Online citations, reference lists, and bibliographies.
← Back to Search

Location And Effects Of Long-term NaCl Stress On Superoxide Dismutase And Ascorbate Peroxidase Isoenzymes Of Pea (Pisum Sativum Cv. Puget) Chloroplasts.

J. M. Gomez, A. Jimenez, E. Olmos, F. Sevilla
Published 2004 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The present work describes the intrachloroplast localization and the changes that took place in the thylakoid and stroma-located superoxide dismutases (SOD, EC 1.15.1.1) and ascorbate peroxidases (APX, EC 1.11.1.11), in response to long-term NaCl stress in Pisum sativum L. cv. Puget plants. Native PAGE using high chloroplast protein concentrations pointed to the presence of the two main Fe-SODs, together with CuZn-SODs, both in thylakoids and in the stroma. Western blot and immunogold labelling using the antibodies against chloroplastic Fe-SOD from Nuphar luteum also confirmed the chloroplastic localization of a Fe-SOD. Thylakoidal Fe-SOD activity was induced by a NaCl concentration as low as 70 mM, while CuZn-SOD was induced at 90 mM, although in severe stress conditions (110 mM) both activities were similar to the levels at 90 mM NaCl. NaCl stress also induced stromatic Fe-SOD and CuZn-SOD activities, although these inductions only started at higher NaCl concentration (90 mM) and were significant at 110 mM NaCl. The increase in activity of both Fe-SODs was matched by an increase in Fe-SOD protein. Chloroplastic APX isoenzymes behaved differently in thylakoids and stroma in response to NaCl. A significant increase of stromal APX occurred at 70 mM, whereas the thylakoidal APX activity was significantly and progressively lost in response to NaCl stress (70-110 mM). A significant increase in the H2O2 content of chloroplasts during stress and a reduction in the ascorbate level at 90 mM NaCl also took place, although the oxidized ascorbate pool at the highest NaCl concentration did not show significant changes. These results suggest that the loss of thylakoidal APX may be an important factor in the increase in chloroplastic H2O2, which also results from the increased thylakoid and stroma-located Fe-SOD and CuZn-SOD activities. This H2O2 may be involved in the induction of stromal APX. The up-regulation of the above enzymes in the described stress conditions would contribute to the adaptation of cv. Puget plants to moderate NaCl stress.
This paper references
Enhancement of oxidative stress tolerance in transgenic tobacco overexpressing antioxidant enzymes
L. Slooten (1995)
10.1016/S0891-5849(97)00107-X
Use of transgenic plants to study antioxidant defenses.
R. D. Allen (1997)
10.1271/BBB.63.302
Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.).
H. Kaminaka (1999)
Mechanisms of oxygen activation in different compartments of plant cells
E. Elstner (1991)
10.1046/J.1469-8137.1999.00341.X
Response of antioxidant systems and leaf water relations to NaCl stress in pea plants
J. A. Hernández (1999)
10.1016/S0176-1617(99)80019-4
Antioxidative Responses of Shoots and Roots of Wheat to Increasing NaCI Concentrations
S. Meneguzzo (1999)
10.1111/J.1365-313X.1994.00397.X
Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought.
R. Mittler (1994)
10.1016/S0176-1617(11)82142-5
Induction of Several Antioxidant Enzymes in the Selection of a Salt-Tolerant Cell Line of Pisum sativum
E. Olmos (1994)
10.1111/J.1399-3054.1994.TB00397.X
Characterization of an iron‐containing superoxide dismutase from a higher plant, Citrus limonum
M. Almansa (1994)
10.1093/OXFORDJOURNALS.PCP.A078288
Thylakoid-Bound Ascorbate Peroxidase in Spinach Chloroplasts and Photoreduction of Its Primary Oxidation Product Monodehydroascorbate Radicals in Thylakoids
C. Miyake (1992)
10.1007/s004250050215
Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in Citrus
Yardena Gueta-Dahan (1997)
In: Foyer CH, Mullineaux PM, eds
CH Foyer (1994)
10.1104/pp.108.3.1151
Peroxisomal Copper,Zinc Superoxide Dismutase (Characterization of the Isoenzyme from Watermelon Cotyledons)
P. Bueno (1995)
10.1080/10715769900301261
Differential response of antioxidative enzymes of chloroplasts and mitochondria to long-term NaCl stress of pea plants.
