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Dietary Choline Restriction Causes Complex I Dysfunction And Increased H(2)O(2) Generation In Liver Mitochondria.
K. Hensley, Y. Kotake, H. Sang, Q. Pye, G. Wallis, L. Kolker, T. Tabatabaie, C. Stewart, Y. Konishi, D. Nakae, R. Floyd
Published 2000 · Biology, Medicine
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Removal of choline from the diet results in accumulation of triglycerides in the liver, and chronic dietary deficiency produces a non-genotoxic model of hepatocellular carcinoma. An early event in choline deficiency is the appearance of oxidized lipid, DNA and protein, suggesting that increased oxidative stress may facilitate neoplasia in the choline deficient liver. In this study, we find that mitochondria isolated from rats fed a choline-deficient, L-amino acid defined diet (CDAA) demonstrate impaired respiratory function, particularly in regard to complex I-linked (NADH-dependent) respiration. This impairment in mitochondrial electron transport occurs coincidentally with alterations in phosphatidylcholine metabolism as indicated by an increased ratio of long-chain to short-chain mitochondrial phosphatidylcholine. Moreover, hydrogen peroxide (H(2)O(2)) generation is significantly increased in mitochondria isolated from CDAA rats compared with mitochondrial from normal rats, and the NADH-specific yield of H(2)O(2) is increased by at least 2.5-fold. These findings suggest an explanation for the rapid onset of oxidative stress and energy compromise in the choline deficiency model of hepatocellular carcinoma and indicate that dietary choline withdrawal may be a useful paradigm for the study of mitochondrial pathophysiology in carcinogenesis.
This paper references
The Occurrence of Neoplasms in the Liver, Lungs, and Other Tissues of Rats as a Result of Prolonged Choline Deficiency.
D. H. Copeland (1946)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
Protein be a useful tool for the general study of mitochondrial function measurement with the folin phenol reagent
A. Hayashi (1951)
The content of adenosine polyphosphates in fatty PTEN, a putative protein tyrosine phosphatase gene mutated in human livers
M U Dianzani (1956)
The content of adenosine polyphosphates in fatty livers.
M. Dianzani (1957)
Studies on the electron-transport system. 27. The respiratory activity of acetoneextracted beef-heart mitochondria: role of coenzyme Q and other lipids.
R. Lester (1961)
Studies on the electron transportdeficiency
R. L. Lester (1961)
Studies on the electron transport deficiency
R L Lester (1961)
Studies of the electron-transfer system. XLIX. Sites of phospholipid involvement in the electron-transfer chain.
G. Brierley (1962)
Studies of the electron
G. P. Brierley (1962)
Hepatic ATP and triglyceride levels in choline-deficient rats with and without dietary orotic acid supplementation.
J. Simon (1969)
levels in choline deficient rats with and without dietary orotic acid Regulation of G1 progression supplementation by the PTEN tumor suppressor protein is linked to inhibition of the 31
J B Simon (1969)
Role of lipoperoxidation in early choline deficiency.
A. Ghoshal (1970)
The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen.
A. Boveris (1973)
Mitochondrial H2O2 formation: Relationship with energy conservation
G. Loschen (1973)
Optimization of methods for aspartate aminotransferase and alanine aminotransferase.
H. U. Bergmeyer (1978)
Optimization of phosphatidylcholine concentrations in the serum of patients with mammary
K. I. Bergmeyer (1978)
Optimization of phosphatidylcholine concentrations in the serum of patients with mammary methods for aspartate aminotransferase and alanine aminotransferase
K I Bergmeyer (1978)
The rapid induction of liver cell death in rats fed a choline-deficient methionine-low diet.
A. Ghoshal (1983)
The effect of choline deficiency on the outer membranes of rat liver mitochondria.
C. Filipowicz (1983)
Rapid lipid peroxidation in the nuclear fraction of rat liver induced by a diet deficient in choline and methionine.
T. Rushmore (1984)
The induction of liver cancer by dietary deficiency of choline and methionine without added carcinogens.
A. Ghoshal (1984)
Rapid lipid J. Biol. Chem
T H Rushmore (1984)
Rapid lipid J
T. H. Rushmore (1984)
T. H. Rushmore (1984)
Probable free radical effects on rat liver nuclei during early hepatocarcinogenesis with a choline-devoid low methionine diet.
T. Rushmore (1987)
Structural analysis of choline phospholipids by fast atom bombardment mass spectrometry and tandem mass spectrometry.
