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Prevention Of UVB-induced Immunosuppression In Mice By The Green Tea Polyphenol (-)-epigallocatechin-3-gallate May Be Associated With Alterations In IL-10 And IL-12 Production.
S. Katiyar, A. Challa, T. Mccormick, K. Cooper, H. Mukhtar
Published 1999 · Medicine, Biology
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UV exposure of the skin, particularly UVB (290-320 nm), causes adverse biological effects, including alterations in cutaneous immune cells, photoaging and photocarcinogenesis. Several studies have shown that polyphenolic compounds isolated from green tea afford protection against UVB-induced inflammatory responses and photocarcinogenesis in murine models. In this study we show that topical application of (-)-epigallocatechin-3-gallate (EGCG) (3 mg/mouse), a major polyphenolic component of green tea, before a single low dose UVB exposure (72 mJ/cm(2)) to C3H/HeN mice prevented UVB-induced inhibition of the contact hypersensitivity response and tolerance induction to the contact sensitizer 2, 4-dinitrofluorobenzene. Topical application of EGCG before UVB exposure reduced the number of CD11b+ monocytes/macrophages and neutrophils infiltrating into skin inflammatory lesions, which are considered to be responsible for creating the UV-induced immunosuppressive state. In addition, application of EGCG before UVB exposure decreased UVB-induced production of the immunomodulatory cytokine interleukin (IL)-10 in skin as well as in draining lymph nodes (DLN), whereas production of IL-12, which is considered to be a mediator and adjuvant for induction of contact sensitivity, was found to be markedly increased in DLN when compared with UVB alone-exposed mice. Taken together, our data demonstrate that EGCG protects against UVB-induced immunosuppression and tolerance induction by: (i) blocking UVB-induced infiltration of CD11b+ cells into the skin; (ii) reducing IL-10 production in skin as well as in DLN; (iii) markedly increasing IL-12 production in DLN. Protection against UVB-induced immunosuppression by EGCG may be associated with protection against UVB-induced photocarcinogenesis.
This paper references
Biological properties of interleukin 10.
M. Howard (1992)
Inhibitory effects of black tea, green tea, decaffeinated black tea, and decaffeinated green tea on ultraviolet B light-induced skin carcinogenesis in 7,12-dimethylbenz[a]anthracene-initiated SKH-1 mice.
Z. Wang (1994)
Ultraviolet light depletes surface markers of Langerhans cells.
W. Aberer (1981)
Epidermal Langerhans cell density determines whether contact hypersensitivity or unresponsiveness follows skin painting with DNFB.
G. Toews (1980)
M. Kripke (1990)
The role of IL‐4, IL‐10, and TNF‐α in the immune suppression induced by ultraviolet radiation
J. M. Rivas (1994)
Cells with UV-specific DNA damage are present in murine lymph nodes after in vivo UV irradiation.
Y. Sontag (1995)
Protection of UV‐induced Suppression of Skin Contact Hypersensitivity: A Common Feature of Flavonoids after Oral Administration?
P. A. Steerenberg (1998)
Prevention of photocarcinogenesis by topical administration of pure epigallocatechin gallate isolated from green tea.
H. Gensler (1996)
Sunscreens prevent ultraviolet photocarcinogenesis.
L. Kligman (1980)
IL-10 inhibits cytokine production by activated macrophages.
D. Fiorentino (1991)
Wide distribution of [3H](-)-epigallocatechin gallate, a cancer preventive tea polyphenol, in mouse tissue.
M. Suganuma (1998)
Tea antioxidants in cancer chemoprevention
S. Katiyar (1997)
Active induction of unresponsiveness (tolerance) to DNFB by in vivo ultraviolet-exposed epidermal cells is dependent upon infiltrating class II MHC+ CD11bbright monocytic/macrophagic cells.
C. Hammerberg (1994)
Reversal of immunosuppression inducible through ultraviolet-exposed skin by in vivo anti-CD11b treatment.
C. Hammerberg (1996)
Polyphenolic Antioxidant (‐)‐Epigallocatechin‐3‐Gallate from Green Tea Reduces UVB‐lnduced Inflammatory Responses and Infiltration of Leukocytes in Human Skin
S. Katiyar (1999)
Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B.
M. Litton (1994)
Neutrophils, differentiated macrophages, and monocyte/macrophage antigen presenting cells infiltrate murine epidermis after UV injury.
K. Cooper (1993)
Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro
G. Schuler (1985)
Analysis of the mechanism of unresponsiveness produced by haptens painted on skin exposed to low dose ultraviolet radiation
C. Elmets (1983)
Cultured epidermal Langerhans cells activate effector T cells for contact sensitivity.
C. Hauser (1990)
Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression
R. de Waal Malefyt (1991)
Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells
R. Manetti (1993)
Systemic suppression of delayed-type hypersensitivity by supernatants from UV-irradiated keratinocytes. An essential role for keratinocyte-derived IL-10.
J. M. Rivas (1992)
The ultraviolet C energy emitted from FS lamps contributes significantly to the induction of human erythema and murine ear edema.
D. Learn (1993)
Inhibition of the induction of contact hypersensitivity by a UV-mediated epidermal cytokine.
T. Schwarz (1986)
The effect of ultraviolet B irradiation and urocanic acid isomers on dendritic cell migration.
A. Moodycliffe (1992)
PROTECTION AGAINST ULTRAVIOLET‐B RADIATION‐INDUCED LOCAL and SYSTEMIC SUPPRESSION OF CONTACT HYPERSENSITIVITY and EDEMA RESPONSES IN C3H/HeN MICE BY GREEN TEA POLYPHENOLS
S. Katiyar (1995)
Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes
M. Kobayashi (1989)
Purification to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells.