J. M. Gomez (1999)
10.1034/J.1399-3054.1998.1040431.X
Stromal and thylakoid‐bound ascorbate peroxidases in NaCl‐treated wheat
S. Meneguzzo (1998)
10.1093/JEXBOT/53.372.1255
Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes.
L. D. Del Rio (2002)
10.1016/0003-2697(71)90370-8
Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.
C. Beauchamp (1971)
10.1016/0168-9452(94)04047-8
Salt-induced oxidative stress in chloroplasts of pea plants
J. A. Hernández (1995)
10.1093/OXFORDJOURNALS.PCP.A029285
Molecular characterization and physiological role of a glyoxysome-bound ascorbate peroxidase from spinach.
T. Ishikawa (1998)
10.1023/A:1005858120232
Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress
Rebecca G. Stevens (2004)
10.1201/9781351070454
Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants
C. Foyer (1993)
10.1007/s004250050237
Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli
C. R. Caldwell (1997)
10.1016/s0021-9258(19)36683-9
Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase.
R. Mittler (1992)
10.1104/PP.010188
Antioxidant systems and O(2)(.-)/H(2)O(2) production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins.
J. Hernández (2001)
In: Foyer CH, Mullineaux PM, eds. Cause of photooxidative stress and amelioration of defense systems of plants
Ch Foyer (1994)
10.3109/10715769109145801
Purification of an iron-containing superoxide dismutase from a citrus plant, Citrus limonum R.
M. S. Almansa (1991)
10.1007/s000180050041
Dual action of the active oxygen species during plant stress responses
J. Dat (2000)
Puri®cation and molecular
C Miyake (1993)
10.1016/S0176-1617(84)80130-3
Superoxide Dismutases from a Citrus Plant: Presence of Two Iron-containing Isoenzymes in Leaves of Lemon Trees (Citrus limonum L.).
F. Sevilla (1984)
10.1093/OXFORDJOURNALS.PCP.A028963
Inactivation Mechanism of Ascorbate Peroxidase at Low Concentrations of Ascorbate; Hydrogen Peroxide Decomposes Compound I of Ascorbate Peroxidase
C. Miyake (1996)
10.1007/BF00197587
Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family
M. Santos (2004)
10.1104/pp.111.4.1177
Water-Deficit Tolerance and Field Performance of Transgenic Alfalfa Overexpressing Superoxide Dismutase
B. Mckersie (1996)
10.1080/10715769900301251
Superoxide and hydroxyl radical generation, and superoxide dismutase in PSII membrane fragments from wheat.
F. Navari-Izzo (1999)
10.1111/J.1399-3054.1993.TB01792.X
Salt-induced oxidative stress mediated by activated oxygen species in pea leaf mitochondria
J. A. Hernández (1993)
10.1111/J.1432-1033.1985.TB08673.X
Isolation and characterization of an iron-containing superoxide dismutase from tomato leaves, Lycopersicon esculentum.
J. Kwiatowski (1985)
10.1046/J.1365-3040.2000.00602.X
Tolerance of pea (Pisum sativum L.) to long‐term salt stress is associated with induction of antioxidant defences
J. A. Hernández (2000)
10.1104/pp.112.4.1703
Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Plants Overproducing Fe-Superoxide Dismutase in Chloroplasts
W. Van Camp (1996)
10.1093/PCP/41.3.311
Evaluation of the defense system in chloroplasts to photooxidative stress caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli.
Y. Miyagawa (2000)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1104/pp.114.1.275
Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves
A. Jiménez (1997)
10.1126/SCIENCE.284.5414.654
Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis.
S. Karpiński (1999)
10.1093/OXFORDJOURNALS.PCP.A029599
Overexpression of an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress.
