Online citations, reference lists, and bibliographies.
← Back to Search

Phospholipase D Is Involved In Myogenic Differentiation Through Remodeling Of Actin Cytoskeleton.

H. Komati, F. Naro, S. Mebarek, V. De Arcangelis, S. Adamo, M. Lagarde, A. Prigent, G. Némoz
Published 2005 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
We investigated the role of phospholipase D (PLD) and its product phosphatidic acid (PA) in myogenic differentiation of cultured L6 rat skeletal myoblasts. Arginine-vasopressin (AVP), a differentiation inducer, rapidly activated PLD in a Rho-dependent way, as shown by almost total suppression of activation by C3 exotoxin pretreatment. Addition of 1-butanol, which selectively inhibits PA production by PLD, markedly decreased AVP-induced myogenesis. Conversely, myogenesis was potentiated by PLD1b isoform overexpression but not by PLD2 overexpression, establishing that PLD1 is involved in this process. The expression of the PLD isoforms was differentially regulated during differentiation. AVP stimulation of myoblasts induced the rapid formation of stress fiber-like actin structures (SFLSs). 1-Butanol selectively inhibited this response, whereas PLD1b overexpression induced SFLS formation, showing that it was PLD dependent. Endogenous PLD1 was located at the level of SFLSs, and by means of an intracellularly expressed fluorescent probe, PA was shown to be accumulated along these structures in response to AVP. In addition, AVP induced a PLD-dependent neosynthesis of phosphatidylinositol 4,5-bisphosphate (PIP2), which also was accumulated along actin fibers. These data support the hypothesis that PLD participates in myogenesis through PA- and PIP2-dependent actin fiber formation.
This paper references
Cloning, differential regulation and tissue distribution of alternatively spliced isoforms of ADP-ribosylation-factor-dependent phospholipase D from rat liver.
K. Katayama (1998)
Signal-Regulated Activation of Serum Response Factor Is Mediated by Changes in Actin Dynamics
A. Sotiropoulos (1999)
Mol. Biol. Cell
Mechanism of ADP Ribosylation Factor-stimulated Phosphatidylinositol 4,5-Bisphosphate Synthesis in HL60 Cells*
A. Skippen (2002)
Controlling cytoskeleton structure by phosphoinositide-protein interactions: phosphoinositide binding protein domains and effects of lipid packing.
P. Janmey (1999)
Interaction of the Type Iα PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4,5‐bisphosphate in the regulation of PLD2 activity
N. Divecha (2000)
Stress fibres take shape
A. Ridley (1999)
Interactions between IFs, Microtubules, and Myofibrils in Fibrogenic and Myogenic Cells a
H. Holtzer (1985)
Stimulation of actin stress fibre formation mediated by activation of phospholipase D
M. Cross (1996)
The Rho family GTPases RhoA, Racl , and CDC42Hsregulate transcriptional activation by SRF
C. Hill (1995)
RhoA effector mutants reveal distinct effector pathways for cytoskeletal reorganization, SRF activation and transformation
E. Sahai (1998)
Human Phospholipase D1 Can Be Tyrosine-phosphorylated in HL-60 Granulocytes*
J. Marcil (1997)
Molecular Biology of the Cell
J. Davies (1983)
Role of phospholipase C and D signalling pathways in vasopressin‐dependent myogenic differentiation
F. Naro (1997)
Transduction of arginine vasopressin signal in skeletal myogenic cells.
A. Teti (1993)
Activation of protein kinase C in rat basophilic leukemia cells stimulates increased production of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: correlation with actin polymerization.
J. Apgar (1995)
Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization
W. C. Colley (1997)
Insulin stimulates phospholipase D-dependent phosphatidylcholine hydrolysis, Rho translocation, de novo phospholipid synthesis, and diacylglycerol/protein kinase C signaling in L6 myotubes.
M. Standaert (1996)
Modulation of Rho GTPase Signaling Regulates a Switch between Adipogenesis and Myogenesis
R. Sordella (2003)
Molecular mechanisms regulating myogenic determination and differentiation.
R. L. Perry (2000)
The relationship between stress fiber-like structures and nascent myofibrils in cultured cardiac myocytes
A. Dlugosz (1984)
Actin Directly Interacts with Phospholipase D, Inhibiting Its Activity*
Sukmook Lee (2001)
Vesicle-mediated phosphatidylcholine reapposition to the plasma membrane following hormone-induced phospholipase D activation.
D. Coletti (2000)
Phosphatidylinositol 4-Phosphate 5-Kinase α Is a Downstream Effector of the Small G Protein ARF6 in Membrane Ruffle Formation
A. Honda (1999)
Raf-1 Kinase Possesses Distinct Binding Domains for Phosphatidylserine and Phosphatidic Acid
S. Ghosh (1996)
Vasopressin and insulin-like growth factors synergistically induce myogenesis in serum-free medium.
