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Apoptosis Of Ejaculated Human Sperm Is Induced By Co-incubation With Chlamydia Trachomatis Lipopolysaccharide.

A. Eley, S. Hosseinzadeh, H. Hakimi, I. Geary, A. Pacey
Published 2005 · Biology, Medicine

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BACKGROUND Previous work has shown that co-incubation of human sperm with Chlamydia trachomatis serovars E and LGV leads to premature sperm death and that this is due primarily to chlamydial lipopolysaccharide (LPS). Here, we investigated the possible involvement of apoptosis in this premature sperm death. METHODS Highly motile preparations of sperm from normozoospermic patients were co-incubated for 6 h with extracted LPS from C. trachomatis serovars E and LGV. Three different methods were used to determine if LPS-treated sperm underwent apoptosis, including: (i) flow cytometry; (ii) measurement of ADP:ATP ratios; and (iii) measurement of mono- and oligonucleosomal DNA fragments. Caspase activity was also investigated by fluorimetry and by use of a pan-caspase inhibitor and caspase-3 inhibitor. RESULTS All three methods used for detection indicated that C. trachomatis LPS induced some apoptosis in sperm after 6 h when compared with a staurosporine (apoptosis-positive) control. Moreover, a greater degree of apoptosis was seen with C. trachomatis serovar E than with serovar LGV. It was also shown that C. trachomatis LPS-induced apoptosis of sperm could be blocked with a pan-caspase inhibitor and a caspase-3 inhibitor. Moreover, by using a fluorogenic substrate, apoptosis was shown to be caspase-mediated. CONCLUSIONS In general it is believed that apoptosis does not occur in C. trachomatis-infected host cells. However, using three different methods, our findings clearly indicate that co-incubation of sperm with C. trachomatis LPS results in cellular death which is in part due to apoptosis and is caspase-mediated. These findings provide an explanation as to how C. trachomatis can mediate premature death in human sperm.
This paper references
Laboratory manual for the examination of human semen and semen-cervical mucus interaction.
Belsey Ma (1980)
10.1016/S0015-0282(16)49525-7
Endotoxin-polluted medium in a human in vitro fertilization program.
E. Snyman (1986)
10.1016/S0015-0282(16)59659-9
Endotoxins in culture medium for human in vitro fertilization.
S. Fishel (1988)
Biology of Chlamydia trachomatis
J Schachter (1990)
10.1136/sti.69.3.239
Epidemiology of Chlamydia trachomatis using nested PCR.
A. Eley (1993)
10.1128/iai.61.12.5044-5048.1993
In vivo induction of apoptosis (programmed cell death) in mouse thymus by administration of lipopolysaccharide
Y. Zhang (1993)
10.1006/EXCR.1993.1182
Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells.
W. Gorczyca (1993)
10.1016/S0015-0282(16)57673-0
Spermatozoa selected by a discontinuous Percoll density gradient exhibit better motion characteristics, more hyperactivation, and longer survival than direct swim-up.
J. Moohan (1995)
THE IMPACT OF INFECTION ON SPERM QUALITY
K. Purvis (1996)
Apoptosis in human ejaculated sperm cells (notulae seminologicae 9).
B. Baccetti (1996)
Chlamydia and its effect on reproduction.
L. Westrom (1996)
10.1074/JBC.273.12.6736
Type I cAMP-dependent Protein Kinase Delays Apoptosis in Human Neutrophils at a Site Upstream of Caspase-3*
L. K. Parvathenani (1998)
Lipopolysaccharide and ceramide use divergent signaling pathways to induce cell death in murine macrophages.
V. Lakics (1998)
10.1084/JEM.187.4.487
Inhibition of Apoptosis in Chlamydia-infected Cells: Blockade of Mitochondrial Cytochrome c Release and Caspase Activation
T. Fan (1998)
10.1016/S0934-8840(98)80095-9
Induction of apoptosis by Chlamydia psittaci and Chlamydia trachomatis infection in tissue culture cells.
D. Gibellini (1998)
Apoptosis of epithelial cells and macrophages due to infection with the obligate intracellular pathogen Chlamydia psittaci.
D. Ojcius (1998)
10.1006/EXCR.1999.4586
Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway.
D. Sakkas (1999)
10.1016/S0015-0282(00)00623-3
Measuring apoptosis in human spermatozoa: a biological assay for semen quality?
