Online citations, reference lists, and bibliographies.
← Back to Search

The Role Of Endogenous Eicosanoids In Rabbit-intraocular Inflammation.

P. Kulkarni
Published 1991 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The role of endogenously released eicosanoids in intraocular inflammation was assessed in two rabbit models. The models were: (1) paracentesis in which only breakdown of blood-aqueous barrier (BAB) occurs and (2) uveitis induced by endotoxin in which the disruption of the BAB and polymorphonuclear leukocyte infiltration are the predominant events. Indomethacin (a specific cyclooxygenase inhibitor) applied topically inhibited both the disruption of the BAB and increased levels of aqueous humor 6-keto-Prostaglandin (PG)F1 alpha. However, indomethacin and flurbiprofen applied topically and BWA4C or BWA218C (both selective lipoxygenase inhibitors) given parenterally, did not inhibit BAB response in endotoxin-induced uveitis. The cyclooxygenase inhibitors attenuated PGE2 release into aqueous humor. The 5-lipoxygenase inhibitors reduced the PMN infiltration as well as LTB4 release into aqueous humor. However, myeloperoxidase activity (an index for PMN chemotaxis) in iris-ciliary body was not affected by these drugs. Furthermore, concentrations of LTB4 in aqueous humor after paracentesis and uveitis-induced by endotoxin were similar, although in the former model there was no leukocyte infiltration, but in the latter model this leukocyte response was predominant. The results of this study suggest that locally released autocoids may not initiate ocular inflammation and other mediators such as cytokines may be involved in the inflammatory responses of the rabbit eye. We tried to detect IL-1 activity in aqueous humor following endotoxin. However, we could not detect the presence of IL-1-like activity, possibly because endotoxin also releases PGs, which inhibit IL-1 bioassay.



This paper is referenced by
10.1016/S0039-6257(02)00306-5
Effects of prostaglandins on the aqueous humor outflow pathways.
R. Weinreb (2002)
10.1016/j.jcjo.2014.10.011
Posterior lens capsular neovascularization of young: management using endodiathermy assisted biopsy.
S. Gupta (2015)
10.1016/S0142-9612(02)00080-7
Flurbiprofen-loaded acrylate polymer nanosuspensions for ophthalmic application.
R. Pignatello (2002)
10.2147/OPTH.S1067
Topical ophthalmic NSAIDs: a discussion with focus on nepafenac ophthalmic suspension
Bruce I. Gaynes (2008)
10.1089/JOP.1994.10.335
Prevention and treatment of ocular inflammation with a new class of non-steroidal anti-inflammatory agents.
G. Chiou (1994)
10.1007/BF00184087
Explorative study of interleukin levels in the human cornea
J. Becker (2004)
10.1016/S0049-3848(03)00415-8
COX-2 lack of function in hypoxia-induced ocular surface inflammation.
M. Schwartzman (2003)
10.1076/ceyr.23.5.372.5439
The effect of partial vitrectomy on blood-ocular barrier function in the rabbit
W. Garner (2001)
10.1185/030079906X89775
Ocular permeation and inhibition of retinal inflammation: an examination of data and expert opinion on the clinical utility of nepafenac
R. Lindstrom (2006)
10.5772/58229
Regional Immune, Hormonal and Mediatory Mechanisms Responsible for Function of “Immune Privilege of an Eye” at Senile and Complicated Cataracts
A. Zilfyan (2014)
10.1111/j.2042-7158.1995.tb06727.x
Effect of Sodium Naproxen on Inflammatory Response Induced by Anterior Chamber Paracentesis in the Rabbit
C. Bucolo (1995)
10.1007/s000110050230
The effects of betamethasone derivatives on endotoxin-induced uveitis in guinea pigs
F. Tsuji (1997)
10.2460/AJVR.2002.63.750
Laser flaremetric evaluation of experimentally induced blood-aqueous barrier disruption in cats.
A. Rankin (2002)
10.2460/AJVR.2004.65.1325
Effects of intracameral injection of preservative-free lidocaine on the anterior segment of the eyes in dogs.
P. Gerding (2004)
10.1076/OCII.6.4.227.4030
Transient loss of prostaglandin synthetic capacity in rabbit iris-ciliary body following anterior chamber paracentesis.
G. Graff (1998)
10.1016/S0928-0987(02)00057-X
Eudragit RS100 nanosuspensions for the ophthalmic controlled delivery of ibuprofen.
R. Pignatello (2002)
OCULAR INFLAMMATION AND NSAIDS: AN OVERVIEW WITH SELECTIVE AND NON-SELECTIVE COX INHIBITORS
R. Rao (2016)
10.1007/s11306-020-1650-y
Comparative lipidomic analysis of inflammatory mediators in the aqueous humor and tear fluid of humans and rabbits
D. Chistyakov (2020)
10.1016/J.EXER.2004.08.030
Anti-inflammatory effects of topical ocular MAXIDEX administration to rabbits following vitrectomy or lensectomy.
S. Scheib (2004)
10.1089/JOP.1994.10.329
Steroidal and nonsteroidal drugs in endotoxin-induced uveitis.
P. Kulkarni (1994)
10.1111/bph.12873
Treatment with melatonin after onset of experimental uveitis attenuates ocular inflammation
P. Sande (2014)
10.1080/09273940590928643
Anti-Inflammatory Effects of Specific Cyclooxygenase 2,5-Lipoxygenase, and Inducible Nitric Oxide Synthase Inhibitors on Experimental Autoimmune Anterior Uveitis (EAAU)
N. Bora (2005)
Reduction of collagen type I in the ciliary muscle of inflamed monkey eyes.
T. Sagara (1999)
10.1007/978-3-319-78364-2_2
Standard Operating Procedures for Common Laboratory Animal Ocular Procedures
B. Gilger (2018)
Semantic Scholar Logo Some data provided by SemanticScholar