S. Minotti (1998)
Associations of B- and C-Raf with Cholesterol, Phosphatidylserine, and Lipid Second Messengers
M. Hekman (2002)
Lysophosphatidic acid activation of phosphatidylcholine-hydrolysing phospholipase D and actin polymerization by a pertussis toxin-sensitive mechanism.
Kwon Soo Ha (1994)
Type Iα phosphatidylinositol 4‐phosphate 5‐kinase is a putative target for increased intracellular phosphatidic acid
D. Jones (2000)
AVP induces myogenesis through the transcriptional activation of the myocyte enhancer factor 2.
B. M. Scicchitano (2002)
Stimulation of protein synthesis and phospholipase D activity by vasopressin and phorbol ester in L6 myoblasts.
M. Thompson (1994)
Segregation of phosphatidic acid-rich domains in reconstituted acetylcholine receptor membranes.
J. A. Poveda (2002)
The Mechanism of Docosahexaenoic Acid-induced Phospholipase D Activation in Human Lymphocytes Involves Exclusion of the Enzyme from Lipid Rafts*
O. Diaz (2002)
Sustained phospholipase D activation is associated with keratinocyte differentiation.
E. M. Jung (1999)
Titin aggregates associated with intermediate filaments align along stress fiber-like structures during human skeletal muscle cell differentiation.
P. F. van der Ven (1993)
J. Cell. Biol
J. Biol. Chem
RhoA Signaling via Serum Response Factor Plays an Obligatory Role in Myogenic Differentiation*
L. Wei (1998)
Rho GTPases*
D. Mackay (1998)
The Rho Family G Proteins Play a Critical Role in Muscle Differentiation
H. Takano (1998)
Phosphatidylinositol 4,5-Bisphosphate Induces Actin Stress-Fiber Formation and Inhibits Membrane Ruffling in Cv1 Cells
M. Yamamoto (2001)
Phospholipase D Activity Is Required for Actin Stress Fiber Formation in Fibroblasts
Y. Kam (2001)
Actin Dynamics Control SRF Activity by Regulation of Its Coactivator MAL
F. Miralles (2003)
Differential involvement of Galpha12 and Galpha13 in receptor-mediated stress fiber formation.
A. Gohla (1999)
Differential Involvement of Gα12 and Gα13 in Receptor-mediated Stress Fiber Formation*
A. Gohla (1999)
Rho as a mediator of G protein-coupled receptor signaling.
T. M. Seasholtz (1999)
Regulation of Phospholipase D Activity by Actin
D. Kusner (2002)
Possible role of phospholipase D in cellular differentiation and apoptosis.
S. Nakashima (1999)
Regulation of phospholipase D
J. Exton (1999)
The cAMP-specific Phosphodiesterase PDE4D3 Is Regulated by Phosphatidic Acid Binding
M. Grange (2000)
Phosphatidylinositol Phosphate Kinases, a Multifaceted Family of Signaling Enzymes*
R. Anderson (1999)
Arginine-vasopressin induces differentiation of skeletal myogenic cells and up-regulation of myogenin and Myf-5.
C. Nervi (1995)
The Recruitment of Raf-1 to Membranes Is Mediated by Direct Interaction with Phosphatidic Acid and Is Independent of Association with Ras*
M. Rizzo (2000)
Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells.
N. O'luanaigh (2002)
Type I phosphatidylinositol 4-phosphate 5-kinase isoforms are specifically stimulated by phosphatidic acid.
G. H. Jenkins (1994)
Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells.
D. Powner (2002)
Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts
Kwon Soo Ha (1993)
Dual requirement for rho and protein kinase C in direct activation of phospholipase D1 through G protein-coupled receptor signaling.
G. Du (2000)
RhoA GTPase and serum response factor control selectively the expression of MyoD without affecting Myf5 in mouse myoblasts.
G. Carnac (1998)
Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins.
M. Hodgkin (1999)
Involvement of type 4 cAMP-phosphodiesterase in the myogenic differentiation of L6 cells.
F. Naro (1999)
Phospholipase D and Its Product, Phosphatidic Acid, Mediate Agonist-dependent Raf-1 Translocation to the Plasma Membrane and the Activation of the Mitogen-activated Protein Kinase Pathway*
M. Rizzo (1999)
A rapid method of total lipid extraction and purification.
E. Bligh (1959)
The simultaneous production of phosphatidic acid and diacylglycerol is essential for the translocation of protein kinase Cepsilon to the plasma membrane in RBL-2H3 cells.
Maria Jose Lopez-Andreo (2003)
Molecular mechanisms regulating myogenic determination and differentiation.