A. Stern (1990)
Induction of hapten-specific tolerance by interleukin 10 in vivo
A. Enk (1994)
Hapten-specific tolerance induced by acute, low-dose ultraviolet B radiation of skin is mediated via interleukin-10.
H. Niizeki (1997)
Mechanism of systemic immune suppression by UV irradiation in vivo. II. The UV effects on number and morphology of epidermal Langerhans cells and the UV-induced suppression of contact hypersensitivity have different wavelength dependencies.
F. Noonan (1984)
Photobiologic considerations in photoradiation therapy.
J. A. Parrish (1983)
Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages.
C. Hsieh (1993)
IL-12 as mediator and adjuvant for the induction of contact sensitivity in vivo.
G. Müller (1995)
Protective effects of silymarin against photocarcinogenesis in a mouse skin model.
S. Katiyar (1997)
Interleukin-12 prevents ultraviolet B-induced local immunosuppression and overcomes UVB-induced tolerance.
A. Schwarz (1996)
Sunburn and p53 in the onset of skin cancer
A. Ziegler (1994)
IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells.
D. Fiorentino (1991)
The interleukin‐12 subunit p40 specifically inhibits effects of the interleukin‐12 heterodimer
F. Mattner (1993)
UVB irradiation decreases the magnitude of the Th1 response to hapten but does not increase the Th2 response
S. Saijo (1996)
In vivo effects of interleukin-10 on contact hypersensitivity and delayed-type hypersensitivity reactions.
A. Schwarz (1994)
IL-12: initiation cytokine for cell-mediated immunity.
P. Scott (1993)
Susceptibility to effects of UVB radiation on induction of contact hypersensitivity as a risk factor for skin cancer in humans.
T. Yoshikawa (1990)
Inhibition of Langerhans cell antigen-presenting function by IL-10. A role for IL-10 in induction of tolerance.
A. Enk (1993)
Neutralization of IL-12 in vivo prevents induction of contact hypersensitivity and induces hapten-specific tolerance.
H. Riemann (1996)
Evidence that the local effect of ultraviolet radiation on the growth of murine melanomas is immunologically mediated.
C. Donawho (1991)
Effects of ultraviolet radiation on murine epidermal Langerhans cells: doses of ultraviolet radiation that modulate ICAM-1 (CD54) expression and inhibit Langerhans cell function cause delayed cytotoxicity in vitro.
A. Tang (1992)
Effect of IL-12 on immune suppression and suppressor cell induction by ultraviolet radiation.
D. Schmitt (1995)
Activated Complement Component 3 (C3) Is Required for Ultraviolet Induction of Immunosuppression and Antigenic Tolerance
C. Hammerberg (1998)
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F. Strickland (2001)
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A. Mittal (2003)
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S. K. Mantena (2005)
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S. Sato (2008)
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N. Yusuf (2007)
Natural Health Products, Modulation of Immune Function and Prevention of Chronic Diseases
P. Haddad (2005)
Skin alterations and diseases in advanced age
E. Makrantonaki (2008)
Signal transduction pathways: targets for chemoprevention of skin cancer.
A. Bode (2000)
Impact of oral green tea catechins on UVR-induced skin inflammation
G. Darby (2014)
Formulating a Day Cream with SPF: A Case Study
Anne Pouillot (2016)
Molecular mechanisms of green tea polyphenols with protective effects against skin photoaging
E. Roh (2017)
Ultraviolet a irradiation of C57BL/6 mice suppresses systemic contact hypersensitivity or enhances secondary immunity depending on dose.
S. Byrne (2002)
In vitro therapeutic effect of epigallocatechin gallate on nicotine-induced impairment of resistance to Legionella pneumophila infection of established MH-S alveolar macrophages.
K. Matsunaga (2002)
Polyphenols extracted from the Green Tea ( Camellia sinensis ) augments the protective immune responses in mice challanged with Salmonella typhimurium
Tri Ratnaningsih (2004)
Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice
R. Yi (2019)
Green Tea ( Camellia Sinensis ) Ordinary Beverages or Medicinal Beverages: A Review
Jalal Omidi (2019)
Alpha-phellandrene promotes immune responses in normal mice through enhancing macrophage phagocytosis and natural killer cell activities.
Jen-Jyh Lin (2013)
Botanical Antioxidants for Skin Protection: An Overview
F. Afaq (2010)
Green tea and skin cancer: photoimmunology, angiogenesis and DNA repair.
S. Katiyar (2007)
Neutrophil Restraint by Green Tea: Inhibition of Inflammation, Associated Angiogenesis, and Pulmonary Fibrosis1
M. Donà (2003)
Protective effect of green tea polyphenols against ultraviolet B-induced damage to HaCaT cells
Liang-yu Wu (2011)
Differential regulation of nuclear factor‐kappa B subunits on epidermal keratinocytes by ultraviolet B and tacrolimus
Ching-Shuang Wu (2012)
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T. Zhou (2018)
Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress.
S. Katiyar (2001)
Botanicals and Cosmeceuticals for Sun Protection
W. Tuong (2015)
Silymarin, a Flavonoid from Milk Thistle (Silybum marianum L.), Inhibits UV‐induced Oxidative Stress Through Targeting Infiltrating CD11b+ Cells in Mouse Skin †
S. Katiyar (2008)
Green tea treatment of ultraviolet-B (UVB) skin carcinogenesis in mice
M. I. El-Sherry (2007)
Ultraviolet Light B-Mediated Inhibition of Skin Catalase Activity Promotes Gr-1+CD11b+ Myeloid Cell Expansion
N. J. Sullivan (2012)
Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion markers in CD-1 mouse skin by oleandrin.
F. Afaq (2004)
Anti-Aging Skin Care Ingredient Technologies
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