G. J. Oosterhuis (2000)
10.1128/IAI.68.9.4872-4876.2000
Coincubation of Human Spermatozoa with Chlamydia trachomatis In Vitro Causes Increased Tyrosine Phosphorylation of Sperm Proteins
S. Hosseinzadeh (2000)
10.1002/J.1939-4640.2000.TB03272.X
World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 4th ed.
D. Lamb (2000)
Morphological and biochemical analysis of cell death in human ejaculated spermatozoa.
G. Blanc-Layrac (2000)
10.1016/S0022-1759(00)00178-2
Measurement of the ADP:ATP ratio in human leukaemic cell lines can be used as an indicator of cell viability, necrosis and apoptosis.
D. Bradbury (2000)
10.1128/IAI.69.12.7880-7888.2001
Epithelial Cells Infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) Are Resistant to Apoptosis
K. Rajalingam (2001)
10.1128/IAI.69.4.2442-2447.2001
Persistent Chlamydia trachomatisInfections Resist Apoptotic Stimuli
D. Dean (2001)
10.1152/AJPCELL.2001.280.1.C81
Modulation of P2Z/P2X(7) receptor activity in macrophages infected with Chlamydia psittaci.
R. Coutinho-Silva (2001)
10.1152/AJPGI.2001.281.3.G726
Helicobacter pylori lipopolysaccharide induces apoptosis of cultured guinea pig gastric mucosal cells.
T. Kawahara (2001)
10.1128/CDLI.8.1.206-208.2001
Porins and Lipopolysaccharide Induce Apoptosis in Human Spermatozoa
F. Gorga (2001)
10.1093/HUMREP/16.2.293
Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death.
S. Hosseinzadeh (2001)
10.1093/MOLEHR/8.11.984
Caspase activity and apoptotic markers in ejaculated human sperm.
S. Weng (2002)
10.1093/HUMREP/17.5.1266
Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality.
Han-Ming Shen (2002)
10.1093/HUMREP/17.10.2665
Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes.
G. Ricci (2002)
Detection of apoptotic alterations in sperm in subfertile patients and their correlation with sperm
HM Shen (2002)
10.1002/J.1939-4640.2003.TB02669.X
Deterioration of plasma membrane is associated with activated caspases in human spermatozoa.
U. Paasch (2003)
10.1099/JMM.0.04836-0
Chlamydia trachomatis-induced death of human spermatozoa is caused primarily by lipopolysaccharide.
S. Hosseinzadeh (2003)
10.1046/J.1432-1033.2003.03392.X
Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC.
H. Heine (2003)
10.1095/biolreprod.102.012500
Caspase-Independent Exposure of Aminophospholipids and Tyrosine Phosphorylation in Bicarbonate Responsive Human Sperm Cells1
K. J. de Vries (2003)
10.1074/JBC.M211275200
Role of Proapoptotic BAX in Propagation of Chlamydia muridarum (the Mouse Pneumonitis Strain of Chlamydia trachomatis) and the Host Inflammatory Response*
J. Perfettini (2003)
10.1016/J.FERTNSTERT.2003.09.030
Activation pattern of caspases in human spermatozoa.
U. Paasch (2004)
10.1095/biolreprod.103.025627
Cryopreservation and Thawing Is Associated with Varying Extent of Activation of Apoptotic Machinery in Subsets of Ejaculated Human Spermatozoa1
U. Paasch (2004)
10.1128/IAI.72.1.451-460.2004
Chlamydia-Infected Cells Continue To Undergo Mitosis and Resist Induction of Apoptosis
W. Greene (2004)
10.1038/nrmicro1007
Chlamydia and apoptosis: life and death decisions of an intracellular pathogen
G. Byrne (2004)
10.1093/HUMUPD/DMH003
Role of caspases in male infertility.
