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Anisotropic Shape-Memory Alginate Scaffolds Functionalized With Either Type I Or Type II Collagen For Cartilage Tissue Engineering.

Henrique V. Almeida, Binulal Nelson Sathy, Ivan Dudurych, Conor Timothy Buckley, Fergal Obrien, Daniel J. Kelly
Published 2017 · Biology, Medicine
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Regenerating articular cartilage and fibrocartilaginous tissue such as the meniscus is still a challenge in orthopedic medicine. While a range of different scaffolds have been developed for joint repair, none have facilitated the development of a tissue that mimics the complexity of soft tissues such as articular cartilage. Furthermore, many of these scaffolds are not designed to function in mechanically challenging joint environments. The overall goal of this study was to develop a porous, biomimetic, shape-memory alginate scaffold for directing cartilage regeneration. To this end, a scaffold was designed with architectural cues to guide cellular and neo-tissue alignment, which was additionally functionalized with a range of extracellular matrix cues to direct stem cell differentiation toward the chondrogenic lineage. Shape-memory properties were introduced by covalent cross-linking alginate using carbodiimide chemistry, while the architecture of the scaffold was modified using a directional freezing technique. Introducing such an aligned pore structure was found to improve the mechanical properties of the scaffold, and promoted higher levels of sulfated glycosaminoglycans (sGAG) and collagen deposition compared to an isotropic (nonaligned) pore geometry when seeded with adult human stem cells. Functionalization with collagen improved stem cell recruitment into the scaffold and facilitated more homogenous cartilage tissue deposition throughout the construct. Incorporating type II collagen into the scaffolds led to greater cell proliferation, higher sGAG and collagen accumulation, and the development of a stiffer tissue compared to scaffolds functionalized with type I collagen. The results of this study demonstrate how both scaffold architecture and composition can be tailored in a shape-memory alginate scaffold to direct stem cell differentiation and support the development of complex cartilaginous tissues.
This paper references
Determination of hydroxyproline in tissues and the evaluation of the collagen content of the tissues
N. Yu. Ignat’eva (2007)
Multilineage cells from human adipose tissue: implications for cell-based therapies.
Patricia A. Zuk (2001)
Micrometer scale guidance of mesenchymal stem cells to form structurally oriented cartilage extracellular matrix.
Chih-Ling Chou (2013)
Advanced cell therapies for articular cartilage regeneration.
Catarina Madeira (2015)
Aligned two- and three-dimensional structures by directional freezing of polymers and nanoparticles
Haifei Zhang (2005)
Extracellular matrix scaffolds for cartilage and bone regeneration.
Kim E M Benders (2013)
The bioactivity of cartilage extracellular matrix in articular cartilage regeneration.
Amanda J. Sutherland (2015)
Marrow stimulation techniques.
Matthias R Steinwachs (2008)
Shape-memory porous alginate scaffolds for regeneration of the annulus fibrosus: effect of TGF-β3 supplementation and oxygen culture conditions.
Olivier Guillaume (2014)
3D bioprinting of tissues and organs
Sean V Murphy (2014)
Autologous Osteochondral Mosaicplasty for the Treatment of Full-Thickness Defects of Weight-Bearing Joints: Ten Years of Experimental and Clinical Experience
László Rudolf Hangody (2003)
Alginate–Hydroxyapatite Bone Scaffolds with Isotropic or Anisotropic Pore Structure: Material Properties and Biological Behavior
Davide Porrelli (2015)
Cell attachment, collagen binding, and receptor analysis on bovine articular chondrocytes.
D L Reid (2000)
Coupling Freshly Isolated CD44(+) Infrapatellar Fat Pad-Derived Stromal Cells with a TGF-β3 Eluting Cartilage ECM-Derived Scaffold as a Single-Stage Strategy for Promoting Chondrogenesis.
Henrique V. Almeida (2015)
Enhancing cell migration in shape-memory alginate–collagen composite scaffolds: In vitro and ex vivo assessment for intervertebral disc repair
Olivier Guillaume (2015)
Canine chondrocytes seeded in type I and type II collagen implants investigated in vitro.
Stefan Nehrer (1997)
Design and fabrication of a biodegradable, covalently crosslinked shape-memory alginate scaffold for cell and growth factor delivery.
Lin Lin Wang (2012)
Laminar silk scaffolds for aligned tissue fabrication.
Biman B. Mandal (2013)
Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis.
Darko Bosnakovski (2006)
PCL–gelatin composite nanofibers electrospun using diluted acetic acid–ethyl acetate solvent system for stem cell-based bone tissue engineering
N. S. Binulal (2014)
Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
J.A.M. Steele (2014)
Comparative phenotypic analysis of articular chondrocytes cultured within type I or type II collagen scaffolds.