A. Hayashi (1989)
and specifically for the study of lipid influence on mitochondrial Structural analysis of choline phospholipids by fast atom bombardmentROS production . mass spectrometry and tandem mass spectrometry
F. M. Yakes (1989)
Production of Both 8‐Hydroxydeoxyguanosine in Liver DNA and γ‐Glutamyltransferase‐positive Hepatocellular Lesions in Rats Given a Choline‐deficient, l‐Amino Acid‐defined Diet
D. Nakae (1990)
The role of 8-hydroxyguanine in carcinogenesis.
R. Floyd (1990)
Evidence that remodeling of the fatty acids of phosphatidylcholine is regulated in isolated rat hepatocytes and involves both the sn-1 and sn-2 positions.
L. Tijburg (1991)
Selective inhibition of NADH-CoQ oxidoreductase (complex I) of rat brain mitochondria by arachidonic acid.
H. Morii (1991)
Selective inhibition of 34 Variations in energy and phospholipid metabolism in normal and cancer human mammary epithelial NADH-CoQ oxidoreductase (complex I) of rat brain mitochondria by cells
H Morii (1991)
Selective inhibition of 34.Ting,Y.L., Sherr,D. and Degani,H
H. Morii (1991)
Inhibitory effect of dietary iron deficiency on inductions of putative preneoplastic lesions as well as 8-hydroxydeoxyguanosine in DNA and lipid peroxidation in the livers of rats caused by exposure to a choline-deficient L-amino acid defined diet.
H. Yoshiji (1992)
Alterations in nucleotide pools in rats fed diets deficient in choline, methionine and/or folic acid.
S. James (1992)
Alterations in nucleotide 37 pools in rats fed diets deficient in choline, methionine and/or folic acid
S J James (1992)
Prevention by acetylsalicylic acid of liver cirrhosis and carcinogenesis as well as generations of 8-hydroxydeoxyguanosine and thiobarbituric acid-reactive substances caused by a choline-deficient, L-amino acid-defined diet in rats.
A. Denda (1994)
Pyruvate utilization, phosphocholine and adenosine triphosphate (ATP) are markers of human breast tumor progression: a 31P- and 13C-nuclear magnetic resonance (NMR) spectroscopy study.
S. Singer (1995)
New insight into the biochemical pathology of liver in choline deficiency.
A. Ghoshal (1995)
Pyruvate utilization, sulfenic acid intermediate and implications for redox regulation. phosphocholine and adenosine triphosphate (ATP) are markers of human Biochemistry
S Singer (1995)
New insight into the biochemical pathology of liver in choline deficiency transport system XLIX. Sites of phospholipid involvement in the electron transfer chain
A K Ghoshal (1995)
Cancer Cell Cycles
C. Sherr (1996)
Variations in energy and phospholipid metabolism in normal and cancer human mammary epithelial cells.
Y. Ting (1996)
Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.
R. Katz-Brull (1996)
Phosphocholine and choline content of rat sarcoma cells grown in the presence and absence of serum.
T. Smith (1996)
Kinetics of choline transport and triphosphate
R. Katz-Brull (1996)
Food Factors for Cancer Prevention
H. Ohigashi (1997)
Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA damage in human cells following oxidative stress.
F. Yakes (1997)
Direct Inhibition of Mitochondrial Respiratory Chain Complex III by Cell-permeable Ceramide*
T. Gudz (1997)
PTEN, a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate Cancer
J. Li (1997)
Interaction of α‐Phenyl‐N‐tert‐Butyl Nitrone and Alternative Electron Acceptors with Complex I Indicates a Substrate Reduction Site Upstream from the Rotenone Binding Site
K. Hensley (1998)
Specific and reversible inactivation of protein tyrosine phosphatases by hydrogen peroxide: evidence for a sulfenic acid intermediate and implications for redox regulation.
J. Denu (1998)
Alterations of phosphatidylcholine concentrations in the serum of patients with mammary cancer.
J. Hradec (1998)
The Tumor Suppressor, PTEN/MMAC1, Dephosphorylates the Lipid Second Messenger, Phosphatidylinositol 3,4,5-Trisphosphate*
T. Maehama (1998)
Inhibition by phenyl N-tert-butyl nitrone of early phase carcinogenesis in the livers of rats fed a choline-deficient, L-amino acid-defined diet.