Perry Rl (2000)
Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton.
N. Tapon (1997)
Characterization of Two Alternately Spliced Forms of Phospholipase D1
S. Hammond (1997)
Association of Phospholipase D Activity with the Detergent-insoluble Cytoskeleton of U937 Promonocytic Leukocytes*
S. Iyer (1999)

This paper is referenced by
A transcriptomics study of differentiated C2C12 myoblasts identified novel functional responses to 17β‐estradiol
Jingjing Ding (2018)
Phospholipase D in the human ocular surface and in pterygium.
L. Tong (2008)
The effect of granulocyte-macrophage-stimulating factor on the gene expression in human trophoblast of early pregnancy
H. Lee (2010)
Cortactin, an actin binding protein, regulates GLUT4 translocation via actin filament remodeling
H. Nazari (2011)
Phospholipase D in human blood platelets
M. Vorland (2008)
Gliotoxin promotes Aspergillus fumigatus internalization into type II human pneumocyte A549 cells by inducing host phospholipase D activation.
Xiaodong Jia (2014)
Internalization of Aspergillus fumigatus into pulmonary epithelial cells: joint action of host and pathogen
Xuelin Han (2017)
Disruption of the Phospholipase D Gene Attenuates the Virulence of Aspergillus fumigatus
Xian-ping Li (2011)
The microfilament cytoskeleton plays a vital role in salt and osmotic stress tolerance in Arabidopsis.
C. Wang (2010)
Effects of MLS laser on myoblast cell line C 2 C 12
L. Vignali ()
Phospholipase D1 facilitates second-phase myoblast fusion and skeletal muscle regeneration
S. Teng (2015)
Silver sub-nanoclusters electrocatalyze ethanol oxidation and provide protection against ethanol toxicity in cultured mammalian cells.
J. Selva (2010)
Modulation of Caspase Activity Regulates Skeletal Muscle Regeneration and Function in Response to Vasopressin and Tumor Necrosis Factor
V. Moresi (2009)
A Mutation in Epstein–Barr Virus LMP2A Reveals a Role for Phospholipase D in B‐Cell Antigen Receptor Trafficking
Michelle L D Snyder (2006)
Signaling functions of phosphatidic acid.
X. Wang (2006)
LACTB promotes metastasis of nasopharyngeal carcinoma via activation of ERBB3/EGFR-ERK signaling resulting in unfavorable patient survival.
Li-Xia Peng (2020)
Myogenic differentiation depends on the interplay of Grb2 and N-WASP.
Payal Mitra (2017)
Plant phospholipases D and C and their diverse functions in stress responses.
Yueyun Hong (2016)
Sphingosine 1-phosphate induces myoblast differentiation through Cx43 protein expression: a role for a gap junction-dependent and -independent function.
R. Squecco (2006)
ADP-Ribosylation Factor 6 Regulates Mammalian Myoblast Fusion through Phospholipase D1 and Phosphatidylinositol 4,5-Bisphosphate Signaling Pathways
Anne-Sophie Bach (2010)
Limited Knowledge and Use of HIV Post- and Pre-Exposure Prophylaxis Among Gay and Bisexual Men
L. Tong (2008)
PLD regulates myoblast differentiation through the mTOR-IGF2 pathway
Mee-Sup Yoon (2008)
Effects of phospholipase D during cultured osteoblast mineralization and bone formation
D. Abdallah (2019)
Lysophosphatidic acid cooperates with 1alpha,25(OH)2D3 in stimulating human MG63 osteoblast maturation.
J. Gidley (2006)
An effector domain mutant of Arf6 implicates phospholipase D in endosomal membrane recycling.
Olivera A Jovanovic (2006)
Static magnetic fields enhance skeletal muscle differentiation in vitro by improving myoblast alignment
D. Coletti (2007)
Direct stimulation of receptor‐controlled phospholipase D1 by phospho‐cofilin
L. Han (2007)
Phosphatidic Acid Produced by RalA-activated PLD2 Stimulates Caveolae-mediated Endocytosis and Trafficking in Endothelial Cells*
Ying Jiang (2016)
Cytoskeleton/stretch‐activated ion channel interaction regulates myogenic differentiation of skeletal myoblasts
L. Formigli (2007)
The role of a phospholipase (PLD) in virulence of Purpureocillium lilacinum (Paecilomyces lilacinum).
F. Yang (2015)
β-1,3-Glucan-Induced Host Phospholipase D Activation Is Involved in Aspergillus fumigatus Internalization into Type II Human Pneumocyte A549 Cells
Xuelin Han (2011)
Phospholipase D regulates the size of skeletal muscle cells through the activation of mTOR signaling
Rami Jaafar (2012)
See more
Semantic Scholar Logo Some data provided by SemanticScholar