A. M. Freyria (2009)
Incorporation of TGF-beta 3 within collagen-hyaluronic acid scaffolds improves their chondrogenic potential.
Amos Matsiko (2015)
Infrapatellar fat pad-derived stem cells maintain their chondrogenic capacity in disease and can be used to engineer cartilaginous grafts of clinically relevant dimensions.
Yurong Liu (2014)
Towards the design of 3D multiscale instructive tissue engineering constructs: Current approaches and trends.
Sara M. Oliveira (2015)
Mechanical characterization of matrix-induced autologous chondrocyte implantation (MACI®) grafts in an equine model at 53 weeks.
Darvin J Griffin (2015)
Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering.
Shuaijun Jia (2012)
Fibronectin fragments and blocking antibodies to alpha2beta1 and alpha5beta1 integrins stimulate mitogen-activated protein kinase signaling and increase collagenase 3 (matrix metalloproteinase 13) production by human articular chondrocytes.
Christopher B Forsyth (2002)
Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs.
Conor Timothy Buckley (2012)
A biomimetic multi-layered collagen-based scaffold for osteochondral repair.
Tanya J Levingstone (2014)
Effect of collagen II coating on mesenchymal stem cell adhesion on chitosan and on reacetylated chitosan fibrous scaffolds
Guillaume R. Ragetly (2010)
Engineering cartilage or endochondral bone: a comparison of different naturally derived hydrogels.
Eamon J. Sheehy (2015)
Effect of collagen type I or type II on chondrogenesis by cultured human articular chondrocytes.
Marijn Rutgers (2013)
The role of the superficial region in determining the dynamic properties of articular cartilage.
A R Gannon (2012)
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis.
Guillaume R. Ragetly (2010)
The effects of dynamic compression on the development of cartilage grafts engineered using bone marrow and infrapatellar fat pad derived stem cells.
Lu Luo (2015)
Cartilaginous extracellular matrix-modified chitosan hydrogels for cartilage tissue engineering.
Bogyu Choi (2014)
Engineering growing tissues
Eben Alsberg (2002)
The isolation of two types of collagen from embryonic bovine epiphyseal cartilage
Jerome M. Seyer (2006)
Matrix collagen type and pore size influence behaviour of seeded canine chondrocytes.
Stefan Nehrer (1997)
Cyclic hydrostatic pressure promotes a stable cartilage phenotype and enhances the functional development of cartilaginous grafts engineered using multipotent stromal cells isolated from bone marrow and infrapatellar fat pad.
Simon F. Carroll (2014)
Fibronectin fragments and blocking antibodies to α2β1 and α5β1 integrins stimulate mitogen-activated protein kinase signaling and increase collagenase 3 (matrix metalloproteinase 13) production by human articular chondrocytes
Christopher B Forsyth (2002)
Combined Effect of a Microporous Layer and Type I Collagen Coating on a Biphasic Calcium Phosphate Scaffold for Bone Tissue Engineering
Mun-Hwan Lee (2015)
Mechano-electrochemical properties of articular cartilage: their inhomogeneities and anisotropies.
Van C. Mow (2002)
Growth factor regulation of chondrocyte integrins. Differential effects of insulin-like growth factor 1 and transforming growth factor beta on alpha 1 beta 1 integrin expression and chondrocyte adhesion to type VI collagen.
Richard F. Loeser (1997)
Biochemical and physiochemical characterization of pepsin-solubilized type-II collagen from bovine articular cartilage.
Daniel Herbage (1977)
Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications.
Patrícia B. Malafaya (2007)
Autologous Chondrocyte Implantation With Collagen Membrane. Sports medicine and arthroscopy
SD Gillogly (2015)
Scaffolds in tissue engineering bone and cartilage.
Dietmar Werner Hutmacher (2000)
Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold.
Masaya Yamamoto (2010)
Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair.
Abigail M. Wojtowicz (2010)
Electrospinning: applications in drug delivery and tissue engineering.
T J Sill (2008)
Methodologies for processing biodegradable and natural origin scaffolds for bone and cartilage tissue-engineering applications.
Manuela E Gomes (2004)
The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering.
Ciara M. Murphy (2010)
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
D L Reid (2000)
Chondrocyte Aggregation in Suspension Culture Is GFOGER-GPP- and β1 Integrin-dependent*
Anne Gigout (2008)
Meniscus tissue engineering on the nanoscale: from basic principles to clinical application.
Brendon M Baker (2009)
Complexity in biomaterials for tissue engineering.
Elsie S. Place (2009)
Accordion-Like Honeycombs for Tissue Engineering of Cardiac Anisotropy
George C. Engelmayr (2008)
The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering.
Steven R. Caliari (2011)
Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells.
Henrique V. Almeida (2014)
Functional properties of cartilaginous tissues engineered from infrapatellar fat pad-derived mesenchymal stem cells.
