroxyapatite ceramics.
Hajime Ohgushi (1993)
Development of soluble glasses for biomedical use Part II: The biological response of human osteoblast cell lines to phosphate-based soluble glasses
Vehid Max Salih (2000)
New starch-based thermoplastic hydrogels for use as bone cements or drug-delivery carriers
C. Pereira (1998)
Tissue-engineered bone regeneration
Hervé Petite (2000)
Design of Macroporous Biodegradable Polymer Scaffolds for Cell Transplantation
Véronique Maquet (1997)
Bone tissue engineering using synthetic biodegradable polymer scaffolds
J. S. Temenoff (1999)
Culture of organized cell communities.
Vunjak-Novakovic (1998)
Underlying mechanisms of cellular adhesion in vitro during coloniza SALGADO ET AL. tion of synthetic surfaces by bone-derived cells
J. G. Steele (1999)
Rapid postadsorptive changes in fibrinogen adsorbed from plasma to segmented polyurethanes.
Thomas A. Horbett (1998)
Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds
António J Salgado (2002)
A new approach based on injection moulding to produce biodegradable starch-based polymeric scaffolds: morphology, mechanical and degradation behaviour.
Manuela E Gomes (2001)
Bone graft and bone graft substitutes: a review of current technology and applications.
Christopher J. Damien (1991)
Alternative tissue engineering scaffolds based on starch: processing methodologies, morphology, degradation and mechanical properties
Manuela E Gomes (2002)
Processing and characterization of new porous biodegradable starch-based delivery system for arthritis and rheumatism treatment
P. B. Malafaya (2001)
Scaffolds in tissue engineering bone and cartilage.
Dietmar Werner Hutmacher (2000)
New degradable load-bearing biomaterials composed of reinforced starch based blends
Rui Luís Reis (2000)
Tissue engineering.
Richard Langer (1993)
Using Nonconventional Processing to Develop Anisotropic and Biodegradable Composites of Starch-Based Thermoplastics Reinforced with Bone-Like Ceramics
Rui Luís Reis (1998)
Co-extrusion of biocompatible polymers for scaffolds with co-continuous morphology.
Newell Washburn (2002)
Inhibition of mitogen-activated protein kinase activity of human lymphocytes after oral administration of Oltipraz.
Burra V. Madhukar (2002)
Stem cell technology and bioceramics: from cell to gene engineering.
Hajime Ohgushi (1999)
Engineering bone regeneration with bioabsorbable scaffolds with novel microarchitecture.
K Whang (1999)
Injectable biodegradable materials for orthopedic tissue engineering
Johnna S. Temenoff (2000)
Segmental bone repair by tissue-engineered periosteal cell transplants with bioresorbable fleece and fibrin scaffolds in rabbits.
Carsten Perka (2000)
Replacement of an avulsed phalanx with tissue-engineered bone.
Charles A. Vacanti (2001)
Dexamethasone and retinoic acid differentially regulate growth and differentiation in an immortalised human clonal bone marrow stromal cell line with osteoblastic characteristics
Nc Ogston (2002)
Starch and starch based blends
R. L. Reis (2001)

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Iva Pashkuleva (2010)
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Francesca Gatto (2016)
Sustainable starch modified polyol based tough, biocompatible, hyperbranched polyurethane with a shape memory attribute
Rituparna Duarah (2016)
Natural origin scaffolds with in situ pore forming capability for bone tissue engineering applications.
Ana M. Martins (2008)
- Biomaterials for Scaffolds: Synthetic Polymers
Claudio Migliaresi (2014)
Comparison of additive effects on the PVA/starch cryogels: Synthesis, characterization, cytotoxicity, and genotoxicity studies
Seda Ceylan (2018)
Novel polymeric systems based on natural materials : development and biological performance
Sandra Milena Luna Osório (2007)
Biomaterials of natural origin in regenerative medicine
Vijay Kumar Nandagiri (2013)
Development and characterization of a PHB-HV-based 3D scaffold for a tissue engineering and cell-therapy combinatorial approach for spinal cord injury regeneration.
Silvina Ribeiro-Samy (2013)
Effect of starch on the mechanical and in vitro properties of collagen-hydroxyapatite sponges for applications in dentistry.
Ana Bertha Castro-Ceseña (2016)
Role of Polymeric Biomaterials as Wound Healing Agents
Priyanka Agrawal (2014)
Effect of starch type on miscibility in poly(ethylene oxide) (PEO)/starch blends and cytotoxicity assays
Antonio G.B. Pereira (2011)
New strategies for bone graft materials
Sacha Cavelier (2016)
Engineering articular cartilage using newly developed carrageenan basedhydrogels
Elena Geta Popa (2014)
Cell Adhesion and Proliferation onto Chitosan-based Membranes Treated by Plasma Surface Modification
Sandra M Luna (2011)
Biomimetic apatite layer formation on a novel citrate starch scaffold suitable for bone tissue engineering applications
Jhamak Nourmohammadi (2016)
Tissue reaction after subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial.
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