J. Wang (1999)
10.1093/OXFORDJOURNALS.PCP.A029557
Induction of Rice Cytosolic Ascorbate Peroxidase mRNA by Oxidative Stress; the Involvement of Hydrogen Peroxide in Oxidative Stress Signalling
S. Morita (1999)
10.1016/0168-9452(87)90213-5
Localization of manganese superoxide dismutase in peroxisomes isolated from Pisum sativum L.
L. M. Sandalio (1987)
10.1016/S0005-2728(00)00256-5
Chloroplastic ascorbate peroxidase is the primary target of methylviologen-induced photooxidative stress in spinach leaves: its relevance to monodehydroascorbate radical detected with in vivo ESR.
J. Mano (2001)
10.1104/pp.113.1.249
Responses of Antioxidants to Paraquat in Pea Leaves (Relationships to Resistance)
J. Donahue (1997)
10.1105/tpc.12.3.319
Proteomics of the Chloroplast: Systematic Identification and Targeting Analysis of Lumenal and Peripheral Thylakoid Proteins
Jean-Benoît Peltier (2000)
10.1034/J.1399-3054.1998.1040424.X
Mitochondrial and peroxisomal ascorbate peroxidase of pea leaves
A. Jimenez (1998)
10.1093/OXFORDJOURNALS.PCP.A029572
Overproduction of Arabidopsis thaliana FeSOD confers oxidative stress tolerance to transgenic maize.
F. Van Breusegem (1999)
10.1104/PP.69.1.161
Isolation and Characterization of an Iron-Containing Superoxide Dismutase From Water Lily, Nuphar luteum.
Marvin L. Salin (1982)
10.1007/BF00388061
Developmental studies on microbodies in wheat leaves
Jürgen Feierabend (2004)
10.1046/j.1365-313x.1998.00294.x
Ozone-induced oxidative burst in the ozone biomonitor plant, tobacco Bel W3.
M. Schraudner (1998)
10.1093/OXFORDJOURNALS.PCP.A078497
Purification and Molecular Properties of the Thylakoid-Bound Ascorbate Peroxidase in Spinach Chloroplasts
C. Miyake (1993)
Manganese superoxide dismutase from a higher plant: puri®cation of a new manganese containing enzyme
F Sevilla (1980)
10.1007/BF00582359
Manganese superoxide dismutase from a higher plant
F. Sevilla (2004)
Identi®cation of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli
Ch Caldwell (1998)
10.1034/J.1399-3054.1998.1040416.X
Thylakoid-bound and stromal antioxidative enzymes in wheat treated with excess copper
F. Navari-Izzo (1998)
10.1016/S0014-5793(98)00483-9
Inhibition of ascorbate peroxidase under oxidative stress in tobacco having bacterial catalase in chloroplasts
T. Shikanai (1998)
10.1093/JEXBOT/53.372.1331
Role of superoxide dismutases (SODs) in controlling oxidative stress in plants.
R. Alscher (2002)
10.1093/OXFORDJOURNALS.PCP.A077844
Ascorbate Peroxidase in Tea Leaves: Occurrence of Two Isozymes and the Differences in Their Enzymatic and Molecular Properties
Gong-Xiang Chen (1989)
10.1034/J.1399-3054.2000.110106.X
Activities of SOD and the ascorbate-glutathione cycle enzymes in subcellular compartments in leaves and roots of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii
V. Mittova (2000)
Identi ® cation of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E . coli
CH Caldwell (1998)
10.1104/pp.110.2.589
Ascorbate Peroxidase (A Prominent Membrane Protein in Oilseed Glyoxysomes)
J. Bunkelmann (1996)
10.1093/OXFORDJOURNALS.PCP.A078795
Attachment of CuZn-Superoxide Dismutase to Thylakoid Membranes at the Site of Superoxide Generation (PSI) in Spinach Chloroplasts: Detection by Immuno-Gold Labeling After Rapid Freezing and Substitution Method
K. Ogawa (1995)
Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves: Changes Induced by Leaf Senescence
A. Jimenez (1997)
10.1104/PP.118.4.1327
Role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves
Jiménez (1998)
Puri®cation and characterization of an iron-containing SOD from a eukaryote, Ginkgo biloba
MV Duke (1985)
10.1104/PP.68.2.275
Distribution of iron-containing superoxide dismutase in vascular plants.