T. Said (2004)
A (2004b) Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa
U Paasch (2004)
10.1007/BF02017711
Fallopian tube obstruction as a sequela toChlamydia trachomatis infection
M. Kosseim (2005)



This paper is referenced by
10.1098/rspb.2020.0302
Mating changes the genital microbiome in both sexes of the common bedbug Cimex lectularius across populations
Sara Bellinvia (2020)
10.1111/een.12784
Bacterial communities of the reproductive organs of virgin and mated common bedbugs, Cimex lectularius
Sara Bellinvia (2020)
10.1093/femsre/fuaa043
Host cell death during infection with Chlamydia: a double-edged sword
B. S. Sixt (2020)
10.1111/and.13743
The role of infections and leukocytes in male infertility
R. Henkel (2020)
10.3390/microorganisms8010136
Waddlia chondrophila and Male Infertility
D. Baud (2020)
10.1101/2020.10.21.349811
The Effect of post Chlamydia Trachomatis Infection treatment on Reactive Oxygen Species and Sperm Parameters of Infertile Men
Reza Azmakan (2020)
10.1111/andr.12625
Semen quality is affected by HLA class I alleles together with sexually transmitted diseases
P. I. Marques (2019)
10.5812/JJM.84954
Investigating Chlamydia trachomatis and Genital Mycoplasma Prevalence and Apoptosis Markers in Infertile and Fertile Couples
M. Moosavian (2019)
10.1101/819300
Mating changes the genital microbiome in both sexes of the common bedbug Cimex lectularius across populations
Sara Bellinvia (2019)
10.1111/andr.12596
Alpha lipoic acid reverses the negative effect of LPS on mouse spermatozoa and developmental competence of resultant embryos in vitro
A. Makvandi (2019)
10.1186/s12866-019-1392-z
Interaction of different Chlamydiae species with bovine spermatozoa
T. Eckert (2019)
10.3390/ani9100719
Implication of Polyhistidine, a Novel Apoptosis Inhibitor, in Inhibiting Lipopolysaccharide-Induced Apoptosis in Boar Sperm
Tian-zeng Song (2019)
10.1016/j.purol.2019.08.261
[Prevalence and role of IgG anti-Chlamydia trachomatis in a population of infertile men in Morocco].
M. Frikh (2019)
10.1038/s41598-019-42396-3
Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells
S. Filardo (2019)
10.1111/and.13093
Implications of prostate inflammation on male fertility
R. Motrich (2018)
10.1177/0956462417735245
The impact of Chlamydia trachomatis infection on sperm parameters and male fertility: A comprehensive study
M. Moazenchi (2018)
10.3389/fcell.2018.00065
Apoptosis Is a Demanding Selective Tool During the Development of Fetal Male Germ Cells
I. Bejarano (2018)
Clinical manifestation of chlamydial urethritis , epididymitis , prostatitis
Young-Suk Lee (2018)
10.5812/IRCMJ.81348
Cytochrome C Oxidase 6B2 Reflects the Mitochondrial Status Through the Oxidative Phosphorylation
M. Hashemitabar (2018)
10.1016/j.nmni.2018.08.012
Male infertility: the intracellular bacterial hypothesis
M. Stojanov (2018)
10.1111/and.12944
Association of asymptomatic Chlamydia trachomatis infection with male infertility and the effect of antibiotic therapy in improvement of semen quality in infected infertile men
M. H. Ahmadi (2018)
10.1111/and.12812
Molecular detection of Chlamydia trachomatis and semen quality of sexual partners of infertile women
M. López-Hurtado (2018)
10.1111/aji.12838
Characterization of microbiota in male infertility cases uncovers differences in seminal hyperviscosity and oligoasthenoteratozoospermia possibly correlated with increased prevalence of infectious bacteria
C. Monteiro (2018)
10.1038/s41598-017-17944-4
Lipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitis
E. J. Silva (2017)
10.1038/s41598-017-12316-4
Erratum: Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
Jenniffer Puerta Suárez (2017)
10.1111/and.12747
Relationship between Chlamydia trachomatis and Mycoplasma genitalium infection and pregnancy rate and outcome in Iranian infertile couples
L. Dehghan Marvast (2017)
10.5653/cerm.2017.44.4.207
Effects of infections with five sexually transmitted pathogens on sperm quality
S. Kim (2017)
Healthcare-seeking behaviour for sexually transmitted infection testing in New Zealand: A mixed methods study
H. Denison (2017)
10.1038/s41598-017-01262-w
Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
Jenniffer Puerta Suárez (2017)
10.5772/63717
Decrease in Sperm Quality due to Infection of Human Papilloma Virus and Chlamydia trachomatis
María del Carmen Colín-Ferreyra (2016)
10.1016/j.jri.2016.08.006
The effect of bacteriospermia and leukocytospermia on conventional and nonconventional semen parameters in healthy young normozoospermic males.
M. Fraczek (2016)
10.1186/s12610-016-0034-7
Gut Endotoxin Leading to a Decline IN Gonadal function (GELDING) - a novel theory for the development of late onset hypogonadism in obese men
K. Tremellen (2016)
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