D. Nakae (1998)
Specific and reversible inactivation of zero trans method
J. M. Denu (1998)
breast tumor progression: A 31 P and 13 C nuclear magnetic resonance Received April 2NMR) spectroscopy study
Regulation of G 1 progressionsupplementation
T. M. Roberts (1999)
and phosphatidylinositiol 3 - kinase / Akt pathway
T. A. D. Smith
levels in choline deficient rats with and without dietary orotic acid
Mitochondrial defects in choline deficiency
This paper is referenced by
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P. Middleton (2021)
Modeling Diet-Induced NAFLD and NASH in Rats: A Comprehensive Review
Lydie Carreres (2021)
Hepatoxicity mechanism of cantharidin-induced liver LO2 cells by LC-MS metabolomics combined traditional approaches.
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The Microbiota–Gut–Brain Axis Heart Shunt Part I: The French Paradox, Heart Disease and the Microbiota
M. Obrenovich (2020)
Relevance of reactive oxygen species in liver disease observed in transgenic mice expressing the hepatitis B virus X protein
dAE-yEUL yU (2020)
Investigating fibrosis and inflammation in an ex vivo NASH murine model.
E. Gore (2020)
Dietary choline improves growth performance, antioxidant ability and reduces lipid metabolites in practical diet for juvenile Pacific white shrimp, Litopenaeus vannamei
B. Shi (2020)
Comparative effects of nanoemulsions loaded with duck oil and lard oil on palmitate-induced lipotoxicity.
E. S. Kang (2019)
Health Benefits of Carotenoids: A Role of Carotenoids in the Prevention of Non-Alcoholic Fatty Liver Disease
Yoojin Lee (2019)
Nanoemulsions improve the efficacy of turmeric in palmitate- and high fat diet-induced cellular and animal models.
Eun Ji Lee (2019)
LINC00116 codes for a mitochondrial peptide linking respiration and lipid metabolism
A. Chugunova (2019)
Impact of 17β-estradiol on complex I kinetics and H2O2 production in liver and skeletal muscle mitochondria
M. Torres (2018)
Mitochondrial role in the neonatal predisposition to developing nonalcoholic fatty liver disease
P. Baker (2018)
Role of Oxidative Stress in Pathophysiology of Nonalcoholic Fatty Liver Disease
M. Masarone (2018)
Modeling the Western Diet for Preclinical Investigations.
K. Hintze (2018)
Zanthoxylum ailanthoides Suppresses Oleic Acid-Induced Lipid Accumulation through an Activation of LKB1/AMPK Pathway in HepG2 Cells
Eun-Bin Kwon (2018)
Targeting Oxidative Stress for the Treatment of Liver Fibrosis.
T. Luangmonkong (2018)
Resveratrol improves high-fat diet induced fatty liver and insulin resistance by concomitantly inhibiting proteolytic cleavage of sterol regulatory element-binding proteins, free fatty acid oxidation, and intestinal triglyceride absorption.
Eman F. Khaleel (2018)
Reviews of Physiology, Biochemistry and Pharmacology, Vol. 175
B. Nilius (2018)
Mitochondria in Liver Diseases
I. Grattagliano (2018)
Regulation of lipid peroxidation and ferroptosis in diverse species.
M. Conrad (2018)
Mitochondrial adaptation in steatotic mice.
Claudia Einer (2018)
Effect of a normal protein diet on oxidative stress and organ damage in malnourished rats
H. Gourine (2018)
1 Hepatocellular Carcinoma : Epidemiology and Etiology
Pres Universud-Paris (2017)
The Differentiation of Intestinal-Failure-Associated Liver Disease from Nonalcoholic Fatty Liver and Nonalcoholic Steatohepatitis.
A. Buchman (2017)
A computational model of hepatic energy metabolism: Understanding the role of zonation in the development and treatment of non-alcoholic fatty liver disease (NAFLD).
W. B. Ashworth (2017)
1 Hepatocellular Carcinoma : Epidemiology and Etiology
D. Esposti (2017)
Mitochondrial adaptations to dietary lipids in liver steatosis prevent hepatotoxicity but sensitize to secondary liver insult
Claudia Einer (2017)
Influence of hyperglycemia on liver inflammatory conditions in the early phase of non‐alcoholic fatty liver disease in mice
S. Harada (2017)
Impacto del balance energético negativo sobre la función mitocondrial en el hígado bovino
García-Roche Saracco (2017)
Excessive alcohol consumption induces methane production in humans and rats
E. Tuboly (2017)
Diet high in fructose promotes liver steatosis and hepatocyte apoptosis in C57BL/6J female mice: Role of disturbed lipid homeostasis and increased oxidative stress.
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