S. Bridges (1981)
10.1093/JEXBOT/53.372.1305
Regulation and function of ascorbate peroxidase isoenzymes.
S. Shigeoka (2002)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
10.1016/0098-8472(88)90033-0
Extracellular ascorbic acid and enzyme activities related to ascorbic acid metabolism in Sedum album L. leaves after ozone exposure
F. J. Castillo (1988)
10.1042/BJ3380041
Alternatively spliced mRNA variants of chloroplast ascorbate peroxidase isoenzymes in spinach leaves.
K. Yoshimura (1999)
10.1104/PP.117.2.565
Overexpression of iron superoxide dismutase in transformed poplar modifies the regulation of photosynthesis at low CO2 partial pressures or following exposure to the prooxidant herbicide methyl viologen.
A. C. Arisi (1998)
10.1006/ABIO.1993.1366
Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium.
R. Mittler (1993)
10.1093/OXFORDJOURNALS.PCP.A029096
Generation of superoxide anion and localization of CuZn-superoxide dismutase in the vascular tissue of spinach hypocotyls: their association with lignification.
K. Ogawa (1997)
10.1093/JXB/ERG091
Mitochondrial and peroxisomal manganese superoxide dismutase: differential expression during leaf senescence.
L. D. Del Rio (2003)
10.2135/CROPSCI1994.0011183X003400030020X
Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton
D. Gossett (1994)
Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice
H Kaminaka (1999)
10.1104/PP.116.4.1195
The activated oxygen role of peroxisomes in senescence
del Río LA (1998)
10.1016/0003-9861(80)90524-X
Isolation and characterization of an iron-containing superoxide dismutase from a eucaryote, Brassica campestris.
Marvin L. Salin (1980)
Puri®cation of an iron-containing superoxide dismutase from a citrus plant
Ms Almansa (1991)
10.1146/ANNUREV.ARPLANT.50.1.601
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons.
K. Asada (1999)
Reactive oxygen species, antioxidant systems
JB Barroso (2002)



This paper is referenced by
10.3390/ijms17010004
Cloning, Characterization and Expression Pattern Analysis of a Cytosolic Copper/Zinc Superoxide Dismutase (SaCSD1) in a Highly Salt Tolerant Mangrove (Sonneratia alba)
Enze Yang (2015)
10.1016/J.JPLPH.2006.03.010
Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities.
M. A. Hoque (2007)
10.1016/J.ENVEXPBOT.2019.02.026
Coordinated responses of mitochondrial antioxidative enzymes, respiratory pathways and metabolism in Arabidopsis thaliana thioredoxin trxo1 mutants under salinity
Antonio Sánchez-Guerrero (2019)
10.1016/J.SAJB.2012.11.005
Effects of salt stress on ion content, antioxidant enzymes and protein profile in different tissues of Broussonetia papyrifera
Zhang Min (2013)
10.1007/s10681-012-0771-4
Sources of high tolerance to salinity in pea (Pisum sativum L.)
A. Leonforte (2012)
10.1007/s11046-007-9000-4
Antioxidative enzymes and isozymes analysis of taro genotypes and their implications in Phytophthora blight disease resistance
Manas Ranjan Sahoo (2007)
10.1007/s10646-016-1696-9
Photosynthesis, antioxidant system and gene expression of bermudagrass in response to low temperature and salt stress
A. Liu (2016)
10.1016/j.ecoenv.2010.05.023
The changes in some biochemical parameters in Zea mays cv. "Martha F1" treated with atrazine.