Conor Timothy Buckley (2010)
Integrins and cell signaling in chondrocytes.
Richard F. Loeser (2002)
Growth factor regulation of chondrocyte integrins. Differential effects of insulin-like growth factor 1 and transforming growth factor β on α1β1 integrin expression and chondrocyte adhesion to type VI collagen
Richard F. Loeser (1997)
Autologous Chondrocyte Implantation With Collagen Membrane.
Scott D. Gillogly (2015)
Crosslinked type II collagen matrices: preparation, characterization, and potential for cartilage engineering.
J S Pieper (2002)
Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels
Magnus Ø. Olderøy (2014)
Signaling "cross-talk" between TGF-beta1 and ECM signals in chondrocytic cells.
Michaela M Schneiderbauer (2004)
Autologous Chondrocyte Implantation With Collagen Membrane. Sports medicine and arthroscopy review
S D Gillogly (2015)
Localization of beta 1-integrins in human cartilage and their role in chondrocyte adhesion to collagen and fibronectin.
J. Dürr (1993)
Osteoarthritis of the knee.
David L. Scott (2007)
A Calcium-Cross-Linked Hydrogel Based on Alginate-Modified Atelocollagen Functions as a Scaffold Material
Wataru Kamimura (2012)
Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering.
N. William Garrigues (2014)
Aligned biodegradable nanofibrous structure: a potential scaffold for blood vessel engineering.
Chengyu Xu (2004)
Pore orientation mediated control of mechanical behavior of scaffolds and its application in cartilage-mimetic scaffold design.
A. Arora (2015)
Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Edward John Miller (1972)

This paper is referenced by
Engineering the Interface: Hyaluronic Acid Hydrogels that Mediate MSC Chondrogenesis for Cartilage Tissue Engineering
Mi Young Kwon (2019)
Functionally Graded Biomaterials for Use as Model Systems and Replacement Tissues
Jeremy Lowen (2020)
Scaffold channel size influences stem cell differentiation pathway in 3-D printed silica hybrid scaffolds for cartilage regeneration.
Siwei Li (2020)
Glyoxal cross-linking of solubilized extracellular matrix to produce highly porous, elastic, and chondro-permissive scaffolds for orthopedic tissue engineering.
David C Browe (2019)
Progress in Articular Cartilage Tissue Engineering: A Review on Therapeutic Cells and Macromolecular Scaffolds.
Zhongyi Zhao (2019)
A water-responsive shape memory ionomer with permanent shape reconfiguration ability
Yongkang Bai (2018)
Mesenchymal Stem Cells in Oriented PLGA/ACECM Composite Scaffolds Enhance Structure-Specific Regeneration of Hyaline Cartilage in a Rabbit Model
Weimin Guo (2018)
Engineering biologically extensible hydrogels using photolithographic printing.
S. M. Mehta (2018)
Tissue-specific extracellular matrix scaffolds for the regeneration of spatially complex musculoskeletal tissues.
Gráinne M. Cunniffe (2019)
Effects of solid acellular type-I/III collagen biomaterials on in vitro and in vivo chondrogenesis of mesenchymal stem cells
Liang Gao (2017)
Porous Scaffolds Derived from Devitalized Tissue Engineered Cartilaginous Matrix Support Chondrogenesis of Adult Stem Cells
Henrique V. Almeida (2017)
Construction of Tissue-Engineered Scaffolds in Vitro by Using Medical Gelatin Sponge and Sodium Alginate Hydrogel
Zhongyi Zhao (2020)
Bio-instructive materials for musculoskeletal regeneration.
Tomas Gonzalez-Fernandez (2019)
Enzymatically triggered shape memory polymers.
Shelby L Buffington (2019)
Urtica extracts induce periosteal cell proliferation and differentiation: tissue‐engineered bone construction and ultrastructural changes
Bing Xu (2018)
Tissue Engineering for the Temporomandibular Joint.
Timothy M. Acri (2019)
Recent advances in hydrogels for cartilage tissue engineering.
Sebastián L Vega (2017)
Meniscus ECM-functionalised hydrogels containing infrapatellar fat pad-derived stem cells for bioprinting of regionally defined meniscal tissue.
S Romanazzo (2018)
An in vitro and in vivo comparison of cartilage growth in chondrocyte-laden matrix metalloproteinase-sensitive poly(ethylene glycol) hydrogels with localized transforming growth factor β3.
Margaret C Schneider (2019)
Alginate Based Scaffolds for Cartilage Tissue Engineering: A Review
Maryam Farokhi (2020)
Conductive nanostructured Si biomaterials enhance osteogeneration through electrical stimulation.
Yan Huang (2019)
Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques
Hamed Amani (2019)
Unidirectional BMP2-loaded collagen scaffolds induce chondrogenic differentiation.
Michiel W Pot (2017)
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