Gulcin Beker Akbulut (2010)
10.1105/tpc.106.049114
The Arabidopsis aba4-1 Mutant Reveals a Specific Function for Neoxanthin in Protection against Photooxidative Stress[W]
Luca Dall’Osto (2007)
10.1007/s12298-013-0209-z
Effect of salt stress on tomato fruit antioxidant systems depends on fruit development stage
R. Murshed (2013)
10.1016/J.ENVEXPBOT.2010.04.008
Moderate salinity enhances the antioxidative response in the halophyte Hordeum maritimum L. under potassium deficiency
C. Hafsi (2010)
10.1080/09064710802029544
The K/Na replacement and function of antioxidant defence system in sugar beet (Beta vulgaris L.) cultivars
R. Hajiboland (2009)
10.1111/j.1744-7909.2007.00621.x
Rapid inactivation of chloroplastic ascorbate peroxidase is responsible for oxidative modification to Rubisco in tomato (Lycopersicon esculentum) under cadmium stress.
K. Liu (2008)
10.1002/9781119054450.CH7
Salinity and drought stress
M. N. Uddin (2016)
10.1007/s10811-014-0267-9
Effects of the algicides CuSO4 and NaOCl on various physiological parameters in the harmful dinoflagellate Cochlodinium polykrikoides
V. Ebenezer (2014)
10.1016/j.biotechadv.2008.09.003
Biotechnological approach of improving plant salt tolerance using antioxidants as markers.
M. Ashraf (2009)
10.1007/978-3-319-20421-5_2
What Do the Plant Mitochondrial Antioxidant and Redox Systems Have to Say Under Salinity, Drought, and Extreme Temperature?
F. Sevilla (2015)
10.1007/s10681-006-4723-8
Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes
F. Stoddard (2006)
10.1007/s10725-009-9364-1
Allelochemical stress produced by aqueous leachate of Nicotiana plumbaginifolia Viv.
A. Singh (2009)
10.1007/978-3-319-20421-5_12
Modulation of the Ascorbate–Glutathione Cycle Antioxidant Capacity by Posttranslational Modifications Mediated by Nitric Oxide in Abiotic Stress Situations
J. C. Begara-Morales (2015)
10.1016/j.plaphy.2020.04.022
Physiological and quantitative proteomic analysis of NtPRX63-overexpressing tobacco plants revealed that NtPRX63 functions in plant salt resistance.
Liming Lu (2020)
10.1105/tpc.108.061341
A Heterocomplex of Iron Superoxide Dismutases Defends Chloroplast Nucleoids against Oxidative Stress and Is Essential for Chloroplast Development in Arabidopsis[W]
F. Myouga (2008)
10.1111/J.1365-3040.2006.01530.X
The dehydrogenase-mediated recycling of NADPH is a key antioxidant system against salt-induced oxidative stress in olive plants.
R. Valderrama (2006)
10.1007/978-3-319-75088-0
Antioxidants and Antioxidant Enzymes in Higher Plants
D. Gupta (2018)
10.1007/s00299-015-1770-4
Overexpression of CuZnSOD from Arachis hypogaea alleviates salinity and drought stress in tobacco
N. Negi (2015)
10.3389/fpls.2018.01661
Rice Calcineurin B-Like Protein-Interacting Protein Kinase 31 (OsCIPK31) Is Involved in the Development of Panicle Apical Spikelets
Yongbin Peng (2018)
10.22059/JDESERT.2015.54080
Morphophysiological and biochemical changes in tall fescue (Festuca arundinacea Schreb.) under combined salinity and deficit irrigation stresses
R. Manuchehri (2015)
10.1111/j.1365-313X.2009.04000.x
Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity.
G. Tanou (2009)
10.1007/s10535-012-0107-1
Oligogalacturonides stimulate antioxidant system in alfalfa roots
D. Camejo (2012)
Adaptive Response to Salt Stress in Sorghum (Sorghum bicolor)
R. el-Omari (2015)
10.1093/jxb/eru173
Salt stress and senescence: identification of cross-talk regulatory components
A. Allu (2014)
10.1007/s00299-016-1959-1
Elucidation of salt-tolerance metabolic pathways in contrasting rice genotypes and their segregating progenies
P. Mishra (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar